BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P10746 (265 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1JR2-A nrPDB 545 e-156 3RE1-A nrPDB 45 4e-05 3MW8-A nrPDB 31 0.63 6GZH-B nrPDB 28 3.6 4BN9-A nrPDB 28 3.7 5WUG-A nrPDB 27 9.0 4XGV-A nrPDB 27 9.0 5IV7-F nrPDB 27 9.5 >1JR2-A nrPDB Length = 286 Score = 545 bits (1404), Expect = e-156, Method: Compositional matrix adjust. Identities = 265/265 (100%), Positives = 265/265 (100%) Query: 1 MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLI 60 MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLI Sbjct: 22 MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLI 81 Query: 61 FTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCG 120 FTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCG Sbjct: 82 FTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCG 141 Query: 121 NAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNL 180 NAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNL Sbjct: 142 NAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNL 201 Query: 181 NSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVS 240 NSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVS Sbjct: 202 NSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVS 261 Query: 241 CTAESPTPQALATGIRKALQPHGCC 265 CTAESPTPQALATGIRKALQPHGCC Sbjct: 262 CTAESPTPQALATGIRKALQPHGCC 286 >3RE1-A nrPDB Length = 269 Score = 44.7 bits (104), Expect = 4e-05, Method: Compositional matrix adjust. Identities = 45/173 (26%), Positives = 86/173 (49%), Gaps = 19/173 (10%) Query: 2 KVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSL-PSFSEKLSHPEDYGGLI 60 ++LL + A+E R L G+ ++ +P+L E L L P+ + +Y +I Sbjct: 16 RLLLTRPAEESAA----LARVLADAGIFSSSLPLLETEPLPLTPAQRSIIFELLNYSAVI 71 Query: 61 FTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCG 120 S AA L +E + EVW + + W + VG+AT ++ GLD G Sbjct: 72 VVS---KPAARLAIELID--EVWPQPPMQPW-----FSVGSATGQILLDYGLDASWPEQG 121 Query: 121 N-AEKLAEYICSRESSALP---LLFPCGNLKREILPKALKDKGIAMESITVYQ 169 + +E L ++ +++ A+P +L GN RE+L + L+++G+ ++ + +Y+ Sbjct: 122 DDSEALLDHPRLKQAIAVPGSRVLIMRGNEGRELLAEQLRERGVGVDYLPLYR 174 >3MW8-A nrPDB Length = 240 Score = 30.8 bits (68), Expect = 0.63, Method: Compositional matrix adjust. Identities = 23/90 (25%), Positives = 39/90 (43%), Gaps = 2/90 (2%) Query: 87 LKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGN--AEKLAEYICSRESSALPLLFPCG 144 LK++W + Y VG+ATA ++ G+ E + E L + S ++ G Sbjct: 69 LKDQWPKATYYAVGDATADALALQGITAERSPADSQATEGLLTLPSLEQVSGKQIVIVRG 128 Query: 145 NLKREILPKALKDKGIAMESITVYQTVAHP 174 RE + L+ +G + + VYQ P Sbjct: 129 KGGREAMADGLRLRGANVSYLEVYQRACPP 158 >6GZH-B nrPDB Length = 726 Score = 28.1 bits (61), Expect = 3.6, Method: Compositional matrix adjust. Identities = 12/29 (41%), Positives = 16/29 (55%) Query: 36 LSFEFLSLPSFSEKLSHPEDYGGLIFTSP 64 L+F F SLP+ + H D GL F+ P Sbjct: 600 LNFSFPSLPTMGQMPGHSSDTSGLSFSQP 628 >4BN9-A nrPDB Length = 201 Score = 28.1 bits (61), Expect = 3.7, Method: Compositional matrix adjust. Identities = 14/46 (30%), Positives = 22/46 (47%) Query: 196 FSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVSC 241 FSP+ + L+GD D++ + P A+ AQG+P S Sbjct: 35 FSPTAFNSQTGRLLILTGDAQDKLWDEIVAPELKAAMEAQGVPESA 80 >5WUG-A nrPDB Length = 955 Score = 26.9 bits (58), Expect = 9.0, Method: Compositional matrix adjust. Identities = 13/44 (29%), Positives = 22/44 (50%), Gaps = 4/44 (9%) Query: 221 FAAIGPTTARALAAQGLPVSCTAESPTPQALATGIRKALQPHGC 264 F +G L G+PV+CTA+ P+ + +G++ P G Sbjct: 551 FGGVGEN----LLEYGIPVACTADGPSGIRMDSGLKATQLPIGT 