BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P00424 (153 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6GIQ-e nrPDB 315 3e-87 6HU9-e nrPDB 274 9e-75 1K0G-A nrPDB 30 0.33 2C0F-A nrPDB 30 0.53 >6GIQ-e nrPDB Length = 153 Score = 315 bits (808), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 153/153 (100%), Positives = 153/153 (100%) Query: 1 MLRNTFTRAGGLSRITSVRFAQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLP 60 MLRNTFTRAGGLSRITSVRFAQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLP Sbjct: 1 MLRNTFTRAGGLSRITSVRFAQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLP 60 Query: 61 WAQLTEPEKQAVWYISYGEWGPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQD 120 WAQLTEPEKQAVWYISYGEWGPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQD Sbjct: 61 WAQLTEPEKQAVWYISYGEWGPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQD 120 Query: 121 AKTMNKEWQLKSDEYLKSKNANPWGGYSQVQSK 153 AKTMNKEWQLKSDEYLKSKNANPWGGYSQVQSK Sbjct: 121 AKTMNKEWQLKSDEYLKSKNANPWGGYSQVQSK 153 >6HU9-e nrPDB Length = 133 Score = 274 bits (701), Expect = 9e-75, Method: Compositional matrix adjust. Identities = 133/133 (100%), Positives = 133/133 (100%) Query: 21 AQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQAVWYISYGEW 80 AQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQAVWYISYGEW Sbjct: 1 AQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQAVWYISYGEW 60 Query: 81 GPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQDAKTMNKEWQLKSDEYLKSKN 140 GPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQDAKTMNKEWQLKSDEYLKSKN Sbjct: 61 GPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQDAKTMNKEWQLKSDEYLKSKN 120 Query: 141 ANPWGGYSQVQSK 153 ANPWGGYSQVQSK Sbjct: 121 ANPWGGYSQVQSK 133 >1K0G-A nrPDB Length = 453 Score = 30.4 bits (67), Expect = 0.33, Method: Compositional matrix adjust. Identities = 15/52 (28%), Positives = 26/52 (50%) Query: 33 DLQSRWENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQAVWYISYGEWGPRR 84 DL R+E++P +QDIV WA + + ++ V +S+ + RR Sbjct: 113 DLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDVNARR 164 >2C0F-A nrPDB Length = 248 Score = 29.6 bits (65), Expect = 0.53, Method: Compositional matrix adjust. Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 6/42 (14%) Query: 38 WENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQ--AVWYISY 77 + N+P EQ ++ KL +Q+ QLT+PE+Q A Y+ Y Sbjct: 151 YANIPDAEQLKLIEKLQAKQE----QLTDPEQQQNARAYLIY 188 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6GIQ-e nrPDB 316 2e-87 6HU9-e nrPDB 273 2e-74 Sequences not found previously or not previously below threshold: 2C0F-A nrPDB 31 0.19 1K0G-A nrPDB 30 0.27 5UBM-A nrPDB 27 2.6 1ELV-A nrPDB 27 2.6 3UFM-A nrPDB 26 5.2 4AM6-A nrPDB 26 6.8 CONVERGED! >6GIQ-e nrPDB Length = 153 Score = 316 bits (809), Expect = 2e-87, Method: Composition-based stats. Identities = 153/153 (100%), Positives = 153/153 (100%) Query: 1 MLRNTFTRAGGLSRITSVRFAQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLP 60 MLRNTFTRAGGLSRITSVRFAQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLP Sbjct: 1 MLRNTFTRAGGLSRITSVRFAQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLP 60 Query: 61 WAQLTEPEKQAVWYISYGEWGPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQD 120 WAQLTEPEKQAVWYISYGEWGPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQD Sbjct: 61 WAQLTEPEKQAVWYISYGEWGPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQD 120 Query: 121 AKTMNKEWQLKSDEYLKSKNANPWGGYSQVQSK 153 AKTMNKEWQLKSDEYLKSKNANPWGGYSQVQSK