BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O94646 (200 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5U0S-H nrPDB 407 e-114 5OQM-b nrPDB 77 6e-15 4GWP-C nrPDB 75 2e-14 5EQV-A nrPDB 30 1.00 >5U0S-H nrPDB Length = 200 Score = 407 bits (1047), Expect = e-114, Method: Compositional matrix adjust. Identities = 200/200 (100%), Positives = 200/200 (100%) Query: 1 MEDISTEKTVESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSL 60 MEDISTEKTVESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSL Sbjct: 1 MEDISTEKTVESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSL 60 Query: 61 SNNLAAHSHTLQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISS 120 SNNLAAHSHTLQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISS Sbjct: 61 SNNLAAHSHTLQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISS 120 Query: 121 QPKKKEANDAYQKDQLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATE 180 QPKKKEANDAYQKDQLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATE Sbjct: 121 QPKKKEANDAYQKDQLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATE 180 Query: 181 EQNANQMLTDILSFMKSGKR 200 EQNANQMLTDILSFMKSGKR Sbjct: 181 EQNANQMLTDILSFMKSGKR 200 >5OQM-b nrPDB Length = 223 Score = 77.0 bits (188), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 58/191 (30%), Positives = 100/191 (52%), Gaps = 12/191 (6%) Query: 11 ESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSLSNNLAAHSHT 70 ++L+A+R R+AQ+ SL + ++E L W T+ N+ LSQ+ S+++ L T Sbjct: 31 QALDAVRMRLAQLTHSLRRIRDEMSKAE-LPQWYTLQSQLNVTLSQLVSVTSTLQHFQET 89 Query: 71 LQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISSQPKKKEANDA 130 L +T +YP +FP E L+TTLLR K +PEV+EW ++E ++ K +E Sbjct: 90 LDSTVVYPLPKFPTTSHESLVTTLLRKKNIPEVDEW-MKYVRETSGVTTALLKDEEIEKL 148 Query: 131 YQKD-QLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATEEQNANQMLT 189 Q+D ++ + AR F R Y DF + +ES E + + + ++ N + Sbjct: 149 LQQDREITNWARTTF---RNEYGKHDF--KNEESLSEEHASLLVRDSKPSKPFNVD---- 199 Query: 190 DILSFMKSGKR 200 D+L F +G++ Sbjct: 200 DVLKFTFTGEK 210 >4GWP-C nrPDB Length = 407 Score = 75.1 bits (183), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 50/147 (34%), Positives = 80/147 (54%), Gaps = 6/147 (4%) Query: 11 ESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSLSNNLAAHSHT 70 ++L+A+R R+AQ+ SL + ++E L W T+ N+ LSQ+ S+++ L T Sbjct: 31 QALDAVRMRLAQLTHSLRRIRDEMSKAE-LPQWYTLQSQLNVTLSQLVSVTSTLQHFQET 89 Query: 71 LQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISSQPKKKEANDA 130 L +T +YP +FP E L+TTLLR K +PEV+EW ++E ++ K +E Sbjct: 90 LDSTVVYPLPKFPTTSHESLVTTLLRKKNIPEVDEW-MKYVRETSGVTTALLKDEEIEKL 148 Query: 131 YQKD-QLWDQARIIFMEERENYSWFDF 156 Q+D ++ + AR F R Y DF Sbjct: 149 LQQDREITNWARTTF---RNEYGKHDF 172 >5EQV-A nrPDB Length = 340 Score = 29.6 bits (65), Expect = 1.00, Method: Compositional matrix adjust. Identities = 16/58 (27%), Positives = 27/58 (46%), Gaps = 5/58 (8%) Query: 25 QSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSLSNNLAAHSHTLQTTSIYPSLEF 82 Q +AI H S P+ T+ +N LSQ+ + + H+H ++PS +F Sbjct: 201 QGADLVVAIPHSGLSSDPYKTMAENSVYYLSQVPGIDAIMFGHAH-----GVFPSKDF 253 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5U0S-H nrPDB 277 3e-75 4GWP-C nrPDB 251 2e-67 5OQM-b nrPDB 248 1e-66 Sequences not found previously or not previously below threshold: 6E0K-A nrPDB 34 0.041 2IAI-A nrPDB 33 0.086 4WBY-A nrPDB 31 0.48 