BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= P00128 (127 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6GIQ-G nrPDB 261 8e-71 1BCC-F nrPDB 74 2e-14 3CWB-F nrPDB 70 2e-13 4D6U-F nrPDB 69 6e-13 5XTI-AF nrPDB 68 9e-13 6HAW-F nrPDB 67 2e-12 >6GIQ-G nrPDB Length = 127 Score = 261 bits (666), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 127/127 (100%), Positives = 127/127 (100%) Query: 1 MPQSFTSIARIGDYILKSPVLSKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTAL 60 MPQSFTSIARIGDYILKSPVLSKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTAL Sbjct: 1 MPQSFTSIARIGDYILKSPVLSKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTAL 60 Query: 61 RRLPEDESYARAYRIIRAHQTELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL 120 RRLPEDESYARAYRIIRAHQTELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL Sbjct: 61 RRLPEDESYARAYRIIRAHQTELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL 120 Query: 121 DNIEVSK 127 DNIEVSK Sbjct: 121 DNIEVSK 127 >1BCC-F nrPDB Length = 109 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 40/100 (40%), Positives = 57/100 (57%), Gaps = 1/100 (1%) Query: 22 SKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQT 81 S+ + + N AG+ K GL DD I E N ++ A+RRLPE+ R +RI RA Sbjct: 10 SRWLEGIRKWYYNAAGFNKYGLMRDDTIYE-NDDVKEAIRRLPENLYDDRMFRIKRALDL 68 Query: 82 ELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDELD 121 + +LP+ +W K +EDVPYL PY+ E KE++E D Sbjct: 69 NMRQQILPKEQWTKYEEDVPYLEPYLKEVIRERKEREEWD 108 >3CWB-F nrPDB Length = 110 Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 35/88 (39%), Positives = 53/88 (60%), Gaps = 1/88 (1%) Query: 22 SKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQT 81 +L + + N AG+ K GL DD + E++ + + AL+RLP+D R +RI RA Sbjct: 10 GRLMDRIRKWYYNAAGFNKYGLMRDDTLYEDDDV-KEALKRLPKDLYNERMFRIKRALDL 68 Query: 82 ELTHHLLPRNEWIKAQEDVPYLLPYILE 109 L H +LP+ +W+K +ED PYL PY+ E Sbjct: 69 SLKHRILPKEQWVKYEEDKPYLEPYLKE 96 >4D6U-F nrPDB Length = 111 Score = 68.6 bits (166), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 38/99 (38%), Positives = 55/99 (55%), Gaps = 1/99 (1%) Query: 22 SKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQT 81 S+ + + N AG+ KLGL DD I EN ++ A+RRLPE+ R +RI RA Sbjct: 11 SRWLEGIRKWYYNAAGFNKLGLMRDDTI-HENDDVKEAIRRLPENLYDDRVFRIKRALDL 69 Query: 82 ELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL 120 + +LP+ +W K +ED YL PY+ E KE++E Sbjct: 70 SMRQQILPKEQWTKYEEDKSYLEPYLKEVIRERKEREEW 108 >5XTI-AF nrPDB Length = 106 Score = 68.2 bits (165), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 38/98 (38%), Positives = 55/98 (56%), Gaps = 1/98 (1%) Query: 22 SKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQT 81 K + + N AG+ KLGL DD I E+ + + A+RRLPE+ R +RI RA Sbjct: 6 GKWLDGIRKWYYNAAGFNKLGLMRDDTIYEDEDV-KEAIRRLPENLYNDRMFRIKRALDL 64 Query: 82 ELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDE 119 L H +LP+ +W K +E+ YL PY+ E KE++E Sbjct: 65 NLKHQILPKEQWTKYEEENFYLEPYLKEVIRERKEREE 102 >6HAW-F nrPDB Length = 99 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 37/93 (39%), Positives = 53/93 (56%), Gaps = 1/93 (1%) Query: 28 VANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQTELTHHL 87 + + N AG+ KLGL DD I EN ++ A+RRLPE+ R +RI RA + + Sbjct: 6 IRKWYYNAAGFNKLGLMRDDTI-HENDDVKEAIRRLPENLYDDRVFRIKRALDLSMRQQI 64 Query: 88 LPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL 120 LP+ +W K +ED YL PY+ E KE++E Sbjct: 65 LPKEQWTKYEEDKSYLEPYLKEVIRERKEREEW 97 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6GIQ-G nrPDB 197 1e-51 1BCC-F nrPDB 155 4e-39 4D6U-F nrPDB 152 4e-38 5XTI-AF nrPDB 150 1e-37 6HAW-F nrPDB 146 2e-36 3CWB-F nrPDB 144 1e-35 Sequences not found previously or not previously below threshold: 3CIN-A nrPDB 27 2.3 2VNA-A nrPDB 27 2.5 2BBJ-A nrPDB 27 2.9 2Z5F-A nrPDB 26 3.8 5NAV-A nrPDB 26 4.2 5LOY-A nrPDB 26 4.6 2J8G-A nrPDB 26 5.2 2BBH-A nrPDB 25 6.2 1Z47-A nrPDB 25 6.2 3GC9-A nrPDB 25 7.0 4B68-A nrPDB 25 7.8 5OFQ-A nrPDB 25 9.1 CONVERGED! >6GIQ-G nrPDB Length = 127 Score = 197 bits (500), Expect = 1e-51, Method: Composition-based stats. Identities = 127/127 (100%), Positives = 127/127 (100%) Query: 1 MPQSFTSIARIGDYILKSPVLSKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTAL 60 MPQSFTSIARIGDYILKSPVLSKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTAL Sbjct: 1 MPQSFTSIARIGDYILKSPVLSKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTAL 60 Query: 61 RRLPEDESYARAYRIIRAHQTELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL 120 RRLPEDESYARAYRIIRAHQTELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL Sbjct: 61 RRLPEDESYARAYRIIRAHQTELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL 120 Query: 121 DNIEVSK 127 DNIEVSK Sbjct: 121 DNIEVSK 127 >1BCC-F nrPDB Length = 109 Score = 155 bits (392), Expect = 4e-39, Method: Composition-based stats. Identities = 40/100 (40%), Positives = 57/100 (57%), Gaps = 1/100 (1%) Query: 22 SKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQT 81 S+ + + N AG+ K GL DD I E N ++ A+RRLPE+ R +RI RA Sbjct: 10 SRWLEGIRKWYYNAAGFNKYGLMRDDTIYE-NDDVKEAIRRLPENLYDDRMFRIKRALDL 68 Query: 82 ELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDELD 121 + +LP+ +W K +EDVPYL PY+ E KE++E D Sbjct: 69 NMRQQILPKEQWTKYEEDVPYLEPYLKEVIRERKEREEWD 108 >4D6U-F nrPDB Length = 111 Score = 152 bits (384), Expect = 4e-38, Method: Composition-based stats. Identities = 38/99 (38%), Positives = 55/99 (55%), Gaps = 1/99 (1%) Query: 22 SKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQT 81 S+ + + N AG+ KLGL DD I EN ++ A+RRLPE+ R +RI RA Sbjct: 11 SRWLEGIRKWYYNAAGFNKLGLMRDDTI-HENDDVKEAIRRLPENLYDDRVFRIKRALDL 69 Query: 82 ELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL 120 + +LP+ +W K +ED YL PY+ E KE++E Sbjct: 70 SMRQQILPKEQWTKYEEDKSYLEPYLKEVIRERKEREEW 108 >5XTI-AF nrPDB Length = 106 Score = 150 bits (379), Expect = 1e-37, Method: Composition-based stats. Identities = 38/99 (38%), Positives = 55/99 (55%), Gaps = 1/99 (1%) Query: 22 SKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQT 81 K + + N AG+ KLGL DD I E+ ++ A+RRLPE+ R +RI RA Sbjct: 6 GKWLDGIRKWYYNAAGFNKLGLMRDDTIYEDED-VKEAIRRLPENLYNDRMFRIKRALDL 64 Query: 