BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P02920 (417 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5GXB-A nrPDB 824 0.0 5CM5-A nrPDB 32 0.43 3WDO-A nrPDB 32 0.52 >5GXB-A nrPDB Length = 423 Score = 824 bits (2128), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/417 (99%), Positives = 415/417 (99%) Query: 1 MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQ 60 MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT IIFAAISLFSLLFQ Sbjct: 1 MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTWIIFAAISLFSLLFQ 60 Query: 61 PLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNA 120 PLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNA Sbjct: 61 PLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNA 120 Query: 121 GAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALIL 180 GAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALIL Sbjct: 121 GAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALIL 180 Query: 181 AVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD 240 AVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD Sbjct: 181 AVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD 240 Query: 241 QQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMS 300 QQFANFFTSFFATGEQGTRVF YVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMS Sbjct: 241 QQFANFFTSFFATGEQGTRVFWYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMS 300 Query: 301 VRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQL 360 VRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQL Sbjct: 301 VRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQL 360 Query: 361 AMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA 417 AMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA Sbjct: 361 AMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA 417 >5CM5-A nrPDB Length = 277 Score = 32.3 bits (72), Expect = 0.43, Method: Compositional matrix adjust. Identities = 17/37 (45%), Positives = 20/37 (54%) Query: 97 PLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVS 133 PLL G ++GGI GF F P +EA IE VS Sbjct: 180 PLLARVTGAGCLLGGIIAGFLFRETEPDIEALIEAVS 216 >3WDO-A nrPDB Length = 453 Score = 32.0 bits (71), Expect = 0.52, Method: Compositional matrix adjust. Identities = 36/128 (28%), Positives = 52/128 (40%), Gaps = 10/128 (7%) Query: 10 WMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDK 69 W G F M P + + S++ GI L +FQ FGLLSD+ Sbjct: 13 WGLGTVFSLRMLGMFMVLPVLTTYGMALQGASEALIGIAIGIYGLTQAVFQIPFGLLSDR 72 Query: 70 LGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFI 129 +G RK L I G L +FA + + I++G + G A A AV A + Sbjct: 73 IG-RKPL---IVGGLAVFAAGSVIAALSDSIWGIILGRALQG------SGAIAAAVMALL 122 Query: 130 EKVSRRSN 137 ++R N Sbjct: 123 SDLTREQN 130 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5GXB-A nrPDB 669 0.0 Sequences not found previously or not previously below threshold: 5CM5-A nrPDB 32 0.39 3WDO-A nrPDB 28 8.2 CONVERGED! >5GXB-A nrPDB Length = 423 Score = 669 bits (1726), Expect = 0.0, Method: Composition-based stats. Identities = 415/417 (99%), Positives = 415/417 (99%) Query: 1 MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQ 60 MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT IIFAAISLFSLLFQ Sbjct: 1 MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTWIIFAAISLFSLLFQ 60 Query: 61 PLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNA 120 PLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNA Sbjct: 61 PLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNA 120 Query: 121 GAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALIL 180 GAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALIL Sbjct: 121 GAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALIL 180 Query: 181 AVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD 240 AVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD Sbjct: 181 AVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD 240 Query: 241 QQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMS 300 QQFANFFTSFFATGEQGTRVF YVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMS Sbjct: 241 QQFANFFTSFFATGEQGTRVFWYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMS 300 Query: 301 VRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQL 360 VRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQL Sbjct: 301 VRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQL 360 Query: 361 AMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA 417 AMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA Sbjct: 361 AMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA 417 >5CM5-A nrPDB Length = 277 Score = 32.2 bits (72), Expect = 0.39, Method: Composition-based stats. Identities = 17/37 (45%), Positives = 20/37 (54%) Query: 97 PLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVS 133 PLL G ++GGI GF F P +EA IE VS Sbjct: 180 PLLARVTGAGCLLGGIIAGFLFRETEPDIEALIEAVS 216 >3WDO-A nrPDB Length = 453 Score = 27.9 bits (61), Expect = 8.2, Method: Composition-based stats. Identities = 33/128 (25%), Positives = 51/128 (39%), Gaps = 10/128 (7%) Query: 10 WMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDK 69 W G F M P + + S++ GI L +FQ FGLLSD+ Sbjct: 13 WGLGTVFSLRMLGMFMVLPVLTTYGMALQGASEALIGIAIGIYGLTQAVFQIPFGLLSDR 72 Query: 70 LGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFI 129 +G + +I G L +FA + + I++G + G A A AV A + Sbjct: 73 IGRKP----LIVGGLAVFAAGSVIAALSDSIWGIILGRALQGS------GAIAAAVMALL 122 Query: 130 EKVSRRSN 137 ++R N Sbjct: 123 SDLTREQN 130 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.327 0.153 0.507 Lambda K H 0.267 0.0479 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 20,309,369 Number of Sequences: 36641 Number of extensions: 1033758 Number of successful extensions: 3034 Number of sequences better than 10.0: 27 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 29 Number of HSP's that attempted gapping in prelim test: 2997 Number of HSP's gapped (non-prelim): 69 length of query: 417 length of database: 10,336,785 effective HSP length: 100 effective length of query: 317 effective length of database: 6,672,685 effective search space: 2115241145 effective search space used: 2115241145 T: 11 A: 40 X1: 16 ( 7.6 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.1 bits) S2: 61 (27.9 bits)