BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P11551 (438 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3O7P-A nrPDB 883 0.0 1DCE-A nrPDB 31 1.2 5VBL-B nrPDB 29 4.5 >3O7P-A nrPDB Length = 438 Score = 883 bits (2281), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/438 (99%), Positives = 437/438 (99%) Query: 1 MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNLNDILLPQFQQAFTLTN 60 MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNLNDILLPQFQQAFTLTN Sbjct: 1 MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNLNDILLPQFQQAFTLTN 60 Query: 61 FQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYALGAALFWPAAEIMNYTLF 120 FQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYALGAALFWPAAEIMNYTLF Sbjct: 61 FQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYALGAALFWPAAEIMNYTLF 120 Query: 121 LVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSN 180 LVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQTF SFGAIIAVVFGQSLILSN Sbjct: 121 LVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQTFASFGAIIAVVFGQSLILSN 180 Query: 181 VPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHS 240 VPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHS Sbjct: 181 VPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHS 240 Query: 241 DAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANY 300 DAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANY Sbjct: 241 DAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANY 300 Query: 301 LTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAF 360 LTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAF Sbjct: 301 LTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAF 360 Query: 361 MSIQYPTIFSLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPAL 420 MSIQYPTIFSLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPAL Sbjct: 361 MSIQYPTIFSLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPAL 420 Query: 421 CFAVIFIFARFRSQTATN 438 CFAVIFIFARFRSQTATN Sbjct: 421 CFAVIFIFARFRSQTATN 438 >1DCE-A nrPDB Length = 567 Score = 30.8 bits (68), Expect = 1.2, Method: Compositional matrix adjust. Identities = 17/60 (28%), Positives = 27/60 (45%), Gaps = 5/60 (8%) Query: 313 FTGTWLISRFAPHKVLAAYALIAMALCLISAFA-----GGHVGLIALTLCSAFMSIQYPT 367 F WL+ R PH VL + CL F+ G +G + L + A +S+++ T Sbjct: 230 FYHRWLLGRAEPHDVLCCVHVSREEACLSVCFSRPLTVGSRMGTLLLMVDEAPLSVEWRT 289 >5VBL-B nrPDB Length = 407 Score = 28.9 bits (63), Expect = 4.5, Method: Compositional matrix adjust. Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 4/43 (9%) Query: 100 LYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFV 142 LY LG+ L WP ++ LFL+ +F + + + NPF+ Sbjct: 329 LYMLGSLLHWPC----DFDLFLMNIFPYCTCISYVNSCLNPFL 367 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3O7P-A nrPDB 778 0.0 Sequences not found previously or not previously below threshold: 2JO1-A nrPDB 31 0.72 CONVERGED! >3O7P-A nrPDB Length = 438 Score = 778 bits (2010), Expect = 0.0, Method: Composition-based stats. Identities = 437/438 (99%), Positives = 437/438 (99%) Query: 1 MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNLNDILLPQFQQAFTLTN 60 MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNLNDILLPQFQQAFTLTN Sbjct: 1 MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNLNDILLPQFQQAFTLTN 60 Query: 61 FQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYALGAALFWPAAEIMNYTLF 120 FQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYALGAALFWPAAEIMNYTLF Sbjct: 61 FQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYALGAALFWPAAEIMNYTLF 120 Query: 121 LVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSN 180 LVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQTF SFGAIIAVVFGQSLILSN Sbjct: 121 LVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQTFASFGAIIAVVFGQSLILSN 180 Query: 181 VPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHS 240 VPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHS Sbjct: 181 VPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHS 240 Query: 241 DAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANY 300 DAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANY Sbjct: 241 DAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANY 300 Query: 301 LTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAF 360 LTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAF Sbjct: 301 LTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAF 360 Query: 361 MSIQYPTIFSLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPAL 420 MSIQYPTIFSLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPAL Sbjct: 361 MSIQYPTIFSLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPAL 420 Query: 421 CFAVIFIFARFRSQTATN 438 CFAVIFIFARFRSQTATN Sbjct: 421 CFAVIFIFARFRSQTATN 438 >2JO1-A nrPDB Length = 72 Score = 31.4 bits (70), Expect = 0.72, Method: Composition-based stats. Identities = 17/57 (29%), Positives = 32/57 (56%), Gaps = 5/57 (8%) Query: 207 SVQTPYMIIVAIVLLVALLIMLT-----KFPALQSDNHSDAKQGSFSASLSRLARIR 258 S+Q ++I I+ ++ +LI+L+ KF Q D ++G+F +S+ RL+ R Sbjct: 15 SLQIGGLVIAGILFILGILIVLSRRCRCKFNQQQRTGEPDEEEGTFRSSIRRLSTRR 71 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.323 0.146 0.470 Lambda K H 0.267 0.0460 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 20,173,647 Number of Sequences: 36641 Number of extensions: 961282 Number of successful extensions: 2187 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 18 Number of HSP's that attempted gapping in prelim test: 2174 Number of HSP's gapped (non-prelim): 26 length of query: 438 length of database: 10,336,785 effective HSP length: 100 effective length of query: 338 effective length of database: 6,672,685 effective search space: 2255367530 effective search space used: 2255367530 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.4 bits) S2: 61 (27.9 bits)