BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P0AG99 (110 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5MG3-G nrPDB 218 6e-58 3J46-G nrPDB 124 1e-29 3DL8-E nrPDB 47 2e-06 3DIN-E nrPDB 38 0.001 3AC0-A nrPDB 30 0.17 5CH4-G nrPDB 30 0.21 3S9V-A nrPDB 27 1.7 4D02-A nrPDB 27 2.0 6MAT-A nrPDB 25 6.4 3WSY-A nrPDB 25 6.5 3DIN-A nrPDB 25 6.8 6CGB-A nrPDB 25 9.1 2A4C-A nrPDB 25 9.9 >5MG3-G nrPDB Length = 136 Score = 218 bits (554), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 110/110 (100%), Positives = 110/110 (100%) Query: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA Sbjct: 27 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 86 Query: 61 TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIPN 110 TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIPN Sbjct: 87 TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIPN 136 >3J46-G nrPDB Length = 67 Score = 124 bits (310), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 65/65 (100%), Positives = 65/65 (100%) Query: 9 FLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLATLFFIISL 68 FLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLATLFFIISL Sbjct: 2 FLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLATLFFIISL 61 Query: 69 VLGNI 73 VLGNI Sbjct: 62 VLGNI 66 >3DL8-E nrPDB Length = 107 Score = 46.6 bits (109), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 24/56 (42%), Positives = 38/56 (67%), Gaps = 1/56 (1%) Query: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMT 56 MY ALL +F+I+A+ L+ +LQ+G+G D+GA+FG G ++FG G +T+ T Sbjct: 1 MYYALLTLFVIIAVVLIISTLLQKGRG-DVGAAFGGGMGQSIFGVGGVETILTKAT 55 >3DIN-E nrPDB Length = 76 Score = 37.7 bits (86), Expect = 0.001, Method: Compositional matrix adjust. Identities = 20/69 (28%), Positives = 41/69 (59%) Query: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60 M L+V I+++ L+ ++ +Q K +++G +FG+G T+FG + ++T +L+ Sbjct: 1 MKTFFLIVHTIISVALIYMVQVQMSKFSELGGAFGSGGLHTVFGRRKGLDTGGKITLVLS 60 Query: 61 TLFFIISLV 69 LFF+ +V Sbjct: 61 VLFFVSCVV 69 >3AC0-A nrPDB Length = 845 Score = 30.4 bits (67), Expect = 0.17, Method: Composition-based stats. Identities = 26/104 (25%), Positives = 46/104 (44%), Gaps = 14/104 (13%) Query: 20 IMLQQGKGADMGASFGAGASATLFGSSGSGNFMT------------RMTALLATLFFIIS 67 + L++G+ ++ +G+G ++ L G G+G F R A LA Sbjct: 519 LTLKKGQVYNVRVEYGSGPTSGLVGEFGAGGFQAGVIKAIDDDEEIRNAAELAAKHDKAV 578 Query: 68 LVLGNINSNKTNKGSEWENLSAPAKT-EQTQPAAPAKPTSDIPN 110 L++G +N +G + EN+ P +T E + A P + I N Sbjct: 579 LIIG-LNGEWETEGYDRENMDLPKRTNELVRAVLKANPNTVIVN 621 >5CH4-G nrPDB Length = 75 Score = 30.0 bits (66), Expect = 0.21, Method: Compositional matrix adjust. Identities = 24/71 (33%), Positives = 41/71 (57%), Gaps = 3/71 (4%) Query: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60 +Y +++ +L VA LV L+++Q+ K GA G SA LF + G + R+T +L Sbjct: 4 LYTLVILFYLGVAGLLVYLVLVQEPK---QGAGDLMGGSADLFSARGVTGGLYRLTVILG 60 Query: 61 TLFFIISLVLG 71 +F ++LV+G Sbjct: 61 VVFAALALVIG 71 >3S9V-A nrPDB Length = 785 Score = 27.3 bits (59), Expect = 1.7, Method: Composition-based stats. Identities = 17/59 (28%), Positives = 25/59 (42%) Query: 44 GSSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA 102 GS G G + +LATL II+L L + KG E+ A ++ P+ Sbjct: 91 GSWGEGFYFLAYDRILATLACIITLTLWRTGETQVQKGIEFFRTQAGKMEDEADSHRPS 