BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P0AD59 (157 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1GPQ-A nrPDB 272 4e-74 4PS6-A nrPDB 35 0.017 5OQL-L nrPDB 29 0.83 4V61-BI nrPDB 27 4.6 >1GPQ-A nrPDB Length = 135 Score = 272 bits (696), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 128/129 (99%), Positives = 129/129 (100%) Query: 29 QDDLTISSLAKGETTKAAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPH 88 QDDLTISSLAKGETTKAAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPH Sbjct: 1 QDDLTISSLAKGETTKAAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPH 60 Query: 89 DCGSQRIAVMWSEKSNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLE 148 DCGSQRIAVMWSEKSNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLE Sbjct: 61 DCGSQRIAVMWSEKSNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLE 120 Query: 149 NHPDGFNFK 157 NHPDGFNF+ Sbjct: 121 NHPDGFNFR 129 >4PS6-A nrPDB Length = 130 Score = 34.7 bits (78), Expect = 0.017, Method: Compositional matrix adjust. Identities = 20/78 (25%), Positives = 40/78 (51%), Gaps = 2/78 (2%) Query: 44 KAAFNQMVQGHK-LPAWVMKGGTYTPAQTVTLGD-ETYQVMSACKPHDCGSQRIAVMWSE 101 KA ++ M +G +P WV + T + + Y + ++CKPHDCG+ R+ V + Sbjct: 17 KATWHAMFKGESDVPKWVSDASGPSSPSTSLSLEGQPYVLANSCKPHDCGNNRLLVAFRG 76 Query: 102 KSNQMTGLFSTIDEKTSQ 119 + GL ++ ++ ++ Sbjct: 77 DKSAAYGLQVSLPDEPAE 94 >5OQL-L nrPDB Length = 557 Score = 29.3 bits (64), Expect = 0.83, Method: Compositional matrix adjust. Identities = 21/72 (29%), Positives = 30/72 (41%), Gaps = 16/72 (22%) Query: 40 GETTKAAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMW 99 G+ T+A + HK P WV K ++P + TL MS C + +W Sbjct: 129 GQGTRAVILKTWHIHKQPVWVTK---WSPTELTTL-------MS------CSDDKTVRLW 172 Query: 100 SEKSNQMTGLFS 111 SN T LF+ Sbjct: 173 DLPSNDPTRLFT 184 >4V61-BI nrPDB Length = 223 Score = 26.6 bits (57), Expect = 4.6, Method: Compositional matrix adjust. Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 7/48 (14%) Query: 100 SEKSNQMTGLFSTIDE-------KTSQEKLTWLNVNDALSIDGKTVLF 140 + ++NQM GLF T+ + K ++KL + V ++DGK ++ Sbjct: 101 TRRANQMHGLFRTLTDNMVVGVSKGFEKKLILVGVGYRATVDGKELVL 148 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1GPQ-A nrPDB 268 1e-72 Sequences not found previously or not previously below threshold: 4PS6-A nrPDB 37 0.003 5OQL-L nrPDB 30 0.31 3CHX-A nrPDB 27 2.9 2MP4-A nrPDB 27 3.7 4V61-BI nrPDB 27 3.9 5XFM-A nrPDB 26 8.3 2C3Q-A nrPDB 26 8.3 CONVERGED! >1GPQ-A nrPDB Length = 135 Score = 268 bits (684), Expect = 1e-72, Method: Composition-based stats. Identities = 128/129 (99%), Positives = 129/129 (100%) Query: 29 QDDLTISSLAKGETTKAAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPH 88 QDDLTISSLAKGETTKAAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPH Sbjct: 1 QDDLTISSLAKGETTKAAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPH 60 Query: 89 DCGSQRIAVMWSEKSNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLE 148 DCGSQRIAVMWSEKSNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLE Sbjct: 61 DCGSQRIAVMWSEKSNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLE 120 Query: 149 NHPDGFNFK 157 NHPDGFNF+ Sbjct: 121 NHPDGFNFR 129 >4PS6-A nrPDB Length = 130 Score = 37.4 bits (85), Expect = 0.003, Method: Composition-based stats. Identities = 20/78 (25%), Positives = 40/78 (51%), Gaps = 2/78 (2%) Query: 44 KAAFNQMVQGHK-LPAWVMKGGTYTPAQTVTLGD-ETYQVMSACKPHDCGSQRIAVMWSE 101 KA ++ M +G +P WV + T + + Y + ++CKPHDCG+ R+ V + Sbjct: 17 KATWHAMFKGESDVPKWVSDASGPSSPSTSLSLEGQPYVLANSCKPHDCGNNRLLVAFRG 76 Query: 102 KSNQMTGLFSTIDEKTSQ 119 + GL ++ ++ ++ Sbjct: 77 DKSAAYGLQVSLPDEPAE 94 >5OQL-L nrPDB Length = 557 Score = 30.4 bits (67), Expect = 0.31, Method: Composition-based stats. Identities = 21/72 (29%), Positives = 30/72 (41%), Gaps = 16/72 (22%) Query: 40 GETTKAAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMW 99 G+ T+A + HK P WV K ++P + TL MS C + +W Sbjct: 129 GQGTRAVILKTWHIHKQPVWVTK---WSPTELTTL-------MS------CSDDKTVRLW 172 Query: 100 SEKSNQMTGLFS 111 SN T LF+ Sbjct: 173 DLPSNDPTRLFT 184 >3CHX-A nrPDB Length = 392 Score = 27.3 bits (59), Expect = 2.9, Method: Composition-based stats. Identities = 16/54 (29%), Positives = 27/54 (50%) Query: 101 EKSNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGF 154 EKS Q T++ + T LNVN+++ + GK +F+A ++ N F Sbjct: 3 EKSQQAFLRMRTLNWYDVKWSKTSLNVNESMVLSGKVHVFSAWPQAVANPKSSF 56 >2MP4-A nrPDB Length = 165 Score = 27.0 bits (58), Expect = 3.7, Method: Composition-based stats. Identities = 13/35 (37%), Positives = 22/35 (62%) Query: 21 VIATSAMAQDDLTISSLAKGETTKAAFNQMVQGHK 55 VI +A+ QD L I+ +++KAAF++ V+ K Sbjct: 37 VIVEAAVTQDQLGITGDDYDDSSKAAFDKFVEDVK 71 >4V61-BI nrPDB Length = 223 Score = 27.0 bits (58), Expect = 3.9, Method: Composition-based stats. Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 7/48 (14%) Query: 100 SEKSNQMTGLFSTIDE-------KTSQEKLTWLNVNDALSIDGKTVLF 140 + ++NQM GLF T+ + K ++KL + V ++DGK ++ Sbjct: 101 TRRANQMHGLFRTLTDNMVVGVSKGFEKKLILVGVGYRATVDGKELVL 148 >5XFM-A nrPDB Length = 642 Score = 25.8 bits (55), Expect = 8.3, Method: Composition-based stats. Identities = 16/60 (26%), Positives = 28/60 (46%), Gaps = 6/60 (10%) Query: 34 ISSLAKGETTKAAFNQMVQ----GHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHD 89 +S +++G AF + G PA+ + G T P +T+T+GD ++ P D Sbjct: 223 LSDVSEGNLYTVAFPMAEENNGNGTVAPAFALPGAT--PWRTITVGDHLKPIVETTVPWD 280 >2C3Q-A nrPDB Length = 247 Score = 25.8 bits (55), Expect = 8.3, Method: Composition-based stats. Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 2/44 (4%) Query: 31 DLTISSLAKGETTKAAFNQMVQGHKLPAWVMKGGTYTPAQTVTL 74 +L I L KG+ AF Q+ K+PA +K G +T ++V + Sbjct: 36 ELRIVDLIKGQHLSDAFAQVNPLKKVPA--LKDGDFTLTESVAI 77 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.133 0.398 Lambda K H 0.267 0.0407 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,386,411 Number of Sequences: 36641 Number of extensions: 238019 Number of successful extensions: 563 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 556 Number of HSP's gapped (non-prelim): 17 length of query: 157 length of database: 10,336,785 effective HSP length: 89 effective length of query: 68 effective length of database: 7,075,736 effective search space: 481150048 effective search space used: 481150048 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 55 (25.8 bits)