BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P0C0S1 (286 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2OAU-A nrPDB 563 e-161 4HW9-A nrPDB 140 6e-34 3T9N-A nrPDB 80 9e-16 5Y4O-A nrPDB 65 2e-11 4H9D-A nrPDB 30 1.3 1ODL-A nrPDB 29 1.9 4O2Z-A nrPDB 29 2.5 >2OAU-A nrPDB Length = 306 Score = 563 bits (1451), Expect = e-161, Method: Compositional matrix adjust. Identities = 286/286 (100%), Positives = 286/286 (100%) Query: 1 MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLIIARMISNAVNRLMISRK 60 MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLIIARMISNAVNRLMISRK Sbjct: 21 MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLIIARMISNAVNRLMISRK 80 Query: 61 IDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAA 120 IDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAA Sbjct: 81 IDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAA 140 Query: 121 GVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSR 180 GVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSR Sbjct: 141 GVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSR 200 Query: 181 EPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVW 240 EPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVW Sbjct: 201 EPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVW 260 Query: 241 SNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVNFKRVKEDKAA 286 SNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVNFKRVKEDKAA Sbjct: 261 SNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVNFKRVKEDKAA 306 >4HW9-A nrPDB Length = 309 Score = 140 bits (353), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 85/254 (33%), Positives = 154/254 (60%), Gaps = 3/254 (1%) Query: 26 SYAVNIVAALAIIIVGLIIARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAA 85 + + ++ A+ + +G + + N +L+ K D +A+F++ + I+ T I A Sbjct: 41 HFGIILIKAVIVFCIGFYFSFFLRNKTMKLL--SKKDEILANFVAQVTFILILIITTIIA 98 Query: 86 LGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTV 145 L +GVQT S+I VLG G+AV LAL+ LS++A G++L++ PF+ G+ +++ G+ G V Sbjct: 99 LSTLGVQTTSIITVLGTVGIAVALALKDYLSSIAGGIILIILHPFKKGDIIEISGLEGKV 158 Query: 146 LSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQIL 205 ++ F+T++R DG++ V+PN + NIIN + RR E++ GV Y SDI+ V + + Sbjct: 159 EALNFFNTSLRLHDGRLAVLPNRSVANSNIINSNNTACRRIEWVCGVGYGSDIELVHKTI 218 Query: 206 TNIIQSEDRILKDREMTVRLNELGASSINFVVRVWSNSGD-LQNVYWDVLERIKREFDAA 264 ++I + ++I K+ + + + G+SS+NF +RVW+ D + NV +++ERIK DA Sbjct: 219 KDVIDTMEKIDKNMPTFIGITDFGSSSLNFTIRVWAKIEDGIFNVRSELIERIKNALDAN 278 Query: 265 GISFPYPQMDVNFK 278 I P+ ++D+ K Sbjct: 279 HIEIPFNKLDIAIK 292 >3T9N-A nrPDB Length = 282 Score = 80.5 bits (197), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 60/226 (26%), Positives = 109/226 (48%), Gaps = 17/226 (7%) Query: 58 SRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSN 117 RKID T+ VRY I + L + S++AV G LA+G Q + + Sbjct: 58 QRKID-TLTSLTKNAVRYIIYFLAGASILKLFNIDMTSLLAVAGIGSLAIGFGAQNLVKD 116 Query: 118 LAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADGKIIVIPNGKIIAGNIIN 177 + +G ++ F G+YV + G++GTV + + T +R + +IPNG+I + N Sbjct: 117 MISGFFIIFEDQFSVGDYVTINGISGTVEEIGLRVTKIRGFSDGLHIIPNGEI--KMVTN 174 Query: 178 FSREPVRRNEFIIGVAY--DSDIDQV----KQILTNIIQSEDRILKDREMTVRLNELGAS 231 +++ + ++ +A+ D D+D++ ++I + +S D ++ E L Sbjct: 175 LTKDSMMA---VVNIAFPIDEDVDKIIEGLQEICEEVKKSRDDLI---EGPTVLGITDMQ 228 Query: 232 SINFVVRVWSNSGDLQN--VYWDVLERIKREFDAAGISFPYPQMDV 275 V+ V++ + +Q V D+ R+K+ FD ISFPYP+ V Sbjct: 229 DSKLVIMVYAKTQPMQKWAVERDIRYRVKKMFDQKNISFPYPRTTV 274 >5Y4O-A nrPDB Length = 349 Score = 65.5 bits (158), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 53/236 (22%), Positives = 112/236 (47%), Gaps = 15/236 (6%) Query: 47 MISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGAAGLA 106 ++ N + I + D T A +S +++ II ++ G+ + ++ G