BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P06100 (191 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4BY6-B nrPDB 398 e-112 5FU6-B nrPDB 88 2e-18 4C0F-A nrPDB 75 2e-14 4C0G-A nrPDB 52 1e-07 4C0D-C nrPDB 52 2e-07 5FU6-C nrPDB 52 2e-07 4BY6-C nrPDB 45 1e-05 2RFQ-A nrPDB 29 1.4 2WYA-A nrPDB 27 6.7 3G7U-A nrPDB 27 7.4 >4BY6-B nrPDB Length = 191 Score = 398 bits (1023), Expect = e-112, Method: Compositional matrix adjust. Identities = 191/191 (100%), Positives = 191/191 (100%) Query: 1 MEKFGLKALVPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPW 60 MEKFGLKALVPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPW Sbjct: 1 MEKFGLKALVPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPW 60 Query: 61 AETSRSEVEPRFFTPESFTNIPGVLQSTVTPPCFNSIQNDQQRVALFQDETLFFLFYKHP 120 AETSRSEVEPRFFTPESFTNIPGVLQSTVTPPCFNSIQNDQQRVALFQDETLFFLFYKHP Sbjct: 61 AETSRSEVEPRFFTPESFTNIPGVLQSTVTPPCFNSIQNDQQRVALFQDETLFFLFYKHP 120 Query: 121 GTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQ 180 GTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQ Sbjct: 121 GTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQ 180 Query: 181 RDFLLFYNAIM 191 RDFLLFYNAIM Sbjct: 181 RDFLLFYNAIM 191 >5FU6-B nrPDB Length = 197 Score = 88.2 bits (217), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 56/192 (29%), Positives = 97/192 (50%), Gaps = 26/192 (13%) Query: 2 EKFGLKALVPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPWA 61 ++FG+ L+ ++ + + + + LG+DL+++ +L P + + F SPWA Sbjct: 10 DQFGMIGLLTFIRAAETDPGMVH---LALGSDLTTLGLNLNSPEN-----LYPKFASPWA 61 Query: 62 ETSRSEVEPRFFTP-ESFTNIPGVLQSTVTPPCFNSIQNDQQRVAL--FQDETLFFLFYK 118 + + F P E TNI I++ + L + ++ LF+L+Y Sbjct: 62 SSPCRPQDIDFHVPSEYLTNI--------------HIRDKLAAIKLGRYGEDLLFYLYYM 107 Query: 119 HPGTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEK 178 + G V+Q L +EL R+WRYHK + W+T+ P MEP + + ERG+Y FFD W K Sbjct: 108 NGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTY-ERGTYYFFDCLNWRK 166 Query: 179 CQRDFLLFYNAI 190 ++F L Y+ + Sbjct: 167 VAKEFHLEYDKL 178 >4C0F-A nrPDB Length = 117 Score = 75.1 bits (183), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 34/88 (38%), Positives = 55/88 (62%), Gaps = 1/88 (1%) Query: 103 RVALFQDETLFFLFYKHPGTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGL 162 ++ + ++ LF+L+Y + G V+Q L +EL R+WRYHK + W+T+ P MEP + + Sbjct: 12 KLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTN-T 70 Query: 163 SERGSYVFFDPQRWEKCQRDFLLFYNAI 190 ERG+Y FFD W K ++F L Y+ + Sbjct: 71 YERGTYYFFDCLNWRKVAKEFHLEYDKL 98 >4C0G-A nrPDB Length = 102 Score = 52.4 bits (124), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 27/77 (35%), Positives = 43/77 (55%), Gaps = 3/77 (3%) Query: 110 ETLFFLFYKHPGTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYV 169 ETLFF+FY GT Q L L+K++WR+H W + EP D E+G+Y+ Sbjct: 18 ETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHE--EPKTITDEF-EQGTYI 74 Query: 170 FFDPQRWEKCQRDFLLF 186 +FD ++W + +++ F Sbjct: 75 YFDYEKWGQRKKEGFTF 91 >4C0D-C nrPDB Length = 166 Score = 51.6 bits (122), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 31/96 (32%), Positives = 50/96 (52%), Gaps = 6/96 (6%) Query: 91 PPCFNSIQNDQQRVALFQDETLFFLFYKHPGTVIQELTYLELRKRNWRYHKTLKAWLTKD 150 PP + QR++ ETLFF+FY GT Q L L+K++WR+H W + Sbjct: 66 PPPHSDTVEFYQRLST---ETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRH 122 Query: 151 PMMEPIVSADGLSERGSYVFFDPQRWEKCQRDFLLF 186 EP D E+G+Y++FD ++W + +++ F Sbjct: 123 E--EPKTITDEF-EQGTYIYFDYEKWGQRKKEGFTF 155 >5FU6-C nrPDB Length = 148 Score = 51.6 bits (122), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 31/96 (32%), Positives = 50/96 (52%), Gaps = 6/96 (6%) Query: 91 PPCFNSIQNDQQRVALFQDETLFFLFYKHPGTVIQELTYLELRKRNWRYHKTLKAWLTKD 150 PP + QR++ ETLFF+FY GT Q L L+K++WR+H W + Sbjct: 53 PPPHSDTVEFYQRLS---TETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRH 109 Query: 151 PMMEPIVSADGLSERGSYVFFDPQRWEKCQRDFLLF 186 EP D E+G+Y++FD ++W + +++ F Sbjct: 110 --EEPKTITDEF-EQGTYIYFDYEKWGQRKKEGFTF 142 >4BY6-C nrPDB Length = 262 Score = 45.4 bits (106), Expect = 1e-05, Method: Compositional matrix adjust. Identities = 29/82 (35%), Positives = 46/82 (56%), Gaps = 6/82 (7%) Query: 107 FQDETLFFLFYKHPGTVIQELTYLEL-RKRNWRYHKTLKAWLTKDPMMEPIVSADGLSER 165 F +TLFF+FY + G+ Q L EL + RNW ++K + W K+ +E + G SE Sbjct: 174 FDLDTLFFIFYHYQGSYEQFLAARELFKNRNWLFNKVDRCWYYKE--IEKLPPGMGKSEE 231 Query: 166 GSYVFFDPQR-W--EKCQRDFL 184 S+ +FD ++ W +C DF+ Sbjct: 232 ESWRYFDYKKSWLARRCGNDFV 253 >2RFQ-A nrPDB Length = 394 Score = 28.9 bits (63), Expect = 1.4, Method: Compositional matrix adjust. Identities = 16/47 (34%), Positives = 24/47 (51%) Query: 144 KAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQRDFLLFYNAI 190 +A T+D P S L E G + P++W Q D +LFY+A+ Sbjct: 26 RAQETEDLRRIPDDSMKALQETGFFRLLQPEQWGGYQADPVLFYSAV 72 >2WYA-A nrPDB Length = 460 Score = 26.6 bits (57), Expect = 6.7, Method: Compositional matrix adjust. Identities = 14/64 (21%), Positives = 31/64 (48%) Query: 18 KELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPWAETSRSEVEPRFFTPES 77 +EL+ + + + G+ L++ +S + +D+ LD S ++ + + +PE Sbjct: 352 QELAGSRIGAFSYGSGLAASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLASRKCVSPEE 411 Query: 78 FTNI 81 FT I Sbjct: 412 FTEI 415 >3G7U-A nrPDB Length = 376 Score = 26.6 bits (57), Expect = 7.4, Method: Compositional matrix adjust. Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 6/38 (15%) Query: 66 SEVEPRFFTPESFTNIPGVLQ---STVTPPCFNSIQND 100 SE++P FF E N+PG++Q S + FN + D Sbjct: 108 SELQPLFFLAE---NVPGIMQEKYSGIRNKAFNLVSGD 142 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4BY6-B nrPDB 297 2e-81 5FU6-B nrPDB 239 7e-64 4C0F-A nrPDB 134 2e-32 5FU6-C nrPDB 119 9e-28 4C0D-C nrPDB 119 1e-27 4C0G-A nrPDB 112 6e-26 4BY6-C nrPDB 100 5e-22 Sequences not found previously or not previously below threshold: 1T7N-A nrPDB 29 1.00 6B18-A nrPDB 29 1.2 2WYA-A nrPDB 28 2.3 3DKQ-A nrPDB 27 3.5 3ZKC-A nrPDB 27 6.5 CONVERGED! >4BY6-B nrPDB Length = 191 Score = 297 bits (760), Expect = 2e-81, Method: Composition-based stats. Identities = 191/191 (100%), Positives = 191/191 (100%) Query: 1 MEKFGLKALVPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPW 60 MEKFGLKALVPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPW Sbjct: 1 MEKFGLKALVPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPW 60 Query: 61 AETSRSEVEPRFFTPESFTNIPGVLQSTVTPPCFNSIQNDQQRVALFQDETLFFLFYKHP 120 AETSRSEVEPRFFTPESFTNIPGVLQSTVTPPCFNSIQNDQQRVALFQDETLFFLFYKHP Sbjct: 61 AETSRSEVEPRFFTPESFTNIPGVLQSTVTPPCFNSIQNDQQRVALFQDETLFFLFYKHP 120 Query: 121 GTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQ 180 GTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQ Sbjct: 121 GTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQ 180 Query: 181 RDFLLFYNAIM 191 RDFLLFYNAIM Sbjct: 181 RDFLLFYNAIM 191 >5FU6-B nrPDB Length = 197 Score = 239 bits (609), Expect = 7e-64, Method: Composition-based stats. Identities = 55/192 (28%), Positives = 97/192 (50%), Gaps = 26/192 (13%) Query: 2 EKFGLKALVPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPWA 61 ++FG+ L+ ++ + + + + LG+DL+++ +L P + + F SPWA Sbjct: 10 DQFGMIGLLTFIRAAETDPGMVH---LALGSDLTTLGLNLNSPEN-----LYPKFASPWA 61 Query: 62 ETSRSEVEPRFFTP-ESFTNIPGVLQSTVTPPCFNSIQNDQQ--RVALFQDETLFFLFYK 118 + + F P E TNI I++ ++ + ++ LF+L+Y Sbjct: 62 SSPCRPQDIDFHVPSEYLTNI--------------HIRDKLAAIKLGRYGEDLLFYLYYM 107 Query: 119 HPGTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEK 178 + G V+Q L +EL R+WRYHK + W+T+ P MEP + + ERG+Y FFD W K Sbjct: 108 NGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTY-ERGTYYFFDCLNWRK 166 Query: 179 CQRDFLLFYNAI 190 ++F L Y+ + Sbjct: 167 VAKEFHLEYDKL 178 >4C0F-A nrPDB Length = 117 Score = 134 bits (338), Expect = 2e-32, Method: Composition-based stats. Identities = 34/88 (38%), Positives = 55/88 (62%), Gaps = 1/88 (1%) Query: 103 