BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P13231 (118 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1HCE-A nrPDB 221 7e-59 1QQK-A nrPDB 33 0.028 6B02-A nrPDB 30 0.30 3P53-A nrPDB 30 0.33 4HWV-A nrPDB 27 2.1 4DPQ-A nrPDB 25 5.2 5UBV-A nrPDB 25 6.1 5YHK-A nrPDB 25 6.4 5EVJ-A nrPDB 25 8.3 6INC-A nrPDB 25 9.5 >1HCE-A nrPDB Length = 118 Score = 221 bits (562), Expect = 7e-59, Method: Compositional matrix adjust. Identities = 118/118 (100%), Positives = 118/118 (100%) Query: 1 MGNRAFKSHHGHFLSAEGEAVKTHHGHHDHHTHFHVENHGGKVALKTHCGKYLSIGDHKQ 60 MGNRAFKSHHGHFLSAEGEAVKTHHGHHDHHTHFHVENHGGKVALKTHCGKYLSIGDHKQ Sbjct: 1 MGNRAFKSHHGHFLSAEGEAVKTHHGHHDHHTHFHVENHGGKVALKTHCGKYLSIGDHKQ 60 Query: 61 VYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHYISADHHGHVSTKEHHDHDTTFEEIII 118 VYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHYISADHHGHVSTKEHHDHDTTFEEIII Sbjct: 61 VYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHYISADHHGHVSTKEHHDHDTTFEEIII 118 >1QQK-A nrPDB Length = 140 Score = 33.1 bits (74), Expect = 0.028, Method: Compositional matrix adjust. Identities = 16/69 (23%), Positives = 34/69 (49%), Gaps = 2/69 (2%) Query: 52 YLSIGDHKQVYLSHHLHGDHSLFHLEHHG-GKVSIKG-HHHHYISADHHGHVSTKEHHDH 109 YL I +V + + +++ + G V+IKG +Y++ + G + K+ + Sbjct: 22 YLRIDKRGKVKGTQEMRNSYNIMEIRTVAVGIVAIKGVESEYYLAMNKEGKLYAKKECNE 81 Query: 110 DTTFEEIII 118 D F+E+I+ Sbjct: 82 DCNFKELIL 90 >6B02-A nrPDB Length = 341 Score = 29.6 bits (65), Expect = 0.30, Method: Compositional matrix adjust. Identities = 13/39 (33%), Positives = 19/39 (48%) Query: 54 SIGDHKQVYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHY 92 SIG H V + D+SLF +E + V K ++ Y Sbjct: 156 SIGQHLDVTMERRQKSDYSLFTIERYNAIVKYKTAYYTY 194 >3P53-A nrPDB Length = 496 Score = 29.6 bits (65), Expect = 0.33, Method: Composition-based stats. Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 3/72 (4%) Query: 5 AFKSHHGHF--LSAEGEAVKTHHGHHDHHTHFHVENHGGKVALKTHCGKYLSIGDHKQVY 62 AF++H G + L+A G V++ + +F +E ++ L+ GK+++ + Q+ Sbjct: 309 AFRTHTGKYWTLTATG-GVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQLA 367 Query: 63 LSHHLHGDHSLF 74 S GD LF Sbjct: 368 ASVETAGDSELF 379 >4HWV-A nrPDB Length = 503 Score = 26.9 bits (58), Expect = 2.1, Method: Composition-based stats. Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 10/56 (17%) Query: 58 HKQVYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHYISADHHGHVSTKEHHDHDTTF 113 H+ V+ + + GD F ++HH G V + H ++ +++H HDHD F Sbjct: 259 HETVHGTDYSGGD---FKVQHHDGLVDVTRHGNYVTLSNNH-------FHDHDKAF 304 >4DPQ-A nrPDB Length = 360 Score = 25.4 bits (54), Expect = 5.2, Method: Composition-based stats. Identities = 12/31 (38%), Positives = 17/31 (54%) Query: 69 GDHSLFHLEHHGGKVSIKGHHHHYISADHHG 99 G H+ H+ + GK SI+G H+ S H G Sbjct: 154 GMHAALHINPYYGKTSIEGLIAHFQSVLHMG 184 >5UBV-A nrPDB Length = 246 Score = 25.4 bits (54), Expect = 6.1, Method: Compositional matrix adjust. Identities = 11/34 (32%), Positives = 17/34 (50%) Query: 23 THHGHHDHHTHFHVENHGGKVALKTHCGKYLSIG 56 T G D H H + + G++A+ H K + IG Sbjct: 159 TRPGRFDRHVHVSLPDVRGRIAILKHHAKKIKIG 192 >5YHK-A nrPDB Length = 238 Score = 25.4 bits (54), Expect = 6.4, Method: Compositional matrix adjust. Identities = 11/45 (24%), Positives = 21/45 (46%) Query: 55 IGDHKQVYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHYISADHHG 99 + D + V+ + G F H +++ G+H H+I+ D G Sbjct: 143 VLDDQPVFRFNQREGVLVGFRTPQHMQGINVAGYHEHFITDDRQG 187 >5EVJ-A nrPDB Length = 387 Score = 25.0 bits (53), Expect = 8.3, Method: Composition-based stats. Identities = 11/23 (47%), Positives = 13/23 (56%) Query: 82 KVSIKGHHHHYISADHHGHVSTK 104 K +I GH H Y DHH V+ K Sbjct: 294 KGTIPGHSHAYDLDDHHRFVTNK 316 >6INC-A nrPDB Length = 259 Score = 24.6 bits (52), Expect = 9.5, Method: Compositional matrix adjust. Identities = 11/45 (24%), Positives = 21/45 (46%) Query: 55 IGDHKQVYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHYISADHHG 99 + D + V+ + G F H +++ G+H H+I+ D G Sbjct: 164 VLDDQPVFRFNQREGVLVGFRTPQHMQGINVAGYHEHFITDDRKG 208 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1HCE-A nrPDB 140 2e-34 Sequences not found previously or not previously below threshold: 3P53-A nrPDB 30 0.24 4HWV-A nrPDB 29 0.43 6B02-A nrPDB 28 1.2 1QQK-A nrPDB 26 3.0 5UBV-A nrPDB 26 4.2 4DPQ-A nrPDB 26 4.4 2AMX-A nrPDB 26 5.0 2OO3-A nrPDB 25 5.9 5EVJ-A nrPDB 25 6.0 5VK2-A nrPDB 25 8.0 5YHK-A nrPDB 25 8.9 CONVERGED! >1HCE-A nrPDB Length = 118 Score = 140 bits (352), Expect = 2e-34, Method: Composition-based stats. Identities = 118/118 (100%), Positives = 118/118 (100%) Query: 1 MGNRAFKSHHGHFLSAEGEAVKTHHGHHDHHTHFHVENHGGKVALKTHCGKYLSIGDHKQ 60 MGNRAFKSHHGHFLSAEGEAVKTHHGHHDHHTHFHVENHGGKVALKTHCGKYLSIGDHKQ Sbjct: 1 MGNRAFKSHHGHFLSAEGEAVKTHHGHHDHHTHFHVENHGGKVALKTHCGKYLSIGDHKQ 60 Query: 61 VYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHYISADHHGHVSTKEHHDHDTTFEEIII 118 VYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHYISADHHGHVSTKEHHDHDTTFEEIII Sbjct: 61 VYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHYISADHHGHVSTKEHHDHDTTFEEIII 118 >3P53-A nrPDB Length = 496 Score = 30.0 bits (66), Expect = 0.24, Method: Composition-based stats. Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 3/72 (4%) Query: 5 AFKSHHGHF--LSAEGEAVKTHHGHHDHHTHFHVENHGGKVALKTHCGKYLSIGDHKQVY 62 AF++H G + L+A G V++ + +F +E ++ L+ GK+++ + Q+ Sbjct: 309 AFRTHTGKYWTLTATG-GVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQLA 367 Query: 63 LSHHLHGDHSLF 74 S GD LF Sbjct: 368 ASVETAGDSELF 379 Score = 25.0 bits (53), Expect = 8.7, Method: Composition-based stats. Identities = 11/70 (15%), Positives = 31/70 (44%) Query: 33 HFHVENHGGKVALKTHCGKYLSIGDHKQVYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHY 92 ++ K A +TH GKY ++ V + F +E +++++ + + Sbjct: 298 QLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKF 357 Query: 93 ISADHHGHVS 102 +++ +G ++ Sbjct: 358 VTSKKNGQLA 367 >4HWV-A nrPDB Length = 503 Score = 29.2 bits (64), Expect = 0.43, Method: Composition-based stats. Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 10/56 (17%) Query: 58 HKQVYLSHHLHGDHSLFHLEHHGGKVSIKGHHHHYISADHHGHVSTKEHHDHDTTF 113 H+ V+ + + GD F ++HH G V + H ++ +++H HDHD F Sbjct: 259 HETVHGTDYSGGD---FKVQHHDGLVDVTRHGNYVTLSNNH-------FHDHDKAF 304 >6B02-A nrPDB Length = 341 Score = 27.7 bits (60), Expect = 1.2, Method: Composition-based stats. Identities = 18/63 (28%), Positives = 27/63 (42%), Gaps = 1/63 (1%) Query: 31 HTHFHVEN-HGGKVALKTHCGKYLSIGDHKQVYLSHHLHGDHSLFHLEHHGGKVSIKGHH 89 H HF + + V L + SIG H V + D+SLF +E + V K + Sbjct: 132 HAHFADKKIYTNLVELFNESLMHTSIGQHLDVTMERRQKSDYSLFTIERYNAIVKYKTAY 191 Query: 90 HHY 92 + Y Sbjct: 192 YTY 194 >1QQK-A nrPDB Length = 140 Score = 