BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P0AGK4 (97 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1LN4-A nrPDB 187 6e-49 1JO0-A nrPDB 140 1e-34 1RQ8-A nrPDB 71 1e-13 3MKQ-A nrPDB 28 1.1 2YNP-A nrPDB 28 1.1 5VHO-A nrPDB 27 1.4 2XRX-B nrPDB 27 1.5 2CVO-A nrPDB 26 3.0 5VFU-A nrPDB 26 3.6 1POI-A nrPDB 25 5.1 6CFO-B nrPDB 25 5.3 3QZY-A nrPDB 25 6.3 5XTP-A nrPDB 25 6.7 3H4M-A nrPDB 25 6.9 6EPC-H nrPDB 25 7.5 5UJW-A nrPDB 25 8.6 >1LN4-A nrPDB Length = 104 Score = 187 bits (476), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 95/96 (98%), Positives = 96/96 (100%) Query: 1 MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKT 60 M+LSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKT Sbjct: 1 MDLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKT 60 Query: 61 LIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLP 96 LIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLP Sbjct: 61 LIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLP 96 >1JO0-A nrPDB Length = 98 Score = 140 bits (354), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 72/95 (75%), Positives = 77/95 (81%) Query: 3 LSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKTLI 62 LSTKQKQ LKGLAH L PVV+LG NGLTEGVLAEIE AL HHELIKVK+A DRETK LI Sbjct: 3 LSTKQKQFLKGLAHHLNPVVMLGGNGLTEGVLAEIENALNHHELIKVKVAGADRETKQLI 62 Query: 63 VEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR 97 + AIVRET A VQ IG LVLYRP++E KI LPR Sbjct: 63 INAIVRETKAAQVQTIGHILVLYRPSEEAKIQLPR 97 >1RQ8-A nrPDB Length = 104 Score = 71.2 bits (173), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 37/95 (38%), Positives = 59/95 (62%), Gaps = 1/95 (1%) Query: 3 LSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKTLI 62 L+ KQK++L+ LAH + P+ +G G+ E ++ +I+ LE+ ELIKV + + + K + Sbjct: 2 LTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRELIKVHVLQNNFDDKKEL 61 Query: 63 VEAIVRETGACNVQVIGKTLVLYRPTKERK-ISLP 96 E + T + VQVIG +V+YR +KE K I LP Sbjct: 62 AETLSEATRSELVQVIGSMIVIYRESKENKEIELP 96 >3MKQ-A nrPDB Length = 814 Score = 27.7 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 17/51 (33%), Positives = 24/51 (47%), Gaps = 5/51 (9%) Query: 16 HPLKPVVLLGSNGLTEGVLA-----EIEQALEHHELIKVKIATEDRETKTL 61 HP KP VL GS+ LT + +EQ E HE + +A ++ T Sbjct: 106 HPTKPYVLSGSDDLTVKLWNWENNWALEQTFEGHEHFVMCVAFNPKDPSTF 156 >2YNP-A nrPDB Length = 604 Score = 27.7 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 17/51 (33%), Positives = 24/51 (47%), Gaps = 5/51 (9%) Query: 16 HPLKPVVLLGSNGLTEGVLA-----EIEQALEHHELIKVKIATEDRETKTL 61 HP KP VL GS+ LT + +EQ E HE + +A ++ T Sbjct: 106 HPTKPYVLSGSDDLTVKLWNWENNWALEQTFEGHEHFVMCVAFNPKDPSTF 156 >5VHO-A nrPDB Length = 267 Score = 27.3 bits (59), Expect = 1.4, Method: Compositional matrix adjust. Identities = 12/25 (48%), Positives = 15/25 (60%) Query: 59 KTLIVEAIVRETGACNVQVIGKTLV 83 KTL A+ T AC ++VIG LV Sbjct: 65 KTLCARAVANRTDACFIRVIGSELV 89 >2XRX-B nrPDB Length = 188 Score = 27.3 bits (59), Expect = 1.5, Method: Compositional matrix adjust. Identities = 11/38 (28%), Positives = 20/38 (52%) Query: 58 TKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISL 95 T+ L+ IV+ET + + +LYR ER++ + Sbjct: 110 TRHLVSNVIVKETATPDTFEVNSAFILYRNRLERQVDI 147 >2CVO-A nrPDB Length = 366 Score = 26.2 bits (56), Expect = 3.0, Method: Compositional matrix adjust. Identities = 13/38 (34%), Positives = 23/38 (60%), Gaps = 3/38 (7%) Query: 21 VVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRET 58 + +LG++G T AEI + L +H ++K+ T DR+ Sbjct: 26 IAVLGASGYTG---AEIVRLLANHPQFRIKVMTADRKA 60 >5VFU-A nrPDB Length = 361 Score = 26.2 bits (56), Expect = 3.6, Method: Composition-based stats. Identities = 12/25 (48%), Positives = 15/25 (60%) Query: 59 KTLIVEAIVRETGACNVQVIGKTLV 83 KTL A+ T AC ++VIG LV Sbjct: 150 KTLCARAVANRTDACFIRVIGSELV 174 >1POI-A nrPDB Length = 317 Score = 25.4 bits (54), Expect = 5.1, Method: Composition-based stats. Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 2/35 (5%) Query: 10 HLKGLAHPLKPVVLLGSNGLTE--GVLAEIEQALE 42 H L P PV L+ +GLT+ G+ E+ + L+ Sbjct: 110 HAAALGLPFLPVTLMQGSGLTDEWGISKEVRKTLD 144 >6CFO-B nrPDB Length = 341 Score = 25.4 bits (54), Expect = 5.3, Method: Composition-based stats. Identities = 20/67 (29%), Positives = 29/67 (43%), Gaps = 5/67 (7%) Query: 4 STKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALE----HHELIKVKIATEDRETK 59 S + ++ G P+ P+V G NG + GV A+ Q H +KV + K Sbjct: 108 SAAKTYYMSGGLQPV-PIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAK 166 Query: 60 TLIVEAI 66 LI AI Sbjct: 167 GLIKSAI 173 >3QZY-A nrPDB Length = 260 Score = 25.4 bits (54), Expect = 6.3, Method: Compositional matrix adjust. Identities = 11/44 (25%), Positives = 23/44 (52%) Query: 41 LEHHELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVL 84 + H ELI++ + E T V++ +ET +V V+ +++ Sbjct: 170 IYHIELIEIALDKEKYGTDITFVDSYQQETAGADVAVVSNNMLM 213 >5XTP-A nrPDB Length = 293 Score = 25.0 bits (53), Expect = 6.7, Method: Composition-based stats. Identities = 11/42 (26%), Positives = 22/42 (52%) Query: 43 HHELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVL 84 H ELI++ + E T V++ +ET +V V+ +++ Sbjct: 206 HIELIEIALDKEKYGTDITFVDSYQQETAGADVAVVSNNMLM 247 >3H4M-A nrPDB Length = 285 Score = 25.0 bits (53), Expect = 6.9, Method: Compositional matrix adjust. Identities = 11/25 (44%), Positives = 17/25 (68%) Query: 59 KTLIVEAIVRETGACNVQVIGKTLV 83 KTL+ +A+ ET A ++V+G LV Sbjct: 64 KTLLAKAVATETNATFIRVVGSELV 88 >6EPC-H nrPDB Length = 433 Score = 25.0 bits (53), Expect = 7.5, Method: Composition-based stats. Identities = 12/25 (48%), Positives = 14/25 (56%) Query: 59 KTLIVEAIVRETGACNVQVIGKTLV 83 KTL A+ T AC + VIG LV Sbjct: 222 KTLCARAVANRTDACFIPVIGSELV 246 >5UJW-A nrPDB Length = 253 Score = 24.6 bits (52), Expect = 8.6, Method: Compositional matrix adjust. Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 8/54 (14%) Query: 2 NLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATED 55 NLS +Q L + +PV +G+ GV+A +EQ E H+ I+ +A D Sbjct: 151 NLSVEQ---LAKVVIAYEPVWAIGT-----GVVASLEQIQETHQFIRSLLAKVD 196 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1LN4-A nrPDB 147 7e-37 1JO0-A nrPDB 141 7e-35 1RQ8-A nrPDB 128 7e-31 Sequences not found previously or not previously below threshold: 3MN8-A nrPDB 28 1.3 2XRX-B nrPDB 27 1.8 1DEQ-B nrPDB 27 1.8 5T4Q-L nrPDB 27 2.2 5UE8-A nrPDB 27 3.0 5VFU-A nrPDB 26 3.1 1C3X-A nrPDB 26 3.1 5VHO-A nrPDB 26 3.2 6DD5-A nrPDB 26 3.3 6NYT-A nrPDB 26 3.6 3H0G-E nrPDB 26 4.3 3DRW-A nrPDB 25 5.2 3H4M-A nrPDB 25 6.2 6HE4-H nrPDB 25 7.2 6HE9-H nrPDB 25 8.5 CONVERGED! >1LN4-A nrPDB Length = 104 Score = 147 bits (372), Expect = 7e-37, Method: Composition-based stats. Identities = 95/96 (98%), Positives = 96/96 (100%) Query: 1 MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKT 60 M+LSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKT Sbjct: 1 MDLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKT 60 Query: 61 LIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLP 96 LIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLP Sbjct: 61 LIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLP 96 >1JO0-A nrPDB Length = 98 Score = 141 bits (355), Expect = 7e-35, Method: Composition-based stats. Identities = 72/95 (75%), Positives = 77/95 (81%) Query: 3 LSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKTLI 62 LSTKQKQ LKGLAH L PVV+LG NGLTEGVLAEIE AL HHELIKVK+A DRETK LI Sbjct: 3 LSTKQKQFLKGLAHHLNPVVMLGGNGLTEGVLAEIENALNHHELIKVKVAGADRETKQLI 62 Query: 63 VEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR 97 + AIVRET A VQ IG LVLYRP++E KI LPR Sbjct: 63 INAIVRETKAAQVQTIGHILVLYRPSEEAKIQLPR 97 >1RQ8-A nrPDB Length = 104 Score = 128 bits (321), Expect = 7e-31, Method: Composition-based stats. Identities = 37/95 (38%), Positives = 59/95 (62%), Gaps = 1/95 (1%) Query: 3 LSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKTLI 62 L+ KQK++L+ LAH + P+ +G G+ E ++ +I+ LE+ ELIKV + + + K + Sbjct: 2 LTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRELIKVHVLQNNFDDKKEL 61 Query: 63 VEAIVRETGACNVQVIGKTLVLYRPTKERK-ISLP 96 E + T + VQVIG +V+YR +KE K I LP Sbjct: 62 AETLSEATRSELVQVIGSMIVIYRESKENKEIELP 96 >3MN8-A nrPDB Length = 435 Score = 27.7 bits (60), Expect = 1.3, Method: Composition-based stats. Identities = 15/69 (21%), Positives = 28/69 (40%), Gaps = 16/69 (23%) Query: 31 EGVLAEIEQALEHHELIKVKIATEDRETKTLIVEAIVRETGACNV--------------Q 76 E + A +E+A + KV E + L+ I R T + N+ + Sbjct: 47 EDLFAGLEKAYPNQA--KVHFLGRSLEGRNLLALQISRNTRSRNLLTPPVKYIANMHGDE 104 Query: 77 VIGKTLVLY 85 +G+ L++Y Sbjct: 105 TVGRQLLVY 113 >2XRX-B nrPDB Length = 188 Score = 26.9 bits (58), Expect = 1.8, Method: Composition-based stats. Identities = 11/38 (28%), Positives = 20/38 (52%) Query: 58 TKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISL 95 T+ L+ IV+ET + + +LYR ER++ + Sbjct: 110 TRHLVSNVIVKETATPDTFEVNSAFILYRNRLERQVDI 147 >1DEQ-B nrPDB Length = 408 Score = 26.9 bits (58), Expect = 1.8, Method: Composition-based stats. Identities = 10/43 (23%), Positives = 19/43 (44%) Query: 30 TEGVLAEIEQALEHHELIKVKIATEDRETKTLIVEAIVRETGA 72 E V+ E LE H+L + + TK ++ +I+ + Sbjct: 82 NENVVNEYSSHLEKHQLYIDETVKNNIPTKLRVLRSILENLRS 124 >5T4Q-L nrPDB Length = 177 Score = 26.9 bits (58), Expect = 2.2, Method: Composition-based stats. Identities = 17/64 (26%), Positives = 33/64 (51%), Gaps = 2/64 (3%) Query: 6 KQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKTLIVEA 65 +Q HL+ ++ V ++ + L+E LA+I A+E KVK+ + K+++ Sbjct: 94 EQFIHLRAVSEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKL--NAKIDKSVMAGV 151 Query: 66 IVRE 69 I+R Sbjct: 152 IIRA 155 >5UE8-A nrPDB Length = 961 Score = 26.5 bits (57), Expect = 3.0, Method: Composition-based stats. Identities = 18/74 (24%), Positives = 29/74 (39%), Gaps = 10/74 (13%) Query: 5 TKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKTLIVE 64 K K+ K + L PV E E + + IKV++ ED + K+ + + Sbjct: 191 GKTKKRTKTIYGNLNPVWE-------ENFHFECHNSSDR---IKVRVWDEDDDIKSRVKQ 240 Query: 65 AIVRETGACNVQVI 78 RE+ Q I Sbjct: 241 RFKRESDDFLGQTI 254 >5VFU-A nrPDB Length = 361 Score = 26.2 bits (56), Expect = 3.1, Method: Composition-based stats. Identities = 12/25 (48%), Positives = 15/25 (60%) Query: 59 KTLIVEAIVRETGACNVQVIGKTLV 83 KTL A+ T AC ++VIG LV Sbjct: 150 KTLCARAVANRTDACFIRVIGSELV 174 >1C3X-A nrPDB Length = 266 Score = 26.2 bits (56), Expect = 3.1, Method: Composition-based stats. Identities = 23/87 (26%), Positives = 37/87 (42%), Gaps = 15/87 (17%) Query: 1 MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH----ELIKVKIATEDR 56 ++L+ L+ LAH + P L EGV A+ H+ E+ I D Sbjct: 153 VDLTDVYSPRLRELAHRVDPT-------LPEGVYAQFPGP--HYETPAEVRMAGILGADL 203 Query: 57 ETKTLIVEAIVRETGACNVQVIGKTLV 83 + +EAI C ++V+G +LV Sbjct: 204 VGMSTTLEAI--AARHCGLEVLGVSLV 228 >5VHO-A nrPDB Length = 267 Score = 26.2 bits (56), Expect = 3.2, Method: Composition-based stats. Identities = 12/25 (48%), Positives = 15/25 (60%) Query: 59 KTLIVEAIVRETGACNVQVIGKTLV 83 KTL A+ T AC ++VIG LV Sbjct: 65 KTLCARAVANRTDACFIRVIGSELV 89 >6DD5-A nrPDB Length = 678 Score = 26.2 bits (56), Expect = 3.3, Method: Composition-based stats. Identities = 10/35 (28%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Query: 33 VLAEIEQALEHHELIKVKIATEDRETKTLIVEAIV 67 VL +IE EHH++ +++ + + L++E V Sbjct: 487 VLEKIEDTFEHHQVTQIEDLSV-FDINALMLETAV 520 >6NYT-A nrPDB Length = 148 Score = 26.2 bits (56), Expect = 3.6, Method: Composition-based stats. Identities = 18/74 (24%), Positives = 29/74 (39%), Gaps = 10/74 (13%) Query: 5 TKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKTLIVE 64 K K+ K + L PV E E + + IKV++ ED + K+ + + Sbjct: 47 GKTKKRTKTIYGNLNPVWE-------ENFHFECHNSSDR---IKVRVWDEDDDIKSRVKQ 96 Query: 65 AIVRETGACNVQVI 78 RE+ Q I Sbjct: 97 RFKRESDDFLGQTI 110 >3H0G-E nrPDB Length = 210 Score = 25.8 bits (55), Expect = 4.3, Method: Composition-based stats. Identities = 12/37 (32%), Positives = 18/37 (48%) Query: 41 LEHHELIKVKIATEDRETKTLIVEAIVRETGACNVQV 77 + HHEL+ I E K L+ +RET +Q+ Sbjct: 139 ITHHELVPKHILLSPDEKKELLDRYKLRETQLPRIQL 175 >3DRW-A nrPDB Length = 474 Score = 25.4 bits (54), Expect = 5.2, Method: Composition-based stats. Identities = 20/91 (21%), Positives = 36/91 (39%), Gaps = 2/91 (2%) Query: 4 STKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHHELIKVKIATEDRETKTLIV 63 S + ++ K + + P V S G+ E +A+I L + EL E L Sbjct: 285 SVQDRKLRKKIITNILPFV--DSVGIDEAEIAQILSVLGYRELADRIFTYNRLEDSILGG 342 Query: 64 EAIVRETGACNVQVIGKTLVLYRPTKERKIS 94 I+ E +QV ++Y ++ +S Sbjct: 343 MIILDELNFEILQVHTTYYLMYITHRDNPLS 373 >3H4M-A nrPDB Length = 285 Score = 25.4 bits (54), Expect = 6.2, Method: Composition-based stats. Identities = 11/25 (44%), Positives = 17/25 (68%) Query: 59 KTLIVEAIVRETGACNVQVIGKTLV 83 KTL+ +A+ ET A ++V+G LV Sbjct: 64 KTLLAKAVATETNATFIRVVGSELV 88 >6HE4-H nrPDB Length = 267 Score = 25.0 bits (53), Expect = 7.2, Method: Composition-based stats. Identities = 9/25 (36%), Positives = 16/25 (64%) Query: 59 KTLIVEAIVRETGACNVQVIGKTLV 83 KTL+ +A+ +T A ++V+G V Sbjct: 66 KTLLAKAVANQTRATFIRVVGSEFV 90 >6HE9-H nrPDB Length = 390 Score = 25.0 bits (53), Expect = 8.5, Method: Composition-based stats. Identities = 9/25 (36%), Positives = 16/25 (64%) Query: 59 KTLIVEAIVRETGACNVQVIGKTLV 83 KTL+ +A+ +T A ++V+G V Sbjct: 180 KTLLAKAVANQTRATFIRVVGSEFV 204 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.306 0.137 0.335 Lambda K H 0.267 0.0417 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,337,133 Number of Sequences: 36641 Number of extensions: 98576 Number of successful extensions: 365 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 33 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 331 Number of HSP's gapped (non-prelim): 48 length of query: 97 length of database: 10,336,785 effective HSP length: 65 effective length of query: 32 effective length of database: 7,955,120 effective search space: 254563840 effective search space used: 254563840 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.0 bits) S2: 52 (24.6 bits)