BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P0AFU8 (213 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1I8D-A nrPDB 440 e-124 1KZL-A nrPDB 131 2e-31 3DDY-A nrPDB 80 5e-16 2JER-A nrPDB 30 0.93 5UYY-A nrPDB 28 4.3 1C8N-A nrPDB 27 4.9 6FOC-A nrPDB 27 7.0 4IP5-A nrPDB 27 7.3 3IJ3-A nrPDB 27 7.7 3V10-A nrPDB 27 8.1 4RSI-B nrPDB 27 8.2 >1I8D-A nrPDB Length = 213 Score = 440 bits (1132), Expect = e-124, Method: Compositional matrix adjust. Identities = 213/213 (100%), Positives = 213/213 (100%) Query: 1 MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAHNGCCLTVTEINGNHVS 60 MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAHNGCCLTVTEINGNHVS Sbjct: 1 MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAHNGCCLTVTEINGNHVS 60 Query: 61 FDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQ 120 FDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQ Sbjct: 61 FDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQ 120 Query: 121 IWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARV 180 IWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARV Sbjct: 121 IWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARV 180 Query: 181 NIEIDPQTQAVVDTVERVLAARENAMNQPGTEA 213 NIEIDPQTQAVVDTVERVLAARENAMNQPGTEA Sbjct: 181 NIEIDPQTQAVVDTVERVLAARENAMNQPGTEA 213 >1KZL-A nrPDB Length = 208 Score = 131 bits (329), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 75/189 (39%), Positives = 110/189 (58%), Gaps = 8/189 (4%) Query: 1 MFTGIVQ--GTAKLV--SIDEKPNFRTHVVELPDHMLDGLETGASVAHNGCCLTVTEING 56 MFTG+V+ G K V +ID + +E P +LD TG S+A NG CLTVT+ + Sbjct: 1 MFTGLVEAIGVVKDVQGTIDNGFAMK---IEAP-QILDDCHTGDSIAVNGTCLTVTDFDR 56 Query: 57 NHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSE 116 H + + E+LR+TNLG K GD VN+ERA S +GGH + GH+ T AE+ + Sbjct: 57 YHFTVGIAPESLRLTNLGQCKAGDPVNLERAVLSSTRMGGHFVQGHVDTVAEIVEKKQDG 116 Query: 117 NNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKL 176 F+ +D ++KYI+YKG+I +DG SLT+ V + F + +I T + + KK + Sbjct: 117 EAIDFTFRPRDPFVLKYIVYKGYIALDGTSLTITHVDDSTFSIMMISYTQSKVIMAKKNV 176 Query: 177 GARVNIEID 185 G VN+E+D Sbjct: 177 GDLVNVEVD 185 >3DDY-A nrPDB Length = 186 Score = 80.5 bits (197), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 57/184 (30%), Positives = 92/184 (50%), Gaps = 2/184 (1%) Query: 1 MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAHNGCCLTVTEINGNHVS 60 MF GIVQG + SI + + + H + P+ M ++ + NGC TV I G+ V Sbjct: 1 MFRGIVQGRGVIRSISKSEDSQRHGIAFPEGMFQLVDVDTVMLVNGCSNTVVRILGDMVY 60 Query: 61 FDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQ 120 FD+ + L T LK GD VN+E KF + +G ++G+I TA VA I ++ Sbjct: 61 FDI-DQALGTTTFDGLKEGDQVNLEIHPKFGEVVGRGGLTGNIKGTALVAAIEENDAGFS 119 Query: 121 IWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARV 180 + + L + + K IGIDGISL + +++ + ++ + L T + +V Sbjct: 120 VLIDIPKG-LAENLTVKDDIGIDGISLPITDMSDSIITLNYSRDLLASTNIASLAKDVKV 178 Query: 181 NIEI 184 N+EI Sbjct: 179 NVEI 182 >2JER-A nrPDB Length = 389 Score = 29.6 bits (65), Expect = 0.93, Method: Compositional matrix adjust. Identities = 21/70 (30%), Positives = 33/70 (47%), Gaps = 8/70 (11%) Query: 77 