BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O95149 (360 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3NC0-B nrPDB 729 0.0 5DIS-C nrPDB 604 e-173 1XK5-A nrPDB 437 e-123 2QNA-B nrPDB 130 6e-31 4I6Z-A nrPDB 29 3.5 1QVJ-A nrPDB 28 6.7 >3NC0-B nrPDB Length = 362 Score = 729 bits (1883), Expect = 0.0, Method: Compositional matrix adjust. Identities = 346/349 (99%), Positives = 348/349 (99%) Query: 12 FSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVNHARRLAEDDWT 71 ++SQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVNHARRLAEDDWT Sbjct: 14 LTLSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVNHARRLAEDDWT 73 Query: 72 GMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRAL 131 GMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRAL Sbjct: 74 GMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRAL 133 Query: 132 IVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCW 191 IVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCW Sbjct: 134 IVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCW 193 Query: 192 RGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFP 251 RGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFP Sbjct: 194 RGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFP 253 Query: 252 FEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIME 311 FEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIME Sbjct: 254 FEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIME 313 Query: 312 HKKSQKEGMKEKLTHKASENGHYELEHLSTPKLKGSSHSPDHPGCLMEN 360 HKKSQKEGMKEKLTHKASENGHYELEHLSTPKLKGSSHSPDHPGCLMEN Sbjct: 314 HKKSQKEGMKEKLTHKASENGHYELEHLSTPKLKGSSHSPDHPGCLMEN 362 >5DIS-C nrPDB Length = 289 Score = 604 bits (1557), Expect = e-173, Method: Compositional matrix adjust. Identities = 287/287 (100%), Positives = 287/287 (100%) Query: 1 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN 60 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN Sbjct: 3 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN 62 Query: 61 HARRLAEDDWTGMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWI 120 HARRLAEDDWTGMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWI Sbjct: 63 HARRLAEDDWTGMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWI 122 Query: 121 VVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVN 180 VVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVN Sbjct: 123 VVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVN 182 Query: 181 QTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPE 240 QTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPE Sbjct: 183 QTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPE 242 Query: 241 SLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLG 287 SLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLG Sbjct: 243 SLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLG 289 >1XK5-A nrPDB Length = 204 Score = 437 bits (1124), Expect = e-123, Method: Compositional matrix adjust. Identities = 204/204 (100%), Positives = 204/204 (100%) Query: 97 HYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLL 156 HYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLL Sbjct: 1 HYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLL 60 Query: 157 PGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEE 216 PGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEE Sbjct: 61 PGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEE 120 Query: 217 GLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGW 276 GLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGW Sbjct: 121 GLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGW 180 Query: 277 LRPYMVSDVLGVAVPAGPLTTKPD 300 LRPYMVSDVLGVAVPAGPLTTKPD Sbjct: 181 LRPYMVSDVLGVAVPAGPLTTKPD 204 >2QNA-B nrPDB Length = 66 Score = 130 bits (328), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 66/66 (100%), Positives = 66/66 (100%) Query: 1 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN 60 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN Sbjct: 1 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN 60 Query: 61 HARRLA 66 HARRLA Sbjct: 61 HARRLA 66 >4I6Z-A nrPDB Length = 202 Score = 28.9 bits (63), Expect = 3.5, Method: Compositional matrix adjust. Identities = 18/52 (34%), Positives = 31/52 (59%), Gaps = 5/52 (9%) Query: 22 AAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRL--DYVNHARR---LAED 68 +A HP + + S+++ R+R LL+L+KS+R+ D+V + LAED Sbjct: 95 SASHPEEADFLITLVSVDEGLRKRILLDLEKSQRVFFDFVREKLKDLDLAED 146 >1QVJ-A nrPDB Length = 292 Score = 28.1 bits (61), Expect = 6.7, Method: Compositional matrix adjust. Identities = 23/68 (33%), Positives = 31/68 (45%), Gaps = 11/68 (16%) Query: 274 VGWL---RPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIMEHKKSQKEGMKEKLTHKASE 330 VGWL + Y + V+V AGP P QI E S K K+ + S+ Sbjct: 27 VGWLVEWQDYKPVEYTAVSVLAGPRWADP--------QISESNFSPKFNEKDGHVERKSK 78 Query: 331 NGHYELEH 338 NG YE+E+ Sbjct: 79 NGLYEIEN 86 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3NC0-B nrPDB 746 0.0 5DIS-C nrPDB 621 e-178 1XK5-A nrPDB 466 e-131 2QNA-B nrPDB 126 1e-29 Sequences not found previously or not previously below threshold: 4I6Z-A nrPDB 32 0.61 1QVJ-A nrPDB 30 1.4 3JCR-F nrPDB 29 3.5 CONVERGED! >3NC0-B nrPDB Length = 362 Score = 746 bits (1927), Expect = 0.0, Method: Composition-based stats. Identities = 346/349 (99%), Positives = 348/349 (99%) Query: 12 FSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVNHARRLAEDDWT 71 ++SQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVNHARRLAEDDWT Sbjct: 14 LTLSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVNHARRLAEDDWT 73 Query: 72 GMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRAL 131 GMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRAL Sbjct: 74 GMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRAL 133 Query: 132 IVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCW 191 IVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCW Sbjct: 134 IVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCW 193 Query: 192 RGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFP 251 RGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFP Sbjct: 194 RGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFP 253 Query: 252 FEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIME 311 FEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIME Sbjct: 254 FEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIME 313 Query: 312 HKKSQKEGMKEKLTHKASENGHYELEHLSTPKLKGSSHSPDHPGCLMEN 360 HKKSQKEGMKEKLTHKASENGHYELEHLSTPKLKGSSHSPDHPGCLMEN Sbjct: 314 HKKSQKEGMKEKLTHKASENGHYELEHLSTPKLKGSSHSPDHPGCLMEN 362 >5DIS-C nrPDB Length = 289 Score = 621 bits (1601), Expect = e-178, Method: Composition-based stats. Identities = 287/287 (100%), Positives = 287/287 (100%) Query: 1 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN 60 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN Sbjct: 3 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN 62 Query: 61 HARRLAEDDWTGMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWI 120 HARRLAEDDWTGMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWI Sbjct: 63 HARRLAEDDWTGMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWI 122 Query: 121 VVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVN 180 VVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVN Sbjct: 123 VVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVN 182 Query: 181 QTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPE 240 QTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPE Sbjct: 183 QTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPE 242 Query: 241 SLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLG 287 SLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLG Sbjct: 243 SLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLG 289 >1XK5-A nrPDB Length = 204 Score = 466 bits (1198), Expect = e-131, Method: Composition-based stats. Identities = 204/204 (100%), Positives = 204/204 (100%) Query: 97 HYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLL 156 HYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLL Sbjct: 1 HYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLL 60 Query: 157 PGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEE 216 PGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEE Sbjct: 61 PGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEE 120 Query: 217 GLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGW 276 GLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGW Sbjct: 121 GLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGW 180 Query: 277 LRPYMVSDVLGVAVPAGPLTTKPD 300 LRPYMVSDVLGVAVPAGPLTTKPD Sbjct: 181 LRPYMVSDVLGVAVPAGPLTTKPD 204 >2QNA-B nrPDB Length = 66 Score = 126 bits (317), Expect = 1e-29, Method: Composition-based stats. Identities = 66/66 (100%), Positives = 66/66 (100%) Query: 1 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN 60 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN Sbjct: 1 MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVN 60 Query: 61 HARRLA 66 HARRLA Sbjct: 61 HARRLA 66 >4I6Z-A nrPDB Length = 202 Score = 31.5 bits (70), Expect = 0.61, Method: Composition-based stats. Identities = 18/52 (34%), Positives = 31/52 (59%), Gaps = 5/52 (9%) Query: 22 AAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRL--DYVNHARR---LAED 68 +A HP + + S+++ R+R LL+L+KS+R+ D+V + LAED Sbjct: 95 SASHPEEADFLITLVSVDEGLRKRILLDLEKSQRVFFDFVREKLKDLDLAED 146 >1QVJ-A nrPDB Length = 292 Score = 30.4 bits (67), Expect = 1.4, Method: Composition-based stats. Identities = 23/68 (33%), Positives = 31/68 (45%), Gaps = 11/68 (16%) Query: 274 VGWL---RPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIMEHKKSQKEGMKEKLTHKASE 330 VGWL + Y + V+V AGP P QI E S K K+ + S+ Sbjct: 27 VGWLVEWQDYKPVEYTAVSVLAGPRWADP--------QISESNFSPKFNEKDGHVERKSK 78 Query: 331 NGHYELEH 338 NG YE+E+ Sbjct: 79 NGLYEIEN 86 >3JCR-F nrPDB Length = 820 Score = 28.8 bits (63), Expect = 3.5, Method: Composition-based stats. Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 15/64 (23%) Query: 39 EQSERRRRLLELQKSKRLDYVNHARRLAED---------------DWTGMESEEENKKDD 83 ++ E R+R+LE ++ KR + + R++ ED + G E EE +K Sbjct: 179 QEVEERQRMLEEERKKRKQFQDLGRKMLEDPQERERRERRERMERETNGNEDEEGRQKIR 238 Query: 84 EEMD 87 EE D Sbjct: 239 EEKD 242 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.311 0.139 0.444 Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 15,417,054 Number of Sequences: 36641 Number of extensions: 653764 Number of successful extensions: 1667 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 1642 Number of HSP's gapped (non-prelim): 33 length of query: 360 length of database: 10,336,785 effective HSP length: 98 effective length of query: 262 effective length of database: 6,745,967 effective search space: 1767443354 effective search space used: 1767443354 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.3 bits) S2: 60 (27.7 bits)