BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P06149 (571 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1F0X-A nrPDB 1192 0.0 3PM9-A nrPDB 46 4e-05 1H76-A nrPDB 31 1.3 >1F0X-A nrPDB Length = 571 Score = 1192 bits (3084), Expect = 0.0, Method: Compositional matrix adjust. Identities = 571/571 (100%), Positives = 571/571 (100%) Query: 1 MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQGDALAVVFPGSLLELWR 60 MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQGDALAVVFPGSLLELWR Sbjct: 1 MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQGDALAVVFPGSLLELWR 60 Query: 61 VLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVLGKGEQVLAYP 120 VLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVLGKGEQVLAYP Sbjct: 61 VLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVLGKGEQVLAYP 120 Query: 121 GTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSGGSLVQRGPAYTEMSLFARIN 180 GTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSGGSLVQRGPAYTEMSLFARIN Sbjct: 121 GTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSGGSLVQRGPAYTEMSLFARIN 180 Query: 181 EDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRHDGRHAHDYDYVHRVRDIEADTP 240 EDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRHDGRHAHDYDYVHRVRDIEADTP Sbjct: 181 EDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRHDGRHAHDYDYVHRVRDIEADTP 240 Query: 241 ARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRHILANF 300 ARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRHILANF Sbjct: 241 ARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRHILANF 300 Query: 301 ENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEKVKFFRPH 360 ENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEKVKFFRPH Sbjct: 301 ENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEKVKFFRPH 360 Query: 361 FTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDYFKQAEGDFF 420 FTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDYFKQAEGDFF Sbjct: 361 FTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDYFKQAEGDFF 420 Query: 421 VCTPEEGSKAFLHRFAAAGAAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPPEIDSQ 480 VCTPEEGSKAFLHRFAAAGAAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPPEIDSQ Sbjct: 421 VCTPEEGSKAFLHRFAAAGAAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPPEIDSQ 480 Query: 481 LVHKLYYGHFMCYVFHQDYIVKKGVDVHALKEQMLELLQQRGAQYPAEHNVGHLYKAPET 540 LVHKLYYGHFMCYVFHQDYIVKKGVDVHALKEQMLELLQQRGAQYPAEHNVGHLYKAPET Sbjct: 481 LVHKLYYGHFMCYVFHQDYIVKKGVDVHALKEQMLELLQQRGAQYPAEHNVGHLYKAPET 540 Query: 541 LQKFYRENDPTNSMNPGIGKTSKRKNWQEVE 571 LQKFYRENDPTNSMNPGIGKTSKRKNWQEVE Sbjct: 541 LQKFYRENDPTNSMNPGIGKTSKRKNWQEVE 571 >3PM9-A nrPDB Length = 476 Score = 45.8 bits (107), Expect = 4e-05, Method: Compositional matrix adjust. Identities = 43/160 (26%), Positives = 72/160 (45%), Gaps = 10/160 (6%) Query: 6 TTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSG-QGDALAVVFPGSLLELWRVLKA 64 T + + +VG H LTDP + Y R+ +G + V+ PGS E+ + K Sbjct: 11 VTLSPELIARFTAIVGDKHALTDPLELEAYITEERNLYRGHSPLVLRPGSTEEVVAICKL 70 Query: 65 CVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVLGKGEQVLAY-PGTT 123 A ++ Q NTGL G TP+ + V+IS R+DK+ + + G Sbjct: 71 ANEARVALVPQGGNTGLVGGQTPHNGE-----VVISLKRMDKIREIDTSSNTITVEAGAI 