26.2 bits (56), Expect = 3.0, Method: Composition-based stats. Identities = 16/69 (23%), Positives = 34/69 (49%), Gaps = 2/69 (2%) Query: 52 YLSIGDHKQVYLSHHLHGDHSLFHLEHHG-GKVSIKG-HHHHYISADHHGHVSTKEHHDH 109 YL I +V + + +++ + G V+IKG +Y++ + G + K+ + Sbjct: 22 YLRIDKRGKVKGTQEMRNSYNIMEIRTVAVGIVAIKGVESEYYLAMNKEGKLYAKKECNE 81 Query: 110 DTTFEEIII 118 D F+E+I+ Sbjct: 82 DCNFKELIL 90 >5UBV-A nrPDB Length = 246 Score = 25.8 bits (55), Expect = 4.2, Method: Composition-based stats. Identities = 11/34 (32%), Positives = 17/34 (50%) Query: 23 THHGHHDHHTHFHVENHGGKVALKTHCGKYLSIG 56 T G D H H + + G++A+ H K + IG Sbjct: 159 TRPGRFDRHVHVSLPDVRGRIAILKHHAKKIKIG 192 >4DPQ-A nrPDB Length = 360 Score = 25.8 bits (55), Expect = 4.4, Method: Composition-based stats. Identities = 12/31 (38%), Positives = 17/31 (54%) Query: 69 GDHSLFHLEHHGGKVSIKGHHHHYISADHHG 99 G H+ H+ + GK SI+G H+ S H G Sbjct: 154 GMHAALHINPYYGKTSIEGLIAHFQSVLHMG 184 >2AMX-A nrPDB Length = 376 Score = 25.8 bits (55), Expect = 5.0, Method: Composition-based stats. Identities = 16/45 (35%), Positives = 20/45 (44%), Gaps = 8/45 (17%) Query: 39 HGGKVALK--------THCGKYLSIGDHKQVYLSHHLHGDHSLFH 75 H G A+K H G+ + + DHK VY S HG H H Sbjct: 195 HSGDFAIKHKHDFVGFDHGGREIDLKDHKDVYHSVRDHGLHLTVH 239 >2OO3-A nrPDB Length = 283 Score = 25.4 bits (54), Expect = 5.9, Method: Composition-based stats. Identities = 12/37 (32%), Positives = 19/37 (51%), Gaps = 2/37 (5%) Query: 48 HCGKYLSIGDHKQV--YLSHHLHGDHSLFHLEHHGGK 82 H G + + H + L++ H D LF+LE H G+ Sbjct: 10 HAGNFADVIKHITLTRLLAYLTHKDKPLFYLETHSGR 46 >5EVJ-A nrPDB Length = 387 Score = 25.4 bits (54), Expect = 6.0, Method: Composition-based stats. Identities = 11/23 (47%), Positives = 13/23 (56%) Query: 82 KVSIKGHHHHYISADHHGHVSTK 104 K +I GH H Y DHH V+ K Sbjct: 294 KGTIPGHSHAYDLDDHHRFVTNK 316 >5VK2-A nrPDB Length = 259 Score = 25.0 bits (53), Expect = 8.0, Method: Composition-based stats. Identities = 18/67 (26%), Positives = 29/67 (43%), Gaps = 14/67 (20%) Query: 51 KYLSIGD-HKQVYLSHHLHGDHSLFHL-------------EHHGGKVSIKGHHHHYISAD 96 K+ ++ D HK+ H L S FHL + +GGK+S++ + H + D Sbjct: 116 KFCNLSDAHKKNLYDHALMSIISTFHLSIPNFNQYEAMSCDFNGGKISVQYNLSHSYAGD 175 Query: 97 HHGHVST 103 H T Sbjct: 176 AANHCGT 182 >5YHK-A nrPDB Length = 238 Score = 24.6 bits (52), Expect = 8.9, Method: Composition-based stats. Identities = 13/45 (28%), Positives = 21/45 (46%), Gaps = 2/45 (4%) Query: 74 FHLEHHGGKVSIKGHHHHYISADHH--GHVSTKEHHDHDTTFEEI 116 F H +++ G+H H+I+ D GH+ + TF EI Sbjct: 162 FRTPQHMQGINVAGYHEHFITDDRQGGGHLLDYQLESGVLTFGEI 206 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.136 0.453 Lambda K H 0.267 0.0443 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,819,110 Number of Sequences: 36641 Number of extensions: 525119 Number of successful extensions: 8588 Number of sequences better than 10.0: 262 Number of HSP's better than 10.0 without gapping: 448 Number of HSP's successfully gapped in prelim test: 116 Number of HSP's that attempted gapping in prelim test: 5810 Number of HSP's gapped (non-prelim): 2211 length of query: 118 length of database: 10,336,785 effective HSP length: 83 effective length of query: 35 effective length of database: 7,295,582 effective search space: 255345370 effective search space used: 255345370 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 53 (24.9 bits)