149 >4D02-A nrPDB Length = 479 Score = 26.9 bits (58), Expect = 2.0, Method: Composition-based stats. Identities = 10/21 (47%), Positives = 13/21 (61%) Query: 90 PAKTEQTQPAAPAKPTSDIPN 110 PAK E Q AP P S++P+ Sbjct: 437 PAKGEPMQDVAPGTPWSEVPD 457 >6MAT-A nrPDB Length = 813 Score = 25.4 bits (54), Expect = 6.4, Method: Composition-based stats. Identities = 11/26 (42%), Positives = 13/26 (50%) Query: 85 ENLSAPAKTEQTQPAAPAKPTSDIPN 110 EN++A T T P PA T PN Sbjct: 151 ENMAADTPTTSTGPVLPASSTDRQPN 176 >3WSY-A nrPDB Length = 678 Score = 25.4 bits (54), Expect = 6.5, Method: Composition-based stats. Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 6/36 (16%) Query: 64 FIISLVLGNINSNKT------NKGSEWENLSAPAKT 93 +I +L+ G++N +KG WE L APA T Sbjct: 340 YIATLINGSMNEENMRSVITFDKGGTWEFLQAPAFT 375 >3DIN-A nrPDB Length = 871 Score = 25.0 bits (53), Expect = 6.8, Method: Composition-based stats. Identities = 23/85 (27%), Positives = 39/85 (45%), Gaps = 12/85 (14%) Query: 14 IGLVGLIMLQQGKGADMGASFGAGASAT----LFGSSGSG-------NFMTRMTAL-LAT 61 + ++G I L +GK A+M G +AT L G G +++ R AL + Sbjct: 79 VQVMGGIALHEGKVAEMKTGEGKTLAATMPIYLNALIGKGVHLVTVNDYLARRDALWMGP 138 Query: 62 LFFIISLVLGNINSNKTNKGSEWEN 86 ++ + L +G INS + W+N Sbjct: 139 VYLFLGLRVGVINSLGKSYEVVWKN 163 >6CGB-A nrPDB Length = 207 Score = 24.6 bits (52), Expect = 9.1, Method: Compositional matrix adjust. Identities = 17/63 (26%), Positives = 26/63 (41%) Query: 45 SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKP 104 SG GN ++ A F+I GNI++ KT E + A+ P +P Sbjct: 29 DSGDGNIKYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQYTLMAQAVDRDTNRPLEP 88 Query: 105 TSD 107 S+ Sbjct: 89 PSE 91 >2A4C-A nrPDB Length = 99 Score = 24.6 bits (52), Expect = 9.9, Method: Compositional matrix adjust. Identities = 17/63 (26%), Positives = 26/63 (41%) Query: 45 SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKP 104 SG GN ++ A F+I GNI++ KT E + A+ P +P Sbjct: 30 DSGDGNIKYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQYTLMAQAVDRDTNRPLEP 89 Query: 105 TSD 107 S+ Sbjct: 90 PSE 92 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5MG3-G nrPDB 170 1e-43 3DIN-E nrPDB 94 2e-20 3J46-G nrPDB 79 6e-16 3DL8-E nrPDB 67 2e-12 Sequences not found previously or not previously below threshold: 5CH4-G nrPDB 36 0.004 4D02-A nrPDB 27 2.3 1SNN-A nrPDB 27 2.9 1H1I-A nrPDB 26 3.2 5HAF-A nrPDB 26 4.2 CONVERGED! >5MG3-G nrPDB Length = 136 Score = 170 bits (430), Expect = 1e-43, Method: Composition-based stats. Identities = 110/110 (100%), Positives = 110/110 (100%) Query: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA Sbjct: 27 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 86 Query: 61 TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIPN 110 TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIPN Sbjct: 87 TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIPN 136 >3DIN-E nrPDB Length = 76 Score = 93.6 bits (231), Expect = 2e-20, Method: Composition-based stats. Identities = 20/69 (28%), Positives = 41/69 (59%) Query: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60 M L+V I+++ L+ ++ +Q K +++G +FG+G T+FG + ++T +L+ Sbjct: 1 MKTFFLIVHTIISVALIYMVQVQMSKFSELGGAFGSGGLHTVFGRRKGLDTGGKITLVLS 60 Query: 61 TLFFIISLV 69 LFF+ +V Sbjct: 61 VLFFVSCVV 69 >3J46-G nrPDB