BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O60104 (376 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5N9J-E nrPDB 762 0.0 5SVA-U nrPDB 81 7e-16 1YKH-A nrPDB 80 1e-15 4EGS-A nrPDB 29 3.3 3FBN-A nrPDB 29 3.8 4YXM-A nrPDB 29 4.3 6CS8-A nrPDB 28 4.4 3DZV-A nrPDB 28 4.7 3FBI-A nrPDB 28 5.4 4F4H-A nrPDB 28 6.7 1JSD-B nrPDB 28 7.0 >5N9J-E nrPDB Length = 376 Score = 762 bits (1967), Expect = 0.0, Method: Compositional matrix adjust. Identities = 376/376 (100%), Positives = 376/376 (100%) Query: 1 MEPNEGVQLFSAFPPPPPYYKLFTRENIEKVISNMEKEAKHEDDANTLQPKTEEEIESLA 60 MEPNEGVQLFSAFPPPPPYYKLFTRENIEKVISNMEKEAKHEDDANTLQPKTEEEIESLA Sbjct: 1 MEPNEGVQLFSAFPPPPPYYKLFTRENIEKVISNMEKEAKHEDDANTLQPKTEEEIESLA 60 Query: 61 KLFKKPSCLTSGTYQMFGDTWRLDEAIPSLKEFGIPELYKDIKDGEDEIEVVEYDPKSNA 120 KLFKKPSCLTSGTYQMFGDTWRLDEAIPSLKEFGIPELYKDIKDGEDEIEVVEYDPKSNA Sbjct: 61 KLFKKPSCLTSGTYQMFGDTWRLDEAIPSLKEFGIPELYKDIKDGEDEIEVVEYDPKSNA 120 Query: 121 IVGTSFTRVHDYDKNSPIENEKILEDDQHTAMKEKDNESDKTMKDVEEKTEPSLKKEEED 180 IVGTSFTRVHDYDKNSPIENEKILEDDQHTAMKEKDNESDKTMKDVEEKTEPSLKKEEED Sbjct: 121 IVGTSFTRVHDYDKNSPIENEKILEDDQHTAMKEKDNESDKTMKDVEEKTEPSLKKEEED 180 Query: 181 IQMKEPLDSQDTGAVSASSVNEGFRADQKSKDGETSDLIKIPRRAYELRFLSRSLMLNFL 240 IQMKEPLDSQDTGAVSASSVNEGFRADQKSKDGETSDLIKIPRRAYELRFLSRSLMLNFL Sbjct: 181 IQMKEPLDSQDTGAVSASSVNEGFRADQKSKDGETSDLIKIPRRAYELRFLSRSLMLNFL 240 Query: 241 ELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVEL 300 ELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVEL Sbjct: 241 ELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVEL 300 Query: 301 LRTHNRQMTETLEKYKSLDFNMEKEGDVIQQLKSSIKKPLSGAEDEQKSRSMFSKNDEKL 360 LRTHNRQMTETLEKYKSLDFNMEKEGDVIQQLKSSIKKPLSGAEDEQKSRSMFSKNDEKL Sbjct: 301 LRTHNRQMTETLEKYKSLDFNMEKEGDVIQQLKSSIKKPLSGAEDEQKSRSMFSKNDEKL 360 Query: 361 KKSLELMEDVIKRDLS 376 KKSLELMEDVIKRDLS Sbjct: 361 KKSLELMEDVIKRDLS 376 >5SVA-U nrPDB Length = 222 Score = 81.3 bits (199), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 33/58 (56%), Positives = 50/58 (86%) Query: 241 ELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQV 298 EL+G+++ P+ + KVENIR +L+N+HHL+N+YRPHQSRESLIMLLE+QL+++ ++ Sbjct: 132 ELIGVLSINPDMYERKVENIRTILVNIHHLLNEYRPHQSRESLIMLLEEQLEYKRGEI 189 Score = 43.1 bits (100), Expect = 2e-04, Method: Compositional matrix adjust. Identities = 29/97 (29%), Positives = 46/97 (47%), Gaps = 4/97 (4%) Query: 20 YKLFTRENIEKVISNMEKEAKH--EDDANTLQPKTEEEIESLAKLFKKPSCLTSGTYQMF 77 K FT+ N+EK+ EK+A + N +EEEI P + Y+ F Sbjct: 20 VKFFTQSNLEKLPKYKEKKAASAKQTAPNNSNGGSEEEITCALDYLIPPPMPKNQQYRAF 