BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P02656 (99 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2JQ3-A nrPDB 162 4e-41 4V6W-AK nrPDB 28 1.0 4O2Z-A nrPDB 27 1.4 1TUZ-A nrPDB 26 5.0 1I7H-A nrPDB 25 5.0 3SQ8-A nrPDB 25 7.3 1Q32-A nrPDB 25 8.0 5E8V-A nrPDB 25 8.7 4AXK-A nrPDB 25 9.9 >2JQ3-A nrPDB Length = 79 Score = 162 bits (409), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 79/79 (100%), Positives = 79/79 (100%) Query: 21 SEAEDASLLSFMQGYMKHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVKDK 80 SEAEDASLLSFMQGYMKHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVKDK Sbjct: 1 SEAEDASLLSFMQGYMKHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVKDK 60 Query: 81 FSEFWDLDPEVRPTSAVAA 99 FSEFWDLDPEVRPTSAVAA Sbjct: 61 FSEFWDLDPEVRPTSAVAA 79 >4V6W-AK nrPDB Length = 163 Score = 27.7 bits (60), Expect = 1.0, Method: Compositional matrix adjust. Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 5/56 (8%) Query: 45 DALSSVQESQVAQ--QARGWVTDGFSSLKDYWSTVKDKFSE---FWDLDPEVRPTS 95 D + ++Q +V Q +RGWV + F+ YW + E + L PE+ P++ Sbjct: 36 DKIPNLQVIKVMQSLNSRGWVKEQFAWRHFYWLLTNEGIEELRRYLHLPPEIVPST 91 >4O2Z-A nrPDB Length = 377 Score = 27.3 bits (59), Expect = 1.4, Method: Composition-based stats. Identities = 10/18 (55%), Positives = 12/18 (66%) Query: 79 DKFSEFWDLDPEVRPTSA 96 D S+ W LDP RPT+A Sbjct: 309 DFLSKMWTLDPSKRPTAA 326 >1TUZ-A nrPDB Length = 118 Score = 25.8 bits (55), Expect = 5.0, Method: Compositional matrix adjust. Identities = 9/30 (30%), Positives = 19/30 (63%) Query: 32 MQGYMKHATKTAKDALSSVQESQVAQQARG 61 +Q YM+++TK D L ++ ++A+ +G Sbjct: 16 LQKYMEYSTKKVSDVLKLFEDGEMAKYVQG 45 >1I7H-A nrPDB Length = 111 Score = 25.4 bits (54), Expect = 5.0, Method: Compositional matrix adjust. Identities = 12/44 (27%), Positives = 19/44 (43%) Query: 49 SVQESQVAQQARGWVTDGFSSLKDYWSTVKDKFSEFWDLDPEVR 92 + ++S V +GF SL + D + W L+PE R Sbjct: 41 ACEKSCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESR 84 >3SQ8-A nrPDB Length = 470 Score = 25.0 bits (53), Expect = 7.3, Method: Composition-based stats. Identities = 10/37 (27%), Positives = 18/37 (48%) Query: 53 SQVAQQARGWVTDGFSSLKDYWSTVKDKFSEFWDLDP 89 S + + GW + K Y+ +++KF F+ DP Sbjct: 306 SPLKWSSSGWFHFQYLQKKSYYEMLRNKFKVFYKQDP 342 >1Q32-A nrPDB Length = 544 Score = 25.0 bits (53), Expect = 8.0, Method: Composition-based stats. Identities = 10/37 (27%), Positives = 18/37 (48%) Query: 53 SQVAQQARGWVTDGFSSLKDYWSTVKDKFSEFWDLDP 89 S + + GW + K Y+ +++KF F+ DP Sbjct: 383 SPLKWSSSGWFHFQYLQKKSYYEMLRNKFKVFYKQDP 419 >5E8V-A nrPDB Length = 316 Score = 24.6 bits (52), Expect = 8.7, Method: Compositional matrix adjust. Identities = 10/23 (43%), Positives = 