BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P0ABU7 (244 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5SV0-B nrPDB 493 e-140 5OCM-A nrPDB 32 0.32 5ZOV-A nrPDB 29 2.2 4DBL-A nrPDB 28 2.6 4FG0-A nrPDB 28 3.2 1ZVD-A nrPDB 27 7.9 3OP1-A nrPDB 27 8.7 >5SV0-B nrPDB Length = 244 Score = 493 bits (1268), Expect = e-140, Method: Compositional matrix adjust. Identities = 244/244 (100%), Positives = 244/244 (100%) Query: 1 MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSKSVEFFNQKRRLKREQQ 60 MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSKSVEFFNQKRRLKREQQ Sbjct: 1 MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSKSVEFFNQKRRLKREQQ 60 Query: 61 LLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSDDNEGIKERTSFRLERRVA 120 LLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSDDNEGIKERTSFRLERRVA Sbjct: 61 LLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSDDNEGIKERTSFRLERRVA 120 Query: 121 AVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIAQTQTTNLAVVAPGIAEALLA 180 AVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIAQTQTTNLAVVAPGIAEALLA Sbjct: 121 AVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIAQTQTTNLAVVAPGIAEALLA 180 Query: 181 TAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLLLQSRDLDLEASAAAHPVRVAQK 240 TAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLLLQSRDLDLEASAAAHPVRVAQK Sbjct: 181 TAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLLLQSRDLDLEASAAAHPVRVAQK 240 Query: 241 LRAG 244 LRAG Sbjct: 241 LRAG 244 >5OCM-A nrPDB Length = 291 Score = 31.6 bits (70), Expect = 0.32, Method: Compositional matrix adjust. Identities = 19/56 (33%), Positives = 26/56 (46%), Gaps = 8/56 (14%) Query: 124 RQMGRGNGYLATIGAISPF--VGLFGTVWGIMNSF------IGIAQTQTTNLAVVA 171 R +G G YL +S V L G +WG +NSF +G A+ + T A A Sbjct: 150 RSLGAGTTYLGADHGLSSLYDVALLGIMWGTLNSFLHGAALLGTAKVEATTFAPFA 205 >5ZOV-A nrPDB Length = 465 Score = 28.9 bits (63), Expect = 2.2, Method: Compositional matrix adjust. Identities = 30/115 (26%), Positives = 50/115 (43%), Gaps = 26/115 (22%) Query: 97 LSEGSDDNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSF 156 ++E S+ +GI ER + V A+ A I A SP +FG +WG + F Sbjct: 291 VAELSEAFKGISERV---IPNSVLAID---------CAAIYAFSPNAMVFGFMWGAIGQF 338 Query: 157 IGIAQTQ-TTNLAVVAPGIAEALLATA-IGLVAAIPAVVIYNVFARQIGGFKAML 209 + + ++ ++ PG + A IG VFA Q GG+K+++ Sbjct: 339 VAVGLLLGFSSPILIIPGFIPMFFSNATIG------------VFANQFGGWKSVM 381 >4DBL-A nrPDB Length = 349 Score = 28.5 bits (62), Expect = 2.6, Method: Compositional matrix adjust. Identities = 11/28 (39%), Positives = 22/28 (78%) Query: 21 IVVKCVMIGLILASVVTWAIFFSKSVEF 48 +++ V +G+I ++++TWAI+FS SV+ Sbjct: 168 LLLAGVALGIISSALMTWAIYFSTSVDL 195 >4FG0-A nrPDB Length = 407 Score = 28.1 bits (61), Expect = 3.2, Method: Compositional matrix adjust. Identities = 21/67 (31%), Positives = 26/67 (38%), Gaps = 2/67 (2%) Query: 141 PFVGLFGTVWGIMNSFIGIAQTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFAR 200 P+ T W + + + T VVA G E L TA L AIPA V + Sbjct: 224 PWTSPATTDWRNRETLVEFEEPHATKQTVVALGSQEGALHTA--LAGAIPATVSSSTLTL 281 Query: 201 QIGGFKA 207 Q G K Sbjct: 282 QSGHLKC 288 >1ZVD-A nrPDB Length = 380 Score = 26.9 bits (58), Expect = 7.9, Method: Compositional matrix adjust. Identities = 17/64 (26%), Positives = 33/64 (51%), Gaps = 4/64 (6%) Query: 33 ASVVTWAIFFSKSVEFFNQKRRLKREQQLLAEAR-SLNQANDIAADFGSKSLSLHLLNEA 91 +++V W F K+VEFF+++RR + Q + +R L + G + ++H ++ Sbjct: 282 SNIVKW---FWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDAC 338 