5B68-A nrPDB 29 1.9 2IOQ-A nrPDB 28 2.7 1Y4U-A nrPDB 27 6.5 2GQ0-A nrPDB 27 7.8 CONVERGED! >5U0S-H nrPDB Length = 200 Score = 277 bits (707), Expect = 3e-75, Method: Composition-based stats. Identities = 200/200 (100%), Positives = 200/200 (100%) Query: 1 MEDISTEKTVESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSL 60 MEDISTEKTVESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSL Sbjct: 1 MEDISTEKTVESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSL 60 Query: 61 SNNLAAHSHTLQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISS 120 SNNLAAHSHTLQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISS Sbjct: 61 SNNLAAHSHTLQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISS 120 Query: 121 QPKKKEANDAYQKDQLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATE 180 QPKKKEANDAYQKDQLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATE Sbjct: 121 QPKKKEANDAYQKDQLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATE 180 Query: 181 EQNANQMLTDILSFMKSGKR 200 EQNANQMLTDILSFMKSGKR Sbjct: 181 EQNANQMLTDILSFMKSGKR 200 >4GWP-C nrPDB Length = 407 Score = 251 bits (640), Expect = 2e-67, Method: Composition-based stats. Identities = 58/191 (30%), Positives = 100/191 (52%), Gaps = 12/191 (6%) Query: 11 ESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSLSNNLAAHSHT 70 ++L+A+R R+AQ+ SL + ++E L W T+ N+ LSQ+ S+++ L T Sbjct: 31 QALDAVRMRLAQLTHSLRRIRDEMSKAE-LPQWYTLQSQLNVTLSQLVSVTSTLQHFQET 89 Query: 71 LQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISSQPKKKEANDA 130 L +T +YP +FP E L+TTLLR K +PEV+EW ++E ++ K +E Sbjct: 90 LDSTVVYPLPKFPTTSHESLVTTLLRKKNIPEVDEW-MKYVRETSGVTTALLKDEEIEKL 148 Query: 131 YQKD-QLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATEEQNANQMLT 189 Q+D ++ + AR F R Y DF + +ES E + + + ++ N + Sbjct: 149 LQQDREITNWARTTF---RNEYGKHDF--KNEESLSEEHASLLVRDSKPSKPFNVD---- 199 Query: 190 DILSFMKSGKR 200 D+L F +G++ Sbjct: 200 DVLKFTFTGEK 210 >5OQM-b nrPDB Length = 223 Score = 248 bits (633), Expect = 1e-66, Method: Composition-based stats. Identities = 58/191 (30%), Positives = 100/191 (52%), Gaps = 12/191 (6%) Query: 11 ESLEAIRHRIAQIVQSLTHFLAILHQSESLSPWPTIHKNFNILLSQIHSLSNNLAAHSHT 70 ++L+A+R R+AQ+ SL + ++E L W T+ N+ LSQ+ S+++ L T Sbjct: 31 QALDAVRMRLAQLTHSLRRIRDEMSKAE-LPQWYTLQSQLNVTLSQLVSVTSTLQHFQET 89 Query: 71 LQTTSIYPSLEFPVKEQEPLLTTLLRTKALPEVEEWEANTLQEYEASISSQPKKKEANDA 130 L +T +YP +FP E L+TTLLR K +PEV+EW ++E ++ K +E Sbjct: 90 LDSTVVYPLPKFPTTSHESLVTTLLRKKNIPEVDEW-MKYVRETSGVTTALLKDEEIEKL 148 Query: 131 YQKD-QLWDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATEEQNANQMLT 189 Q+D ++ + AR F R Y DF + +ES E + + + ++ N + Sbjct: 149 LQQDREITNWARTTF---RNEYGKHDF--KNEESLSEEHASLLVRDSKPSKPFNVD---- 199 Query: 190 DILSFMKSGKR 200 D+L F +G++ Sbjct: 200 DVLKFTFTGEK 210 >6E0K-A nrPDB Length = 296 Score = 34.0 bits (76), Expect = 0.041, Method: Composition-based stats. Identities = 20/116 (17%), Positives = 43/116 (37%), Gaps = 14/116 (12%) Query: 85 KEQEPLLTTLLR---TKALPEVEEWEANTLQEYEASISSQPKKKEANDAYQ-----KDQL 136 K E + L R K PEV LQ + ++ + + Q + L Sbjct: 22 KTHESIRAALDRYKWPKGKPEV------YLQGSYKNSTNIRGDSDVDVVVQLNSVFMNNL 75 Query: 137 WDQARIIFMEERENYSWFDFVTRRQESEGEFVSQRQLEIDRATEEQNANQMLTDIL 192 + + F + +Y+W DF + + + ++ ++ R T + + D++ Sbjct: 76 TAEQKRRFGFVKSDYTWNDFYSDVERALTDYYGASKVRRGRKTLKVETTYLPADVV 131 >2IAI-A nrPDB Length = 230 Score = 32.9 bits (73), Expect = 0.086, Method: Composition-based stats. Identities = 14/67 (20%), Positives = 28/67 (41%), Gaps = 1/67 (1%) Query: 89 PLLTTLLRTKALPEVEEWEANTLQEYEASISSQPKKKEANDAYQKD-QLWDQARIIFMEE 147 P +T LLR + E W +E++ +++ K A + D ++ R++F Sbjct: 123 PYVTLLLRVRGNTGTERWALERRREFDHRVAALLKDAAAEGDVRADVEVRLATRLVFGMI 182 Query: 148 RENYSWF 154 W+ Sbjct: 183 NSIVEWY 189 >4WBY-A nrPDB Length = 410 Score = 30.6 bits (67), Expect = 0.48, Method: Composition-based stats. Identities = 15/59 (25%), Positives = 21/59 (35%) Query: 95 LRTKALPEVEEWEANTLQEYEASISSQPKKKEANDAYQKDQLWDQARIIFMEERENYSW 153 LR E+ E E Q +K Y ++ D + F EER +Y W Sbjct: 214 LREDRFLEILELYRKYRVLEEIIEGFQWNEKVLQKLYALRKVVDWHALEFSEERIDYGW 272 >5B68-A nrPDB Length = 714 Score = 28.6 bits (62), Expect = 1.9, Method: Composition-based stats. Identities = 22/92 (23%), Positives = 37/92 (40%), Gaps = 5/92 (5%) Query: 78 PSLEFPVKEQE--PLLTTLLRTKALPEVE---EWEANTLQEYEASISSQPKKKEANDAYQ 132 PS P +++E PL T + T LP E E L+E +++ P + A D Sbjct: 538 PSQPGPRRQEEYRPLALTTVTTHDLPPTAGYLEGEHIALRERLGVLNTDPAAELAEDLQW 597 Query: 133 KDQLWDQARIIFMEERENYSWFDFVTRRQESE 164 + ++ D A Y + R + +E Sbjct: 598 QAEILDVAASANALPAREYVGLERDQRGELAE 629 >2IOQ-A nrPDB Length = 624 Score = 28.2 bits (61), Expect = 2.7, Method: Composition-based stats. Identities = 24/120 (20%), Positives = 45/120 (37%), Gaps = 21/120 (17%) Query: 94 LLRTKALP------EVEEWEANTLQEYEAS--ISSQPKKKEANDAYQKDQLWDQARIIFM 145 LLR K + ++EW N L E++ S + D++ + A+ Sbjct: 449 LLRKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKL--ADEVDESAK---- 502 Query: 146 EERENYSWFDFVTRRQESEGEFVS----QRQLEIDRATEEQNANQMLTDILS-FMKSGKR 200 + F+ R + GE V +L A +A++M T + F +G++ Sbjct: 503 --EAEKALTPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQK 560 >1Y4U-A nrPDB Length = 559 Score = 26.7 bits (57), Expect = 6.5, Method: Composition-based stats. Identities = 22/113 (19%), Positives = 40/113 (35%), Gaps = 20/113 (17%) Query: 94 LLRTKALP------EVEEWEANTLQEYEAS--ISSQPKKKEANDAYQKDQLWDQARIIFM 145 LLR K + ++EW N L E++ S + D++ + A+ Sbjct: 449 LLRKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKL--ADEVDESAK---- 502 Query: 146 EERENYSWFDFVTRRQESEGEFVSQR----QLEIDRATEEQNANQMLTDILSF 194 + F+ R + GE V +L A +A++M T + Sbjct: 503 --EAEKALTPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKL 553 >2GQ0-A nrPDB Length = 303 Score = 26.7 bits (57), Expect = 7.8, Method: Composition-based stats. Identities = 11/49 (22%), Positives = 17/49 (34%), Gaps = 8/49 (16%) Query: 94 LLRTKALP------EVEEWEANTLQEYEAS--ISSQPKKKEANDAYQKD 134 LLR K + ++EW N L E++ S + D Sbjct: 221 LLRKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADGD 269 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.305 0.122 0.317 Lambda K H 0.267 0.0370 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,077,804 Number of Sequences: 36641 Number of extensions: 194042 Number of successful extensions: 500 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 21 Number of HSP's that attempted gapping in prelim test: 478 Number of HSP's gapped (non-prelim): 31 length of query: 200 length of database: 10,336,785 effective HSP length: 92 effective length of query: 108 effective length of database: 6,965,813 effective search space: 752307804 effective search space used: 752307804 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.5 bits) S2: 56 (26.3 bits)