13/23 (56%) Query: 77 VKDKFSEFWDLDPEVRPTSAVAA 99 V + +E WD DPE R T+ A Sbjct: 280 VCETLTECWDHDPEARLTAQCVA 302 >4AXK-A nrPDB Length = 250 Score = 24.6 bits (52), Expect = 9.9, Method: Compositional matrix adjust. Identities = 11/42 (26%), Positives = 19/42 (45%), Gaps = 4/42 (9%) Query: 37 KHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVK 78 +H K A D ++ + + GWV+DG D W ++ Sbjct: 119 RHGEKIAVDIAVRLENGEWRTKGNGWVSDG----GDLWEVLE 156 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2JQ3-A nrPDB 146 2e-36 Sequences not found previously or not previously below threshold: 4V6W-AK nrPDB 30 0.29 3ZQS-A nrPDB 29 0.68 4O2Z-A nrPDB 28 1.2 2Y7J-A nrPDB 26 2.8 5E8V-A nrPDB 26 2.9 1TUZ-A nrPDB 26 3.0 3SQ8-A nrPDB 26 4.0 1Q32-A nrPDB 26 4.0 2XGV-A nrPDB 26 4.9 1I7H-A nrPDB 26 5.1 4AXK-A nrPDB 26 5.4 CONVERGED! >2JQ3-A nrPDB Length = 79 Score = 146 bits (369), Expect = 2e-36, Method: Composition-based stats. Identities = 79/79 (100%), Positives = 79/79 (100%) Query: 21 SEAEDASLLSFMQGYMKHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVKDK 80 SEAEDASLLSFMQGYMKHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVKDK Sbjct: 1 SEAEDASLLSFMQGYMKHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVKDK 60 Query: 81 FSEFWDLDPEVRPTSAVAA 99 FSEFWDLDPEVRPTSAVAA Sbjct: 61 FSEFWDLDPEVRPTSAVAA 79 >4V6W-AK nrPDB Length = 163 Score = 29.8 bits (65), Expect = 0.29, Method: Composition-based stats. Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 5/56 (8%) Query: 45 DALSSVQESQVAQ--QARGWVTDGFSSLKDYWSTVKDKFSE---FWDLDPEVRPTS 95 D + ++Q +V Q +RGWV + F+ YW + E + L PE+ P++ Sbjct: 36 DKIPNLQVIKVMQSLNSRGWVKEQFAWRHFYWLLTNEGIEELRRYLHLPPEIVPST 91 >3ZQS-A nrPDB Length = 186 Score = 28.6 bits (62), Expect = 0.68, Method: Composition-based stats. Identities = 12/30 (40%), Positives = 17/30 (56%) Query: 69 SLKDYWSTVKDKFSEFWDLDPEVRPTSAVA 98 SLK +W + + + W L+PE P SA A Sbjct: 88 SLKAFWDVMDEIDEKTWVLEPEKPPRSATA 117 >4O2Z-A nrPDB Length = 377 Score = 27.8 bits (60), Expect = 1.2, Method: Composition-based stats. Identities = 10/18 (55%), Positives = 12/18 (66%) Query: 79 DKFSEFWDLDPEVRPTSA 96 D S+ W LDP RPT+A Sbjct: 309 DFLSKMWTLDPSKRPTAA 326 >2Y7J-A nrPDB Length = 365 Score = 26.3 bits (56), Expect = 2.8, Method: Composition-based stats. Identities = 13/27 (48%), Positives = 15/27 (55%) Query: 72 DYWSTVKDKFSEFWDLDPEVRPTSAVA 98 D STVKD S +DPE R T+ A Sbjct: 331 DRSSTVKDLISRLLQVDPEARLTAEQA 357 >5E8V-A nrPDB Length = 316 Score = 26.3 bits (56), Expect = 2.9, Method: Composition-based stats. Identities = 29/111 (26%), Positives = 44/111 (39%), Gaps = 12/111 (10%) Query: 1 MQPRVLLVVALLALLASARASEAEDASLLSFMQGYMKHATKTAKDAL----SSVQESQ-V 55 M P