BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P09169 (317 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1I78-A nrPDB 604 e-173 4DCB-A nrPDB 282 1e-76 1L8K-A nrPDB 32 0.44 6ACN-A nrPDB 30 1.0 5XNL-4 nrPDB 29 2.2 4FDM-A nrPDB 28 5.5 2WTE-A nrPDB 28 5.6 6ELS-A nrPDB 28 6.9 3OPN-A nrPDB 27 10.0 >1I78-A nrPDB Length = 297 Score = 604 bits (1557), Expect = e-173, Method: Compositional matrix adjust. Identities = 294/297 (98%), Positives = 295/297 (99%) Query: 21 STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDL 80 STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDL Sbjct: 1 STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDL 60 Query: 81 MPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWL 140 MPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDE+RHPDTQLNYANEFDLNIKGWL Sbjct: 61 MPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDEARHPDTQLNYANEFDLNIKGWL 120 Query: 141 LNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPY 200 LNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPY Sbjct: 121 LNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPY 180 Query: 201 IGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYY 260 IGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDP RITYRSKVKDQNYYSVAVNAGYY Sbjct: 181 IGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPKGRITYRSKVKDQNYYSVAVNAGYY 240 Query: 261 VTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF 317 VTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF Sbjct: 241 VTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF 297 >4DCB-A nrPDB Length = 297 Score = 282 bits (722), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 148/292 (50%), Positives = 188/292 (64%), Gaps = 9/292 (3%) Query: 27 FTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISI 86 +PD+ S G LSGK+ E +Y AE G RK+SQLDWK N AI+KG I+WD +++ Sbjct: 5 ISPDSFTVAASTGMLSGKSHEMLYDAETG-RKISQLDWKIKNVAILKGDISWDPYSFLTL 63 Query: 87 GAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNY 146 A GWT+L S GNM + DWM+ N WTD S HP T +N+ANE+DLN+KGWLL + NY Sbjct: 64 NARGWTSLASGSGNMDNYDWMNE-NQSEWTDHSSHPATNVNHANEYDLNVKGWLLQDENY 122 Query: 147 RLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGS 206 + G+ AGYQE+R+S+TA GGSY Y++ G+FP G R IGY QRF MPYIGL G Sbjct: 123 KAGITAGYQETRFSWTATGGSYSYNNGA----YTGNFPKGVRVIGYNQRFSMPYIGLAGQ 178 Query: 207 YRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAK 266 YR DFEL FK+S WV + DNDEHY + +T+R K YY +NAGYYVTPNAK Sbjct: 179 YRINDFELNALFKFSDWVRAHDNDEHY--MRDLTFREKTSGSRYYGTVINAGYYVTPNAK 236 Query: 267 VYVEGAWNRVTNKKGNTSLYDHNNNTS-DYSKNGAGIENYNFITTAGLKYTF 317 V+ E +++ KG T D N+ S + AGI N N+ TAGL+Y F Sbjct: 237 VFAEFTYSKYDEGKGGTQTIDKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF 288 >1L8K-A nrPDB Length = 314 Score = 31.6 bits (70), Expect = 0.44, Method: Compositional matrix adjust. Identities = 14/31 (45%), Positives = 20/31 (64%) Query: 133 DLNIKGWLLNEPNYRLGLMAGYQESRYSFTA 163 D+NIK LLN YR+GL+ + R+S+ A Sbjct: 241 DINIKQVLLNMRKYRMGLIQTPDQLRFSYMA 271 >6ACN-A nrPDB Length = 754 Score = 30.4 bits (67), Expect = 1.0, Method: Compositional matrix adjust. Identities = 33/136 (24%), Positives = 52/136 (38%), Gaps = 10/136 (7%) Query: 179 DIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYD--PG 236 ++GS E G+ ++ IG + YED + PG Sbjct: 336 EVGSVAEKE---GWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPG 392 Query: 237 KRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKG--NTSLYDHNNNTSD 294 R+ ++ Y V + G V NA G W+R KKG NT + +N N + Sbjct: 393 SE-QIRATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFT- 450 Query: 295 YSKNGAGIENYNFITT 310 +N A E + F+T+ Sbjct: 451 -GRNDANPETHAFVTS 465 >5XNL-4 nrPDB Length = 210 Score = 29.3 bits (64), Expect = 2.2, Method: Compositional matrix adjust. Identities = 12/23 (52%), Positives = 15/23 (65%) Query: 91 WTTLGSRGGNMVDQDWMDSSNPG 113 W S GGN+VD +W+D S PG Sbjct: 9 WIPGVSFGGNLVDPEWLDGSLPG 31 >4FDM-A nrPDB Length = 394 Score = 28.1 bits (61), Expect = 5.5, Method: Compositional matrix adjust. Identities = 22/73 (30%), Positives = 33/73 (45%), Gaps = 2/73 (2%) Query: 186 GERAIGYKQRFKM-PYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSK 244 GE Y +R K P T + RY D + G K + WV++S N + TYR Sbjct: 224 GESFDHYFERLKRSPVWTSTDTARY-DLSVSGAEKLNQWVQASPNTYYLSFSTERTYRGA 282 Query: 245 VKDQNYYSVAVNA 257 + +Y + +NA Sbjct: 283 LTGNHYPELGMNA 295 >2WTE-A nrPDB Length = 244 Score = 28.1 bits (61), Expect = 5.6, Method: Compositional matrix adjust. Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 5/54 (9%) Query: 3 AKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGKTKERVYLAEEGG 56 +K+L I+LT P I S L+ IN I LG + + + VY+ +EGG Sbjct: 83 SKILDIILTLPEPIIS-----DLTMGMRMINTLILLGIIVSRKRFTVYVRDEGG 131 >6ELS-A nrPDB Length = 506 Score = 27.7 bits (60), Expect = 6.9, Method: Compositional matrix adjust. Identities = 9/19 (47%), Positives = 13/19 (68%) Query: 91 WTTLGSRGGNMVDQDWMDS 109 W TLG + ++ D DW+DS Sbjct: 284 WKTLGGKRTDLTDSDWLDS 302 >3OPN-A nrPDB Length = 232 Score = 27.3 bits (59), Expect = 10.0, Method: Compositional matrix adjust. Identities = 18/69 (26%), Positives = 33/69 (47%), Gaps = 9/69 (13%) Query: 110 SNPGTWTDESRHPDTQLNYANEFDLNIKGW---------LLNEPNYRLGLMAGYQESRYS 160 S+ G +TD +L YA + N W ++ + N+R ++A +++ R S Sbjct: 46 SSTGGFTDVMLQNGAKLVYALDVGTNQLAWKIRSDERVVVMEQFNFRNAVLADFEQGRPS 105 Query: 161 FTARGGSYI 169 FT+ S+I Sbjct: 106 FTSIDVSFI 114 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1I78-A nrPDB 523 e-149 4DCB-A nrPDB 443 e-125 Sequences not found previously or not previously below threshold: 3WN6-A nrPDB 34 0.074 1JI6-A nrPDB 33 0.18 3KVN-X nrPDB 32 0.25 1L8K-A nrPDB 31 0.77 4FDM-A nrPDB 31 0.84 4EPS-A nrPDB 30 1.1 6ACN-A nrPDB 30 1.8 3OKY-B nrPDB 29 3.5 CONVERGED! >1I78-A nrPDB Length = 297 Score = 523 bits (1346), Expect = e-149, Method: Composition-based stats. Identities = 294/297 (98%), Positives = 295/297 (99%) Query: 21 STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDL 80 STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDL Sbjct: 1 STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDL 60 Query: 81 MPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWL 140 MPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDE+RHPDTQLNYANEFDLNIKGWL Sbjct: 61 MPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDEARHPDTQLNYANEFDLNIKGWL 120 Query: 141 LNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPY 200 LNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPY Sbjct: 121 LNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPY 180 Query: 201 IGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYY 260 IGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDP RITYRSKVKDQNYYSVAVNAGYY Sbjct: 181 IGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPKGRITYRSKVKDQNYYSVAVNAGYY 240 Query: 261 VTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF 317 VTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF Sbjct: 241 VTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF 297 >4DCB-A nrPDB Length = 297 Score = 443 bits (1140), Expect = e-125, Method: Composition-based stats. Identities = 148/293 (50%), Positives = 189/293 (64%), Gaps = 9/293 (3%) Query: 26 SFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQIS 85 + +PD+ S G LSGK+ E +Y AE G RK+SQLDWK N AI+KG I+WD ++ Sbjct: 4 NISPDSFTVAASTGMLSGKSHEMLYDAETG-RKISQLDWKIKNVAILKGDISWDPYSFLT 62 Query: 86 IGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPN 145 + A GWT+L S GNM + DWM+ N WTD S HP T +N+ANE+DLN+KGWLL + N Sbjct: 63 LNARGWTSLASGSGNMDNYDWMNE-NQSEWTDHSSHPATNVNHANEYDLNVKGWLLQDEN 121 Query: 146 YRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTG 205 Y+ G+ AGYQE+R+S+TA GGSY Y++ G+FP G R IGY QRF MPYIGL G Sbjct: 122 YKAGITAGYQETRFSWTATGGSYSYNNGA----YTGNFPKGVRVIGYNQRFSMPYIGLAG 177 Query: 206 SYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNA 265 YR DFEL FK+S WV + DNDEHY + +T+R K YY +NAGYYVTPNA Sbjct: 178 QYRINDFELNALFKFSDWVRAHDNDEHY--MRDLTFREKTSGSRYYGTVINAGYYVTPNA 235 Query: 266 KVYVEGAWNRVTNKKGNTSLYDHNNNTS-DYSKNGAGIENYNFITTAGLKYTF 317 KV+ E +++ KG T D N+ S + AGI N N+ TAGL+Y F Sbjct: 236 KVFAEFTYSKYDEGKGGTQTIDKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF 288 >3WN6-A nrPDB Length = 404 Score = 34.3 bits (77), Expect = 0.074, Method: Composition-based stats. Identities = 22/121 (18%), Positives = 43/121 (35%), Gaps = 22/121 (18%) Query: 91 WTTLGSRGGNMVDQDWMDSSNPG--TWTDESRHPDTQLNYANEFDLNIKG---------- 138 WT++ + G + D ++ W D ++ A FD KG Sbjct: 206 WTSMANGGDGKPNYD-QNAHRQELVNWVDRVGGANSN---ATAFDFTTKGILNVAVEGEL 261 Query: 139 WLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKM 198 W L + + M G+ + A+ +++ + + G + FP+ + GY Sbjct: 262 WRLRGEDGKAPGMIGW------WPAKATTFVDNHDTGSTQHLWPFPSDKVMQGYAYILTH 315 Query: 199 P 199 P Sbjct: 316 P 316 >1JI6-A nrPDB Length = 589 Score = 33.1 bits (74), Expect = 0.18, Method: Composition-based stats. Identities = 16/72 (22%), Positives = 30/72 (41%), Gaps = 9/72 (12%) Query: 230 DEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGA---WNRVTNKKG--NTS 284 D+ +P +++++ Q Y N PN KVY+ +++ ++K +T Sbjct: 321 DKSTEPVQKLSF----DGQKVYRTIANTDVAAWPNGKVYLGVTKVDFSQYDDQKNETSTQ 376 Query: 285 LYDHNNNTSDYS 296 YD N S Sbjct: 377 TYDSKRNNGHVS 388 >3KVN-X nrPDB Length = 632 Score = 32.4 bits (72), Expect = 0.25, Method: Composition-based stats. Identities = 46/224 (20%), Positives = 82/224 (36%), Gaps = 40/224 (17%) Query: 87 GAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNY 146 W GG +D D DS+ G N ++L + G + + Sbjct: 353 NVGQWRGFVGGGGQRLDFDSQDSAASGD--------------GNGYNLTLGGSYRIDEAW 