79 Query: 78 GDTWRLDEAIPSLKEFGIPELYKDIKDGEDEIEVVEY 114 G W++ + +P L+ G+ +LYK K E+E +Y Sbjct: 80 GSIWQVKDQLPDLESMGLTQLYK--KSTENESTNYQY 114 >1YKH-A nrPDB Length = 108 Score = 80.5 bits (197), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 33/58 (56%), Positives = 50/58 (86%) Query: 241 ELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQV 298 EL+G+++ P+ + KVENIR +L+N+HHL+N+YRPHQSRESLIMLLE+QL+++ ++ Sbjct: 35 ELIGVLSINPDMYERKVENIRTILVNIHHLLNEYRPHQSRESLIMLLEEQLEYKRGEI 92 >4EGS-A nrPDB Length = 180 Score = 29.3 bits (64), Expect = 3.3, Method: Compositional matrix adjust. Identities = 23/84 (27%), Positives = 37/84 (44%), Gaps = 11/84 (13%) Query: 249 APEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVELLRTHNRQM 308 APE FP+ E + VL I+D+R R E+ LK + + + +H R + Sbjct: 75 APEGFPASSEAVEVLKKEYGIDISDHRAKSLR-------EEDLKGADLVLAMAFSHKRSL 127 Query: 309 TETLEKYKSLDFNMEK----EGDV 328 +Y F +++ EGDV Sbjct: 128 VSQYPEYADKIFTIKEFVGLEGDV 151 >3FBN-A nrPDB Length = 84 Score = 28.9 bits (63), Expect = 3.8, Method: Compositional matrix adjust. Identities = 19/63 (30%), Positives = 27/63 (42%), Gaps = 2/63 (3%) Query: 21 KLFTRENIEKVISNMEKEAKH--EDDANTLQPKTEEEIESLAKLFKKPSCLTSGTYQMFG 78 K FT+ N+EK+ EK+A + N +EEEI P + Y+ FG Sbjct: 22 KFFTQSNLEKLPKYKEKKAASAKQTAPNNSNGGSEEEITCALDYLIPPPMPKNQQYRAFG 81 Query: 79 DTW 81 W Sbjct: 82 SIW 84 >4YXM-A nrPDB Length = 377 Score = 28.9 bits (63), Expect = 4.3, Method: Compositional matrix adjust. Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 1/42 (2%) Query: 280 RESLIMLLEKQLKHEESQVELLRTHNRQMTETLEKYKSLDFN 321 R ++I L K K+ +QV+ L + Q TLEKYK +FN Sbjct: 149 RRNIISLYYKNYKYVVNQVDFLISKVTQAISTLEKYKD-NFN 189 >6CS8-A nrPDB Length = 303 Score = 28.5 bits (62), Expect = 4.4, Method: Compositional matrix adjust. Identities = 22/75 (29%), Positives = 32/75 (42%), Gaps = 1/75 (1%) Query: 28 IEKVISNMEKEAKHEDDANTLQPKTEEEIESLAKLFKKPSCLTSGTYQMFGDTWRLDEAI 87 I +V+ ++ EA HE T+ T + S AKLF + LT T T + Sbjct: 206 IVRVMKKLDVEAPHEV-MLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIF 264 Query: 88 PSLKEFGIPELYKDI 102 +FGIP Y + Sbjct: 265 SVADQFGIPIRYIGV 279 >3DZV-A nrPDB Length = 273 Score = 28.5 bits (62), Expect = 4.7, Method: Compositional matrix adjust. Identities = 12/26 (46%), Positives = 15/26 (57%) Query: 245 IMAKAPEQFPSKVENIRVLLLNLHHL 270 IMA P +FP + L+LNL HL Sbjct: 44 IMADDPREFPQMFQQTSALVLNLGHL 69 >3FBI-A nrPDB Length = 84 Score = 28.5 bits (62), Expect = 5.4, Method: Compositional matrix adjust. Identities = 19/64 (29%), Positives = 27/64 (42%), Gaps = 2/64 (3%) Query: 20 YKLFTRENIEKVISNMEKEAKH--EDDANTLQPKTEEEIESLAKLFKKPSCLTSGTYQMF 77 K FT+ N+EK+ EK+A + N +EEEI P + Y+ F Sbjct: 21 VKFFTQSNLEKLPKYKEKKAASAKQTAPNNSNGGSEEEITCALDYLIPPPMPKNQQYRAF 80 Query: 78 GDTW 81 G W Sbjct: 81 GSIW 84 >4F4H-A nrPDB Length = 565 Score = 28.1 bits (61), Expect = 6.7, Method: Compositional matrix adjust. Identities = 12/36 (33%), Positives = 21/36 (58%) Query: 74 YQMFGDTWRLDEAIPSLKEFGIPELYKDIKDGEDEI 109 Y M D R+D ++E G+P +Y ++ G+DE+ Sbjct: 197 YHMNKDAVRIDILRARIRETGLPMVYVNLVGGQDEL 232 >1JSD-B nrPDB Length = 176 Score = 28.1 bits (61), Expect = 7.0, Method: Compositional matrix adjust. Identities = 31/119 (26%), Positives = 59/119 (49%), Gaps = 11/119 (9%) Query: 248 KAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVELLRTHNRQ 307 KA ++ SKV NI V +N + I D+ + L M+ K + Q++ + T+N + Sbjct: 43 KAIDKITSKVNNI-VDKMNKQYGIIDHEFSEIETRLNMINNKI----DDQIQDIWTYNAE 97 Query: 308 MTETLEKYKSLDFNMEKEGDVIQQLKSSIKKPLS--GAEDEQKSRSMFSKNDEKLKKSL 364 + LE K+LD E + +V L + +K+ L ED + ++ K D++ +++ Sbjct: 98 LLVLLENQKTLD---EHDANV-NNLYNKVKRALGSNAMEDGKGCFELYHKCDDQCMETI 152 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5N9J-E nrPDB 641 0.0 5SVA-U nrPDB 144 7e-35 1YKH-A nrPDB 102 2e-22 Sequences not found previously or not previously below threshold: 3FBN-A nrPDB 104 9e-23 3FBI-A nrPDB 104 9e-23 2P14-A nrPDB 34 0.097 1D1N-A nrPDB 31 0.91 5C0S-A nrPDB 30 2.0 4F2G-A nrPDB 28 5.5 >5N9J-E nrPDB Length = 376 Score = 641 bits (1653), Expect = 0.0, Method: Composition-based stats. Identities = 376/376 (100%), Positives = 376/376 (100%) Query: 1 MEPNEGVQLFSAFPPPPPYYKLFTRENIEKVISNMEKEAKHEDDANTLQPKTEEEIESLA 60 MEPNEGVQLFSAFPPPPPYYKLFTRENIEKVISNMEKEAKHEDDANTLQPKTEEEIESLA Sbjct: 1 MEPNEGVQLFSAFPPPPPYYKLFTRENIEKVISNMEKEAKHEDDANTLQPKTEEEIESLA 60 Query: 61 KLFKKPSCLTSGTYQMFGDTWRLDEAIPSLKEFGIPELYKDIKDGEDEIEVVEYDPKSNA 120 KLFKKPSCLTSGTYQMFGDTWRLDEAIPSLKEFGIPELYKDIKDGEDEIEVVEYDPKSNA Sbjct: 61 KLFKKPSCLTSGTYQMFGDTWRLDEAIPSLKEFGIPELYKDIKDGEDEIEVVEYDPKSNA 120 Query: 121 IVGTSFTRVHDYDKNSPIENEKILEDDQHTAMKEKDNESDKTMKDVEEKTEPSLKKEEED 180 IVGTSFTRVHDYDKNSPIENEKILEDDQHTAMKEKDNESDKTMKDVEEKTEPSLKKEEED Sbjct: 121 IVGTSFTRVHDYDKNSPIENEKILEDDQHTAMKEKDNESDKTMKDVEEKTEPSLKKEEED 180 Query: 181 IQMKEPLDSQDTGAVSASSVNEGFRADQKSKDGETSDLIKIPRRAYELRFLSRSLMLNFL 240 IQMKEPLDSQDTGAVSASSVNEGFRADQKSKDGETSDLIKIPRRAYELRFLSRSLMLNFL Sbjct: 181 IQMKEPLDSQDTGAVSASSVNEGFRADQKSKDGETSDLIKIPRRAYELRFLSRSLMLNFL 240 Query: 241 ELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVEL 300 ELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVEL Sbjct: 241 ELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVEL 300 Query: 301 LRTHNRQMTETLEKYKSLDFNMEKEGDVIQQLKSSIKKPLSGAEDEQKSRSMFSKNDEKL 360 LRTHNRQMTETLEKYKSLDFNMEKEGDVIQQLKSSIKKPLSGAEDEQKSRSMFSKNDEKL Sbjct: 301 LRTHNRQMTETLEKYKSLDFNMEKEGDVIQQLKSSIKKPLSGAEDEQKSRSMFSKNDEKL 360 Query: 361 KKSLELMEDVIKRDLS 376 KKSLELMEDVIKRDLS Sbjct: 361 KKSLELMEDVIKRDLS 376 >5SVA-U nrPDB Length = 222 Score = 144 bits (363), Expect = 7e-35, Method: Composition-based stats. Identities = 29/97 (29%), Positives = 46/97 (47%), Gaps = 4/97 (4%) Query: 20 YKLFTRENIEKVISNMEKEAKH--EDDANTLQPKTEEEIESLAKLFKKPSCLTSGTYQMF 77 K FT+ N+EK+ EK+A + N +EEEI P + Y+ F Sbjct: 20 VKFFTQSNLEKLPKYKEKKAASAKQTAPNNSNGGSEEEITCALDYLIPPPMPKNQQYRAF 79 Query: 78 GDTWRLDEAIPSLKEFGIPELYKDIKDGEDEIEVVEY 114 G W++ + +P L+ G+ +LYK K E+E +Y Sbjct: 80 GSIWQVKDQLPDLESMGLTQLYK--KSTENESTNYQY 114 Score = 106 bits (264), Expect = 2e-23, Method: Composition-based stats. Identities = 35/72 (48%), Positives = 56/72 (77%) Query: 241 ELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVEL 300 EL+G+++ P+ + KVENIR +L+N+HHL+N+YRPHQSRESLIMLLE+QL+++ ++ Sbjct: 132 ELIGVLSINPDMYERKVENIRTILVNIHHLLNEYRPHQSRESLIMLLEEQLEYKRGEIRE 191 Query: 301 LRTHNRQMTETL 312 + +Q+ + L Sbjct: 192 IEQVCKQVHDKL 203 >3FBN-A nrPDB Length = 84 Score = 104 bits (258), Expect = 9e-23, Method: Composition-based stats. Identities = 19/64 (29%), Positives = 27/64 (42%), Gaps = 2/64 (3%) Query: 20 YKLFTRENIEKVISNMEKEAKH--EDDANTLQPKTEEEIESLAKLFKKPSCLTSGTYQMF 77 K FT+ N+EK+ EK+A + N +EEEI P + Y+ F Sbjct: 21 VKFFTQSNLEKLPKYKEKKAASAKQTAPNNSNGGSEEEITCALDYLIPPPMPKNQQYRAF 80 Query: 78 GDTW 81 G W Sbjct: 81 GSIW 84 >3FBI-A nrPDB Length = 84 Score = 104 bits (258), Expect = 9e-23, Method: Composition-based stats. Identities = 19/64 (29%), Positives = 27/64 (42%), Gaps = 2/64 (3%) Query: 20 YKLFTRENIEKVISNMEKEAKH--EDDANTLQPKTEEEIESLAKLFKKPSCLTSGTYQMF 77 K FT+ N+EK+ EK+A + N +EEEI P + Y+ F Sbjct: 21 VKFFTQSNLEKLPKYKEKKAASAKQTAPNNSNGGSEEEITCALDYLIPPPMPKNQQYRAF 80 Query: 78 GDTW 81 G W Sbjct: 81 GSIW 84 >1YKH-A nrPDB Length = 108 Score = 102 bits (255), Expect = 2e-22, Method: Composition-based stats. Identities = 35/72 (48%), Positives = 56/72 (77%) Query: 241 ELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVEL 300 EL+G+++ P+ + KVENIR +L+N+HHL+N+YRPHQSRESLIMLLE+QL+++ ++ Sbjct: 35 ELIGVLSINPDMYERKVENIRTILVNIHHLLNEYRPHQSRESLIMLLEEQLEYKRGEIRE 94 Query: 301 LRTHNRQMTETL 312 + +Q+ + L Sbjct: 95 IEQVCKQVHDKL 106 >2P14-A nrPDB Length = 192 Score = 34.3 bits (77), Expect = 0.097, Method: Composition-based stats. Identities = 20/73 (27%), Positives = 38/73 (52%), Gaps = 1/73 (1%) Query: 225 AYELRFLSRSLMLNFLELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLI 284 AY L FL +S+ L+F +G ++ P V+ + L+ L + P++ +E LI Sbjct: 104 AYTL-FLGKSIDLSFARHIGFNKESEPVIPLTVDQFKKLVTQLKGDGEHFNPNKLKEILI 162 Query: 285 MLLEKQLKHEESQ 297 LL L +++++ Sbjct: 163 KLLRSDLGYDQAE 175 >1D1N-A nrPDB Length = 99 Score = 30.9 bits (68), Expect = 0.91, Method: Composition-based stats. Identities = 15/56 (26%), Positives = 31/56 (55%), Gaps = 4/56 (7%) Query: 279 SRESLIMLLEKQLKHEESQVELLRTHNRQMTETLEKYKS----LDFNMEKEGDVIQ 330 +R+S + L+ + + E +++ L+ + + E + Y+ +FN KEGDVI+ Sbjct: 34 TRDSKVRLIRQGIVVYEGEIDSLKRYKDDVREVAQGYECGLTIKNFNDIKEGDVIE 89 >5C0S-A nrPDB Length = 305 Score = 29.7 bits (65), Expect = 2.0, Method: Composition-based stats. Identities = 16/63 (25%), Positives = 36/63 (57%), Gaps = 3/63 (4%) Query: 278 QSRESLIMLLEKQ---LKHEESQVELLRTHNRQMTETLEKYKSLDFNMEKEGDVIQQLKS 334 Q + +L+ +E Q L+ ++ L+T+N ++ LE ++LDF+ ++ +++KS Sbjct: 150 QQQNNLLRAIEAQQHLLQLTVWGIKQLQTYNAELLVLLENERTLDFHDSNVKNLYEKVKS 209 Query: 335 SIK 337 +K Sbjct: 210 QLK 212 >4F2G-A nrPDB Length = 309 Score = 28.2 bits (61), Expect = 5.5, Method: Composition-based stats. Identities = 18/92 (19%), Positives = 37/92 (40%), Gaps = 2/92 (2%) Query: 232 SRSLMLNFLELLGIMAKAPEQFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQL 291 S ++ ++++ I + EN RV ++N L N+Y P Q + E + Sbjct: 93 SAQVISRMVDIIMIRTFEQDIIQRFAENSRVPVIN--GLTNEYHPCQVLADIFTYYEHRG 150 Query: 292 KHEESQVELLRTHNRQMTETLEKYKSLDFNME 323 V + N + ++ + LDF ++ Sbjct: 151 PIRGKTVAWVGDANNMLYTWIQAARILDFKLQ 182 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.306 0.129 0.340 Lambda K H 0.267 0.0403 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,635,596 Number of Sequences: 36641 Number of extensions: 588233 Number of successful extensions: 1943 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 31 Number of HSP's successfully gapped in prelim test: 77 Number of HSP's that attempted gapping in prelim test: 1795 Number of HSP's gapped (non-prelim): 184 length of query: 376 length of database: 10,336,785 effective HSP length: 99 effective length of query: 277 effective length of database: 6,709,326 effective search space: 1858483302 effective search space used: 1858483302 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.5 bits) S2: 60 (27.7 bits)