GLA Sbjct: 96 LLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLA 155 Query: 107 VGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLG--GVAGTVLSVQIFSTTMRTADGKIIV 164 VG+A + LSN +G++L RPF G+++ + GTV + T + T D + + Sbjct: 156 VGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLY 215 Query: 165 IPNGKIIAGNIINFSREPVRRNEFIIGVAYD--SDIDQVKQILTNIIQSEDRILKDREMT 222 +PN + ++ N R RR IG+ Y+ + + + + + ++++ I + + + Sbjct: 216 VPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLL 275 Query: 223 VRLNELGASSINFVVRVWSNS-------GDLQNVYWDVLERIKREFDAAGISFPYP 271 V N+ SS+N +V ++ + Q+VY +++ ++ + G F +P Sbjct: 276 VYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQ----SHGADFAFP 327 >4H9D-A nrPDB Length = 112 Score = 30.0 bits (66), Expect = 1.3, Method: Compositional matrix adjust. Identities = 13/54 (24%), Positives = 28/54 (51%) Query: 149 QIFSTTMRTADGKIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVK 202 +IF T D + V+ GK GN++ +E R ++++ V ++ +D ++ Sbjct: 43 EIFPPEELTMDHLVPVVRGGKSTRGNVVPACKECNNRKKYLLPVEWEEYLDSLE 96 >1ODL-A nrPDB Length = 235 Score = 29.3 bits (64), Expect = 1.9, Method: Compositional matrix adjust. Identities = 17/42 (40%), Positives = 27/42 (64%), Gaps = 5/42 (11%) Query: 63 ATVADFLSALVRYGIIAFTLIAA----LGRV-GVQTASVIAV 99 AT + A RYG++AF + A+ LGR+ GV+T +++AV Sbjct: 161 ATTPEEARAWARYGVLAFEMEASALFLLGRMRGVRTGAILAV 202 >4O2Z-A nrPDB Length = 377 Score = 28.9 bits (63), Expect = 2.5, Method: Compositional matrix adjust. Identities = 22/78 (28%), Positives = 38/78 (48%), Gaps = 6/78 (7%) Query: 198 IDQVKQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVWSNSGDLQNV-----YWD 252 +DQ++ I +I + I++ RE +LNEL S+N V +G+ + + W Sbjct: 257 MDQLRMIAQHIGKPPASIVEHREALEKLNELPDGSLNIPKLVPGLAGNTEGIDFLSKMW- 315 Query: 253 VLERIKREFDAAGISFPY 270 L+ KR A ++ PY Sbjct: 316 TLDPSKRPTAADMLAHPY 333 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2OAU-A nrPDB 364 e-101 4HW9-A nrPDB 264 3e-71 5Y4O-A nrPDB 251 3e-67 3T9N-A nrPDB 221 2e-58 Sequences not found previously or not previously below threshold: 3Q1N-A nrPDB 32 0.37 CONVERGED! >2OAU-A nrPDB Length = 306 Score = 364 bits (935), Expect = e-101, Method: Composition-based stats. Identities = 286/286 (100%), Positives = 286/286 (100%) Query: 1 MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLIIARMISNAVNRLMISRK 60 MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLIIARMISNAVNRLMISRK Sbjct: 21 MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLIIARMISNAVNRLMISRK 80 Query: 61 IDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAA 120 IDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAA Sbjct: 81 IDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAA 140 Query: 121 GVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSR 180 GVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSR Sbjct: 141 GVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSR 200 Query: 181 EPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVW 240 EPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVW Sbjct: 201 EPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVW 260 Query: 241 SNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVNFKRVKEDKAA 286 SNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVNFKRVKEDKAA Sbjct: 261 SNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVNFKRVKEDKAA 306 >4HW9-A nrPDB Length = 309 Score = 264 bits (675), Expect = 3e-71, Method: Composition-based stats. Identities = 85/254 (33%), Positives = 154/254 (60%), Gaps = 3/254 (1%) Query: 26 SYAVNIVAALAIIIVGLIIARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAA 85 + + ++ A+ + +G + + N +L+ K D +A+F++ + I+ T I A Sbjct: 41 HFGIILIKAVIVFCIGFYFSFFLRNKTMKLL--SKKDEILANFVAQVTFILILIITTIIA 98 Query: 86 LGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTV 145 L +GVQT