BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O88273 (168 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5HK5-E nrPDB 308 8e-85 2K8P-A nrPDB 30 0.40 5TEB-A nrPDB 29 1.3 2FFC-A nrPDB 27 5.1 2KD3-A nrPDB 27 5.9 >5HK5-E nrPDB Length = 147 Score = 308 bits (789), Expect = 8e-85, Method: Compositional matrix adjust. Identities = 147/147 (100%), Positives = 147/147 (100%) Query: 22 RKNRPAGAIPSPYKDGSSNNSERWHHQIKEVLASSQEALVVTERKYLKSDWCKTQPLRQT 81 RKNRPAGAIPSPYKDGSSNNSERWHHQIKEVLASSQEALVVTERKYLKSDWCKTQPLRQT Sbjct: 1 RKNRPAGAIPSPYKDGSSNNSERWHHQIKEVLASSQEALVVTERKYLKSDWCKTQPLRQT 60 Query: 82 VSEEGCRSRTILNRFCYGQCNSFYIPRHVKKEEDSFQSCAFCKPQRVTSVIVELECPGLD 141 VSEEGCRSRTILNRFCYGQCNSFYIPRHVKKEEDSFQSCAFCKPQRVTSVIVELECPGLD Sbjct: 61 VSEEGCRSRTILNRFCYGQCNSFYIPRHVKKEEDSFQSCAFCKPQRVTSVIVELECPGLD 120 Query: 142 PPFRIKKIQKVKHCRCMSVNLSDSDKQ 168 PPFRIKKIQKVKHCRCMSVNLSDSDKQ Sbjct: 121 PPFRIKKIQKVKHCRCMSVNLSDSDKQ 147 >2K8P-A nrPDB Length = 189 Score = 30.4 bits (67), Expect = 0.40, Method: Compositional matrix adjust. Identities = 25/93 (26%), Positives = 44/93 (47%), Gaps = 13/93 (13%) Query: 73 CKTQPLRQTVSEEGCRS-RTILNRFCYGQCN-SFYIPRHVKKEE------DSFQSCAFCK 124 C+ + V++ CRS + + C GQC + +P + + + F+ C Sbjct: 56 CRELHFTRYVTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRGKWWRPSGPDFR----CI 111 Query: 125 PQRVTSVIVELECPGLDPPFRIKKIQKVKHCRC 157 P R + V+L CPG + P R +K++ V C+C Sbjct: 112 PDRYRAQRVQLLCPGGEAP-RARKVRLVASCKC 143 >5TEB-A nrPDB Length = 168 Score = 28.9 bits (63), Expect = 1.3, Method: Compositional matrix adjust. Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 3/37 (8%) Query: 40 NNSERWHH---QIKEVLASSQEALVVTERKYLKSDWC 73 NN ER ++KE + S+ A+V+ RKY S WC Sbjct: 39 NNIERSKSIGPELKEAIKGSKIAIVLLSRKYASSSWC 75 >2FFC-A nrPDB Length = 353 Score = 26.6 bits (57), Expect = 5.1, Method: Compositional matrix adjust. Identities = 14/25 (56%), Positives = 16/25 (64%), Gaps = 1/25 (4%) Query: 35 KDGSSNNSER-WHHQIKEVLASSQE 58 K GS + R +H QIKEVLA QE Sbjct: 329 KSGSPQQAAREYHQQIKEVLAELQE 353 >2KD3-A nrPDB Length = 113 Score = 26.6 bits (57), Expect = 5.9, Method: Compositional matrix adjust. Identities = 23/84 (27%), Positives = 39/84 (46%), Gaps = 13/84 (15%) Query: 82 VSEEGCRS-RTILNRFCYGQCN-SFYIPRHVKK------EEDSFQSCAFCKPQRVTSVIV 133 +++ CRS + + C GQC + +P + + F+ C P R + V Sbjct: 33 LTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRVKWWRPNGPDFR----CIPDRYRAQRV 88 Query: 134 ELECPGLDPPFRIKKIQKVKHCRC 157 +L CPG P R +K++ V C+C Sbjct: 89 QLLCPGGAAP-RSRKVRLVASCKC 111 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5HK5-E nrPDB 307 1e-84 Sequences not found previously or not previously below threshold: 2K8P-A nrPDB 35 0.017 5TEB-A nrPDB 31 