82 ELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL 120 L H +LP+ +W K +E+ YL PY+ E KE++E Sbjct: 65 NLKHQILPKEQWTKYEEENFYLEPYLKEVIRERKEREEW 103 >6HAW-F nrPDB Length = 99 Score = 146 bits (369), Expect = 2e-36, Method: Composition-based stats. Identities = 37/98 (37%), Positives = 54/98 (55%), Gaps = 1/98 (1%) Query: 23 KLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQTE 82 + + + N AG+ KLGL DD I EN ++ A+RRLPE+ R +RI RA Sbjct: 1 RWLEGIRKWYYNAAGFNKLGLMRDDTI-HENDDVKEAIRRLPENLYDDRVFRIKRALDLS 59 Query: 83 LTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDEL 120 + +LP+ +W K +ED YL PY+ E KE++E Sbjct: 60 MRQQILPKEQWTKYEEDKSYLEPYLKEVIRERKEREEW 97 >3CWB-F nrPDB Length = 110 Score = 144 bits (363), Expect = 1e-35, Method: Composition-based stats. Identities = 36/100 (36%), Positives = 56/100 (56%), Gaps = 1/100 (1%) Query: 22 SKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQT 81 +L + + N AG+ K GL DD + E+ ++ AL+RLP+D R +RI RA Sbjct: 10 GRLMDRIRKWYYNAAGFNKYGLMRDDTLYED-DDVKEALKRLPKDLYNERMFRIKRALDL 68 Query: 82 ELTHHLLPRNEWIKAQEDVPYLLPYILEAEAAAKEKDELD 121 L H +LP+ +W+K +ED PYL PY+ E E++ + Sbjct: 69 SLKHRILPKEQWVKYEEDKPYLEPYLKEVIRERLEREAWN 108 >3CIN-A nrPDB Length = 394 Score = 26.9 bits (58), Expect = 2.3, Method: Composition-based stats. Identities = 22/105 (20%), Positives = 36/105 (34%), Gaps = 22/105 (20%) Query: 1 MPQSFTSIARIGDYILKSPVLSKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQ--- 57 +P F I +G Y + + K V Q+ N D + + I + Sbjct: 51 LPIGFEDIKIVGSYDVDRAKIGKKLSEVVKQYWNDV----------DSLTSDPEIRKGVH 100 Query: 58 -TALRRLP-EDESYARAYRIIRAHQTELTHHLLPRNEWIKAQEDV 100 ++R LP E E + + A T + EW + DV Sbjct: 101 LGSVRNLPIEAEGLEDSMTLKEAVDTLVK-------EWTELDPDV 138 >2VNA-A nrPDB Length = 357 Score = 26.9 bits (58), Expect = 2.5, Method: Composition-based stats. Identities = 19/82 (23%), Positives = 29/82 (35%), Gaps = 6/82 (7%) Query: 43 LKFDDLIAEENPIMQTALRR-LPE--DESYARA---YRIIRAHQTELTHHLLPRNEWIKA 96 L FD I + + LR P D + Q H++ + + Sbjct: 201 LGFDAAINYKKDNVAEQLRESCPAGVDVYFDNVGGNISDTVISQMNENSHIILCGQISQY 260 Query: 97 QEDVPYLLPYILEAEAAAKEKD 118 +DVPY P EA KE++ Sbjct: 261 NKDVPYPPPLSPAIEAIQKERN 282 >2BBJ-A nrPDB Length = 354 Score = 26.5 bits (57), Expect = 2.9, Method: Composition-based stats. Identities = 17/70 (24%), Positives = 30/70 (42%), Gaps = 7/70 (10%) Query: 47 DLIAEENPIMQTALRRLPEDESYARAYRIIRAHQTELTHHLLP-RNEWIKAQEDVPYLL- 104 + I +E +++ + PE E+ R +++ R EL + P R DVP L+ Sbjct: 189 EKIDDEIDVLEEEVLERPEKETVQRTHQLKRNL-VELRKTIWPLREVLSSLYRDVPPLIE 247 Query: 105 ----PYILEA 110 PY + Sbjct: 248 KETVPYFRDV 257 >2Z5F-A nrPDB Length = 298 Score = 26.2 bits (56), Expect = 3.8, Method: Composition-based stats. Identities = 10/55 (18%), Positives = 21/55 (38%) Query: 22 SKLCVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRII 76 V N + + L L ++D+ ++ +R L ++ + RII Sbjct: 173 GSWFTHVKNWWKRKEEHPILFLYYEDMKENPKEEIKKIIRFLEKNLNDEILDRII 227 >5NAV-A nrPDB Length = 477 Score = 26.2 bits (56), Expect = 4.2, Method: Composition-based stats. Identities = 6/33 (18%), Positives = 15/33 (45%) Query: 