BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P03882 (235 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3OOL-A nrPDB 483 e-137 2IFY-A nrPDB 32 0.25 1O99-A nrPDB 30 0.89 4V3P-LE nrPDB 28 2.7 5HX0-A nrPDB 28 3.1 3I1K-A nrPDB 27 8.2 >3OOL-A nrPDB Length = 237 Score = 483 bits (1243), Expect = e-137, Method: Compositional matrix adjust. Identities = 235/235 (100%), Positives = 235/235 (100%) Query: 1 MKNIKKNQVMNLGPNSKLLKEYKSQLIELNIEQFEAGIGLILGDAYIRSRDEGKTYCMQF 60 MKNIKKNQVMNLGPNSKLLKEYKSQLIELNIEQFEAGIGLILGDAYIRSRDEGKTYCMQF Sbjct: 3 MKNIKKNQVMNLGPNSKLLKEYKSQLIELNIEQFEAGIGLILGDAYIRSRDEGKTYCMQF 62 Query: 61 EWKNKAYMDHVCLLYDQWVLSPPHKKERVNHLGNLVITWGAQTFKHQAFNKLANLFIVNN 120 EWKNKAYMDHVCLLYDQWVLSPPHKKERVNHLGNLVITWGAQTFKHQAFNKLANLFIVNN Sbjct: 63 EWKNKAYMDHVCLLYDQWVLSPPHKKERVNHLGNLVITWGAQTFKHQAFNKLANLFIVNN 122 Query: 121 KKTIPNNLVENYLTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSFTFEEVEYLVKGLR 180 KKTIPNNLVENYLTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSFTFEEVEYLVKGLR Sbjct: 123 KKTIPNNLVENYLTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSFTFEEVEYLVKGLR 182 Query: 181 NKFQLNCYVKINKNKPIIYIDSMSYLIFYNLIKPYLIPQMMYKLPNTISSETFLK 235 NKFQLNCYVKINKNKPIIYIDSMSYLIFYNLIKPYLIPQMMYKLPNTISSETFLK Sbjct: 183 NKFQLNCYVKINKNKPIIYIDSMSYLIFYNLIKPYLIPQMMYKLPNTISSETFLK 237 >2IFY-A nrPDB Length = 508 Score = 32.0 bits (71), Expect = 0.25, Method: Compositional matrix adjust. Identities = 26/106 (24%), Positives = 46/106 (43%), Gaps = 8/106 (7%) Query: 111 KLANLFIVNNKKTIPNNLVENYLTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSFTFE 170 K A +I + I V + T +S Y+ MD +WD + ++N + T++ Sbjct: 156 KTAQSYIDATNEVIKETGVGQFAT-ISGRYYSMDRDKRWDRVEKCYRA--MVNGEGPTYK 212 Query: 171 EVEYLVK-----GLRNKFQLNCYVKINKNKPIIYIDSMSYLIFYNL 211 E V+ G+ ++F L + N P+ I+ +IFYN Sbjct: 213 SAEECVEDSYANGIYDEFVLPSVIVNEDNTPVATINDDDAVIFYNF 258 >1O99-A nrPDB Length = 511 Score = 30.0 bits (66), Expect = 0.89, Method: Compositional matrix adjust. Identities = 23/104 (22%), Positives = 44/104 (42%), Gaps = 8/104 (7%) Query: 133 LTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSF--TFEEVEYLVK-GLRNKFQLNCYV 189 + +S Y+ MD +WD + + + ++ E +E K G+ ++F L + Sbjct: 179 IATLSGRYYSMDRDKRWDRVEKAYRAMVYGEGPTYRDPLECIEDSYKHGIYDEFVLPSVI 238 Query: 190 KINKNKPIIYIDSMSYLIFYNLIKPYLIPQMMYKLPNTISSETF 233 +P+ I +IFYN P ++ NT ++E F Sbjct: 239 VREDGRPVATIQDNDAIIFYN-----FRPDRAIQISNTFTNEDF 277 >4V3P-LE nrPDB Length = 170 Score = 28.5 bits (62), Expect = 2.7, Method: Compositional matrix adjust. Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%) Query: 162 LNTQSFTFEEVEYLVK--GLRNKFQLNCYVKINKNKPIIYIDSMSYLIFYNLIK 213 L+ QS F + Y V+ G+R ++ CYV I K + ++S + Y L++ Sbjct: 42 LSGQSPVFSKARYTVRSFGIRRNEKIACYVTIRGEKAMQLLESGLKVKEYELLR 95 >5HX0-A nrPDB Length = 402 Score = 28.1 bits (61), Expect = 3.1, Method: Compositional matrix adjust. Identities = 25/108 (23%), Positives = 44/108 (40%), Gaps = 13/108 (12%) Query: 34 FEAGIGLILGDAYIRSRDEGKTYCMQFEWKNKAYMDHVCLLYDQWVLS---PPHKKERVN 90 F AG + LG +RS D GK+ W+ Y H+ + + P + + + Sbjct: 160 FLAGGAVFLGGYVLRSTDGGKS------WQGPLYPPHIEPEINYTAMGEKLPAYNRGAMY 213 Query: 91 HLGNLVITWGAQTFKHQAFNKLANLFIVNNKKTIPNNLVENYLTPMSL 138 N I W Q+ NK +N ++++ K L Y P+++ Sbjct: 214 EGKNGRILWVVAATDRQSPNKTSNHLLISDDK----GLTWKYSAPVAV 257 >3I1K-A nrPDB Length = 377 Score = 26.9 bits (58), Expect = 8.2, Method: Compositional matrix adjust. Identities = 19/70 (27%), Positives = 32/70 (45%), Gaps = 6/70 (8%) Query: 151 YNKNSTNKSIVLNTQSFTFEEVEYLV--KGLRNKFQLNCYVKINKNKPIIYIDSMSYLIF 208 YN + K ++ T+S+ + + Y V + +++ + N + I Y IF Sbjct: 241 YNATTFGKFLIYPTKSYCMDTMNYTVPVQAVQSIWSEN----RQSDDAIGQACKSPYCIF 296 Query: 209 YNLIKPYLIP 218 YN KPYL P Sbjct: 297 YNKTKPYLAP 306 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3OOL-A nrPDB 479 e-136 Sequences not found previously or not previously below threshold: 2IFY-A nrPDB 30 1.0 1O99-A nrPDB 28 3.3 6BMA-A nrPDB 28 4.3 3I1K-A nrPDB 28 4.5 4V3P-LE nrPDB 27 7.0 4FXG-H nrPDB 27 7.1 1ZJK-A nrPDB 27 7.1 1Q3X-A nrPDB 27 7.1 5HX0-A nrPDB 27 9.1 CONVERGED! >3OOL-A nrPDB Length = 237 Score = 479 bits (1234), Expect = e-136, Method: Composition-based stats. Identities = 235/235 (100%), Positives = 235/235 (100%) Query: 1 MKNIKKNQVMNLGPNSKLLKEYKSQLIELNIEQFEAGIGLILGDAYIRSRDEGKTYCMQF 60 MKNIKKNQVMNLGPNSKLLKEYKSQLIELNIEQFEAGIGLILGDAYIRSRDEGKTYCMQF Sbjct: 3 MKNIKKNQVMNLGPNSKLLKEYKSQLIELNIEQFEAGIGLILGDAYIRSRDEGKTYCMQF 62 Query: 61 EWKNKAYMDHVCLLYDQWVLSPPHKKERVNHLGNLVITWGAQTFKHQAFNKLANLFIVNN 120 EWKNKAYMDHVCLLYDQWVLSPPHKKERVNHLGNLVITWGAQTFKHQAFNKLANLFIVNN Sbjct: 63 EWKNKAYMDHVCLLYDQWVLSPPHKKERVNHLGNLVITWGAQTFKHQAFNKLANLFIVNN 122 Query: 121 KKTIPNNLVENYLTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSFTFEEVEYLVKGLR 180 KKTIPNNLVENYLTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSFTFEEVEYLVKGLR Sbjct: 123 KKTIPNNLVENYLTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSFTFEEVEYLVKGLR 182 Query: 181 NKFQLNCYVKINKNKPIIYIDSMSYLIFYNLIKPYLIPQMMYKLPNTISSETFLK 235 NKFQLNCYVKINKNKPIIYIDSMSYLIFYNLIKPYLIPQMMYKLPNTISSETFLK Sbjct: 183 NKFQLNCYVKINKNKPIIYIDSMSYLIFYNLIKPYLIPQMMYKLPNTISSETFLK 237 >2IFY-A nrPDB Length = 508 Score = 29.6 bits (65), Expect = 1.0, Method: Composition-based stats. Identities = 26/106 (24%), Positives = 46/106 (43%), Gaps = 8/106 (7%) Query: 111 KLANLFIVNNKKTIPNNLVENYLTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSFTFE 170 K A +I + I V + T +S Y+ MD +WD + ++N + T++ Sbjct: 156 KTAQSYIDATNEVIKETGVGQFAT-ISGRYYSMDRDKRWDRVEKCYRA--MVNGEGPTYK 212 Query: 171 EVEYLVK-----GLRNKFQLNCYVKINKNKPIIYIDSMSYLIFYNL 211 E V+ G+ ++F L + N P+ I+ +IFYN Sbjct: 213 SAEECVEDSYANGIYDEFVLPSVIVNEDNTPVATINDDDAVIFYNF 258 >1O99-A nrPDB Length = 511 Score = 28.1 bits (61), Expect = 3.3, Method: Composition-based stats. Identities = 23/104 (22%), Positives = 44/104 (42%), Gaps = 8/104 (7%) Query: 133 LTPMSLAYWFMDDGGKWDYNKNSTNKSIVLNTQSF--TFEEVEYLVK-GLRNKFQLNCYV 189 + +S Y+ MD +WD + + + ++ E +E K G+ ++F L + Sbjct: 179 IATLSGRYYSMDRDKRWDRVEKAYRAMVYGEGPTYRDPLECIEDSYKHGIYDEFVLPSVI 238 Query: 190 KINKNKPIIYIDSMSYLIFYNLIKPYLIPQMMYKLPNTISSETF 233 +P+ I +IFYN P ++ NT ++E F Sbjct: 239 VREDGRPVATIQDNDAIIFYN-----FRPDRAIQISNTFTNEDF 277 >6BMA-A nrPDB Length = 261 Score = 27.7 bits (60), Expect = 4.3, Method: Composition-based stats. Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 14/49 (28%) Query: 14 PNSKLL--------KEYKSQLIELNIEQFEAGIGLILGDAYIRSRDEGK 54 PNSK++ KE+ L L ++ F ++G+ ++R +DEGK Sbjct: 213 PNSKIIIAESGLENKEFLEHLQNLGVDAF------LIGEYFMREKDEGK 255 >3I1K-A nrPDB Length = 377 Score = 27.7 bits (60), Expect = 4.5, Method: Composition-based stats. Identities = 19/70 (27%), Positives = 32/70 (45%), Gaps = 6/70 (8%) Query: 151 YNKNSTNKSIVLNTQSFTFEEVEYLV--KGLRNKFQLNCYVKINKNKPIIYIDSMSYLIF 208 YN + K ++ T+S+ + + Y V + +++ + N + I Y IF Sbjct: 241 YNATTFGKFLIYPTKSYCMDTMNYTVPVQAVQSIWSEN----RQSDDAIGQACKSPYCIF 296 Query: 209 YNLIKPYLIP 218 YN KPYL P Sbjct: 297 YNKTKPYLAP 306 >4V3P-LE nrPDB Length = 170 Score = 26.9 bits (58), Expect = 7.0, Method: Composition-based stats. Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%) Query: 162 LNTQSFTFEEVEYLVK--GLRNKFQLNCYVKINKNKPIIYIDSMSYLIFYNLIK 213 L+ QS F + Y V+ G+R ++ CYV I K + ++S + Y L++ Sbjct: 42 LSGQSPVFSKARYTVRSFGIRRNEKIACYVTIRGEKAMQLLESGLKVKEYELLR 95 >4FXG-H nrPDB Length = 242 Score = 26.9 bits (58), Expect = 7.1, Method: Composition-based stats. Identities = 24/87 (27%), Positives = 32/87 (36%), Gaps = 26/87 (29%) Query: 73 LLYDQWVLSPPHKKERVNH--------LGNL-------VITWGAQTFKHQAFNKLAN--- 114 LLYD WVL+ H H +G L W F H+ + A Sbjct: 28 LLYDNWVLTAAHAVYEQKHDASALDIRMGTLKRLSPHYTQAWSEAVFIHEGYTHDAGFDN 87 Query: 115 ---LFIVNNKKTIPNNLVENYLTPMSL 138 L +NNK I +N +TP+ L Sbjct: 88 DIALIKLNNKVVINSN-----ITPICL 109 >1ZJK-A nrPDB Length = 403 Score = 26.9 bits (58), Expect = 7.1, Method: Composition-based stats. Identities = 24/87 (27%), Positives = 32/87 (36%), Gaps = 26/87 (29%) Query: 73 LLYDQWVLSPPHKKERVNH--------LGNL-------VITWGAQTFKHQAFNKLAN--- 114 LLYD WVL+ H H +G L W F H+ + A Sbjct: 189 LLYDNWVLTAAHAVYEQKHDASALDIRMGTLKRLSPHYTQAWSEAVFIHEGYTHDAGFDN 248 Query: 115 ---LFIVNNKKTIPNNLVENYLTPMSL 138 L +NNK I +N +TP+ L Sbjct: 249 DIALIKLNNKVVINSN-----ITPICL 270 >1Q3X-A nrPDB Length = 328 Score = 26.9 bits (58), Expect = 7.1, Method: Composition-based stats. Identities = 24/87 (27%), Positives = 32/87 (36%), Gaps = 26/87 (29%) Query: 73 LLYDQWVLSPPHKKERVNH--------LGNL-------VITWGAQTFKHQAFNKLAN--- 114 LLYD WVL+ H H +G L W F H+ + A Sbjct: 114 LLYDNWVLTAAHAVYEQKHDASALDIRMGTLKRLSPHYTQAWSEAVFIHEGYTHDAGFDN 173 Query: 115 ---LFIVNNKKTIPNNLVENYLTPMSL 138 L +NNK I +N +TP+ L Sbjct: 174 DIALIKLNNKVVINSN-----ITPICL 195 >5HX0-A nrPDB Length = 402 Score = 26.5 bits (57), Expect = 9.1, Method: Composition-based stats. Identities = 25/108 (23%), Positives = 44/108 (40%), Gaps = 13/108 (12%) Query: 34 FEAGIGLILGDAYIRSRDEGKTYCMQFEWKNKAYMDHVCLLYDQWVLS---PPHKKERVN 90 F AG + LG +RS D GK+ W+ Y H+ + + P + + + Sbjct: 160 FLAGGAVFLGGYVLRSTDGGKS------WQGPLYPPHIEPEINYTAMGEKLPAYNRGAMY 213 Query: 91 HLGNLVITWGAQTFKHQAFNKLANLFIVNNKKTIPNNLVENYLTPMSL 138 N I W Q+ NK +N ++++ K L Y P+++ Sbjct: 214 EGKNGRILWVVAATDRQSPNKTSNHLLISDDK----GLTWKYSAPVAV 257 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.136 0.415 Lambda K H 0.267 0.0439 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,824,182 Number of Sequences: 36641 Number of extensions: 497592 Number of successful extensions: 985 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 982 Number of HSP's gapped (non-prelim): 20 length of query: 235 length of database: 10,336,785 effective HSP length: 94 effective length of query: 141 effective length of database: 6,892,531 effective search space: 971846871 effective search space used: 971846871 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 58 (26.9 bits)