Query: 92 QNEL 95 N L Sbjct: 339 TNNL 342 >3OP1-A nrPDB Length = 308 Score = 26.9 bits (58), Expect = 8.7, Method: Compositional matrix adjust. Identities = 18/63 (28%), Positives = 29/63 (46%), Gaps = 5/63 (7%) Query: 47 EFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSDDNEG 106 E F + R+ L + N++ IA + L LH+LN A+ E +L EG Sbjct: 38 ELFRVANKAARKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKR-----EG 92 Query: 107 IKE 109 ++E Sbjct: 93 VEE 95 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5SV0-B nrPDB 479 e-136 Sequences not found previously or not previously below threshold: 5OCM-A nrPDB 33 0.13 5ZOV-A nrPDB 32 0.37 4DBL-A nrPDB 30 1.1 1ZVD-A nrPDB 29 1.5 6FX4-A nrPDB 29 2.1 4FG0-A nrPDB 28 3.4 4U14-A nrPDB 27 6.6 3OP1-A nrPDB 27 9.4 4GAX-A nrPDB 27 9.6 2ONJ-A nrPDB 27 9.8 CONVERGED! >5SV0-B nrPDB Length = 244 Score = 479 bits (1233), Expect = e-136, Method: Composition-based stats. Identities = 244/244 (100%), Positives = 244/244 (100%) Query: 1 MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSKSVEFFNQKRRLKREQQ 60 MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSKSVEFFNQKRRLKREQQ Sbjct: 1 MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSKSVEFFNQKRRLKREQQ 60 Query: 61 LLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSDDNEGIKERTSFRLERRVA 120 LLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSDDNEGIKERTSFRLERRVA Sbjct: 61 LLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSDDNEGIKERTSFRLERRVA 120 Query: 121 AVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIAQTQTTNLAVVAPGIAEALLA 180 AVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIAQTQTTNLAVVAPGIAEALLA Sbjct: 121 AVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIAQTQTTNLAVVAPGIAEALLA 180 Query: 181 TAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLLLQSRDLDLEASAAAHPVRVAQK 240 TAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLLLQSRDLDLEASAAAHPVRVAQK Sbjct: 181 TAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLLLQSRDLDLEASAAAHPVRVAQK 240 Query: 241 LRAG 244 LRAG Sbjct: 241 LRAG 244 >5OCM-A nrPDB Length = 291 Score = 32.7 bits (73), Expect = 0.13, Method: Composition-based stats. Identities = 24/73 (32%), Positives = 33/73 (45%), Gaps = 12/73 (16%) Query: 124 RQMGRGNGYLATIGAISPF--VGLFGTVWGIMNSF------IGIAQTQTTNLAVVAPGIA 175 R +G G YL +S V L G +WG +NSF +G A+ + T A A Sbjct: 150 RSLGAGTTYLGADHGLSSLYDVALLGIMWGTLNSFLHGAALLGTAKVEATTFAPFANRWI 209 Query: 176 EALLATAIGLVAA 188 EA+ G V+A Sbjct: 210 EAV----TGFVSA 218 >5ZOV-A nrPDB Length = 465 Score = 31.6 bits (70), Expect = 0.37, Method: Composition-based stats. Identities = 20/77 (25%), Positives = 34/77 (44%), Gaps = 12/77 (15%) Query: 134 ATIGAISPFVGLFGTVWGIMNSFIGIAQTQT-TNLAVVAPGIAEALLATAIGLVAAIPAV 192 A I A SP +FG +WG + F+ + ++ ++ PG + A Sbjct: 316 AAIYAFSPNAMVFGFMWGAIGQFVAVGLLLGFSSPILIIPGFIPMFFSNA---------- 365 Query: 193 VIYNVFARQIGGFKAML 209 VFA Q GG+K+++ Sbjct: 366 -TIGVFANQFGGWKSVM 381 >4DBL-A nrPDB Length = 349 Score = 29.6 bits (65), Expect = 1.1, Method: Composition-based stats. Identities = 11/23 (47%), Positives = 19/23 (82%) Query: 26 VMIGLILASVVTWAIFFSKSVEF 48 V +G+I ++++TWAI+FS SV+ Sbjct: 173 VALGIISSALMTWAIYFSTSVDL 195 >1ZVD-A nrPDB Length = 380 Score = 29.3 bits (64), Expect = 1.5, Method: Composition-based stats. Identities = 17/64 (26%), Positives = 33/64 (51%), Gaps = 4/64 (6%) Query: 33 ASVVTWAIFFSKSVEFFNQKRRLKREQQLLAEAR-SLNQANDIAADFGSKSLSLHLLNEA 91 +++V W F K+VEFF+++RR + Q + +R L + G + ++H ++ Sbjct: 282 