27 PVANQFINLAGYKKLGLKFDDLIAEENPIMQTA 59 + N A + + GL +D+ + + ++A Sbjct: 433 GIDCWSWNHAYHNRYGLIRNDIHTQTKTLKKSA 465 >5LOY-A nrPDB Length = 240 Score = 25.8 bits (55), Expect = 4.6, Method: Composition-based stats. Identities = 15/53 (28%), Positives = 22/53 (41%) Query: 41 LGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQTELTHHLLPRNEW 93 +GL D L+ E + + RR+ ED+ Y R R + LP W Sbjct: 187 VGLPLDLLVYERDSLRVGHRRRIDEDDPYFRMLRKQWSEGLRQAFDSLPDPPW 239 >2J8G-A nrPDB Length = 339 Score = 25.8 bits (55), Expect = 5.2, Method: Composition-based stats. Identities = 7/35 (20%), Positives = 13/35 (37%), Gaps = 3/35 (8%) Query: 25 CVPVANQFINLAGYKKLGLKFDDLIAEENPIMQTA 59 + + + K + DD EE+ +TA Sbjct: 168 MDGIRWWQYSSNPFDKNIVLLDD---EEDDKPKTA 199 >2BBH-A nrPDB Length = 269 Score = 25.4 bits (54), Expect = 6.2, Method: Composition-based stats. Identities = 17/70 (24%), Positives = 30/70 (42%), Gaps = 7/70 (10%) Query: 47 DLIAEENPIMQTALRRLPEDESYARAYRIIRAHQTELTHHLLP-RNEWIKAQEDVPYLL- 104 + I +E +++ + PE E+ R +++ R EL + P R DVP L+ Sbjct: 189 EKIDDEIDVLEEEVLERPEKETVQRTHQLKRNL-VELRKTIWPLREVLSSLYRDVPPLIE 247 Query: 105 ----PYILEA 110 PY + Sbjct: 248 KETVPYFRDV 257 >1Z47-A nrPDB Length = 355 Score = 25.4 bits (54), Expect = 6.2, Method: Composition-based stats. Identities = 11/54 (20%), Positives = 22/54 (40%), Gaps = 1/54 (1%) Query: 38 YKKLGLKFDDLIAEENPIMQTALRRLPEDESYARAYRIIRAHQTELTHHLLPRN 91 ++ L + +N +R+P+DE AR ++R + E + P Sbjct: 93 FQNYALFQHMTVY-DNVSFGLREKRVPKDEMDARVRELLRFMRLESYANRFPHE 145 >3GC9-A nrPDB Length = 370 Score = 25.4 bits (54), Expect = 7.0, Method: Composition-based stats. Identities = 8/26 (30%), Positives = 11/26 (42%) Query: 98 EDVPYLLPYILEAEAAAKEKDELDNI 123 ED P PY EA + +E + Sbjct: 321 EDEPEAEPYDESVEAKERTLEEWKEL 346 >4B68-A nrPDB Length = 501 Score = 25.0 bits (53), Expect = 7.8, Method: Composition-based stats. Identities = 8/40 (20%), Positives = 16/40 (40%), Gaps = 1/40 (2%) Query: 63 LPEDESYARAY-RIIRAHQTELTHHLLPRNEWIKAQEDVP 101 L E+ R+ +T+ H +LP + + + P Sbjct: 331 LIEEIYNDMYLQRVKNPDETQWQHRILPERKITRVEHHGP 370 >5OFQ-A nrPDB Length = 409 Score = 25.0 bits (53), Expect = 9.1, Method: Composition-based stats. Identities = 9/31 (29%), Positives = 14/31 (45%) Query: 95 KAQEDVPYLLPYILEAEAAAKEKDELDNIEV 125 K E V +L Y E A ++ + D I + Sbjct: 185 KRNEGVIFLQGYFKEIIAERQQNKQEDLISL 215 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.135 0.386 Lambda K H 0.267 0.0417 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,204,270 Number of Sequences: 36641 Number of extensions: 194757 Number of successful extensions: 504 Number of sequences better than 10.0: 27 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 16 Number of HSP's that attempted gapping in prelim test: 475 Number of HSP's gapped (non-prelim): 35 length of query: 127 length of database: 10,336,785 effective HSP length: 86 effective length of query: 41 effective length of database: 7,185,659 effective search space: 294612019 effective search space used: 294612019 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 53 (25.0 bits)