RVALFQDETLFFLFYKHPGTVIQELTYLELRKRNWRYHKTLKAWLTKDPMMEPIVSADGL 162 ++ + ++ LF+L+Y + G V+Q L +EL R+WRYHK + W+T+ P MEP + + Sbjct: 12 KLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTY 71 Query: 163 SERGSYVFFDPQRWEKCQRDFLLFYNAI 190 ERG+Y FFD W K ++F L Y+ + Sbjct: 72 -ERGTYYFFDCLNWRKVAKEFHLEYDKL 98 >5FU6-C nrPDB Length = 148 Score = 119 bits (297), Expect = 9e-28, Method: Composition-based stats. Identities = 32/105 (30%), Positives = 51/105 (48%), Gaps = 6/105 (5%) Query: 82 PGVLQSTVTPPCFNSIQNDQQRVALFQDETLFFLFYKHPGTVIQELTYLELRKRNWRYHK 141 P PP + QR++ ETLFF+FY GT Q L L+K++WR+H Sbjct: 44 PTPPYHHQMPPPHSDTVEFYQRLST---ETLFFIFYYLEGTKAQYLAAKALKKQSWRFHT 100 Query: 142 TLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQRDFLLF 186 W + EP D E+G+Y++FD ++W + +++ F Sbjct: 101 KYMMWFQRHE--EPKTITDEF-EQGTYIYFDYEKWGQRKKEGFTF 142 >4C0D-C nrPDB Length = 166 Score = 119 bits (297), Expect = 1e-27, Method: Composition-based stats. Identities = 32/105 (30%), Positives = 51/105 (48%), Gaps = 6/105 (5%) Query: 82 PGVLQSTVTPPCFNSIQNDQQRVALFQDETLFFLFYKHPGTVIQELTYLELRKRNWRYHK 141 P PP + QR++ ETLFF+FY GT Q L L+K++WR+H Sbjct: 57 PTPPYHHQMPPPHSDTVEFYQRLST---ETLFFIFYYLEGTKAQYLAAKALKKQSWRFHT 113 Query: 142 TLKAWLTKDPMMEPIVSADGLSERGSYVFFDPQRWEKCQRDFLLF 186 W + EP D E+G+Y++FD ++W + +++ F Sbjct: 114 KYMMWFQRHE--EPKTITDEF-EQGTYIYFDYEKWGQRKKEGFTF 155 >4C0G-A nrPDB Length = 102 Score = 112 bits (281), Expect = 6e-26, Method: Composition-based stats. Identities = 29/93 (31%), Positives = 48/93 (51%), Gaps = 6/93 (6%) Query: 94 FNSIQNDQQRVALFQDETLFFLFYKHPGTVIQELTYLELRKRNWRYHKTLKAWLTKDPMM 153 + QR++ ETLFF+FY GT Q L L+K++WR+H W + Sbjct: 5 HSDTVEFYQRLST---ETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHE-- 59 Query: 154 EPIVSADGLSERGSYVFFDPQRWEKCQRDFLLF 186 EP D E+G+Y++FD ++W + +++ F Sbjct: 60 EPKTITDEF-EQGTYIYFDYEKWGQRKKEGFTF 91 >4BY6-C nrPDB Length = 262 Score = 99.9 bits (247), Expect = 5e-22, Method: Composition-based stats. Identities = 29/82 (35%), Positives = 46/82 (56%), Gaps = 6/82 (7%) Query: 107 FQDETLFFLFYKHPGTVIQELTYLEL-RKRNWRYHKTLKAWLTKDPMMEPIVSADGLSER 165 F +TLFF+FY + G+ Q L EL + RNW ++K + W K+ +E + G SE Sbjct: 174 FDLDTLFFIFYHYQGSYEQFLAARELFKNRNWLFNKVDRCWYYKE--IEKLPPGMGKSEE 231 Query: 166 GSYVFFDPQR-W--EKCQRDFL 184 S+ +FD ++ W +C DF+ Sbjct: 232 ESWRYFDYKKSWLARRCGNDFV 253 >1T7N-A nrPDB Length = 618 Score = 29.4 bits (64), Expect = 1.00, Method: Composition-based stats. Identities = 16/116 (13%), Positives = 39/116 (33%), Gaps = 23/116 (19%) Query: 10 VPLLKLEDKELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPWAETSRSEVE 69 + + ED Y+H+ G + +++ DH + T + + + Sbjct: 319 LQFIVAEDGSCGMVYEHAAAEGPPIVALV----------DHVMEYTKKPELVRSPMVPLP 368 Query: 70 PRFFTPESFTNIPGVLQSTVTPPCFNSIQNDQQRVALF--QDETLFFLFYKHPGTV 123 +P L+ +TP N I+ +Q +++ + + F+ Sbjct: 369 -----------MPKKLRFNITPEIKNDIEKAKQNLSIMIQDLDIMMLTFHHFGKDF 413 >6B18-A nrPDB Length = 308 Score = 29.0 bits (63), Expect = 1.2, Method: Composition-based stats. Identities = 18/108 (16%), Positives = 38/108 (35%), Gaps = 6/108 (5%) Query: 68 VEPRFFTPESFTNIPGVLQ--STVTPPCFNSIQNDQQRVALFQDETL-FFLFYKHPGTVI 124 V P + E + + + Q F I N +Q ++ L FFL Sbjct: 147 VHPEYLLSEQTSGVTAIEQVNQKFWDKRFQQINNFEQHISENGTIVLKFFLHVSKKEQKK 206 Query: 125 QELTYLELRKRNWRYHK---TLKAWLTKDPMMEPIVSADGLSERGSYV 169 + + +EL +NW++ +A + A+ +++ + Sbjct: 207 RFIERIELDTKNWKFSTGDLKERAHWKDYRNAYEDMLANTSTKQAPWF 254 >2WYA-A nrPDB Length = 460 Score = 28.2 bits (61), Expect = 2.3, Method: Composition-based stats. Identities = 14/64 (21%), Positives = 31/64 (48%) Query: 18 KELSSTYDHSMTLGADLSSMLYSLGIPRDSQDHRVLDTFQSPWAETSRSEVEPRFFTPES 77 +EL+ + + + G+ L++ +S + +D+ LD S ++ + + +PE Sbjct: 352 QELAGSRIGAFSYGSGLAASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLASRKCVSPEE 411 Query: 78 FTNI 81 FT I Sbjct: 412 FTEI 415 >3DKQ-A nrPDB Length = 243 Score = 27.5 bits (59), Expect = 3.5, Method: Composition-based stats. Identities = 24/118 (20%), Positives = 39/118 (33%), Gaps = 10/118 (8%) Query: 32 ADLSSMLYSLGIPRDSQDHRVLDTFQSPWAETSRSEVEPRFFTPESFTNIPGVLQSTVTP 91 DLS+ L+ L P + Q ++ S + S + VL T Sbjct: 130 TDLSATLF-LSEPENYQGGELVIQDTYGQQSIKLSAGSLVLYPSSSLHQVTPVLSGERTA 188 Query: 92 P---CFNSIQNDQQRVALFQDETLFFLFYKHPGTVIQELTYLELRKRNWRYHKTLKAW 146 + ++++ QR LFQ + Q EL + YH L+ W Sbjct: 189 AFMWLQSMVRDEGQRRLLFQLDQ------SIQSLTAQTAAEQELFNLSGVYHNLLRRW 240 >3ZKC-A nrPDB Length = 111 Score = 26.7 bits (57), Expect = 6.5, Method: Composition-based stats. Identities = 8/24 (33%), Positives = 14/24 (58%) Query: 159 ADGLSERGSYVFFDPQRWEKCQRD 182 G+S++ F D Q+W K Q++ Sbjct: 87 TSGVSKKQFREFLDYQKWRKSQKE 110 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.307 0.122 0.339 Lambda K H 0.267 0.0376 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,924,379 Number of Sequences: 36641 Number of extensions: 239659 Number of successful extensions: 679 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 640 Number of HSP's gapped (non-prelim): 24 length of query: 191 length of database: 10,336,785 effective HSP length: 92 effective length of query: 99 effective length of database: 6,965,813 effective search space: 689615487 effective search space used: 689615487 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.2 bits) S2: 56 (26.3 bits)