BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O95863 (264 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3W5K-B nrPDB 541 e-155 2EBT-A nrPDB 60 1e-09 3ZMS-C nrPDB 31 0.44 5VAZ-A nrPDB 31 0.71 4CFG-A nrPDB 28 3.0 5APG-A nrPDB 28 5.3 5GL7-A nrPDB 28 5.8 4C0T-A nrPDB 27 7.5 >3W5K-B nrPDB Length = 264 Score = 541 bits (1395), Expect = e-155, Method: Compositional matrix adjust. Identities = 264/264 (100%), Positives = 264/264 (100%) Query: 1 MPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLI 60 MPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLI Sbjct: 1 MPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLI 60 Query: 61 WDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSS 120 WDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSS Sbjct: 61 WDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSS 120 Query: 121 LEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYLSLGALKMHIRSHTLPC 180 LEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYLSLGALKMHIRSHTLPC Sbjct: 121 LEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYLSLGALKMHIRSHTLPC 180 Query: 181 VCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQA 240 VCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQA Sbjct: 181 VCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQA 240 Query: 241 CARTFSRMSLLHKHQESGCSGCPR 264 CARTFSRMSLLHKHQESGCSGCPR Sbjct: 241 CARTFSRMSLLHKHQESGCSGCPR 264 >2EBT-A nrPDB Length = 100 Score = 59.7 bits (143), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 34/92 (36%), Positives = 46/92 (50%), Gaps = 6/92 (6%) Query: 147 DLQARKAFNCKY--CNKEYLSLGALKMHIRSHT----LPCVCGTCGKAFSRPWLLQGHVR 200 DL+ R+ C Y C K Y LK H+R+HT C C F+R L H R Sbjct: 9 DLEKRRIHYCDYPGCTKVYTKSSHLKAHLRTHTGEKPYKCTWEGCDWRFARSDELTRHYR 68 Query: 201 THTGEKPFSCPHCSRAFADRSNLRAHLQTHSD 232 HTG KPF C C+R+F+ +L H++ H + Sbjct: 69 KHTGAKPFQCGVCNRSFSRSDHLALHMKRHQN 100 Score = 55.8 bits (133), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 28/69 (40%), Positives = 38/69 (55%), Gaps = 2/69 (2%) Query: 185 CGKAFSRPWLLQGHVRTHTGEKPFSCP--HCSRAFADRSNLRAHLQTHSDVKKYQCQACA 242 C K +++ L+ H+RTHTGEKP+ C C FA L H + H+ K +QC C Sbjct: 23 CTKVYTKSSHLKAHLRTHTGEKPYKCTWEGCDWRFARSDELTRHYRKHTGAKPFQCGVCN 82 Query: 243 RTFSRMSLL 251 R+FSR L Sbjct: 83 RSFSRSDHL 91 Score = 35.4 bits (80), Expect = 0.027, Method: Compositional matrix adjust. Identities = 17/46 (36%), Positives = 28/46 (60%), Gaps = 2/46 (4%) Query: 211 PHCSRAFADRSNLRAHLQTHSDVKKYQC--QACARTFSRMSLLHKH 254 P C++ + S+L+AHL+TH+ K Y+C + C F+R L +H Sbjct: 21 PGCTKVYTKSSHLKAHLRTHTGEKPYKCTWEGCDWRFARSDELTRH 66 >3ZMS-C nrPDB Length = 509 Score = 31.2 bits (69), Expect = 0.44, Method: Compositional matrix adjust. Identities = 23/118 (19%), Positives = 40/118 (33%), Gaps = 44/118 (37%) Query: 181 VCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTH---------- 230 +C C + ++ P+ L H + + CP C++ F+ +NL +H + H Sbjct: 267 ICQLCKEEYADPFALAQHKCSRIVRVEYRCPECAKVFSCPANLASHRRWHKPRPAPAAAR 326 Query: 231 ----------------------------------SDVKKYQCQACARTFSRMSLLHKH 