KVGDWVNVERAAKFSDEIGGHLMSGHI------MTTAEVAKILTSENNRQIWFKVQDS-- 128 K+ D++NVE+ D I +GH+ + EVA I T + N + QD+ Sbjct: 193 KLCDYLNVEKVLWLGDGIDPEETNGHVDDVACFIAPGEVACIYTEDQNSPFYEAAQDAYQ 252 Query: 129 QLMKYILYKG 138 +L+K KG Sbjct: 253 RLLKMTDAKG 262 >5UYY-A nrPDB Length = 381 Score = 27.7 bits (60), Expect = 4.3, Method: Compositional matrix adjust. Identities = 26/61 (42%), Positives = 33/61 (54%), Gaps = 6/61 (9%) Query: 60 SFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEI---GGHLMSGHIMTTAEVAKILTSE 116 SF L +E + +T++G K G +N E A FS EI GGH M+G T E AK E Sbjct: 98 SFHL-REDVIVTDVGSTK-GSIMN-EAEALFSKEISFIGGHPMAGSHKTGVESAKAHLFE 154 Query: 117 N 117 N Sbjct: 155 N 155 >1C8N-A nrPDB Length = 276 Score = 27.3 bits (59), Expect = 4.9, Method: Compositional matrix adjust. Identities = 13/32 (40%), Positives = 19/32 (59%) Query: 124 KVQDSQLMKYILYKGFIGIDGISLTVGEVTPT 155 +VQ SQ K I + + G DG ++ +VTPT Sbjct: 169 RVQLSQTYKAINFPPYAGYDGAAILNTDVTPT 200 >6FOC-A nrPDB Length = 548 Score = 26.9 bits (58), Expect = 7.0, Method: Compositional matrix adjust. Identities = 11/18 (61%), Positives = 14/18 (77%) Query: 84 VERAAKFSDEIGGHLMSG 101 +ER AK SDE+GG M+G Sbjct: 309 LERCAKLSDELGGGSMTG 326 >4IP5-A nrPDB Length = 425 Score = 26.9 bits (58), Expect = 7.3, Method: Compositional matrix adjust. Identities = 18/62 (29%), Positives = 32/62 (51%), Gaps = 5/62 (8%) Query: 153 TPTRFCV-HLIPETLERTTLGKKKLGARVNIEIDP----QTQAVVDTVERVLAARENAMN 207 T T+F V + + + R T+ +++LG +NI ID + +D V R+ AR + Sbjct: 213 THTKFKVGRDLSDDIRRLTIAREELGEDMNIMIDANQVWEVDEAIDWVNRLAFARPLFIE 272 Query: 208 QP 209 +P Sbjct: 273 EP 274 >3IJ3-A nrPDB Length = 482 Score = 26.6 bits (57), Expect = 7.7, Method: Compositional matrix adjust. Identities = 9/32 (28%), Positives = 18/32 (56%) Query: 122 WFKVQDSQLMKYILYKGFIGIDGISLTVGEVT 153 W QD++ +++ GF+G+ G ++ E T Sbjct: 51 WLDTQDARTQQWVKASGFVGLAGTICSIPEST 82 >3V10-A nrPDB Length = 321 Score = 26.6 bits (57), Expect = 8.1, Method: Compositional matrix adjust. Identities = 11/31 (35%), Positives = 22/31 (70%), Gaps = 2/31 (6%) Query: 45 NGCCLTVTEI--NGNHVSFDLMKETLRITNL 73 NG +T+ E+ NG+H+ F+ M++ L++ N+ Sbjct: 81 NGKEITLGELTFNGSHIHFEFMEDVLQLENV 111 >4RSI-B nrPDB Length = 397 Score = 26.6 bits (57), Expect = 8.2, Method: Compositional matrix adjust. Identities = 13/30 (43%), Positives = 18/30 (60%) Query: 175 KLGARVNIEIDPQTQAVVDTVERVLAAREN 204 KLG + ++D T VD +ER L+ REN Sbjct: 280 KLGTNQSDKVDDYTPEEVDKIERELSEREN 309 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1I8D-A nrPDB 330 2e-91 1KZL-A nrPDB 239 6e-64 3DDY-A nrPDB 229 5e-61 Sequences not found previously or not previously below threshold: 4BLN-A nrPDB 31 0.44 2D0V-A nrPDB 29 1.9 4HO3-A nrPDB 29 2.3 1LRW-A nrPDB 28 2.5 2F8L-A nrPDB 27 4.5 5WLC-LH nrPDB 27 4.7 1H4I-A nrPDB 27 5.1 4RM4-A nrPDB 27 8.8 CONVERGED! >1I8D-A nrPDB Length = 213 Score = 330 bits (846), Expect = 2e-91, Method: Composition-based stats. Identities = 213/213 (100%), Positives = 213/213 (100%) Query: 1 MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAHNGCCLTVTEINGNHVS 60 MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAHNGCCLTVTEINGNHVS Sbjct: 1 MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAHNGCCLTVTEINGNHVS 60 Query: 61 FDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQ 120 FDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQ Sbjct: 61 FDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQ 120 Query: 121 IWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARV 180 IWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARV Sbjct: 121 IWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARV 180 Query: 181 NIEIDPQTQAVVDTVERVLAARENAMNQPGTEA 213 NIEIDPQTQAVVDTVERVLAARENAMNQPGTEA Sbjct: 181 NIEIDPQTQAVVDTVERVLAARENAMNQPGTEA 213 >1KZL-A nrPDB Length = 208 Score = 239 bits (610), Expect = 6e-64, Method: Composition-based stats. Identities = 75/189 (39%), Positives = 110/189 (58%), Gaps = 8/189 (4%) Query: 1 MFTGIVQ--GTAKLV--SIDEKPNFRTHVVELPDHMLDGLETGASVAHNGCCLTVTEING 56 MFTG+V+ G K V +ID + +E P +LD TG S+A NG CLTVT+ + Sbjct: 1 MFTGLVEAIGVVKDVQGTIDNGFAMK---IEAP-QILDDCHTGDSIAVNGTCLTVTDFDR 56 Query: 57 NHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSE 116 H + + E+LR+TNLG K GD VN+ERA S +GGH + GH+ T AE+ + Sbjct: 57 YHFTVGIAPESLRLTNLGQCKAGDPVNLERAVLSSTRMGGHFVQGHVDTVAEIVEKKQDG 116 Query: 117 NNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKL 176 F+ +D ++KYI+YKG+I +DG SLT+ V + F + +I T + + KK + Sbjct: 117 EAIDFTFRPRDPFVLKYIVYKGYIALDGTSLTITHVDDSTFSIMMISYTQSKVIMAKKNV 176 Query: 177 GARVNIEID 185 G VN+E+D Sbjct: 177 GDLVNVEVD 185 >3DDY-A nrPDB Length = 186 Score = 229 bits (585), Expect = 5e-61, Method: Composition-based stats. Identities = 57/184 (30%), Positives = 92/184 (50%), Gaps = 2/184 (1%) Query: 1 MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAHNGCCLTVTEINGNHVS 60 MF GIVQG + SI + + + H + P+ M ++ + NGC TV I G+ V Sbjct: 1 MFRGIVQGRGVIRSISKSEDSQRHGIAFPEGMFQLVDVDTVMLVNGCSNTVVRILGDMVY 60 Query: 61 FDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQ 120 FD+ + L T LK GD VN+E KF + +G ++G+I TA VA I ++ Sbjct: 61 FDI-DQALGTTTFDGLKEGDQVNLEIHPKFGEVVGRGGLTGNIKGTALVAAIEENDAGFS 119 Query: 121 IWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARV 180 + + L + + K IGIDGISL + +++ + ++ + L T + +V Sbjct: 120 VLIDIPKG-LAENLTVKDDIGIDGISLPITDMSDSIITLNYSRDLLASTNIASLAKDVKV 178 Query: 181 NIEI 184 N+EI Sbjct: 179 NVEI 182 >4BLN-A nrPDB Length = 332 Score = 30.8 bits (68), Expect = 0.44, Method: Composition-based stats. Identities = 22/116 (18%), Positives = 37/116 (31%), Gaps = 18/116 (15%) Query: 36 LETGASVAHNGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVN------VERAAK 89 G + A+ CC V F ++ + ++ + G+ V+ A Sbjct: 4 CADGRTTANAACC----------VLFPILDD-IQENLFDGAQCGEEVHESLRLTFHDAIG 52 Query: 90 FSDEIGGHLMSGHIMTTAEVAKILTSENNRQIWFKVQDSQLMK-YILYKGFIGIDG 144 FS +GG G I+T + + Q + K I FI G Sbjct: 53 FSPTLGGGGADGSIITFDTIETNFPANAGIDEIVSAQKPFVAKHNISAGDFIQFAG 108 >2D0V-A nrPDB Length = 597 Score = 28.5 bits (62), Expect = 1.9, Method: Composition-based stats. Identities = 14/50 (28%), Positives = 24/50 (48%), Gaps = 3/50 (6%) Query: 45 NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEI 94 NG T+ NG+ +S D + +T+ + DLK G V R ++ + Sbjct: 332 NGIVYTLDRENGDLISADKLDDTVNVFKTVDLKTGLPV---RDPEYGTRM 