590 >4XGV-A nrPDB Length = 340 Score = 26.9 bits (58), Expect = 9.0, Method: Compositional matrix adjust. Identities = 19/83 (22%), Positives = 32/83 (38%), Gaps = 1/83 (1%) Query: 184 YSQQGVPASITFFSPSGLTYSLKHIQEL-SGDNIDQIKFAAIGPTTARALAAQGLPVSCT 242 Y Q+ +P S G Y+ H+ L + I + + G +R + +GLP Sbjct: 152 YLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGLPWRVA 211 Query: 243 AESPTPQALATGIRKALQPHGCC 265 + PT + A + HG Sbjct: 212 IQKPTDKENAVQAVVDINGHGIS 234 >5IV7-F nrPDB Length = 288 Score = 26.9 bits (58), Expect = 9.5, Method: Compositional matrix adjust. Identities = 12/38 (31%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Query: 57 GGLIFTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAK 94 G L+ TSP + + C+ +N T VW S++ + K Sbjct: 135 GNLVVTSPYS-KVTLRCISSDNSTSVWNYSIESMFGQK 171 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1JR2-A nrPDB 498 e-141 3RE1-A nrPDB 213 5e-56 Sequences not found previously or not previously below threshold: 4ES6-A nrPDB 129 8e-31 3MW8-A nrPDB 63 1e-10 3D8N-A nrPDB 32 0.20 4BN9-A nrPDB 30 1.4 1QVR-A nrPDB 30 1.4 2EKL-A nrPDB 28 3.8 >1JR2-A nrPDB Length = 286 Score = 498 bits (1281), Expect = e-141, Method: Composition-based stats. Identities = 265/265 (100%), Positives = 265/265 (100%) Query: 1 MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLI 60 MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLI Sbjct: 22 MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLI 81 Query: 61 FTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCG 120 FTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCG Sbjct: 82 FTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCG 141 Query: 121 NAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNL 180 NAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNL Sbjct: 142 NAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNL 201 Query: 181 NSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVS 240 NSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVS Sbjct: 202 NSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVS 261 Query: 241 CTAESPTPQALATGIRKALQPHGCC 265 CTAESPTPQALATGIRKALQPHGCC Sbjct: 262 CTAESPTPQALATGIRKALQPHGCC 286 >3RE1-A nrPDB Length = 269 Score = 213 bits (543), Expect = 5e-56, Method: Composition-based stats. Identities = 45/173 (26%), Positives = 86/173 (49%), Gaps = 19/173 (10%) Query: 2 KVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSL-PSFSEKLSHPEDYGGLI 60 ++LL + A+E R L G+ ++ +P+L E L L P+ + +Y +I Sbjct: 16 RLLLTRPAEESAA----LARVLADAGIFSSSLPLLETEPLPLTPAQRSIIFELLNYSAVI 71 Query: 61 FTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCG 120 S AA L +E + EVW + + W + VG+AT ++ GLD G Sbjct: 72 VVS---KPAARLAIELID--EVWPQPPMQPW-----FSVGSATGQILLDYGLDASWPEQG 121 Query: 121 N-AEKLAEYICSRESSALP---LLFPCGNLKREILPKALKDKGIAMESITVYQ 169 + +E L ++ +++ A+P +L GN RE+L + L+++G+ ++ + +Y+ Sbjct: 122 DDSEALLDHPRLKQAIAVPGSRVLIMRGNEGRELLAEQLRERGVGVDYLPLYR 174 >4ES6-A nrPDB Length = 254 Score = 129 bits (325), Expect = 8e-31, Method: Composition-based stats. Identities = 40/176 (22%), Positives = 79/176 (44%), Gaps = 19/176 (10%) Query: 2 KVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLS-LPSFSEKLSHPEDYGGLI 60 ++LL + +E LG G+ ++ +P+L+ + L P + + Y ++ Sbjct: 8 RLLLTRPDEECAA----LAASLGEAGVHSSSLPLLAIDPLEETPEQRTLMLDLDRYCAVV 63 Query: 61 FTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCG 120 S AA L LE+ ++ W + ++ W VG ATA+++ GLD G Sbjct: 64 VVS---KPAARLGLERLDRY--WPQPPQQTWC-----SVGAATAAILEAYGLDVTYPEQG 113 Query: 121 