0.24 2KD3-A nrPDB 31 0.27 3M3W-A nrPDB 30 0.46 2FFC-A nrPDB 28 2.4 1ZRL-A nrPDB 28 2.5 5MYJ-B0 nrPDB 27 5.5 3JZG-A nrPDB 26 6.8 2WSI-A nrPDB 26 7.8 1M58-A nrPDB 26 8.8 CONVERGED! >5HK5-E nrPDB Length = 147 Score = 307 bits (787), Expect = 1e-84, Method: Composition-based stats. Identities = 147/147 (100%), Positives = 147/147 (100%) Query: 22 RKNRPAGAIPSPYKDGSSNNSERWHHQIKEVLASSQEALVVTERKYLKSDWCKTQPLRQT 81 RKNRPAGAIPSPYKDGSSNNSERWHHQIKEVLASSQEALVVTERKYLKSDWCKTQPLRQT Sbjct: 1 RKNRPAGAIPSPYKDGSSNNSERWHHQIKEVLASSQEALVVTERKYLKSDWCKTQPLRQT 60 Query: 82 VSEEGCRSRTILNRFCYGQCNSFYIPRHVKKEEDSFQSCAFCKPQRVTSVIVELECPGLD 141 VSEEGCRSRTILNRFCYGQCNSFYIPRHVKKEEDSFQSCAFCKPQRVTSVIVELECPGLD Sbjct: 61 VSEEGCRSRTILNRFCYGQCNSFYIPRHVKKEEDSFQSCAFCKPQRVTSVIVELECPGLD 120 Query: 142 PPFRIKKIQKVKHCRCMSVNLSDSDKQ 168 PPFRIKKIQKVKHCRCMSVNLSDSDKQ Sbjct: 121 PPFRIKKIQKVKHCRCMSVNLSDSDKQ 147 >2K8P-A nrPDB Length = 189 Score = 35.1 bits (79), Expect = 0.017, Method: Composition-based stats. Identities = 25/93 (26%), Positives = 44/93 (47%), Gaps = 13/93 (13%) Query: 73 CKTQPLRQTVSEEGCRS-RTILNRFCYGQCN-SFYIPRHVKKEE------DSFQSCAFCK 124 C+ + V++ CRS + + C GQC + +P + + + F+ C Sbjct: 56 CRELHFTRYVTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRGKWWRPSGPDFR----CI 111 Query: 125 PQRVTSVIVELECPGLDPPFRIKKIQKVKHCRC 157 P R + V+L CPG + P R +K++ V C+C Sbjct: 112 PDRYRAQRVQLLCPGGEAP-RARKVRLVASCKC 143 >5TEB-A nrPDB Length = 168 Score = 31.2 bits (69), Expect = 0.24, Method: Composition-based stats. Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 3/37 (8%) Query: 40 NNSERWHH---QIKEVLASSQEALVVTERKYLKSDWC 73 NN ER ++KE + S+ A+V+ RKY S WC Sbjct: 39 NNIERSKSIGPELKEAIKGSKIAIVLLSRKYASSSWC 75 >2KD3-A nrPDB Length = 113 Score = 30.8 bits (68), Expect = 0.27, Method: Composition-based stats. Identities = 24/93 (25%), Positives = 43/93 (46%), Gaps = 13/93 (13%) Query: 73 CKTQPLRQTVSEEGCRS-RTILNRFCYGQCN-SFYIPRHVKKEE------DSFQSCAFCK 124 C+ + +++ CRS + + C GQC + +P + + + F+ C Sbjct: 24 CRELHYTRFLTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRVKWWRPNGPDFR----CI 79 Query: 125 PQRVTSVIVELECPGLDPPFRIKKIQKVKHCRC 157 P R + V+L CPG P R +K++ V C+C Sbjct: 80 PDRYRAQRVQLLCPGGAAP-RSRKVRLVASCKC 111 >3M3W-A nrPDB Length = 320 Score = 30.1 bits (66), Expect = 0.46, Method: Composition-based stats. Identities = 28/107 (26%), Positives = 46/107 (42%), Gaps = 13/107 (12%) Query: 35 KDGSSNNSERWHHQIKEVLASSQ--------EALVVTERKYLKSDWCKTQP-LRQTVSEE 85 +DG + W ++KEV AS + E T + K+D +Q LR+ Sbjct: 133 EDGFRKAQKPWLKRLKEVEASKKSYHTARKDEKTAQTRESHAKADSSMSQEQLRKLQERV 192 Query: 86 G-CRSRTILNRFCYGQCNS---FYIPRHVKKEEDSFQSCAFCKPQRV 128 G C + Y Q + Y PR+++ E +F+SC + QR+ Sbjct: 193 GRCTKEAEKMKTQYEQTLAELNRYTPRYMEDMEQAFESCQAAERQRL 