378 >4HO3-A nrPDB Length = 297 Score = 28.5 bits (62), Expect = 2.3, Method: Composition-based stats. Identities = 29/116 (25%), Positives = 48/116 (41%), Gaps = 23/116 (19%) Query: 85 ERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDG 144 E +F +GG G + + + +S D +I+ + FIG D Sbjct: 56 EDTPRFEQLLGG----GSELGISLSYAVQSS----------PDGLAQAFIIGEEFIGDDN 101 Query: 145 ISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARV---NIEIDPQTQAVVDTVER 197 ++L +G F H E L+R +K GA + N++ DPQ VV+ E+ Sbjct: 102 VALVLG---DNIFYGHGFTELLQRA--ANRKSGATIFGYNVK-DPQRFGVVEFDEK 151 >1LRW-A nrPDB Length = 600 Score = 28.1 bits (61), Expect = 2.5, Method: Composition-based stats. Identities = 14/50 (28%), Positives = 21/50 (42%), Gaps = 3/50 (6%) Query: 45 NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEI 94 NG T+ NG+ +S D M +T+ L G V R +F + Sbjct: 332 NGIVYTLDRTNGDLISADKMDDTVNWVKEVQLDTGLPV---RDPEFGTRM 378 >2F8L-A nrPDB Length = 344 Score = 27.4 bits (59), Expect = 4.5, Method: Composition-based stats. Identities = 20/128 (15%), Positives = 46/128 (35%), Gaps = 8/128 (6%) Query: 66 ETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQIWFKV 125 + L++ L +K G VN + I +L+ I V+ + + + Sbjct: 89 KGLQLALLKGMKHGIQVNHQMTPDSIGFIVAYLLEKVIQKKKNVSILDPACGTANLL--- 145 Query: 126 QDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARVNIEID 185 + ++ + KG + + + +V + L+ L+R + N+ +D Sbjct: 146 --TTVINQLELKGDVDVHASGV---DVDDLLISLALVGADLQRQKMTLLHQDGLANLLVD 200 Query: 186 PQTQAVVD 193 P + D Sbjct: 201 PVDVVISD 208 >5WLC-LH nrPDB Length = 896 Score = 27.4 bits (59), Expect = 4.7, Method: Composition-based stats. Identities = 7/42 (16%), Positives = 18/42 (42%) Query: 145 ISLTVGEVTPTRFCVHLIPETLERTTLGKKKLGARVNIEIDP 186 IS + + + ++ + R + ++ N+EI+P Sbjct: 403 ISAMNTKNSNSITSLNHSKKKQSRKLIKSRRQDFTTNVEINP 444 >1H4I-A nrPDB Length = 599 Score = 27.4 bits (59), Expect = 5.1, Method: Composition-based stats. Identities = 13/50 (26%), Positives = 23/50 (46%), Gaps = 3/50 (6%) Query: 45 NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEI 94 NG T+ +G VS + + +T+ + DLK G V R ++ + Sbjct: 332 NGIVYTLDRTDGALVSANKLDDTVNVFKSVDLKTGQPV---RDPEYGTRM 378 >4RM4-A nrPDB Length = 396 Score = 26.6 bits (57), Expect = 8.8, Method: Composition-based stats. Identities = 15/46 (32%), Positives = 19/46 (41%), Gaps = 2/46 (4%) Query: 84 VERAAKFSDEIGGHLMSGHIMTTAEVAKILTSENNRQ--IWFKVQD 127 + R AK EIGGHL+ M A VA E F ++ Sbjct: 289 LRRIAKRDTEIGGHLIKEGDMVLAFVASANRDEAKFDRPHMFDIRR 334 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.132 0.323 Lambda K H 0.267 0.0404 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,910,751 Number of Sequences: 36641 Number of extensions: 233401 Number of successful extensions: 771 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 740 Number of HSP's gapped (non-prelim): 29 length of query: 213 length of database: 10,336,785 effective HSP length: 93 effective length of query: 120 effective length of database: 6,929,172 effective search space: 831500640 effective search space used: 831500640 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.6 bits)