BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O95059 (124 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6AHU-H nrPDB 254 9e-69 3WZ0-C nrPDB 29 0.48 2AV5-A nrPDB 28 1.0 5K13-A nrPDB 26 5.4 3A9E-B nrPDB 25 6.1 >6AHU-H nrPDB Length = 124 Score = 254 bits (648), Expect = 9e-69, Method: Compositional matrix adjust. Identities = 124/124 (100%), Positives = 124/124 (100%) Query: 1 MPAPAATYERVVYKNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDLFGEVDAALP 60 MPAPAATYERVVYKNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDLFGEVDAALP Sbjct: 1 MPAPAATYERVVYKNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDLFGEVDAALP 60 Query: 61 LDILTYEEKTLSAILRICSSGLVKLWSSLTLLGSYKGKKCAFRVIQVSPFLLALSGNSRE 120 LDILTYEEKTLSAILRICSSGLVKLWSSLTLLGSYKGKKCAFRVIQVSPFLLALSGNSRE Sbjct: 61 LDILTYEEKTLSAILRICSSGLVKLWSSLTLLGSYKGKKCAFRVIQVSPFLLALSGNSRE 120 Query: 121 LVLD 124 LVLD Sbjct: 121 LVLD 124 >3WZ0-C nrPDB Length = 120 Score = 29.3 bits (64), Expect = 0.48, Method: Compositional matrix adjust. Identities = 14/46 (30%), Positives = 26/46 (56%) Query: 63 ILTYEEKTLSAILRICSSGLVKLWSSLTLLGSYKGKKCAFRVIQVS 108 + ++EK+ + I+R+ + +L +LT+L G K FR + VS Sbjct: 58 FIKFDEKSQTGIVRVDRKHVEELRFALTMLTEINGSKVIFRTLGVS 103 >2AV5-A nrPDB Length = 120 Score = 28.1 bits (61), Expect = 1.0, Method: Compositional matrix adjust. Identities = 24/96 (25%), Positives = 43/96 (44%), Gaps = 6/96 (6%) Query: 14 KNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDLFGEVDAALPLD-ILTYEEKTLS 72 ++ Y KV E Q N + K+ + +A GE+ A ++ ++E T + Sbjct: 13 RDKKRYIAFKVISENQ-----FNKDEIKEAIWNACLRTLGELGTAKAKPWLIKFDETTQT 67 Query: 73 AILRICSSGLVKLWSSLTLLGSYKGKKCAFRVIQVS 108 I+R + + + SLTL+ G K +V+ VS Sbjct: 68 GIIRSDRNHVYDVIFSLTLVSDINGNKAIIKVLGVS 103 >5K13-A nrPDB Length = 246 Score = 25.8 bits (55), Expect = 5.4, Method: Compositional matrix adjust. Identities = 22/75 (29%), Positives = 34/75 (45%), Gaps = 2/75 (2%) Query: 5 AATYERVVYKNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDL-FGEVDAALPLDI 63 AA + ++ + + Y + + F D G+ LN Q + DL F + LPL++ Sbjct: 83 AACLDILILRICTRYTPEQDTMTFSD-GLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEM 141 Query: 64 LTYEEKTLSAILRIC 78 E LSAI IC Sbjct: 142 DDAETGLLSAICLIC 156 >3A9E-B nrPDB Length = 269 Score = 25.4 bits (54), Expect = 6.1, Method: Compositional matrix adjust. Identities = 22/75 (29%), Positives = 34/75 (45%), Gaps = 2/75 (2%) Query: 5 AATYERVVYKNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDL-FGEVDAALPLDI 63 AA + ++ + + Y + + F D G+ LN Q + DL F + LPL++ Sbjct: 111 AACLDILILRICTRYTPEQDTMTFSD-GLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEM 169 Query: 64 LTYEEKTLSAILRIC 78 E LSAI IC Sbjct: 170 DDAETGLLSAICLIC 184 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6AHU-H nrPDB 258 5e-70 Sequences not found previously or not previously below threshold: 3WZ0-C nrPDB 29 0.58 2AV5-A nrPDB 27 2.9 5K13-A nrPDB 26 3.9 3A9E-B nrPDB 26 3.9 2WYS-A nrPDB 25 7.8 CONVERGED! >6AHU-H nrPDB Length = 124 Score = 258 bits (659), Expect = 5e-70, Method: Composition-based stats. Identities = 124/124 (100%), Positives = 124/124 (100%) Query: 1 MPAPAATYERVVYKNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDLFGEVDAALP 60 MPAPAATYERVVYKNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDLFGEVDAALP Sbjct: 1 MPAPAATYERVVYKNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDLFGEVDAALP 60 Query: 61 LDILTYEEKTLSAILRICSSGLVKLWSSLTLLGSYKGKKCAFRVIQVSPFLLALSGNSRE 120 LDILTYEEKTLSAILRICSSGLVKLWSSLTLLGSYKGKKCAFRVIQVSPFLLALSGNSRE Sbjct: 61 LDILTYEEKTLSAILRICSSGLVKLWSSLTLLGSYKGKKCAFRVIQVSPFLLALSGNSRE 120 Query: 121 LVLD 124 LVLD Sbjct: 121 LVLD 124 >3WZ0-C nrPDB Length = 120 Score = 28.8 bits (63), Expect = 0.58, Method: Composition-based stats. Identities = 14/45 (31%), Positives = 26/45 (57%) Query: 64 LTYEEKTLSAILRICSSGLVKLWSSLTLLGSYKGKKCAFRVIQVS 108 + ++EK+ + I+R+ + +L +LT+L G K FR + VS Sbjct: 59 IKFDEKSQTGIVRVDRKHVEELRFALTMLTEINGSKVIFRTLGVS 103 >2AV5-A nrPDB Length = 120 Score = 26.5 bits (57), Expect = 2.9, Method: Composition-based stats. Identities = 24/96 (25%), Positives = 43/96 (44%), Gaps = 6/96 (6%) Query: 14 KNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDLFGEVDAALPLD-ILTYEEKTLS 72 ++ Y KV E Q N + K+ + +A GE+ A ++ ++E T + Sbjct: 13 RDKKRYIAFKVISENQ-----FNKDEIKEAIWNACLRTLGELGTAKAKPWLIKFDETTQT 67 Query: 73 AILRICSSGLVKLWSSLTLLGSYKGKKCAFRVIQVS 108 I+R + + + SLTL+ G K +V+ VS Sbjct: 68 GIIRSDRNHVYDVIFSLTLVSDINGNKAIIKVLGVS 103 >5K13-A nrPDB Length = 246 Score = 26.1 bits (56), Expect = 3.9, Method: Composition-based stats. Identities = 22/75 (29%), Positives = 34/75 (45%), Gaps = 2/75 (2%) Query: 5 AATYERVVYKNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDL-FGEVDAALPLDI 63 AA + ++ + + Y + + F D G+ LN Q + DL F + LPL++ Sbjct: 83 AACLDILILRICTRYTPEQDTMTFSD-GLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEM 141 Query: 64 LTYEEKTLSAILRIC 78 E LSAI IC Sbjct: 142 DDAETGLLSAICLIC 156 >3A9E-B nrPDB Length = 269 Score = 26.1 bits (56), Expect = 3.9, Method: Composition-based stats. Identities = 22/75 (29%), Positives = 34/75 (45%), Gaps = 2/75 (2%) Query: 5 AATYERVVYKNPSEYHYMKVCLEFQDCGVGLNAAQFKQLLISAVKDL-FGEVDAALPLDI 63 AA + ++ + + Y + + F D G+ LN Q + DL F + LPL++ Sbjct: 111 AACLDILILRICTRYTPEQDTMTFSD-GLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEM 169 Query: 64 LTYEEKTLSAILRIC 78 E LSAI IC Sbjct: 170 DDAETGLLSAICLIC 184 >2WYS-A nrPDB Length = 540 Score = 25.0 bits (53), Expect = 7.8, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Query: 28 FQDCGVGLNAAQFKQLLISAVKDLFGEVDAALP-LDILTYE 67 FQD G ++ + Q L S +K++F E+ P L++ Y+ Sbjct: 282 FQDNGNWVSQSVMDQRLESYIKNMFAEIQRQYPSLNLYAYD 322 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.322 0.137 0.401 Lambda K H 0.267 0.0450 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,463,877 Number of Sequences: 36641 Number of extensions: 204317 Number of successful extensions: 348 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 340 Number of HSP's gapped (non-prelim): 16 length of query: 124 length of database: 10,336,785 effective HSP length: 85 effective length of query: 39 effective length of database: 7,222,300 effective search space: 281669700 effective search space used: 281669700 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 53 (24.9 bits)