125 Query: 124 LYSLEKALKPLGREPHSVIGSSCIGASVIGG-ICNNSGGS 162 L +++ + R +G+ G+ IGG + N+GG+ Sbjct: 126 LQRVQEKAAEVDRLFPLSLGAQ--GSCTIGGNLSTNAGGT 163 >1H76-A nrPDB Length = 696 Score = 31.2 bits (69), Expect = 1.3, Method: Compositional matrix adjust. Identities = 24/73 (32%), Positives = 35/73 (47%), Gaps = 17/73 (23%) Query: 483 HKLYYGH---FMCYV------FHQDYIVKKGVD-------VHALKEQMLELLQQRGAQYP 526 H+ YYG+ F C V F +D +V++ D LK+ ELL Q GA+ P Sbjct: 520 HERYYGYTGAFRCLVEKGDVAFVKDQVVQQNTDGKNKDDWAKDLKQMDFELLCQNGAREP 579 Query: 527 AEH-NVGHLYKAP 538 ++ HL +AP Sbjct: 580 VDNAENCHLARAP 592 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1F0X-A nrPDB 1162 0.0 3PM9-A nrPDB 218 6e-57 Sequences not found previously or not previously below threshold: 5FXF-A nrPDB 44 2e-04 2UUU-A nrPDB 44 2e-04 4BC9-A nrPDB 33 0.27 1H76-A nrPDB 32 1.1 6CLW-A nrPDB 30 2.3 3UCA-A nrPDB 30 2.4 5UCM-A nrPDB 29 4.6 1DIP-A nrPDB 29 6.4 4N9F-b nrPDB 28 9.1 >1F0X-A nrPDB Length = 571 Score = 1162 bits (3006), Expect = 0.0, Method: Composition-based stats. Identities = 571/571 (100%), Positives = 571/571 (100%) Query: 1 MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQGDALAVVFPGSLLELWR 60 MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQGDALAVVFPGSLLELWR Sbjct: 1 MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQGDALAVVFPGSLLELWR 60 Query: 61 VLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVLGKGEQVLAYP 120 VLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVLGKGEQVLAYP Sbjct: 61 VLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVLGKGEQVLAYP 120 Query: 121 GTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSGGSLVQRGPAYTEMSLFARIN 180 GTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSGGSLVQRGPAYTEMSLFARIN Sbjct: 121 GTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSGGSLVQRGPAYTEMSLFARIN 180 Query: 181 EDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRHDGRHAHDYDYVHRVRDIEADTP 240 EDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRHDGRHAHDYDYVHRVRDIEADTP Sbjct: 181 EDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRHDGRHAHDYDYVHRVRDIEADTP 240 Query: 241 ARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRHILANF 300 ARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRHILANF Sbjct: 241 ARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRHILANF 300 Query: 301 ENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEKVKFFRPH 360 ENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEKVKFFRPH Sbjct: 301 ENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEKVKFFRPH 360 Query: 361 FTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDYFKQAEGDFF 420 FTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDYFKQAEGDFF Sbjct: 361 FTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDYFKQAEGDFF 420 Query: 421 VCTPEEGSKAFLHRFAAAGAAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPPEIDSQ 480 VCTPEEGSKAFLHRFAAAGAAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPPEIDSQ Sbjct: 421 VCTPEEGSKAFLHRFAAAGAAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPPEIDSQ 480 Query: 481 LVHKLYYGHFMCYVFHQDYIVKKGVDVHALKEQMLELLQQRGAQYPAEHNVGHLYKAPET 540 LVHKLYYGHFMCYVFHQDYIVKKGVDVHALKEQMLELLQQRGAQYPAEHNVGHLYKAPET Sbjct: 481 