Length = 67 Score = 78.6 bits (192), Expect = 6e-16, Method: Composition-based stats. Identities = 65/65 (100%), Positives = 65/65 (100%) Query: 9 FLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLATLFFIISL 68 FLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLATLFFIISL Sbjct: 2 FLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLATLFFIISL 61 Query: 69 VLGNI 73 VLGNI Sbjct: 62 VLGNI 66 >3DL8-E nrPDB Length = 107 Score = 67.0 bits (162), Expect = 2e-12, Method: Composition-based stats. Identities = 24/56 (42%), Positives = 38/56 (67%), Gaps = 1/56 (1%) Query: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMT 56 MY ALL +F+I+A+ L+ +LQ+G+G D+GA+FG G ++FG G +T+ T Sbjct: 1 MYYALLTLFVIIAVVLIISTLLQKGRG-DVGAAFGGGMGQSIFGVGGVETILTKAT 55 >5CH4-G nrPDB Length = 75 Score = 36.2 bits (82), Expect = 0.004, Method: Composition-based stats. Identities = 24/71 (33%), Positives = 41/71 (57%), Gaps = 3/71 (4%) Query: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60 +Y +++ +L VA LV L+++Q+ K GA G SA LF + G + R+T +L Sbjct: 4 LYTLVILFYLGVAGLLVYLVLVQEPK---QGAGDLMGGSADLFSARGVTGGLYRLTVILG 60 Query: 61 TLFFIISLVLG 71 +F ++LV+G Sbjct: 61 VVFAALALVIG 71 >4D02-A nrPDB Length = 479 Score = 26.6 bits (57), Expect = 2.3, Method: Composition-based stats. Identities = 10/21 (47%), Positives = 13/21 (61%) Query: 90 PAKTEQTQPAAPAKPTSDIPN 110 PAK E Q AP P S++P+ Sbjct: 437 PAKGEPMQDVAPGTPWSEVPD 457 >1SNN-A nrPDB Length = 227 Score = 26.6 bits (57), Expect = 2.9, Method: Composition-based stats. Identities = 21/80 (26%), Positives = 36/80 (45%), Gaps = 13/80 (16%) Query: 21 MLQQGKGADMGASFGAGASATLF----GSSGSGNFMTRMTALLATL-----FFIISLVLG 71 ++++G+ D G F + S TL G + T MT LA L I ++G Sbjct: 129 LVKEGRFNDFGKEFRSPGSVTLLRAAEGLVKNRQGHTEMTVALAELANLVPITTICEMMG 188 Query: 72 N----INSNKTNKGSEWENL 87 + ++ N+T + +E NL Sbjct: 189 DDGNAMSKNETKRYAEKHNL 208 >1H1I-A nrPDB Length = 350 Score = 26.2 bits (56), Expect = 3.2, Method: Composition-based stats. Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 6/74 (8%) Query: 18 GLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLATLFFIISLVLGNINSNK 77 G +++Q G A G+G A + G F A + + F+ S G ++ N Sbjct: 281 GRVVVQIGDYA--ATELGSGDVAFI---PGGVEFKYYSEAYFSKVLFVSSGSDG-LDQNL 334 Query: 78 TNKGSEWENLSAPA 91 N G EW ++S PA Sbjct: 335 VNGGEEWSSVSFPA 348 >5HAF-A nrPDB Length = 338 Score = 25.8 bits (55), Expect = 4.2, Method: Composition-based stats. Identities = 11/28 (39%), Positives = 17/28 (60%), Gaps = 1/28 (3%) Query: 66 ISLVLGNI-NSNKTNKGSEWENLSAPAK 92 LVL I N++ T +EW+ L AP++ Sbjct: 84 ACLVLHQIANNDITKDNTEWKKLHAPSR 111 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.134 0.364 Lambda K H 0.267 0.0417 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,874,676 Number of Sequences: 36641 Number of extensions: 124704 Number of successful extensions: 358 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 24 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 329 Number of HSP's gapped (non-prelim): 35 length of query: 110 length of database: 10,336,785 effective HSP length: 76 effective length of query: 34 effective length of database: 7,552,069 effective search space: 256770346 effective search space used: 256770346 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 52 (24.6 bits)