254 S+ Y+C CA+ F R + L KH Sbjct: 327 APEPEAAARAEAREAPGGGSDRDTPSPGGVSESGSEDGLYECHHCAKKFRRQAYLRKH 384 Score = 27.3 bits (59), Expect = 7.2, Method: Compositional matrix adjust. Identities = 13/45 (28%), Positives = 20/45 (44%) Query: 210 CPHCSRAFADRSNLRAHLQTHSDVKKYQCQACARTFSRMSLLHKH 254 CP C +FA + HL+ + + C+ C TF L +H Sbjct: 442 CPVCGESFASKGAQERHLRLLHAAQVFPCKYCPATFYSSPGLTRH 486 >5VAZ-A nrPDB Length = 389 Score = 30.8 bits (68), Expect = 0.71, Method: Compositional matrix adjust. Identities = 31/101 (30%), Positives = 44/101 (43%), Gaps = 16/101 (15%) Query: 28 EFTFQQPYDQAHLLAAIPPPEILNPTASLPMLIWDSVLAPQAQPIAWASLRLQESPRVAE 87 E+ FQQ +A P L A L L AP + I +LRL R++E Sbjct: 277 EYFFQQLMLEAD-------PATLEGKAHLATLA-----APLLEKIPGNNLRLLMRQRLSE 324 Query: 88 LTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSSLEAEAYAA 128 +T LS E+ G+ + SP PSS + L+ + Y A Sbjct: 325 ITGLSGENIGQLAH----HSPPPSSMDHGASGVLDGDDYFA 361 >4CFG-A nrPDB Length = 630 Score = 28.5 bits (62), Expect = 3.0, Method: Compositional matrix adjust. Identities = 14/47 (29%), Positives = 18/47 (38%), Gaps = 8/47 (17%) Query: 177 TLPCVCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNL 223 T PC CG + W +QG P+ CP C + R L Sbjct: 25 TTPCGHNFCGSCLNETWAVQG--------SPYLCPQCRAVYQARPQL 63 >5APG-A nrPDB Length = 185 Score = 27.7 bits (60), Expect = 5.3, Method: Compositional matrix adjust. Identities = 23/67 (34%), Positives = 31/67 (46%), Gaps = 8/67 (11%) Query: 1 MPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPE-ILNPTASLPML 59 +PR F+ R P D RK +L F F Q D + A+P IL+PT P+ Sbjct: 2 IPRVFIYRLPQDDPRKNTAIKLV----RFGFAQLVDS---IKALPSGSIILDPTVKTPLT 54 Query: 60 IWDSVLA 66 D V+A Sbjct: 55 PSDRVIA 61 >5GL7-A nrPDB Length = 624 Score = 27.7 bits (60), Expect = 5.8, Method: Compositional matrix adjust. Identities = 21/71 (29%), Positives = 30/71 (42%), Gaps = 3/71 (4%) Query: 194 LLQGHVRTHTGEKPFSCPHCSRAFADR-SNLRAHLQTHSDVKKYQCQACARTFSRMSLLH 252 L +G V T E+ C HC+R ADR + + + QC C + + + L Sbjct: 156 LKRGFVLQDTVEQ-LRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVE-LK 213 Query: 253 KHQESGCSGCP 263 K Q C CP Sbjct: 214 KPQCKVCRSCP 224 >4C0T-A nrPDB Length = 974 Score = 27.3 bits (59), Expect = 7.5, Method: Composition-based stats. Identities = 13/45 (28%), Positives = 22/45 (48%) Query: 85 VAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSSLEAEAYAAF 129 VA S + KGS P P+P++ ++ S + A + AA+ Sbjct: 591 VATAVGGSSSNGHKGSSPTPEKEPSPATINNKSTEKVSAASVAAY 635 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3W5K-B nrPDB 472 e-134 2EBT-A nrPDB 149 2e-36 Sequences not found previously or not previously below threshold: 5VAZ-A nrPDB 35 0.042 3ZMS-C nrPDB 33 0.13 5NUS-A nrPDB 32 0.22 4R7E-A nrPDB 30 1.4 4C0T-A nrPDB 29 2.5 3FLO-B nrPDB 29 2.9 1B8T-A nrPDB 28 5.8 6BJC-T nrPDB 27 6.5 CONVERGED! >3W5K-B nrPDB Length = 264 Score = 472 bits (1214), Expect = e-134, Method: Composition-based stats. Identities = 264/264 (100%), Positives = 264/264 (100%) Query: 1 MPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLI 60 MPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLI Sbjct: 1 MPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEILNPTASLPMLI 60 Query: 61 WDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSS 120 WDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSS Sbjct: 61 WDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSS 120 Query: 121 LEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYLSLGALKMHIRSHTLPC 180 LEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYLSLGALKMHIRSHTLPC Sbjct: 121 LEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCKYCNKEYLSLGALKMHIRSHTLPC 180 Query: 181 VCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQA 240 VCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQA Sbjct: 181 VCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQA 240 Query: 241 CARTFSRMSLLHKHQESGCSGCPR 264 CARTFSRMSLLHKHQESGCSGCPR Sbjct: 241 CARTFSRMSLLHKHQESGCSGCPR 264 >2EBT-A nrPDB Length = 100 Score = 149 bits (375), Expect = 2e-36, Method: Composition-based stats. Identities = 35/96 (36%), Positives = 47/96 (48%), Gaps = 6/96 (6%) Query: 143 SEAKDLQARKAFNCKY--CNKEYLSLGALKMHIRSHT----LPCVCGTCGKAFSRPWLLQ 196 S DL+ R+ C Y C K Y LK H+R+HT C C F+R L Sbjct: 5 SSGPDLEKRRIHYCDYPGCTKVYTKSSHLKAHLRTHTGEKPYKCTWEGCDWRFARSDELT 64 Query: 197 GHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSD 232 H R HTG KPF C C+R+F+ +L H++ H + Sbjct: 65 RHYRKHTGAKPFQCGVCNRSFSRSDHLALHMKRHQN 100 >5VAZ-A nrPDB Length = 389 Score = 34.7 bits (78), Expect = 0.042, Method: Composition-based stats. Identities = 33/108 (30%), Positives = 47/108 (43%), Gaps = 18/108 (16%) Query: 23 QDSNP--EFTFQQPYDQAHLLAAIPPPEILNPTASLPMLIWDSVLAPQAQPIAWASLRLQ 80 Q + P E+ FQQ +A P L A L L AP + I +LRL Sbjct: 270 QQAQPLAEYFFQQLMLEAD-------PATLEGKAHLATLA-----APLLEKIPGNNLRLL 317 Query: 81 ESPRVAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSSLEAEAYAA 128 R++E+T LS E+ G+ + SP PSS + L+ + Y A Sbjct: 318 MRQRLSEITGLSGENIGQLAH----HSPPPSSMDHGASGVLDGDDYFA 361 >3ZMS-C nrPDB Length = 509 Score = 33.2 bits (74), Expect = 0.13, Method: Composition-based stats. Identities = 23/122 (18%), Positives = 39/122 (31%), Gaps = 20/122 (16%) Query: 153 AFNCKYCNKEYLSLGALKMHIRSHTLPCVCGTCGKAFSRPWLLQ---------------- 196 + C +C K++ L+ H+ +H A LL Sbjct: 365 LYECHHCAKKFRRQAYLRKHLLAHHQALQAKGAPLAPPAEDLLALYPGPDEKAPQEAAGD 424 Query: 197 ----GHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQACARTFSRMSLLH 252 G + + CP C +FA + HL+ + + C+ C TF L Sbjct: 425 GEGAGVLGLSASAECHLCPVCGESFASKGAQERHLRLLHAAQVFPCKYCPATFYSSPGLT 484 Query: 253 KH 254 +H Sbjct: 485 RH 486 Score = 32.0 bits (71), Expect = 0.30, Method: Composition-based stats. Identities = 13/51 (25%), Positives = 26/51 (50%) Query: 181 VCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHS 231 +C C + ++ P+ L H + + CP C++ F+ +NL +H + H Sbjct: 267 ICQLCKEEYADPFALAQHKCSRIVRVEYRCPECAKVFSCPANLASHRRWHK 317 Score = 29.7 bits (65), Expect = 1.4, Method: Composition-based stats. Identities = 12/48 (25%), Positives = 20/48 (41%) Query: 182 CGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQT 229 C CG++F+ + H+R + F C +C F L H+ Sbjct: 442 CPVCGESFASKGAQERHLRLLHAAQVFPCKYCPATFYSSPGLTRHINK 489 >5NUS-A nrPDB Length = 303 Score = 32.4 bits (72), Expect = 0.22, Method: Composition-based stats. Identities = 12/37 (32%), Positives = 18/37 (48%) Query: 103 PSPPSPAPSSFSSTSVSSLEAEAYAAFPGLGQVPKQL 139 P PP P PS SS ++ ++ +P Q+ K L Sbjct: 109 PQPPEPLPSGSSSHDAAARKSATIGKYPQFAQIEKSL 145 >4R7E-A nrPDB Length = 69 Score = 29.7 bits (65), Expect = 1.4, Method: Composition-based stats. Identities = 18/77 (23%), Positives = 27/77 (35%), Gaps = 19/77 (24%) Query: 151 RKAFNCKYCNKEYLSLGALKMHIRSHTLPCVCGTCGKAFSRPWLLQGHVRTHTGEKPFSC 210 R C C+K + A+K TCG F + + + C Sbjct: 12 RTLVYCSLCSKNW-KNMAIK-------------TCGHVFC-----ENCCKERLAARMRKC 52 Query: 211 PHCSRAFADRSNLRAHL 227 P C++AF+ L HL Sbjct: 53 PTCNKAFSSNDLLTVHL 69 >4C0T-A nrPDB Length = 974 Score = 28.9 bits (63), Expect = 2.5, Method: Composition-based stats. Identities = 13/45 (28%), Positives = 22/45 (48%) Query: 85 VAELTSLSDEDSGKGSQPPSPPSPAPSSFSSTSVSSLEAEAYAAF 129 VA S + KGS P P+P++ ++ S + A + AA+ Sbjct: 591 VATAVGGSSSNGHKGSSPTPEKEPSPATINNKSTEKVSAASVAAY 635 >3FLO-B nrPDB Length = 206 Score = 28.5 bits (62), Expect = 2.9, Method: Composition-based stats. Identities = 18/101 (17%), Positives = 31/101 (30%), Gaps = 14/101 (13%) Query: 175 SHTLPCVCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAF-----------ADRSNL 223 + TL C +C K F ++ + C HC + F + R+++ Sbjct: 18 TVTLELSCPSCDKRFPFGGIVSSNYYR-VSYNGLQCKHCEQLFTPLQLTSQIEHSIRAHI 76 Query: 224 RAHLQTHSDVKKYQCQACARTFSRMSLLHKHQESGCSGCPR 264 + C R S + GC+G R Sbjct: 77 SLYYAGWLQCDDSTCGIVTRQVSVFG--KRCLNDGCTGVMR 115 >1B8T-A nrPDB Length = 192 Score = 27.8 bits (60), Expect = 5.8, Method: Composition-based stats. Identities = 15/54 (27%), Positives = 23/54 (42%), Gaps = 3/54 (5%) Query: 210 CPHCSRA-FADRSNLRAHLQTHSDVKKYQCQACARTFSRMSLLHKHQESGCSGC 262 CP C +A +A + A H ++C C ++ +L K E C GC Sbjct: 118 CPRCGQAVYAAEKVIGAGKSWHKSC--FRCAKCGKSLESTTLADKDGEIYCKGC 169 >6BJC-T nrPDB Length = 747 Score = 27.4 bits (59), Expect = 6.5, Method: Composition-based stats. Identities = 15/46 (32%), Positives = 24/46 (52%) Query: 112 SFSSTSVSSLEAEAYAAFPGLGQVPKQLAQLSEAKDLQARKAFNCK 157 S S + SSLE EA + Q ++ +LS KDL+ ++ + K Sbjct: 94 SIPSNACSSLEVEAAISRKTPAQPQRRSLRLSAQKDLEQKEKHHVK 139 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.130 0.396 Lambda K H 0.267 0.0399 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,743,359 Number of Sequences: 36641 Number of extensions: 425210 Number of successful extensions: 1283 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 1204 Number of HSP's gapped (non-prelim): 62 length of query: 264 length of database: 10,336,785 effective HSP length: 95 effective length of query: 169 effective length of database: 6,855,890 effective search space: 1158645410 effective search space used: 1158645410 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 58 (27.0 bits)