LVHKLYYGHFMCYVFHQDYIVKKGVDVHALKEQMLELLQQRGAQYPAEHNVGHLYKAPET 540 Query: 541 LQKFYRENDPTNSMNPGIGKTSKRKNWQEVE 571 LQKFYRENDPTNSMNPGIGKTSKRKNWQEVE Sbjct: 541 LQKFYRENDPTNSMNPGIGKTSKRKNWQEVE 571 >3PM9-A nrPDB Length = 476 Score = 218 bits (555), Expect = 6e-57, Method: Composition-based stats. Identities = 43/160 (26%), Positives = 72/160 (45%), Gaps = 10/160 (6%) Query: 6 TTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSG-QGDALAVVFPGSLLELWRVLKA 64 T + + +VG H LTDP + Y R+ +G + V+ PGS E+ + K Sbjct: 11 VTLSPELIARFTAIVGDKHALTDPLELEAYITEERNLYRGHSPLVLRPGSTEEVVAICKL 70 Query: 65 CVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVLGKGEQVLAY-PGTT 123 A ++ Q NTGL G TP+ + V+IS R+DK+ + + G Sbjct: 71 ANEARVALVPQGGNTGLVGGQTPHNGE-----VVISLKRMDKIREIDTSSNTITVEAGAI 125 Query: 124 LYSLEKALKPLGREPHSVIGSSCIGASVIGG-ICNNSGGS 162 L +++ + R +G+ G+ IGG + N+GG+ Sbjct: 126 LQRVQEKAAEVDRLFPLSLGAQ--GSCTIGGNLSTNAGGT 163 >5FXF-A nrPDB Length = 526 Score = 44.3 bits (103), Expect = 2e-04, Method: Composition-based stats. Identities = 36/182 (19%), Positives = 64/182 (35%), Gaps = 27/182 (14%) Query: 4 MTTTDNK--------AFLNELARLVGSSHLLTDPAKTARYRKGFRSGQGDA---LAVVFP 52 MT T A L +VG +L+ + +R + G +A AVV P Sbjct: 1 MTRTLPPGVSDERFDAALQRFRDVVGDKWVLSTADELEAFRDPYPVGAAEANLPSAVVSP 60 Query: 53 GSLLELWRVLKACVTADKIILM--QAANTGLTEGSTPNGNDYDRDVVIISTLRLDKLHVL 110 S ++ +++ + N G G+ P V++ + R++++ + Sbjct: 61 ESTEQVQDIVRIANEYGIPLSPVSTGKNNGY-GGAAPR---LSGSVIVKTGERMNRILEV 116 Query: 111 G-KGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSGGSLVQRGPA 169 K L PG T + L + L+ G+ V G+ + RG Sbjct: 117 NEKYGYALLEPGVTYFDLYEYLQSHDSGLMLDCPDLGWGSVV---------GNTLDRGVG 167 Query: 170 YT 171 YT Sbjct: 168 YT 169 >2UUU-A nrPDB Length = 584 Score = 43.9 bits (102), Expect = 2e-04, Method: Composition-based stats. Identities = 31/149 (20%), Positives = 56/149 (37%), Gaps = 15/149 (10%) Query: 48 AVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTLRLDKL 107 +V P S E+ R+++ + +I+ + + P N+ R V I R++K+ Sbjct: 140 LIVLPHSHEEVERLVQLAHKYNVVIIPMGGGSNIVGAIEPVSNE--RFTVSIDMRRMNKV 197 Query: 108 HVLGKGEQVLAYPGTTL-----YSLEKALKPLGREPHSVIGSSCIGASVIGG-ICNNSGG 161 + + E + L K LG +P S S +GG + S G Sbjct: 198 LWVDRREMTACIQVGIMGPELEKQLHKQGVSLGHDPDS------FEFSTLGGWLATCSSG 251 Query: 162 SLVQRGPAYTEMSL-FARINEDGKLTLVN 189 + +M++ F + G L L N Sbjct: 252 HQSDKYGDIEDMAVSFRTVTPTGTLELRN 280 >4BC9-A nrPDB Length = 658 Score = 33.5 bits (75), Expect = 0.27, Method: Composition-based stats. Identities = 24/121 (19%), Positives = 49/121 (40%), Gaps = 15/121 (12%) Query: 48 AVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEG-STPNGNDYDRDVVIISTLRLDK 106 V++P ++ +++ + I+ T ++ G P D R ++ + T ++++ Sbjct: 208 IVLWPTCHDDVVKIVNLACKYNLCIIPIGGGTSVSYGLMCP--ADETRTIISLDTSQMNR 265 Query: 107 LHVLGKGEQVLAY-PGTTLYSLEKALKP----LGREPHSVIGSSCIGASVIGG-ICNNSG 160 + + + G T LE+ LK G EP S + S +GG I + Sbjct: 266 ILWVDENNLTAHVEAGITGQELERQLKESGYCTGHEPDS------LEFSTVGGWISTRAS 319 Query: 161 G 161 G Sbjct: 320 G 320 >1H76-A nrPDB Length = 696 Score = 31.5 bits (70), Expect = 1.1, Method: Composition-based stats. Identities = 24/73 (32%), Positives = 35/73 (47%), Gaps = 17/73 (23%) Query: 483 HKLYYGH---FMCYV------FHQDYIVKKGVD-------VHALKEQMLELLQQRGAQYP 526 H+ YYG+ F C V F +D +V++ D LK+ ELL Q GA+ P Sbjct: 520 HERYYGYTGAFRCLVEKGDVAFVKDQVVQQNTDGKNKDDWAKDLKQMDFELLCQNGAREP 579 Query: 527 AEHNVG-HLYKAP 538 ++ HL +AP Sbjct: 580 VDNAENCHLARAP 592 >6CLW-A nrPDB Length = 364 Score = 30.4 bits (67), Expect = 2.3, Method: Composition-based stats. Identities = 20/76 (26%), Positives = 24/76 (31%), Gaps = 1/76 (1%) Query: 108 HVLGKGEQVLAYPGTTLY-SLEKALKPLGREPHSVIGSSCIGASVIGGICNNSGGSLVQR 166 HV+ G L L R P G GAS G C SGGS Sbjct: 192 HVMDVGGGTGGVLAEVLRARPGLKGTLLDRAPTVAAGREAWGASEAGQRCTFSGGSFFDT 251 Query: 167 GPAYTEMSLFARINED 182 P+ + L + D Sbjct: 252 LPSGADACLLVNVLHD 267 >3UCA-A nrPDB Length = 324 Score = 30.4 bits (67), Expect = 2.4, Method: Composition-based stats. Identities = 27/97 (27%), Positives = 45/97 (46%), Gaps = 13/97 (13%) Query: 44 GDALAVVFPGSLL-ELWRVL--------KACVTADKIILMQAANTGLTEGSTPNGNDYDR 94 G+A+AV+ +LL E ++L K+ + A KII A + G+ G + + D+ Sbjct: 136 GEAIAVLAGDALLNEAMKILVDYSLEEGKSALKATKIIADAAGSDGMIGGQIVDIINEDK 195 Query: 95 DVVIISTLRLDKLHVLGKGEQVLA--YPGTTLYSLEK 129 + IS LD +H+ GE + A G L + Sbjct: 196 EE--ISLKELDYMHLKKTGELIKASIMSGAVLAEASE 230 >5UCM-A nrPDB Length = 579 Score = 29.2 bits (64), Expect = 4.6, Method: Composition-based stats. Identities = 26/122 (21%), Positives = 54/122 (44%), Gaps = 25/122 (20%) Query: 247 PDRLFESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRHILANFENLPVA 306 P L++ SG + +RL +++++ F +G EV+T++ R+ L +++ LP+ Sbjct: 84 PAELWQESGRWEQYGPELLRLK----DRHEREFCVGPTHEEVITDLARNELNSYKQLPI- 138 Query: 307 GEYMHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAM 366 + Y I K+ + P F ++GR M + F H + ++ Sbjct: 139 ------NFYQIQTKFRDEI-----------RPRFGLMRGREFIMKDAYSF---HLSQDSL 178 Query: 367 QK 368 Q+ Sbjct: 179 QQ 180 >1DIP-A nrPDB Length = 78 Score = 28.9 bits (63), Expect = 6.4, Method: Composition-based stats. Identities = 17/49 (34%), Positives = 30/49 (61%), Gaps = 3/49 (6%) Query: 496 HQDYIVKKGVDVHALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKF 544 H Y V++ V++ LKEQ+ EL++ + +Q E+ + +PE L+KF Sbjct: 8 HLMYAVREEVEI--LKEQIRELVE-KNSQLERENTLLKTLASPEQLEKF 53 >4N9F-b nrPDB Length = 176 Score = 28.5 bits (62), Expect = 9.1, Method: Composition-based stats. Identities = 20/111 (18%), Positives = 44/111 (39%), Gaps = 12/111 (10%) Query: 380 RMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAG 439 R+ W+ +HH+ + +AK W + ++ P+ S+ + A Sbjct: 17 RINTWKRLVKHHMYISR------KAKDWFYRHHYEST------NPKISSEVHIPLGDAKL 64 Query: 440 AAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHF 490 Y +H+ E + L +++ + + P++ QL+H Y+ F Sbjct: 65 VITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDLADQLIHLHYFDCF 115 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.136 0.397 Lambda K H 0.267 0.0418 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 24,925,845 Number of Sequences: 36641 Number of extensions: 1116355 Number of successful extensions: 2625 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 2614 Number of HSP's gapped (non-prelim): 21 length of query: 571 length of database: 10,336,785 effective HSP length: 102 effective length of query: 469 effective length of database: 6,599,403 effective search space: 3095120007 effective search space used: 3095120007 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 62 (28.5 bits)