BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O94376 (138 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5U0P-U nrPDB 282 3e-77 5OQM-n nrPDB 61 1e-10 6C43-A nrPDB 31 0.20 1M1J-A nrPDB 28 1.1 3IHJ-A nrPDB 28 1.6 1O60-A nrPDB 27 2.4 5JWG-A nrPDB 27 3.7 6EK7-A nrPDB 26 4.3 6NT9-A nrPDB 25 7.8 4NLC-A nrPDB 25 7.9 3T7V-A nrPDB 25 8.3 >5U0P-U nrPDB Length = 138 Score = 282 bits (722), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 138/138 (100%), Positives = 138/138 (100%) Query: 1 MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRD 60 MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRD Sbjct: 1 MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRD 60 Query: 61 LAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQ 120 LAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQ Sbjct: 61 LAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQ 120 Query: 121 LAKRIETFGRLSCVLFQP 138 LAKRIETFGRLSCVLFQP Sbjct: 121 LAKRIETFGRLSCVLFQP 138 >5OQM-n nrPDB Length = 140 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 35/103 (33%), Positives = 57/103 (55%), Gaps = 1/103 (0%) Query: 1 MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPLPGQE-KVSDSKVNPISAEELQFAQR 59 M R TQLQ +D++ QF ++++Y+ +H F L E ++SD + EE Sbjct: 1 MTDRLTQLQICLDQMTEQFCATLNYIDKNHGFERLTVNEPQMSDKHATVVPPEEFSNTID 60 Query: 60 DLAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEE 102 +L+ D++ K QI+ LI+ LPG+ + + QL KI LQ + E Sbjct: 61 ELSTDIILKTRQINKLIDSLPGVDVSAEEQLRKIDMLQKKLVE 103 >6C43-A nrPDB Length = 477 Score = 30.8 bits (68), Expect = 0.20, Method: Composition-based stats. Identities = 15/31 (48%), Positives = 20/31 (64%) Query: 84 TAPKHQLEKIKKLQNSIEEKQLERKSLESEN 114 T PK + E + KL +SIE+ LE LES+N Sbjct: 61 TTPKARAECLLKLADSIEQNALEFARLESQN 91 >1M1J-A nrPDB Length = 491 Score = 28.1 bits (61), Expect = 1.1, Method: Compositional matrix adjust. Identities = 16/35 (45%), Positives = 25/35 (71%), Gaps = 1/35 (2%) Query: 89 QLEKIKKLQNSIEEKQLERKSLESENEDLKLQLAK 123 Q+ +IK LQNSI+E+ +E K LE + D+K++ K Sbjct: 130 QVNRIKALQNSIQEQVVEMKRLEVD-IDIKIRACK 163 >3IHJ-A nrPDB Length = 498 Score = 27.7 bits (60), Expect = 1.6, Method: Composition-based stats. Identities = 21/58 (36%), Positives = 26/58 (44%), Gaps = 6/58 (10%) Query: 34 PLPGQEKVSDSKVNPISAEELQFAQRDLAKD-----LVTKFMQIDTLINQLPGISTAP 86 P+ GQ + D VNP A E F Q K+ L K + L NQ+PGI P Sbjct: 353 PVSGQAAM-DIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNP 409 >1O60-A nrPDB Length = 292 Score = 27.3 bits (59), Expect = 2.4, Method: Compositional matrix adjust. Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 4/53 (7%) Query: 25 YLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRDLAKDLVTKFMQIDTLIN 77 +L +H P P Q K P+SA E +Q DLV F ++DT I Sbjct: 237 FLEAH----PNPNQAKCDGPSALPLSALEGFVSQMKAIDDLVKSFPELDTSIG 285 >5JWG-A nrPDB Length = 703 Score = 26.6 bits (57), Expect = 3.7, Method: Composition-based stats. Identities = 12/40 (30%), Positives = 27/40 (67%), Gaps = 1/40 (2%) Query: 56 FAQRDLAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKK 95 F LA++LV ++++ +++++ ++ A K +LE+IKK Sbjct: 86 FWSYSLAEELVNPGLEVE-IVDEITDVTAAVKKELERIKK 124 >6EK7-A nrPDB Length = 410 Score = 26.2 bits (56), Expect = 4.3, Method: Compositional matrix adjust. Identities = 18/56 (32%), Positives = 32/56 (57%), Gaps = 8/56 (14%) Query: 62 AKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDL 117 AK++++ +I LINQ+P I+ ++K L I +KQLE + ES + ++ Sbjct: 121 AKNIISTGNEIINLINQMP-ITL-------RVKTLLGDITDKQLENITYESADHEV 168 >6NT9-A nrPDB Length = 742 Score = 25.4 bits (54), Expect = 7.8, Method: Compositional matrix adjust. Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 3/63 (4%) Query: 73 DTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQLAKR---IETFG 129 +TL ++ S+ KH + I N++ E L K L+ E E + +LA+ +E FG Sbjct: 656 ETLPQKMFTASSGIKHTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFG 715 Query: 130 RLS 132 L+ Sbjct: 716 SLT 718 >4NLC-A nrPDB Length = 373 Score = 25.4 bits (54), Expect = 7.9, Method: Compositional matrix adjust. Identities = 13/39 (33%), Positives = 22/39 (56%) Query: 22 SIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRD 60 S+ + H V L + + +D +V PI AE + +AQ+D Sbjct: 184 SLAFAVDHFCQVKLNKKYQTADWRVRPIPAEMVSYAQQD 222 >3T7V-A nrPDB Length = 350 Score = 25.4 bits (54), Expect = 8.3, Method: Compositional matrix adjust. Identities = 28/107 (26%), Positives = 49/107 (45%), Gaps = 9/107 (8%) Query: 34 PLPG-QEKVSDSKVNPISAEELQFAQRDLAKDLVTKFMQIDTLI---NQLPGISTA---P 86 PL G ++K + S++ IS L F +R + L ID ++ N I T+ P Sbjct: 246 PLEGFRDKSNLSELKIISVLRLMFPKRLIPASL--DLEGIDGMVLRLNAGANIVTSILPP 303 Query: 87 KHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQLAKRIETFGRLSC 133 QLE + +EE+ + KS+ E + ++ A++ + L C Sbjct: 304 DSQLEGVANYDRDLEERDRDIKSVVRRLEIMGMKPARQADFEAVLGC 350 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5U0P-U nrPDB 216 3e-57 5OQM-n nrPDB 148 7e-37 Sequences not found previously or not previously below threshold: 6B3O-A nrPDB 30 0.49 4Z53-A nrPDB 29 0.84 4KPF-A nrPDB 28 0.99 1DEQ-A nrPDB 28 1.1 4S04-A nrPDB 27 2.1 3TEB-A nrPDB 27 2.9 4RSI-B nrPDB 26 4.9 4AN9-A nrPDB 26 5.4 4NLC-A nrPDB 25 9.8 CONVERGED! >5U0P-U nrPDB Length = 138 Score = 216 bits (549), Expect = 3e-57, Method: Composition-based stats. Identities = 138/138 (100%), Positives = 138/138 (100%) Query: 1 MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRD 60 MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRD Sbjct: 1 MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRD 60 Query: 61 LAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQ 120 LAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQ Sbjct: 61 LAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQ 120 Query: 121 LAKRIETFGRLSCVLFQP 138 LAKRIETFGRLSCVLFQP Sbjct: 121 LAKRIETFGRLSCVLFQP 138 >5OQM-n nrPDB Length = 140 Score = 148 bits (373), Expect = 7e-37, Method: Composition-based stats. Identities = 35/103 (33%), Positives = 57/103 (55%), Gaps = 1/103 (0%) Query: 1 MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPLPGQE-KVSDSKVNPISAEELQFAQR 59 M R TQLQ +D++ QF ++++Y+ +H F L E ++SD + EE Sbjct: 1 MTDRLTQLQICLDQMTEQFCATLNYIDKNHGFERLTVNEPQMSDKHATVVPPEEFSNTID 60 Query: 60 DLAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEE 102 +L+ D++ K QI+ LI+ LPG+ + + QL KI LQ + E Sbjct: 61 ELSTDIILKTRQINKLIDSLPGVDVSAEEQLRKIDMLQKKLVE 103 >6B3O-A nrPDB Length = 605 Score = 29.7 bits (65), Expect = 0.49, Method: Composition-based stats. Identities = 14/82 (17%), Positives = 43/82 (52%), Gaps = 2/82 (2%) Query: 44 SKVNPISAEELQFAQRDLAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEK 103 I+ + L L L+ +++ + + + ++ + + ++++K++++ IEE Sbjct: 513 KNQTSIAPD-LSLDFEKLNVTLLDLTYEMNRIQDAIKKLNESYINLIKRMKQIEDKIEEI 571 Query: 104 QLERKSLESENEDL-KLQLAKR 124 + ++K +E+E + K++L R Sbjct: 572 ESKQKKIENEIARIKKIKLVPR 593 >4Z53-A nrPDB Length = 742 Score = 28.9 bits (63), Expect = 0.84, Method: Composition-based stats. Identities = 17/87 (19%), Positives = 31/87 (35%), Gaps = 1/87 (1%) Query: 37 GQEKVSDSKVNPISAEELQFAQRDLAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKL 96 G +S I L + +ID L+ LPG ++ ++ Sbjct: 412 GSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAIIQGRDEI 471 Query: 97 QNSIEEKQLERKSLESENEDLKLQLAK 123 + + E + R + S+ E KL+ K Sbjct: 472 KKAYETGK-GRVVVRSKTEIEKLKGGK 497 >4KPF-A nrPDB Length = 496 Score = 28.5 bits (62), Expect = 