N-AEKLAEYICSRESSAL---PLLFPCGNLKREILPKALKDKGIAMESITVYQTVA 172 + +E L ++S + +L G RE L + L+ +G+ ++ + +Y+ A Sbjct: 114 DDSEALLALPAFQDSLRVHDPKVLIMRGEGGREFLAERLRGQGVQVDYLPLYRRRA 169 >3MW8-A nrPDB Length = 240 Score = 63.2 bits (152), Expect = 1e-10, Method: Composition-based stats. Identities = 42/176 (23%), Positives = 67/176 (38%), Gaps = 21/176 (11%) Query: 1 MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLI 60 MK+LL + ++ Y +E P+LS E ++ +L LI Sbjct: 2 MKLLLTRPEGKNAAMASALDALAIPYLVE----PLLSVEAAAVT--QAQLDELSRADILI 55 Query: 61 FTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCG 120 F S AV A W LK++W + Y VG+ATA ++ G+ E Sbjct: 56 FISTSAVSFA----------TPW---LKDQWPKATYYAVGDATADALALQGITAERSPAD 102 Query: 121 N--AEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHP 174 + E L + S ++ G RE + L+ +G + + VYQ P Sbjct: 103 SQATEGLLTLPSLEQVSGKQIVIVRGKGGREAMADGLRLRGANVSYLEVYQRACPP 158 >3D8N-A nrPDB Length = 286 Score = 32.4 bits (72), Expect = 0.20, Method: Composition-based stats. Identities = 44/246 (17%), Positives = 84/246 (34%), Gaps = 30/246 (12%) Query: 27 GLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLIFTSPRAVEAAELCLEQNNKTEVWERS 86 G L PV + E + +P + +++ G +F + V +L + Sbjct: 55 GFTPLLFPVQATEKVPVPEYRDQVRELAQ-GVDLFLATTGVGVRDL----LEAGKALGLD 109 Query: 87 LKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGNAEKLAEYICSRESSALPLLFPCGNL 146 L+ G A + + GL G ++ L + + L G Sbjct: 110 LEGPLAKAFRLARGAKAARALKEAGLPPHAVGDGTSKSLL--PLLPQGRGVAALQLYGK- 166 Query: 147 KREILPKALKDKGIAMESITVYQTVAHPG---------IQGNLNSYYSQQGVPASITF-- 195 +L AL ++G + + Y+ + P ++G +++ + F Sbjct: 167 PLPLLENALAERGYRVLPLMPYRHLPDPEGILRLEEAVLRGEVDALAFVAAIQVEFLFEG 226 Query: 196 -FSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVSCTAESPTPQALATG 254 P L +L ++K A+G TA AL G+ E+ +L G Sbjct: 227 AKDPKALREALN----------TRVKALAVGRVTADALREWGVKPFYVDETERLGSLLQG 276 Query: 255 IRKALQ 260 ++ALQ Sbjct: 277 FKRALQ 282 >4BN9-A nrPDB Length = 201 Score = 29.7 bits (65), Expect = 1.4, Method: Composition-based stats. Identities = 14/45 (31%), Positives = 22/45 (48%) Query: 196 FSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVS 240 FSP+ + L+GD D++ + P A+ AQG+P S Sbjct: 35 FSPTAFNSQTGRLLILTGDAQDKLWDEIVAPELKAAMEAQGVPES 79 >1QVR-A nrPDB Length = 854 Score = 29.7 bits (65), Expect = 1.4, Method: Composition-based stats. Identities = 20/77 (25%), Positives = 33/77 (42%), Gaps = 3/77 (3%) Query: 105 SLVSKIGLDTEGETCGNAEKLAEYICSRESSALPLLFPC--GNLKR-EILPKALKDKGIA 161 L+ K G D + +LA + L G L R E L + LKD+ +A Sbjct: 48 RLLEKAGADPKALKELQERELARLPKVEGAEVGQYLTSRLSGALNRAEGLMEELKDRYVA 107 Query: 162 MESITVYQTVAHPGIQG 178 ++++ + A PG+ G Sbjct: 108 VDTLVLALAEATPGLPG 124 >2EKL-A nrPDB Length = 313 Score = 28.2 bits (61), Expect = 3.8, Method: Composition-based stats. Identities = 8/18 (44%), Positives = 13/18 (72%) Query: 149 EILPKALKDKGIAMESIT 166 EIL K L++KGI ++ + Sbjct: 16 EILIKTLREKGIQVDYMP 33 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.311 0.129 0.356 Lambda K H 0.267 0.0398 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,958,430 Number of Sequences: 36641 Number of extensions: 385764 Number of successful extensions: 1105 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 1073 Number of HSP's gapped (non-prelim): 21 length of query: 265 length of database: 10,336,785 effective HSP length: 95 effective length of query: 170 effective length of database: 6,855,890 effective search space: 1165501300 effective search space used: 1165501300 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.3 bits) S2: 58 (27.0 bits)