398 Query: 147 RLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGS 206 R G+ AG+ + A+ Y +S + S + Y++ LTG Sbjct: 399 RAGVAAGFYRQKLEAGAKDSDYRMNS------YMAS-----AFVQYQENRWWADAALTGG 447 Query: 207 YRYEDFELGGTFKYSGWVESSDNDEH---YDPGKRITYR-SKVKDQNYY-SVAVNAGYYV 261 Y D +L F G S D + + R+ Y ++ D ++ S V+A Y Sbjct: 448 YLDYD-DLKRKFALGGGERSEKGDTNGHLWAFSARLGYDIAQQADSPWHLSPFVSADY-- 504 Query: 262 TPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENY 305 A+V V+G + K + + D+++ + GAG++ Sbjct: 505 ---ARVEVDG----YSEKGASATALDYDDQKRSSKRLGAGLQGK 541 >1L8K-A nrPDB Length = 314 Score = 30.8 bits (68), Expect = 0.77, Method: Composition-based stats. Identities = 14/31 (45%), Positives = 20/31 (64%) Query: 133 DLNIKGWLLNEPNYRLGLMAGYQESRYSFTA 163 D+NIK LLN YR+GL+ + R+S+ A Sbjct: 241 DINIKQVLLNMRKYRMGLIQTPDQLRFSYMA 271 >4FDM-A nrPDB Length = 394 Score = 30.8 bits (68), Expect = 0.84, Method: Composition-based stats. Identities = 22/73 (30%), Positives = 33/73 (45%), Gaps = 2/73 (2%) Query: 186 GERAIGYKQRFKM-PYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSK 244 GE Y +R K P T + RY D + G K + WV++S N + TYR Sbjct: 224 GESFDHYFERLKRSPVWTSTDTARY-DLSVSGAEKLNQWVQASPNTYYLSFSTERTYRGA 282 Query: 245 VKDQNYYSVAVNA 257 + +Y + +NA Sbjct: 283 LTGNHYPELGMNA 295 >4EPS-A nrPDB Length = 510 Score = 30.4 bits (67), Expect = 1.1, Method: Composition-based stats. Identities = 23/105 (21%), Positives = 43/105 (40%), Gaps = 5/105 (4%) Query: 196 FKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKR----ITYRSKVKDQNYY 251 F Y+ +TG Y + ++ G+ S D +YD K Y+ + ++ Sbjct: 363 FTSSYVSVTGYYDANENNKNKPLRFLGYNNSEVLDLYYDTFKTDFECFQYQDDC-ESSFP 421 Query: 252 SVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYS 296 S ++ G+YV + ++ + K NT L ++ T D S Sbjct: 422 SPSITTGWYVPSSGELVALQDKDNSLESKLNTKLIKVSDKTMDIS 466 >6ACN-A nrPDB Length = 754 Score = 29.7 bits (65), Expect = 1.8, Method: Composition-based stats. Identities = 19/69 (27%), Positives = 31/69 (44%) Query: 242 RSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAG 301 R+ ++ Y V + G V NA G W+R KKG + + N + +N A Sbjct: 397 RATIERDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDAN 456 Query: 302 IENYNFITT 310 E + F+T+ Sbjct: 457 PETHAFVTS 465 >3OKY-B nrPDB Length = 565 Score = 28.5 bits (62), Expect = 3.5, Method: Composition-based stats. Identities = 10/30 (33%), Positives = 15/30 (50%) Query: 220 YSGWVESSDNDEHYDPGKRITYRSKVKDQN 249 Y GWV S + H P R+T+ ++ N Sbjct: 517 YCGWVRESGSCAHLSPLSRLTFEQDIERGN 546 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.308 0.132 0.389 Lambda K H 0.267 0.0404 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,521,578 Number of Sequences: 36641 Number of extensions: 597587 Number of successful extensions: 1098 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 18 Number of HSP's that attempted gapping in prelim test: 1068 Number of HSP's gapped (non-prelim): 35 length of query: 317 length of database: 10,336,785 effective HSP length: 97 effective length of query: 220 effective length of database: 6,782,608 effective search space: 1492173760 effective search space used: 1492173760 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 59 (27.4 bits)