0.99, Method: Composition-based stats. Identities = 17/87 (19%), Positives = 31/87 (35%), Gaps = 1/87 (1%) Query: 37 GQEKVSDSKVNPISAEELQFAQRDLAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKL 96 G +S I L + +ID L+ LPG ++ ++ Sbjct: 166 GSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAIIQGRDEI 225 Query: 97 QNSIEEKQLERKSLESENEDLKLQLAK 123 + + E + R + S+ E KL+ K Sbjct: 226 KKAYETGK-GRVVVRSKTEIEKLKGGK 251 >1DEQ-A nrPDB Length = 390 Score = 28.1 bits (61), Expect = 1.1, Method: Composition-based stats. Identities = 27/123 (21%), Positives = 52/123 (42%), Gaps = 11/123 (8%) Query: 3 CRCTQLQDTIDEVATQFYSSIHYL-SSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRDL 61 CR L IDEV F S I+ L S ++ + + + A + Sbjct: 52 CRMKGL---IDEVDQDFTSRINKLRDSLFNYQKNSKDSNTLTKNIVELMRGDFAKANNND 108 Query: 62 AKDLVTKFMQIDT-LINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQ 120 F QI+ L +++ + Q+++I LQ ++ ++ ++ K LE + D+K++ Sbjct: 109 NT-----FKQINEDLRSRIEILRRKVIEQVQRINLLQKNVRDQLVDMKRLEVD-IDIKIR 162 Query: 121 LAK 123 K Sbjct: 163 SCK 165 >4S04-A nrPDB Length = 232 Score = 27.3 bits (59), Expect = 2.1, Method: Composition-based stats. Identities = 20/82 (24%), Positives = 36/82 (43%), Gaps = 6/82 (7%) Query: 54 LQFAQRDLAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQ--NSIEEKQLERKSLE 111 L +R+ V DTL +++ G+ T L K L+ N+ L R + + Sbjct: 63 LSRMRREKMTQPVLILTARDTLEDRISGLDTGADDYLVKPFALEELNARIRALLRRHNNQ 122 Query: 112 SENE----DLKLQLAKRIETFG 129 +NE +L+L + +R+ G Sbjct: 123 GDNEISVGNLRLNVTRRLVWLG 144 >3TEB-A nrPDB Length = 266 Score = 27.0 bits (58), Expect = 2.9, Method: Composition-based stats. Identities = 10/29 (34%), Positives = 19/29 (65%) Query: 87 KHQLEKIKKLQNSIEEKQLERKSLESENE 115 ++Q+EKI L +I EKQ + +++ N+ Sbjct: 17 ENQMEKIDILARTIAEKQYDVIAMQEVNQ 45 >4RSI-B nrPDB Length = 397 Score = 26.2 bits (56), Expect = 4.9, Method: Composition-based stats. Identities = 14/42 (33%), Positives = 25/42 (59%) Query: 87 KHQLEKIKKLQNSIEEKQLERKSLESENEDLKLQLAKRIETF 128 + K+KK ++EEK L +K+ + E +DL L L K++ + Sbjct: 29 EETQAKLKKNVETLEEKILAKKTHKQELQDLILDLKKKLNSL 70 >4AN9-A nrPDB Length = 301 Score = 26.2 bits (56), Expect = 5.4, Method: Composition-based stats. Identities = 20/100 (20%), Positives = 39/100 (39%), Gaps = 4/100 (4%) Query: 12 IDEVATQFYSSIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRDLAKDLVTKFMQ 71 IDE+A +F + Y+S L G S + + ++ A + + F Sbjct: 159 IDEMANEFVGTRSYMSP----ERLQGTHYSVQSDIWSMGLSLVEMAVGRYPRPPMAIFEL 214 Query: 72 IDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLERKSLE 111 +D ++N+ P + LE + + + ER L+ Sbjct: 215 LDYIVNEPPPKLPSAVFSLEFQDFVNKCLIKNPAERADLK 254 >4NLC-A nrPDB Length = 373 Score = 25.0 bits (53), Expect = 9.8, Method: Composition-based stats. Identities = 13/39 (33%), Positives = 22/39 (56%) Query: 22 SIHYLSSHHDFVPLPGQEKVSDSKVNPISAEELQFAQRD 60 S+ + H V L + + +D +V PI AE + +AQ+D Sbjct: 184 SLAFAVDHFCQVKLNKKYQTADWRVRPIPAEMVSYAQQD 222 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.134 0.341 Lambda K H 0.267 0.0411 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,623,423 Number of Sequences: 36641 Number of extensions: 148820 Number of successful extensions: 689 Number of sequences better than 10.0: 31 Number of HSP's better than 10.0 without gapping: 30 Number of HSP's successfully gapped in prelim test: 39 Number of HSP's that attempted gapping in prelim test: 652 Number of HSP's gapped (non-prelim): 72 length of query: 138 length of database: 10,336,785 effective HSP length: 87 effective length of query: 51 effective length of database: 7,149,018 effective search space: 364599918 effective search space used: 364599918 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 54 (25.4 bits)