BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P01050 (65 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1HRT-I nrPDB 132 4e-32 4HTC-I nrPDB 120 1e-28 2JOO-A nrPDB 112 5e-26 2QK4-A nrPDB 26 4.9 >1HRT-I nrPDB Length = 65 Score = 132 bits (331), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 65/65 (100%), Positives = 65/65 (100%) Query: 1 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIP 60 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIP Sbjct: 1 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIP 60 Query: 61 EEYLQ 65 EEYLQ Sbjct: 61 EEYLQ 65 >4HTC-I nrPDB Length = 65 Score = 120 bits (301), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 57/65 (87%), Positives = 63/65 (96%) Query: 1 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIP 60 + YTDCTESGQNLCLCEGSNVCG+GNKCILGS+G+ NQCVTGEGTPKP+SHN+GDFEEIP Sbjct: 1 ITYTDCTESGQNLCLCEGSNVCGKGNKCILGSNGKGNQCVTGEGTPKPESHNNGDFEEIP 60 Query: 61 EEYLQ 65 EEYLQ Sbjct: 61 EEYLQ 65 >2JOO-A nrPDB Length = 66 Score = 112 bits (279), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 55/62 (88%), Positives = 56/62 (90%) Query: 1 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIP 60 VVYTDCTESGQNLCLCEGSNVCGQGNKCILG KNQCVTGEGTPKPQSHN GDFE IP Sbjct: 1 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGRGDSKNQCVTGEGTPKPQSHNQGDFEPIP 60 Query: 61 EE 62 E+ Sbjct: 61 ED 62 >2QK4-A nrPDB Length = 452 Score = 25.8 bits (55), Expect = 4.9, Method: Composition-based stats. Identities = 16/45 (35%), Positives = 19/45 (42%), Gaps = 4/45 (8%) Query: 13 LCLCEGSNVC----GQGNKCILGSDGEKNQCVTGEGTPKPQSHND 53 LC +G V Q +K +L DG N G P PQ ND Sbjct: 223 LCFTDGKTVAPMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSND 267 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1HRT-I nrPDB 108 7e-25 4HTC-I nrPDB 105 6e-24 2JOO-A nrPDB 104 1e-23 Sequences not found previously or not previously below threshold: 5T5M-B nrPDB 27 2.3 CONVERGED! >1HRT-I nrPDB Length = 65 Score = 108 bits (269), Expect = 7e-25, Method: Composition-based stats. Identities = 65/65 (100%), Positives = 65/65 (100%) Query: 1 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIP 60 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIP Sbjct: 1 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIP 60 Query: 61 EEYLQ 65 EEYLQ Sbjct: 61 EEYLQ 65 >4HTC-I nrPDB Length = 65 Score = 105 bits (261), Expect = 6e-24, Method: Composition-based stats. Identities = 57/65 (87%), Positives = 63/65 (96%) Query: 1 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIP 60 + YTDCTESGQNLCLCEGSNVCG+GNKCILGS+G+ NQCVTGEGTPKP+SHN+GDFEEIP Sbjct: 1 ITYTDCTESGQNLCLCEGSNVCGKGNKCILGSNGKGNQCVTGEGTPKPESHNNGDFEEIP 60 Query: 61 EEYLQ 65 EEYLQ Sbjct: 61 EEYLQ 65 >2JOO-A nrPDB Length = 66 Score = 104 bits (259), Expect = 1e-23, Method: Composition-based stats. Identities = 55/62 (88%), Positives = 56/62 (90%) Query: 1 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGTPKPQSHNDGDFEEIP 60 VVYTDCTESGQNLCLCEGSNVCGQGNKCILG KNQCVTGEGTPKPQSHN GDFE IP Sbjct: 1 VVYTDCTESGQNLCLCEGSNVCGQGNKCILGRGDSKNQCVTGEGTPKPQSHNQGDFEPIP 60 Query: 61 EE 62 E+ Sbjct: 61 ED 62 >5T5M-B nrPDB Length = 432 Score = 26.9 bits (58), Expect = 2.3, Method: Composition-based stats. Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 7/53 (13%) Query: 13 LCLCEGSNVCGQGNKCILGSDGEKNQCVTGEGT---PKPQSHNDGDFEEIPEE 62 +C EG+ + G N C +G ++ V EG KP +G+F E+ + Sbjct: 20 ICKVEGNEIVGTINACRIGH----SKFVHAEGAMRYKKPLIRKNGEFVEVSYD 68 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.307 0.140 0.444 Lambda K H 0.267 0.0412 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,198,792 Number of Sequences: 36641 Number of extensions: 117978 Number of successful extensions: 173 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 164 Number of HSP's gapped (non-prelim): 11 length of query: 65 length of database: 10,336,785 effective HSP length: 36 effective length of query: 29 effective length of database: 9,017,709 effective search space: 261513561 effective search space used: 261513561 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.5 bits) S2: 53 (25.0 bits)