S+I VLG G+AV LAL+ LS++A G++L++ PF+ G+ +++ G+ G V Sbjct: 99 LSTLGVQTTSIITVLGTVGIAVALALKDYLSSIAGGIILIILHPFKKGDIIEISGLEGKV 158 Query: 146 LSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQIL 205 ++ F+T++R DG++ V+PN + NIIN + RR E++ GV Y SDI+ V + + Sbjct: 159 EALNFFNTSLRLHDGRLAVLPNRSVANSNIINSNNTACRRIEWVCGVGYGSDIELVHKTI 218 Query: 206 TNIIQSEDRILKDREMTVRLNELGASSINFVVRVWSNSGD-LQNVYWDVLERIKREFDAA 264 ++I + ++I K+ + + + G+SS+NF +RVW+ D + NV +++ERIK DA Sbjct: 219 KDVIDTMEKIDKNMPTFIGITDFGSSSLNFTIRVWAKIEDGIFNVRSELIERIKNALDAN 278 Query: 265 GISFPYPQMDVNFK 278 I P+ ++D+ K Sbjct: 279 HIEIPFNKLDIAIK 292 >5Y4O-A nrPDB Length = 349 Score = 251 bits (641), Expect = 3e-67, Method: Composition-based stats. Identities = 55/263 (20%), Positives = 121/263 (46%), Gaps = 15/263 (5%) Query: 29 VNIVAALAIIIVGLIIARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGR 88 ++ + I ++ I ++ N + I + D T A +S +++ II ++ Sbjct: 78 IDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEH 137 Query: 89 VGVQTASVIAVLGAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLG--GVAGTVL 146 G+ + ++ G GLAVG+A + LSN +G++L RPF G+++ + GTV Sbjct: 138 FGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVA 197 Query: 147 SVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYD--SDIDQVKQI 204 + T + T D + + +PN + ++ N R RR IG+ Y+ + + + + Sbjct: 198 EIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEA 257 Query: 205 LTNIIQSEDRILKDREMTVRLNELGASSINFVVRVWSNS-------GDLQNVYWDVLERI 257 + ++++ I + + + V N+ SS+N +V ++ + Q+VY +++ + Sbjct: 258 VREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIV 317 Query: 258 KREFDAAGISFPYPQMDVNFKRV 280 + + G F +P + + Sbjct: 318 Q----SHGADFAFPSQTLYMDNI 336 >3T9N-A nrPDB Length = 282 Score = 221 bits (564), Expect = 2e-58, Method: Composition-based stats. Identities = 64/273 (23%), Positives = 124/273 (45%), Gaps = 22/273 (8%) Query: 25 LSYAVNIVAALAIIIVGLIIARMISNAVNRLMISRKIDA------TVADFLSALVRYGII 78 + + ++++ L I +G+ A + +L KI T+ VRY I Sbjct: 18 VKFLLDVLKILIIAFIGIKFADFLIYRFYKLYSKSKIQLPQRKIDTLTSLTKNAVRYIIY 77 Query: 79 AFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDL 138 + L + S++AV G LA+G Q + ++ +G ++ F G+YV + Sbjct: 78 FLAGASILKLFNIDMTSLLAVAGIGSLAIGFGAQNLVKDMISGFFIIFEDQFSVGDYVTI 137 Query: 139 GGVAGTVLSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAY--DS 196 G++GTV + + T +R + +IPNG+I + N +++ + ++ +A+ D Sbjct: 138 NGISGTVEEIGLRVTKIRGFSDGLHIIPNGEI--KMVTNLTKDSMMA---VVNIAFPIDE 192 Query: 197 DIDQV----KQILTNIIQSEDRILKDREMTVRLNELGASSINFVVRVWSNSGDLQN--VY 250 D+D++ ++I + +S D ++ E L V+ V++ + +Q V Sbjct: 193 DVDKIIEGLQEICEEVKKSRDDLI---EGPTVLGITDMQDSKLVIMVYAKTQPMQKWAVE 249 Query: 251 WDVLERIKREFDAAGISFPYPQMDVNFKRVKED 283 D+ R+K+ FD ISFPYP+ V K + Sbjct: 250 RDIRYRVKKMFDQKNISFPYPRTTVILSEKKTN 282 >3Q1N-A nrPDB Length = 294 Score = 31.6 bits (70), Expect = 0.37, Method: Composition-based stats. Identities = 13/53 (24%), Positives = 24/53 (45%), Gaps = 5/53 (9%) Query: 129 PFRAGEYVDLGGVAGTVLSVQIFSTTMRTADGKIIVIPNGKIIAGNIINFSRE 181 PFR G+ D+ G G L + T DG ++++ N +I + + + Sbjct: 179 PFRNGDVEDIPGAEGNRLPL-----THDLLDGGLVILANSEIAKATLASPHHD 226 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.135 0.322 Lambda K H 0.267 0.0417 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,780,957 Number of Sequences: 36641 Number of extensions: 298803 Number of successful extensions: 1066 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 23 Number of HSP's that attempted gapping in prelim test: 1041 Number of HSP's gapped (non-prelim): 36 length of query: 286 length of database: 10,336,785 effective HSP length: 96 effective length of query: 190 effective length of database: 6,819,249 effective search space: 1295657310 effective search space used: 1295657310 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 59 (27.3 bits)