Sbjct: 121 AKTMNKEWQLKSDEYLKSKNANPWGGYSQVQSK 153 >6HU9-e nrPDB Length = 133 Score = 273 bits (698), Expect = 2e-74, Method: Composition-based stats. Identities = 133/133 (100%), Positives = 133/133 (100%) Query: 21 AQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQAVWYISYGEW 80 AQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQAVWYISYGEW Sbjct: 1 AQTHALSNAAVMDLQSRWENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQAVWYISYGEW 60 Query: 81 GPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQDAKTMNKEWQLKSDEYLKSKN 140 GPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQDAKTMNKEWQLKSDEYLKSKN Sbjct: 61 GPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAVVRMAGGQDAKTMNKEWQLKSDEYLKSKN 120 Query: 141 ANPWGGYSQVQSK 153 ANPWGGYSQVQSK Sbjct: 121 ANPWGGYSQVQSK 133 >2C0F-A nrPDB Length = 248 Score = 31.3 bits (69), Expect = 0.19, Method: Composition-based stats. Identities = 13/33 (39%), Positives = 21/33 (63%), Gaps = 4/33 (12%) Query: 38 WENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQ 70 + N+P EQ ++ KL +Q+ QLT+PE+Q Sbjct: 151 YANIPDAEQLKLIEKLQAKQE----QLTDPEQQ 179 >1K0G-A nrPDB Length = 453 Score = 30.5 bits (67), Expect = 0.27, Method: Composition-based stats. Identities = 16/55 (29%), Positives = 27/55 (49%) Query: 33 DLQSRWENMPSTEQQDIVSKLSERQKLPWAQLTEPEKQAVWYISYGEWGPRRPVL 87 DL R+E++P +QDIV WA + + ++ V +S+ + RR L Sbjct: 113 DLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDVNARRAWL 167 >5UBM-A nrPDB Length = 252 Score = 27.4 bits (59), Expect = 2.6, Method: Composition-based stats. Identities = 13/33 (39%), Positives = 15/33 (45%), Gaps = 8/33 (24%) Query: 114 RMAGGQDAKTMNKEWQLKSDEYLKSKNANPWGG 146 R+ GG DA N WQ+ D NPW G Sbjct: 1 RIIGGSDADIKNFPWQVFFD--------NPWAG 25 >1ELV-A nrPDB Length = 333 Score = 27.4 bits (59), Expect = 2.6, Method: Composition-based stats. Identities = 13/33 (39%), Positives = 15/33 (45%), Gaps = 8/33 (24%) Query: 114 RMAGGQDAKTMNKEWQLKSDEYLKSKNANPWGG 146 R+ GG DA N WQ+ D NPW G Sbjct: 82 RIIGGSDADIKNFPWQVFFD--------NPWAG 106 >3UFM-A nrPDB Length = 255 Score = 26.2 bits (56), Expect = 5.2, Method: Composition-based stats. Identities = 15/52 (28%), Positives = 24/52 (46%), Gaps = 7/52 (13%) Query: 109 LFAVVRMAGGQDAKTMNKEWQLKSDEYLKSKNANP-------WGGYSQVQSK 153 L AV+ + GQ K W+ +D +K+ NA WG Y++ + K Sbjct: 141 LNAVLTVRAGQANSHQGKGWEHFTDAVIKAVNAKEERVVFILWGSYARKKKK 192 >4AM6-A nrPDB Length = 655 Score = 25.9 bits (55), Expect = 6.8, Method: Composition-based stats. Identities = 24/101 (23%), Positives = 42/101 (41%), Gaps = 4/101 (3%) Query: 53 LSERQKLPWAQLTEPEKQAVWYISYGEWGPRRPVLNKGDSSFIAKGVAAGLLFSVGLFAV 112 L+ER K + + + AV ++ P +P F +A LF +F + Sbjct: 335 LAERLKKNFTTFQDAD-VAVQLYNFMNRSPNQPTEKYEFKLFDEVMLAPLALFFPQIFKL 393 Query: 113 VRMAGGQDAKTMNKEWQLKSDEYLKSKNANPWGGYSQVQSK 153 +R + +++ E+QL L + N W SQ +SK Sbjct: 394 IRTSSHKNSSL---EFQLPESRDLFTNELNDWNSLSQFESK 431 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.128 0.384 Lambda K H 0.267 0.0391 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,003,189 Number of Sequences: 36641 Number of extensions: 219832 Number of successful extensions: 628 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 624 Number of HSP's gapped (non-prelim): 16 length of query: 153 length of database: 10,336,785 effective HSP length: 89 effective length of query: 64 effective length of database: 7,075,736 effective search space: 452847104 effective search space used: 452847104 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 54 (25.5 bits)