BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O75629 (220 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1XHN-A nrPDB 362 e-101 4R1I-A nrPDB 30 0.98 4JF0-A nrPDB 28 2.9 6EEM-B nrPDB 27 9.2 >1XHN-A nrPDB Length = 184 Score = 362 bits (929), Expect = e-101, Method: Compositional matrix adjust. Identities = 172/172 (100%), Positives = 172/172 (100%) Query: 49 LPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDGPPGAGSGVPYFYLS 108 LPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDGPPGAGSGVPYFYLS Sbjct: 13 LPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDGPPGAGSGVPYFYLS 72 Query: 109 PLQLSVSNLQENPYATLTMTLAQTNFCKKHGFDPQSPLCVHIMLSGTVTKVNETEMDIAK 168 PLQLSVSNLQENPYATLTMTLAQTNFCKKHGFDPQSPLCVHIMLSGTVTKVNETEMDIAK Sbjct: 73 PLQLSVSNLQENPYATLTMTLAQTNFCKKHGFDPQSPLCVHIMLSGTVTKVNETEMDIAK 132 Query: 169 HSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPEEYYNVTVQ 220 HSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPEEYYNVTVQ Sbjct: 133 HSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPEEYYNVTVQ 184 >4R1I-A nrPDB Length = 522 Score = 29.6 bits (65), Expect = 0.98, Method: Compositional matrix adjust. Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 1/47 (2%) Query: 166 IAKHSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPE 212 I + + I HP+ P + NWFF + I ++ YF KIV P+ Sbjct: 199 ITQQAAQIIHPDYVVGPEA-NWFFMAASTFVIALIGYFVTEKIVEPQ 244 >4JF0-A nrPDB Length = 422 Score = 28.1 bits (61), Expect = 2.9, Method: Compositional matrix adjust. Identities = 16/59 (27%), Positives = 29/59 (49%), Gaps = 5/59 (8%) Query: 150 IMLSGTVTKVNETEMDIAKHSLFIRHPEMKT-WPSSHNWFFAKLNITNIWVLDYFGGPK 207 ++ + T+ NET +A+H +RH KT + HN F + +++ + G PK Sbjct: 117 VLFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGR----SLFTVSVGGQPK 171 >6EEM-B nrPDB Length = 512 Score = 26.6 bits (57), Expect = 9.2, Method: Compositional matrix adjust. Identities = 18/67 (26%), Positives = 27/67 (40%), Gaps = 6/67 (8%) Query: 141 DPQSPLCVHIMLSGTVTKVNETEMDIAKHSLFIRH-----PEMKTWP-SSHNWFFAKLNI 194 DP PLC L G ++ A RH + ++ ++H WFF L+ Sbjct: 270 DPIGPLCAVAKLYGIWVHIDAAYAGSACICPEFRHFIDGVEDADSFSLNAHKWFFTTLDC 329 Query: 195 TNIWVLD 201 +WV D Sbjct: 330 CCLWVKD 336 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1XHN-A nrPDB 380 e-106 Sequences not found previously or not previously below threshold: 4R1I-A nrPDB 30 0.79 4ES6-A nrPDB 28 2.6 6EEM-B nrPDB 27 5.0 5FNO-A nrPDB 27 5.3 4JF0-A nrPDB 27 7.5 CONVERGED! >1XHN-A nrPDB Length = 184 Score = 380 bits (975), Expect = e-106, Method: Composition-based stats. Identities = 172/172 (100%), Positives = 172/172 (100%) Query: 49 LPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDGPPGAGSGVPYFYLS 108 LPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDGPPGAGSGVPYFYLS Sbjct: 13 LPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDGPPGAGSGVPYFYLS 72 Query: 109 PLQLSVSNLQENPYATLTMTLAQTNFCKKHGFDPQSPLCVHIMLSGTVTKVNETEMDIAK 168 PLQLSVSNLQENPYATLTMTLAQTNFCKKHGFDPQSPLCVHIMLSGTVTKVNETEMDIAK Sbjct: 73 PLQLSVSNLQENPYATLTMTLAQTNFCKKHGFDPQSPLCVHIMLSGTVTKVNETEMDIAK 132 Query: 169 HSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPEEYYNVTVQ 220 HSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPEEYYNVTVQ Sbjct: 133 HSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPEEYYNVTVQ 184 >4R1I-A nrPDB Length = 522 Score = 30.0 bits (66), Expect = 0.79, Method: Composition-based stats. Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 1/47 (2%) Query: 166 IAKHSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPE 212 I + + I HP+ P + NWFF + I ++ YF KIV P+ Sbjct: 199 ITQQAAQIIHPDYVVGPEA-NWFFMAASTFVIALIGYFVTEKIVEPQ 244 >4ES6-A nrPDB Length = 254 Score = 28.5 bits (62), Expect = 2.6, Method: Composition-based stats. Identities = 20/55 (36%), Positives = 24/55 (43%), Gaps = 3/55 (5%) Query: 9 ARALLAALLASTLLALLVSPARGRGGRDH---GDWDEASRLPPLPPREDAARVAR 60 A LLA + A L L+VS +G DW E RLP P A +AR Sbjct: 174 AGELLARVRAERLNGLVVSSGQGLQNLYQLAAADWPEIGRLPLFVPSPRVAEMAR 228 >6EEM-B nrPDB Length = 512 Score = 27.3 bits (59), Expect = 5.0, Method: Composition-based stats. Identities = 26/102 (25%), Positives = 38/102 (37%), Gaps = 12/102 (11%) Query: 112 LSVSNLQENPYATLTMTLAQTNFCKKHG------FDPQSPLCVHIMLSGTVTKVNETEMD 165 LS ++LQ A + L C G DP PLC L G ++ Sbjct: 235 LSPNSLQSAILADIESGLVPLFLCATVGTTSSTAVDPIGPLCAVAKLYGIWVHIDAAYAG 294 Query: 166 IAKHSLFIRH-----PEMKTWP-SSHNWFFAKLNITNIWVLD 201 A RH + ++ ++H WFF L+ +WV D Sbjct: 295 SACICPEFRHFIDGVEDADSFSLNAHKWFFTTLDCCCLWVKD 336 >5FNO-A nrPDB Length = 605 Score = 27.3 bits (59), Expect = 5.3, Method: Composition-based stats. Identities = 25/93 (26%), Positives = 36/93 (38%), Gaps = 18/93 (19%) Query: 35 RDHGDWDEASRLPPLPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDG 94 RD W+ A+ + L DAA+VA ++ G L ++ + + DG Sbjct: 88 RDQTVWEGAAVVQSLRAFTDAAKVA---ANIKQNGGLNSLDDFKVL----------YQDG 134 Query: 95 -----PPGAGSGVPYFYLSPLQLSVSNLQENPY 122 P G G Y S L S+ L NPY Sbjct: 135 WKGSVPQGIARGQSENYTSDLLFSMERLSVNPY 167 >4JF0-A nrPDB Length = 422 Score = 26.9 bits (58), Expect = 7.5, Method: Composition-based stats. Identities = 16/59 (27%), Positives = 29/59 (49%), Gaps = 5/59 (8%) Query: 150 IMLSGTVTKVNETEMDIAKHSLFIRHPEMKT-WPSSHNWFFAKLNITNIWVLDYFGGPK 207 ++ + T+ NET +A+H +RH KT + HN F + +++ + G PK Sbjct: 117 VLFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGR----SLFTVSVGGQPK 171 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.135 0.418 Lambda K H 0.267 0.0414 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,418,000 Number of Sequences: 36641 Number of extensions: 378265 Number of successful extensions: 790 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 784 Number of HSP's gapped (non-prelim): 14 length of query: 220 length of database: 10,336,785 effective HSP length: 93 effective length of query: 127 effective length of database: 6,929,172 effective search space: 880004844 effective search space used: 880004844 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 57 (26.6 bits)