BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O75223 (188 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2PN7-A nrPDB 393 e-110 2QIK-A nrPDB 35 0.013 3LDU-A nrPDB 28 1.9 6G5O-A nrPDB 27 5.6 >2PN7-A nrPDB Length = 188 Score = 393 bits (1009), Expect = e-110, Method: Compositional matrix adjust. Identities = 188/188 (100%), Positives = 188/188 (100%) Query: 1 MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKT 60 MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKT Sbjct: 1 MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKT 60 Query: 61 SQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEI 120 SQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEI Sbjct: 61 SQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEI 120 Query: 121 TCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDII 180 TCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDII Sbjct: 121 TCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDII 180 Query: 181 KKGETQTL 188 KKGETQTL Sbjct: 181 KKGETQTL 188 >2QIK-A nrPDB Length = 285 Score = 35.4 bits (80), Expect = 0.013, Method: Compositional matrix adjust. Identities = 40/152 (26%), Positives = 69/152 (45%), Gaps = 22/152 (14%) Query: 19 YFAYGSNLLTERI-------HLRNPSAAFFCVARLQDFKLDFGNSQGKTSQTWHGGIATI 71 YFAYGS + R + ++P A L+ + F T + G A + Sbjct: 130 YFAYGSCMDNARFQKAGVDHYFQDPVGR----AVLKGYTTRF------TLKREDGSRADM 179 Query: 72 FQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMY--VVIEVKVATQEGKEITCRSYLMTN 129 + G GV++++ S L+ L ++EGV+S Y ++V+ + K+ C ++L+ Sbjct: 180 LED-GGTTEGVLYRIPYSALSYLYKREGVESLTYRPAFVDVEAGGRHYKD--CLTFLVLQ 236 Query: 130 YESAPPSPQYKKIICMGAKENGLPLEYQEKLK 161 E+ PQ+ +I E L E+ EKLK Sbjct: 237 KEAEIAPPQHYQIEIERGAELYLSPEFTEKLK 268 >3LDU-A nrPDB Length = 385 Score = 28.5 bits (62), Expect = 1.9, Method: Compositional matrix adjust. Identities = 11/39 (28%), Positives = 22/39 (56%), Gaps = 1/39 (2%) Query: 39 AFFCVARLQDFKLDFGNSQGKTSQTWHGGIAT-IFQSPG 76 +++ + +DF+ +FG K + ++G + T FQ PG Sbjct: 339 SYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPG 377 >6G5O-A nrPDB Length = 399 Score = 26.9 bits (58), Expect = 5.6, Method: Compositional matrix adjust. Identities = 19/70 (27%), Positives = 30/70 (42%), Gaps = 8/70 (11%) Query: 119 EITCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIED 178 E+ R +E PP + I + L EYQEKL T ++SE +E+ Sbjct: 62 EVDIRDTRQIPFEVDPPVHGVYRAIVEPWFKRPLQAEYQEKL--------TAQISEIVEE 113 Query: 179 IIKKGETQTL 188 + KG + + Sbjct: 114 TLLKGSVEVV 123 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2PN7-A nrPDB 414 e-117 Sequences not found previously or not previously below threshold: 2QIK-A nrPDB 37 0.006 3LDU-A nrPDB 29 1.7 CONVERGED! >2PN7-A nrPDB Length = 188 Score = 414 bits (1065), Expect = e-117, Method: Composition-based stats. Identities = 188/188 (100%), Positives = 188/188 (100%) Query: 1 MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKT 60 MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKT Sbjct: 1 MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKT 60 Query: 61 SQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEI 120 SQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEI Sbjct: 61 SQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEI 120 Query: 121 TCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDII 180 TCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDII Sbjct: 121 TCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDII 180 Query: 181 KKGETQTL 188 KKGETQTL Sbjct: 181 KKGETQTL 188 >2QIK-A nrPDB Length = 285 Score = 36.6 bits (83), Expect = 0.006, Method: Composition-based stats. Identities = 40/152 (26%), Positives = 69/152 (45%), Gaps = 22/152 (14%) Query: 19 YFAYGSNLLTERI-------HLRNPSAAFFCVARLQDFKLDFGNSQGKTSQTWHGGIATI 71 YFAYGS + R + ++P A L+ + F T + G A + Sbjct: 130 YFAYGSCMDNARFQKAGVDHYFQDPVGR----AVLKGYTTRF------TLKREDGSRADM 179 Query: 72 FQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMY--VVIEVKVATQEGKEITCRSYLMTN 129 + G GV++++ S L+ L ++EGV+S Y ++V+ + K+ C ++L+ Sbjct: 180 LED-GGTTEGVLYRIPYSALSYLYKREGVESLTYRPAFVDVEAGGRHYKD--CLTFLVLQ 236 Query: 130 YESAPPSPQYKKIICMGAKENGLPLEYQEKLK 161 E+ PQ+ +I E L E+ EKLK Sbjct: 237 KEAEIAPPQHYQIEIERGAELYLSPEFTEKLK 268 >3LDU-A nrPDB Length = 385 Score = 28.5 bits (62), Expect = 1.7, Method: Composition-based stats. Identities = 11/39 (28%), Positives = 22/39 (56%), Gaps = 1/39 (2%) Query: 39 AFFCVARLQDFKLDFGNSQGKTSQTWHGGIAT-IFQSPG 76 +++ + +DF+ +FG K + ++G + T FQ PG Sbjct: 339 SYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPG 377 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.132 0.390 Lambda K H 0.267 0.0411 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,891,531 Number of Sequences: 36641 Number of extensions: 314466 Number of successful extensions: 563 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 557 Number of HSP's gapped (non-prelim): 15 length of query: 188 length of database: 10,336,785 effective HSP length: 91 effective length of query: 97 effective length of database: 7,002,454 effective search space: 679238038 effective search space used: 679238038 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 56 (26.2 bits)