VL L + S + ++ +L+ + +A KD S V+E V Sbjct: 192 MAPEVLASAMNLENVESFKQTDVYSMALVLWEMTSRCNAVGEVKDYEPPFGSKVREHPCV 251 Query: 56 AQQARGWVTD-GFSSLKDYW------STVKDKFSEFWDLDPEVRPTSAVAA 99 A A + D G + +W V + +E WD DPE R T+ A Sbjct: 252 ASMADNVLADAGRPEIPSFWLNHQGIQMVCETLTECWDHDPEARLTAQCVA 302 >1TUZ-A nrPDB Length = 118 Score = 26.3 bits (56), Expect = 3.0, Method: Composition-based stats. Identities = 9/30 (30%), Positives = 19/30 (63%) Query: 32 MQGYMKHATKTAKDALSSVQESQVAQQARG 61 +Q YM+++TK D L ++ ++A+ +G Sbjct: 16 LQKYMEYSTKKVSDVLKLFEDGEMAKYVQG 45 >3SQ8-A nrPDB Length = 470 Score = 25.9 bits (55), Expect = 4.0, Method: Composition-based stats. Identities = 10/37 (27%), Positives = 18/37 (48%) Query: 53 SQVAQQARGWVTDGFSSLKDYWSTVKDKFSEFWDLDP 89 S + + GW + K Y+ +++KF F+ DP Sbjct: 306 SPLKWSSSGWFHFQYLQKKSYYEMLRNKFKVFYKQDP 342 >1Q32-A nrPDB Length = 544 Score = 25.9 bits (55), Expect = 4.0, Method: Composition-based stats. Identities = 10/37 (27%), Positives = 18/37 (48%) Query: 53 SQVAQQARGWVTDGFSSLKDYWSTVKDKFSEFWDLDP 89 S + + GW + K Y+ +++KF F+ DP Sbjct: 383 SPLKWSSSGWFHFQYLQKKSYYEMLRNKFKVFYKQDP 419 >2XGV-A nrPDB Length = 142 Score = 25.5 bits (54), Expect = 4.9, Method: Composition-based stats. Identities = 15/53 (28%), Positives = 26/53 (49%), Gaps = 1/53 (1%) Query: 18 ARASEAEDASLLSFMQGYMKHATKTAKDALSSVQESQVAQQARGWVTDGFSSL 70 AR +A+ L + +++ T A+S+ Q + AQ R W+T G +L Sbjct: 77 AREWDAQPQQPLPYTSAHIRGLTGDQAFAISA-QGREAAQVFRAWITQGLMNL 128 >1I7H-A nrPDB Length = 111 Score = 25.5 bits (54), Expect = 5.1, Method: Composition-based stats. Identities = 11/30 (36%), Positives = 15/30 (50%) Query: 63 VTDGFSSLKDYWSTVKDKFSEFWDLDPEVR 92 V +GF SL + D + W L+PE R Sbjct: 55 VREGFDSLPESSEQEDDMLDKAWGLEPESR 84 >4AXK-A nrPDB Length = 250 Score = 25.5 bits (54), Expect = 5.4, Method: Composition-based stats. Identities = 11/42 (26%), Positives = 19/42 (45%), Gaps = 4/42 (9%) Query: 37 KHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVK 78 +H K A D ++ + + GWV+DG D W ++ Sbjct: 119 RHGEKIAVDIAVRLENGEWRTKGNGWVSDG----GDLWEVLE 156 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.124 0.351 Lambda K H 0.267 0.0378 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,266,535 Number of Sequences: 36641 Number of extensions: 93751 Number of successful extensions: 328 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 312 Number of HSP's gapped (non-prelim): 24 length of query: 99 length of database: 10,336,785 effective HSP length: 67 effective length of query: 32 effective length of database: 7,881,838 effective search space: 252218816 effective search space used: 252218816 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.8 bits)