239 >2FFC-A nrPDB Length = 353 Score = 27.7 bits (60), Expect = 2.4, Method: Composition-based stats. Identities = 14/25 (56%), Positives = 16/25 (64%), Gaps = 1/25 (4%) Query: 35 KDGSSNNSER-WHHQIKEVLASSQE 58 K GS + R +H QIKEVLA QE Sbjct: 329 KSGSPQQAAREYHQQIKEVLAELQE 353 >1ZRL-A nrPDB Length = 602 Score = 27.7 bits (60), Expect = 2.5, Method: Composition-based stats. Identities = 21/98 (21%), Positives = 44/98 (44%), Gaps = 4/98 (4%) Query: 71 DWCKTQPLRQTVSEEGCRSRTILNRFCYGQCNSFYIPRHVKKEEDSFQSCAFCKPQRVTS 130 D+C+ + + C+ + + C +CNS+ KKEE + Q+ + + Q+ + Sbjct: 492 DYCQDKTKMIETLKVECKEKPCEDDNCKRKCNSYKEWISKKKEEYNKQAKQYQEYQKGNN 551 Query: 131 VIVELECPGLDPPFRIKKIQKVKHCRCMSVNLSDSDKQ 168 + E + P +KK + +C ++N D K+ Sbjct: 552 YKMYSEFKSIKPEVYLKKYSE----KCSNLNFEDEFKE 585 >5MYJ-B0 nrPDB Length = 64 Score = 26.6 bits (57), Expect = 5.5, Method: Composition-based stats. Identities = 13/46 (28%), Positives = 23/46 (50%) Query: 35 KDGSSNNSERWHHQIKEVLASSQEALVVTERKYLKSDWCKTQPLRQ 80 K SSNN +Q K V+ + + + + E LK+ W + L++ Sbjct: 11 KTVSSNNRSHAMNQTKRVVKPNLQKVTILENGELKTVWASAKALKK 56 >3JZG-A nrPDB Length = 402 Score = 26.2 bits (56), Expect = 6.8, Method: Composition-based stats. Identities = 16/52 (30%), Positives = 25/52 (48%), Gaps = 8/52 (15%) Query: 55 SSQEALVVTERKYLKSDWCKTQPLRQTVSEEGCRSRTILNRFCYGQCNSFYI 106 S + A+V + ++ D K +P V TIL RF Y QC+ +Y+ Sbjct: 284 SCENAIVCWKPGKMEDDIDKIKPSESNV--------TILGRFDYSQCDIWYM 327 >2WSI-A nrPDB Length = 306 Score = 26.2 bits (56), Expect = 7.8, Method: Composition-based stats. Identities = 15/37 (40%), Positives = 25/37 (67%), Gaps = 2/37 (5%) Query: 48 QIKEVLASSQEALVVTERKYLKSD-WCKTQPLRQTVS 83 Q +++AS+QEA+ +T RKYL S+ + + PL +S Sbjct: 22 QKSQIIASTQEAIRLT-RKYLLSEIFVRWSPLNGEIS 57 >1M58-A nrPDB Length = 106 Score = 25.8 bits (55), Expect = 8.8, Method: Composition-based stats. Identities = 12/45 (26%), Positives = 19/45 (42%) Query: 94 NRFCYGQCNSFYIPRHVKKEEDSFQSCAFCKPQRVTSVIVELECP 138 + F CN +P H K ++ S C C+ + + ECP Sbjct: 62 DEFYLSDCNRIKLPCHYKLKKSSNTICITCENKLPVHFVAVEECP 106 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.132 0.411 Lambda K H 0.267 0.0406 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,436,747 Number of Sequences: 36641 Number of extensions: 230245 Number of successful extensions: 462 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 454 Number of HSP's gapped (non-prelim): 22 length of query: 168 length of database: 10,336,785 effective HSP length: 90 effective length of query: 78 effective length of database: 7,039,095 effective search space: 549049410 effective search space used: 549049410 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 55 (25.8 bits)