BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O96033 (88 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5MPO-A nrPDB 166 3e-42 2QIE-B nrPDB 49 5e-07 1FM0-D nrPDB 35 0.006 2L83-A nrPDB 31 0.13 6IRV-A nrPDB 28 0.71 3C51-A nrPDB 28 0.84 4P1T-A nrPDB 28 0.94 4WSS-A nrPDB 27 2.7 4UM8-B nrPDB 25 7.5 4UM9-D nrPDB 25 8.6 >5MPO-A nrPDB Length = 106 Score = 166 bits (419), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 82/84 (97%), Positives = 82/84 (97%) Query: 5 CQVEVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYV 64 VEVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYV Sbjct: 23 SMVEVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYV 82 Query: 65 ELGDQLLVLQPGDEIAVIPPISGG 88 ELGDQLLVLQPGDEIAVIPPISGG Sbjct: 83 ELGDQLLVLQPGDEIAVIPPISGG 106 >2QIE-B nrPDB Length = 77 Score = 48.9 bits (115), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 28/82 (34%), Positives = 47/82 (57%), Gaps = 5/82 (6%) Query: 7 VEVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVEL 66 ++VLYFA+ +I E I + Q + Q + R+P + + + Q+ AV +E+V+ Sbjct: 1 MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINNKKFQV--AVNEEFVQK 58 Query: 67 GDQLLVLQPGDEIAVIPPISGG 88 D +QP D +A+IPP+SGG Sbjct: 59 SD---FIQPNDTVALIPPVSGG 77 >1FM0-D nrPDB Length = 81 Score = 35.4 bits (80), Expect = 0.006, Method: Compositional matrix adjust. Identities = 23/83 (27%), Positives = 42/83 (50%), Gaps = 4/83 (4%) Query: 7 VEVLYFAKSAEITGVRSETISVP-QEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVE 65 ++VL+FA+ E+ G + ++ ++AL+ ++ LA +++ AV Q V Sbjct: 2 IKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWALALEDGKLLAAVNQTLVS 61 Query: 66 LGDQLLVLQPGDEIAVIPPISGG 88 L GDE+A PP++GG Sbjct: 62 FDHPL---TDGDEVAFFPPVTGG 81 >2L83-A nrPDB Length = 95 Score = 30.8 bits (68), Expect = 0.13, Method: Compositional matrix adjust. Identities = 25/89 (28%), Positives = 37/89 (41%), Gaps = 19/89 (21%) Query: 12 FAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVELGDQLL 71 FA AE+ G R+ + V + + HP L +F + EL D + Sbjct: 6 FADLAEVAGSRTVRVDVDGDATVGDALDALVGAHPAL----ESRVFG---DDGELYDHIN 58 Query: 72 VLQPG------------DEIAVIPPISGG 88 VL+ G DE+A+ PP+SGG Sbjct: 59 VLRNGEAAALGEATAAGDELALFPPVSGG 87 >6IRV-A nrPDB Length = 508 Score = 28.5 bits (62), Expect = 0.71, Method: Composition-based stats. Identities = 14/39 (35%), Positives = 22/39 (56%) Query: 40 EIETRHPGLADVRNQIIFAVRQEYVELGDQLLVLQPGDE 78 E+ HP + +R+Q+I +RQ Y EL Q ++P E Sbjct: 29 EVLPPHPEVELLRSQLILKLRQHYRELCQQREGIEPPRE 67 >3C51-A nrPDB Length = 543 Score = 28.1 bits (61), Expect = 0.84, Method: Composition-based stats. Identities = 17/42 (40%), Positives = 21/42 (50%) Query: 30 QEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVELGDQLL 71 Q ALQLWK+IE +R Q A+R Y+E QL Sbjct: 81 QHGPALQLWKDIEDYDTADDALRPQKAQALRAAYLEPQAQLF 122 >4P1T-A nrPDB Length = 746 Score = 28.1 bits (61), Expect = 0.94, Method: Composition-based stats. Identities = 13/45 (28%), Positives = 22/45 (48%) Query: 24 ETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVELGD 68 ET+ + E +A LWK+ G+ D + A+R + +L D Sbjct: 446 ETLQIVAEREAYYLWKQYNPTGKGIDDANKKACCAIRGSFYDLED 490 >4WSS-A nrPDB Length = 514 Score = 26.6 bits (57), Expect = 2.7, Method: Composition-based stats. Identities = 12/38 (31%), Positives = 19/38 (50%) Query: 50 DVRNQIIFAVRQEYVELGDQLLVLQPGDEIAVIPPISG 87 DV ++ + YV +G + + G EIA PP +G