BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P04180 (440 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5BV7-A nrPDB 855 0.0 4X97-A nrPDB 393 e-110 5AA6-A nrPDB 32 0.72 4JCM-A nrPDB 30 2.9 1UFO-A nrPDB 29 3.1 4CCW-A nrPDB 29 4.7 5W1D-A nrPDB 28 7.1 6BXU-A nrPDB 28 7.9 >5BV7-A nrPDB Length = 422 Score = 855 bits (2210), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/416 (99%), Positives = 415/416 (99%) Query: 25 FWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTI 84 FWL +VLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTI Sbjct: 1 FWLFDVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTI 60 Query: 85 WLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGY 144 WLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGY Sbjct: 61 WLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGY 120 Query: 145 LHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGH 204 LHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGH Sbjct: 121 LHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGH 180 Query: 205 SLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLK 264 SLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLK Sbjct: 181 SLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLK 240 Query: 265 EEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDL 324 EEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDL Sbjct: 241 EEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDL 300 Query: 325 LAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQ 384 LAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQ Sbjct: 301 LAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQ 360 Query: 385 GRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE 440 GRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE Sbjct: 361 GRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE 416 >4X97-A nrPDB Length = 380 Score = 393 bits (1009), Expect = e-110, Method: Compositional matrix adjust. Identities = 186/375 (49%), Positives = 256/375 (68%), Gaps = 4/375 (1%) Query: 48 PVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVY 107 PV+LVPG LGNQLEAKLDKP VV+++C +KTE +FTIWL+L + LP+ +DCWIDN R+VY Sbjct: 7 PVVLVPGDLGNQLEAKLDKPTVVHYLCSKKTESYFTIWLNLELLLPVIIDCWIDNIRLVY 66 Query: 108 NRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSK--LAGYLHTLVQNLVNNGYVRDETVRA 165 N++S GV +RVPGFGKT+S+E+LD SK + Y HT+V++LV GY R E VR Sbjct: 67 NKTSRATQFPDGVDVRVPGFGKTFSLEFLDPSKSSVGSYFHTMVESLVGWGYTRGEDVRG 126 Query: 166 APYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDR 225 APYDWR P + Y+ L ++EEM+ YG PV L+ HS+G ++ LYFL RQPQAWKD+ Sbjct: 127 APYDWRRAPNENGPYFLALREMIEEMYQLYGGPVVLVAHSMGNMYTLYFLQRQPQAWKDK 186 Query: 226 FIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPE 285 +I F+SLGAPWGG K + VLASGDN IP++ +K++E+QR ++ W+ P W Sbjct: 187 YIRAFVSLGAPWGGVAKTLRVLASGDNNRIPVIGPLKIREQQRSAVSTSWLLPYNYTWSP 246 Query: 286 DHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPT 345 + VF+ TP+ NYT RD+++FF D+ FE+GW M + L+ PGV+++CLYG G+PT Sbjct: 247 EKVFVQTPTINYTLRDYRKFFQDIGFEDGWLMRQDTEGLVEATMPPGVQLHCLYGTGVPT 306 Query: 346 PRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMV 405 P ++ Y+ FP DP + + DGD TV +S C WQ RQ V L L G +H+ M+ Sbjct: 307 PDSFYYE-SFPDRDP-KICFGDGDGTVNLKSALQCQAWQSRQEHQVLLQELPGSEHIEML 364 Query: 406 FSNLTLEHINAILLG 420 + TL ++ +LLG Sbjct: 365 ANATTLAYLKRVLLG 379 >5AA6-A nrPDB Length = 597 Score = 31.6 bits (70), Expect = 0.72, Method: Compositional matrix adjust. Identities = 23/84 (27%), Positives = 36/84 (42%), Gaps = 3/84 (3%) Query: 239 GSIKPMLVLASGDNQGIPIMSSIK--LKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFN 296 G + +LA+ D GI + K + E + +T W+ PED +F+ P F Sbjct: 221 GPMVSQFMLANFDIDGIVVEPKAKTLVPEMEYMTGVDTWLNIQNGGPPEDTLFVDEPLFI 280 Query: 297 YTGRDFQRF-FADLHFEEGWYMWL 319 GRD F D+ + E + L Sbjct: 281 RNGRDLAALSFNDVLYTEAFRTIL 304 >4JCM-A nrPDB Length = 702 Score = 29.6 bits (65), Expect = 2.9, Method: Compositional matrix adjust. Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 4/44 (9%) Query: 161 ETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGH 204 + V + YD++L G+QE + +V+ H YG+ V+L+G+ Sbjct: 585 DGVTSNGYDFQLLTGKQE----SVRFVVDNAHTNYGENVYLVGN 624 >1UFO-A nrPDB Length = 238 Score = 29.3 bits (64), Expect = 3.1, Method: Compositional matrix adjust. Identities = 23/70 (32%), Positives = 33/70 (47%), Gaps = 11/70 (15%) Query: 170 WRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCL--HLLYFLLRQPQAWKDRFI 227 +R+ G +EE R + EE +G P+FL G SLG HLL + ++ R + Sbjct: 82 YRVALGFKEEARR----VAEEAERRFGLPLFLAGGSLGAFVAHLLL-----AEGFRPRGV 132 Query: 228 DGFISLGAPW 237 FI G P Sbjct: 133 LAFIGSGFPM 142 >4CCW-A nrPDB Length = 299 Score = 28.9 bits (63), Expect = 4.7, Method: Compositional matrix adjust. Identities = 14/27 (51%), Positives = 17/27 (62%) Query: 201 LIGHSLGCLHLLYFLLRQPQAWKDRFI 227 +IG SLG LH + FLLR P+ K I Sbjct: 125 MIGLSLGGLHTMNFLLRMPERVKSAAI 151 >5W1D-A nrPDB Length = 429 Score = 28.1 bits (61), Expect = 7.1, Method: Compositional matrix adjust. Identities = 18/45 (40%), Positives = 30/45 (66%), Gaps = 5/45 (11%) Query: 106 VYNRSSGLVSNAPGVQIRVPGFGKTY--SVEYLDSSKLAGYLHTL 148 V N+++GLVS APGV++ V G+TY +V+ D++ A H++ Sbjct: 165 VINKTTGLVSIAPGVELIV---GQTYALTVQASDNAPPAERRHSI 206 >6BXU-A nrPDB Length = 323 Score = 28.1 bits (61), Expect = 7.9, Method: Compositional matrix adjust. Identities = 18/45 (40%), Positives = 30/45 (66%), Gaps = 5/45 (11%) Query: 106 VYNRSSGLVSNAPGVQIRVPGFGKTY--SVEYLDSSKLAGYLHTL 148 V N+++GLVS APGV++ V G+TY +V+ D++ A H++ Sbjct: 59 VINKTTGLVSIAPGVELIV---GQTYALTVQASDNAPPAERRHSI 100 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5BV7-A nrPDB 821 0.0 4X97-A nrPDB 665 0.0 Sequences not found previously or not previously below threshold: 2ODR-C nrPDB 32 0.50 4V5G-AZ nrPDB 32 0.54 5AA6-A nrPDB 32 0.84 1Y7H-A nrPDB 31 1.3 6GAS-A nrPDB 31 1.4 3ILS-A nrPDB 30 2.1 4CCW-A nrPDB 29 3.9 CONVERGED! >5BV7-A nrPDB Length = 422 Score = 821 bits (2122), Expect = 0.0, Method: Composition-based stats. Identities = 414/416 (99%), Positives = 415/416 (99%) Query: 25 FWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTI 84 FWL +VLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTI Sbjct: 1 FWLFDVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTI 60 Query: 85 WLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGY 144 WLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGY Sbjct: 61 WLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGY 120 Query: 145 LHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGH 204 LHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGH Sbjct: 121 LHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGH 180 Query: 205 SLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLK 264 SLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLK Sbjct: 181 SLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLK 240 Query: 265 EEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDL 324 EEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDL Sbjct: 241 EEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDL 300 Query: 325 LAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQ 384 LAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQ Sbjct: 301 LAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQ 360 Query: 385 GRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE 440 GRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE Sbjct: 361 GRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE 416 >4X97-A nrPDB Length = 380 Score = 665 bits (1715), Expect = 0.0, Method: Composition-based stats. Identities = 186/375 (49%), Positives = 256/375 (68%), Gaps = 4/375 (1%) Query: 48 PVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVY 107 PV+LVPG LGNQLEAKLDKP VV+++C +KTE +FTIWL+L + LP+ +DCWIDN R+VY Sbjct: 7 PVVLVPGDLGNQLEAKLDKPTVVHYLCSKKTESYFTIWLNLELLLPVIIDCWIDNIRLVY 66 Query: 108 NRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSK--LAGYLHTLVQNLVNNGYVRDETVRA 165 N++S GV +RVPGFGKT+S+E+LD SK + Y HT+V++LV GY R E VR Sbjct: 67 NKTSRATQFPDGVDVRVPGFGKTFSLEFLDPSKSSVGSYFHTMVESLVGWGYTRGEDVRG 126 Query: 166 APYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDR 225 APYDWR P + Y+ L ++EEM+ YG PV L+ HS+G ++ LYFL RQPQAWKD+ Sbjct: 127 APYDWRRAPNENGPYFLALREMIEEMYQLYGGPVVLVAHSMGNMYTLYFLQRQPQAWKDK 186 Query: 226 FIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPE 285 +I F+SLGAPWGG K + VLASGDN IP++ +K++E+QR ++ W+ P W Sbjct: 187 YIRAFVSLGAPWGGVAKTLRVLASGDNNRIPVIGPLKIREQQRSAVSTSWLLPYNYTWSP 246 Query: 286 DHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPT 345 + VF+ TP+ NYT RD+++FF D+ FE+GW M + L+ PGV+++CLYG G+PT Sbjct: 247 EKVFVQTPTINYTLRDYRKFFQDIGFEDGWLMRQDTEGLVEATMPPGVQLHCLYGTGVPT 306 Query: 346 PRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMV 405 P ++ Y+ FP DP + + DGD TV +S C WQ RQ V L L G +H+ M+ Sbjct: 307 PDSFYYE-SFPDRDP-KICFGDGDGTVNLKSALQCQAWQSRQEHQVLLQELPGSEHIEML 364 Query: 406 FSNLTLEHINAILLG 420 + TL ++ +LLG Sbjct: 365 ANATTLAYLKRVLLG 379 >2ODR-C nrPDB Length = 701 Score = 31.9 bits (71), Expect = 0.50, Method: Composition-based stats. Identities = 20/81 (24%), Positives = 33/81 (40%), Gaps = 7/81 (8%) Query: 288 VFISTPSFNYTGRDFQRFFADLHFEEGWYMW---LQSRDLLAGLPAPGVEV--YCLYGVG 342 VF T N T + ++ + + FEE Y+ + RD+ + V C Y G Sbjct: 60 VFGKTHPVNDTIENLRQAYLRMGFEE--YINPVIVDERDIYKQFGPEAMAVLDRCFYLAG 117 Query: 343 LPTPRTYIYDHGFPYTDPVGV 363 LP P + D + +G+ Sbjct: 118 LPRPDVGLSDEKISQIEKLGI 138 >4V5G-AZ nrPDB Length = 406 Score = 31.9 bits (71), Expect = 0.54, Method: Composition-based stats. Identities = 11/31 (35%), Positives = 19/31 (61%) Query: 111 SGLVSNAPGVQIRVPGFGKTYSVEYLDSSKL 141 +G+V PGV++ +PG T++VE + L Sbjct: 351 TGVVQLPPGVEMVMPGDNVTFTVELIKPVAL 381 >5AA6-A nrPDB Length = 597 Score = 31.5 bits (70), Expect = 0.84, Method: Composition-based stats. Identities = 25/86 (29%), Positives = 38/86 (44%), Gaps = 7/86 (8%) Query: 237 WGGSIK-PML---VLASGDNQGIPIMSSIK--LKEEQRITTTSPWMFPSRMAWPEDHVFI 290 W G PM+ +LA+ D GI + K + E + +T W+ PED +F+ Sbjct: 215 WPGVSSGPMVSQFMLANFDIDGIVVEPKAKTLVPEMEYMTGVDTWLNIQNGGPPEDTLFV 274 Query: 291 STPSFNYTGRDFQRF-FADLHFEEGW 315 P F GRD F D+ + E + Sbjct: 275 DEPLFIRNGRDLAALSFNDVLYTEAF 300 >1Y7H-A nrPDB Length = 268 Score = 30.7 bits (68), Expect = 1.3, Method: Composition-based stats. Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 3/54 (5%) Query: 179 EYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQA-WKDRFIDGFI 231 +Y L L+E + A + V L+GHSLG ++L + + PQ + F+ F+ Sbjct: 57 DYTLPLMELMESLSA--DEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFM 108 >6GAS-A nrPDB Length = 331 Score = 30.7 bits (68), Expect = 1.4, Method: Composition-based stats. Identities = 23/90 (25%), Positives = 33/90 (36%), Gaps = 24/90 (26%) Query: 317 MWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYD-HGFPYT----------------D 359 M S ++ LP G ++ LY P YIYD GFP D Sbjct: 30 MRQASVKIIESLPQLGGQLSALY------PEKYIYDVAGFPKVRAQELVDNLKEQMKKFD 83 Query: 360 PVGVLYEDGDDTVATRSTELCGLWQGRQPQ 389 P V E+ DT+ ++ + L +Q Sbjct: 84 PT-VCLEEAVDTLEKQADGIFKLVTNKQTH 112 >3ILS-A nrPDB Length = 265 Score = 30.0 bits (66), Expect = 2.1, Method: Composition-based stats. Identities = 16/54 (29%), Positives = 24/54 (44%), Gaps = 3/54 (5%) Query: 384 QGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPP 437 QG + + ++ Q + + EH N+I G + P ASP S EPP Sbjct: 109 QGEEVHSLIIIDAPIPQAMEQLPRAF-YEHCNSI--GLFATQPGASPDGSTEPP 159 >4CCW-A nrPDB Length = 299 Score = 29.2 bits (64), Expect = 3.9, Method: Composition-based stats. Identities = 14/27 (51%), Positives = 17/27 (62%) Query: 201 LIGHSLGCLHLLYFLLRQPQAWKDRFI 227 +IG SLG LH + FLLR P+ K I Sbjct: 125 MIGLSLGGLHTMNFLLRMPERVKSAAI 151 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.308 0.139 0.449 Lambda K H 0.267 0.0425 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 21,014,471 Number of Sequences: 36641 Number of extensions: 941330 Number of successful extensions: 2441 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 2402 Number of HSP's gapped (non-prelim): 43 length of query: 440 length of database: 10,336,785 effective HSP length: 100 effective length of query: 340 effective length of database: 6,672,685 effective search space: 2268712900 effective search space used: 2268712900 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.1 bits) S2: 61 (28.1 bits)