SNIVKW---FWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDAC 338 Query: 92 QNEL 95 N L Sbjct: 339 TNNL 342 >6FX4-A nrPDB Length = 122 Score = 28.9 bits (63), Expect = 2.1, Method: Composition-based stats. Identities = 17/64 (26%), Positives = 33/64 (51%), Gaps = 4/64 (6%) Query: 33 ASVVTWAIFFSKSVEFFNQKRRLKREQQLLAEAR-SLNQANDIAADFGSKSLSLHLLNEA 91 +++V W F K+VEFF+++RR + Q + +R L + G + ++H ++ Sbjct: 24 SNIVKW---FWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDAN 80 Query: 92 QNEL 95 N L Sbjct: 81 TNNL 84 >4FG0-A nrPDB Length = 407 Score = 28.1 bits (61), Expect = 3.4, Method: Composition-based stats. Identities = 21/66 (31%), Positives = 26/66 (39%), Gaps = 2/66 (3%) Query: 141 PFVGLFGTVWGIMNSFIGIAQTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFAR 200 P+ T W + + + T VVA G E L TA L AIPA V + Sbjct: 224 PWTSPATTDWRNRETLVEFEEPHATKQTVVALGSQEGALHTA--LAGAIPATVSSSTLTL 281 Query: 201 QIGGFK 206 Q G K Sbjct: 282 QSGHLK 287 >4U14-A nrPDB Length = 460 Score = 27.3 bits (59), Expect = 6.6, Method: Composition-based stats. Identities = 22/88 (25%), Positives = 37/88 (42%), Gaps = 5/88 (5%) Query: 15 MYQHADIVVKCVMIGLILASVVTWAIFFSKSVEFFNQKRRLKREQQLLAEARSLNQ---- 70 +Y D V +C +I ++ T F+ S+ QK R LA++R NQ Sbjct: 291 VYDSLDAVRRCALINMVFQMGETGVAGFTNSLRMLQQK-RWDEAAVNLAKSRWYNQCPNR 349 Query: 71 ANDIAADFGSKSLSLHLLNEAQNELELS 98 A + F + + +L+ E + LS Sbjct: 350 AKRVITTFRTGTWDAYLIKEKKAAQTLS 377 >3OP1-A nrPDB Length = 308 Score = 26.6 bits (57), Expect = 9.4, Method: Composition-based stats. Identities = 20/79 (25%), Positives = 34/79 (43%), Gaps = 5/79 (6%) Query: 47 EFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSDDNEG 106 E F + R+ L + N++ IA + L LH+LN A+ E +L EG Sbjct: 38 ELFRVANKAARKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKR-----EG 92 Query: 107 IKERTSFRLERRVAAVGRQ 125 ++E + A++ Q Sbjct: 93 VEELYLLDFSSQFASLTAQ 111 >4GAX-A nrPDB Length = 563 Score = 26.6 bits (57), Expect = 9.6, Method: Composition-based stats. Identities = 11/29 (37%), Positives = 15/29 (51%) Query: 123 GRQMGRGNGYLATIGAISPFVGLFGTVWG 151 G+QMGRG+ L I P ++WG Sbjct: 10 GQQMGRGSMSLTEEKPIRPIANFSPSIWG 38 >2ONJ-A nrPDB Length = 578 Score = 26.6 bits (57), Expect = 9.8, Method: Composition-based stats. Identities = 40/154 (25%), Positives = 65/154 (42%), Gaps = 26/154 (16%) Query: 28 IGLILASVVTWAIFFSKSVEFFNQKRRLKREQ-QLLAEARSL----NQANDIAADFGSKS 82 + L LA++ + + FF + R+L RE+ Q LAE + Q + F + Sbjct: 161 VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAIED 220 Query: 83 LSLHLLNEAQNELELSEGSDDNEGIKERT----SFRLERRVAAVGRQMGRGNG-YLA--- 134 NEA+N + + +K SF V +G + G G YLA Sbjct: 221 ------NEAKN-FDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISG 273 Query: 135 --TIGAISPFVG----LFGTVWGIMNSFIGIAQT 162 T+G ++ FVG LFG + ++ SF + Q+ Sbjct: 274 SITVGTLAAFVGYLELLFGPLRRLVASFTTLTQS 307 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.135 0.378 Lambda K H 0.267 0.0444 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,853,727 Number of Sequences: 36641 Number of extensions: 414489 Number of successful extensions: 1046 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 26 Number of HSP's that attempted gapping in prelim test: 1033 Number of HSP's gapped (non-prelim): 37 length of query: 244 length of database: 10,336,785 effective HSP length: 94 effective length of query: 150 effective length of database: 6,892,531 effective search space: 1033879650 effective search space used: 1033879650 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 58 (26.8 bits)