Sbjct: 189 DVEQANLYGLGTRYVRMGTESMNFAKGPEIADRPPANG 226 >4UM8-B nrPDB Length = 788 Score = 25.0 bits (53), Expect = 7.5, Method: Composition-based stats. Identities = 13/33 (39%), Positives = 18/33 (54%) Query: 55 IIFAVRQEYVELGDQLLVLQPGDEIAVIPPISG 87 +IFAV QE V L + L PG + ++ SG Sbjct: 326 LIFAVTQEQVHLYENYAKLIPGATVGLLQKDSG 358 >4UM9-D nrPDB Length = 483 Score = 24.6 bits (52), Expect = 8.6, Method: Composition-based stats. Identities = 13/33 (39%), Positives = 18/33 (54%) Query: 55 IIFAVRQEYVELGDQLLVLQPGDEIAVIPPISG 87 +IFAV QE V L + L PG + ++ SG Sbjct: 309 LIFAVTQEQVHLYENYAKLIPGATVGLLQKDSG 341 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5MPO-A nrPDB 135 4e-33 2QIE-B nrPDB 99 4e-22 Sequences not found previously or not previously below threshold: 1FM0-D nrPDB 37 0.002 2L83-A nrPDB 33 0.029 4YJI-A nrPDB 28 0.77 1V8C-A nrPDB 28 1.3 6IQ5-A nrPDB 27 1.8 6IRV-A nrPDB 27 2.3 4J8P-A nrPDB 27 2.3 1JWK-A nrPDB 26 3.8 1F6B-A nrPDB 26 4.4 5T13-A nrPDB 25 5.4 1GFS-A nrPDB 25 6.4 >5MPO-A nrPDB Length = 106 Score = 135 bits (340), Expect = 4e-33, Method: Composition-based stats. Identities = 82/84 (97%), Positives = 82/84 (97%) Query: 5 CQVEVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYV 64 VEVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYV Sbjct: 23 SMVEVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYV 82 Query: 65 ELGDQLLVLQPGDEIAVIPPISGG 88 ELGDQLLVLQPGDEIAVIPPISGG Sbjct: 83 ELGDQLLVLQPGDEIAVIPPISGG 106 >2QIE-B nrPDB Length = 77 Score = 99.0 bits (245), Expect = 4e-22, Method: Composition-based stats. Identities = 28/82 (34%), Positives = 47/82 (57%), Gaps = 5/82 (6%) Query: 7 VEVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVEL 66 ++VLYFA+ +I E I + Q + Q + R+P + + + Q+ AV +E+V+ Sbjct: 1 MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINNKKFQV--AVNEEFVQK 58 Query: 67 GDQLLVLQPGDEIAVIPPISGG 88 D +QP D +A+IPP+SGG Sbjct: 59 SD---FIQPNDTVALIPPVSGG 77 >1FM0-D nrPDB Length = 81 Score = 37.0 bits (84), Expect = 0.002, Method: Composition-based stats. Identities = 23/84 (27%), Positives = 42/84 (50%), Gaps = 4/84 (4%) Query: 6 QVEVLYFAKSAEITGVRSETISVP-QEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYV 64 ++VL+FA+ E+ G + ++ ++AL+ ++ LA +++ AV Q V Sbjct: 1 MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWALALEDGKLLAAVNQTLV 60 Query: 65 ELGDQLLVLQPGDEIAVIPPISGG 88 L GDE+A PP++GG Sbjct: 61 SFDHP---LTDGDEVAFFPPVTGG 81 >2L83-A nrPDB Length = 95 Score = 33.2 bits (74), Expect = 0.029, Method: Composition-based stats. Identities = 25/94 (26%), Positives = 37/94 (39%), Gaps = 19/94 (20%) Query: 7 VEVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVEL 66 +E FA AE+ G R+ + V + + HP L + EL Sbjct: 1 MEWKLFADLAEVAGSRTVRVDVDGDATVGDALDALVGAHPALESRVFG-------DDGEL 53 Query: 67 GDQLLVLQPG------------DEIAVIPPISGG 88 D + VL+ G DE+A+ PP+SGG Sbjct: 54 YDHINVLRNGEAAALGEATAAGDELALFPPVSGG 87 >4YJI-A nrPDB Length = 495 Score = 28.1 bits (61), Expect = 0.77, Method: Composition-based stats. Identities = 17/76 (22%), Positives = 34/76 (44%), Gaps = 1/76 (1%) Query: 10 LYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVELGDQ 69 +++ +AEI + P+E+ IE R PGL V + R++ L + Sbjct: 8 VHWKSAAEIVELVKSKQISPREV-VESTIDLIEQRDPGLNAVVYKAYDEAREKAAALERR 66 Query: 70 LLVLQPGDEIAVIPPI 85 ++ +P +A +P + Sbjct: 67 IMQGEPVGMLAGVPTL 82 >1V8C-A nrPDB Length = 168 Score = 27.8 bits (60), Expect = 1.3, Method: Composition-based stats. Identities = 19/89 (21%), Positives = 34/89 (38%), Gaps = 11/89 (12%) Query: 8 EVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQ-IIFAVRQEYVEL 66 +V +A ++TG + +P L + +P L + + A R Sbjct: 2 KVNLYATFRDLTGKSQ--LELPGATVGEVLENLVRA-YPALKEELFEGEGLAERVSVFLE 58 Query: 67 GDQLLVLQ-------PGDEIAVIPPISGG 88 G + LQ PG + + PP++GG Sbjct: 59 GRDVRYLQGLSTPLSPGATLDLFPPVAGG 87 >6IQ5-A nrPDB Length = 463 Score = 27.0 bits (58), Expect = 1.8, Method: Composition-based stats. Identities = 11/36 (30%), Positives = 17/36 (47%), Gaps = 5/36 (13%) Query: 13 AKSAEITGVRSETISVPQEIKALQLWKEIETRHPGL 48 A +I G +T+S ALQ + TR+P + Sbjct: 255 ATITDIFGASQDTLS-----TALQWLLLLFTRYPDV 285 >6IRV-A nrPDB Length = 508 Score = 26.6 bits (57), Expect = 2.3, Method: Composition-based stats. Identities = 13/36 (36%), Positives = 21/36 (58%) Query: 40 EIETRHPGLADVRNQIIFAVRQEYVELGDQLLVLQP 75 E+ HP + +R+Q+I +RQ Y EL Q ++P Sbjct: 29 EVLPPHPEVELLRSQLILKLRQHYRELCQQREGIEP 64 >4J8P-A nrPDB Length = 159 Score = 26.6 bits (57), Expect = 2.3, Method: Composition-based stats. Identities = 10/54 (18%), Positives = 23/54 (42%) Query: 12 FAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVE 65 F +S + G + KE++T +PG+ + +++ +E V+ Sbjct: 101 FIESGDFAGKTVIPFATSGSSSISNAEKELQTNYPGINWGKGRLLNGASRETVK 154 >1JWK-A nrPDB Length = 434 Score = 25.8 bits (55), Expect = 3.8, Method: Composition-based stats. Identities = 8/13 (61%), Positives = 11/13 (84%) Query: 75 PGDEIAVIPPISG 87 P D IA++PP+SG Sbjct: 388 PADWIALVPPVSG 400 >1F6B-A nrPDB Length = 198 Score = 25.8 bits (55), Expect = 4.4, Method: Composition-based stats. Identities = 15/71 (21%), Positives = 31/71 (43%), Gaps = 3/71 (4%) Query: 13 AKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVELGDQLLV 72 ++ I G+ T + I+A ++WK P + + + A + +E ++L Sbjct: 61 SEELTIAGMTFTTFDLGGHIQARRVWKNYL---PAINGIVFLVDCADHERLLESKEELDS 117 Query: 73 LQPGDEIAVIP 83 L + IA +P Sbjct: 118 LMTDETIANVP 128 >5T13-A nrPDB Length = 376 Score = 25.4 bits (54), Expect = 5.4, Method: Composition-based stats. Identities = 14/74 (18%), Positives = 31/74 (41%), Gaps = 1/74 (1%) Query: 1 MVPLCQVEVLYFAKSAEITGVRSETISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVR 60 M P C + + + + T + ++V Q L +EI R P + + + + A+R Sbjct: 95 MAPHCTIFTVQKTDNKQKTAAEGKRLAVQQIFTREFLPEEI-GRMPQVTETADAVRRAMR 153 Query: 61 QEYVELGDQLLVLQ 74 + + + +Q Sbjct: 154 EAGIADASDVHFVQ 167 >1GFS-A nrPDB Length = 321 Score = 25.1 bits (53), Expect = 6.4, Method: Composition-based stats. Identities = 11/35 (31%), Positives = 21/35 (60%) Query: 48 LADVRNQIIFAVRQEYVELGDQLLVLQPGDEIAVI 82 +A R + A+R++ + GD LVL+ DE+ ++ Sbjct: 8 IAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLL 42 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.130 0.349 Lambda K H 0.267 0.0385 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 2,829,442 Number of Sequences: 36641 Number of extensions: 77884 Number of successful extensions: 298 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 274 Number of HSP's gapped (non-prelim): 27 length of query: 88 length of database: 10,336,785 effective HSP length: 57 effective length of query: 31 effective length of database: 8,248,248 effective search space: 255695688 effective search space used: 255695688 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 52 (24.7 bits)