BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O59543 (212 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1V77-A nrPDB 424 e-119 3WZ0-E nrPDB 242 1e-64 6AHU-I nrPDB 35 0.016 4FT6-A nrPDB 31 0.32 4CVH-A nrPDB 29 1.8 3KC2-A nrPDB 28 1.9 5TNU-A nrPDB 28 3.1 1FFT-B nrPDB 28 3.1 1R5J-A nrPDB 28 3.5 3PB0-A nrPDB 27 5.4 1CYW-A nrPDB 27 7.5 1Y7E-A nrPDB 27 7.6 1O92-A nrPDB 27 7.8 >1V77-A nrPDB Length = 212 Score = 424 bits (1089), Expect = e-119, Method: Compositional matrix adjust. Identities = 212/212 (100%), Positives = 212/212 (100%) Query: 1 MVGGGGVKFIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLS 60 MVGGGGVKFIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLS Sbjct: 1 MVGGGGVKFIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLS 60 Query: 61 NPKPSLVRDTVQKFKSYLIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLM 120 NPKPSLVRDTVQKFKSYLIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLM Sbjct: 61 NPKPSLVRDTVQKFKSYLIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLM 120 Query: 121 VKKNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRYPRD 180 VKKNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRYPRD Sbjct: 121 VKKNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRYPRD 180 Query: 181 LISLGVVIGMEIPQAKASISMYPEIILKRLKY 212 LISLGVVIGMEIPQAKASISMYPEIILKRLKY Sbjct: 181 LISLGVVIGMEIPQAKASISMYPEIILKRLKY 212 >3WZ0-E nrPDB Length = 220 Score = 242 bits (617), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 116/208 (55%), Positives = 156/208 (75%), Gaps = 6/208 (2%) Query: 9 FIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVD------KEKLREARKEYGKVAILLSNP 62 F+EMD+RD+EA+ELA +WFDEVV + K E KE+L+E R +YGKVA+LL Sbjct: 13 FVEMDVRDEEAHELASDWFDEVVFTKKLVLEDPPDWGSLKEELKELRGKYGKVALLLVTR 72 Query: 63 KPSLVRDTVQKFKSYLIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLMVK 122 KPSL+R+ + L+YV+ D+R+ R +IE GVDA+ISPW RKDPG DH LA + + Sbjct: 73 KPSLIREVKSRNLKALLYVQGGDMRINRMAIESGVDALISPWFGRKDPGFDHTLAGMAAR 132 Query: 123 KNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRYPRDLI 182 + VA+GFSL PLL +NPY RA +LRFMMK W+LV+KY+V RF+TSSA+ +W+VR PRDL+ Sbjct: 133 RGVAIGFSLSPLLNANPYGRAQILRFMMKTWQLVKKYRVPRFITSSAESRWEVRGPRDLM 192 Query: 183 SLGVVIGMEIPQAKASISMYPEIILKRL 210 SLG+ IGMEIP+A+AS++ YP I+ +L Sbjct: 193 SLGINIGMEIPEARASLNFYPRTIVWKL 220 >6AHU-I nrPDB Length = 268 Score = 35.4 bits (80), Expect = 0.016, Method: Compositional matrix adjust. Identities = 21/69 (30%), Positives = 41/69 (59%), Gaps = 2/69 (2%) Query: 134 LLYSNPYERANLLRFMMK-AWKLVEKYKVRRFLTSSAQEK-WDVRYPRDLISLGVVIGME 191 L+YS + + + R+ + A L++ K + + SSA E+ ++R P D+ +LG++ G+ Sbjct: 154 LVYSPAIKDSTMRRYTISSALNLMQICKGKNVIISSAAERPLEIRGPYDVANLGLLFGLS 213 Query: 192 IPQAKASIS 200 AKA++S Sbjct: 214 ESDAKAAVS 222 >4FT6-A nrPDB Length = 394 Score = 31.2 bits (69), Expect = 0.32, Method: Compositional matrix adjust. Identities = 16/51 (31%), Positives = 25/51 (49%), Gaps = 1/51 (1%) Query: 136 YSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRYPRDLISLGV 186 Y Y +L + LV +VR F TS+ + W +RY D ++LG+ Sbjct: 270 YQRMYGDDGMLYIAGTSTPLVNDIQVRNF-TSAGERSWQLRYDYDFVALGI 319 >4CVH-A nrPDB Length = 411 Score = 28.9 bits (63), Expect = 1.8, Method: Compositional matrix adjust. Identities = 35/144 (24%), Positives = 60/144 (41%), Gaps = 14/144 (9%) Query: 59 LSNPKPSLVRDTVQKFKSYLIYVESNDLRVIRYSIEKGVDAIISPWVNR-----KDPGID 113 LS P+ ++ D V+ F E L+V+ + E G I P V+ D +D Sbjct: 106 LSKPEVVIIHDAVRPFVE-----EGVLLKVVTAAKEHGAAGAIRPLVSTVVSPSADGCLD 160 Query: 114 HVLAKLMVKKNVALGFSLRPLLYSNPYERANL--LRFMMKAWKLVEKYKVRRF-LTSSAQ 170 + L + + + L ++Y Y++ + L F + +L KY + L + Sbjct: 161 YSLERARHRASEMPQAFLFDVIY-EAYQQCSDYDLEFGTECLQLALKYCCTKAKLVEGSP 219 Query: 171 EKWDVRYPRDLISLGVVIGMEIPQ 194 + W V Y RDL + +I I Q Sbjct: 220 DLWKVTYKRDLYAAESIIKERISQ 243 >3KC2-A nrPDB Length = 352 Score = 28.5 bits (62), Expect = 1.9, Method: Compositional matrix adjust. Identities = 26/95 (27%), Positives = 50/95 (52%), Gaps = 15/95 (15%) Query: 70 TVQKFKSYLIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLMVKKNVALGF 129 T +KF + L++ + +D I+ IIS +N ++ ++ + + K ++ + F Sbjct: 151 TTKKFDAVLVFNDPHDWAA---DIQ-----IISDAINSENGMLNTLRNEKSGKPSIPIYF 202 Query: 130 SLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRF 164 S + LL++NPY+ L RF A++L+ VRR Sbjct: 203 SNQDLLWANPYK---LNRFGQGAFRLL----VRRL 230 >5TNU-A nrPDB Length = 459 Score = 28.1 bits (61), Expect = 3.1, Method: Compositional matrix adjust. Identities = 22/77 (28%), Positives = 39/77 (50%), Gaps = 11/77 (14%) Query: 8 KFIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLSNPKPSLV 67 + +++ +DKEA E W + + +++ N + EKLRE +EY I++ Sbjct: 300 RLVKLAAKDKEAREALLAWHESLNIAV--NSQSKIEKLREILQEYKNEKIIV------FT 351 Query: 68 RDTVQKF---KSYLIYV 81 RDT + K++LI V Sbjct: 352 RDTQMAYRISKTFLIPV 368 >1FFT-B nrPDB Length = 315 Score = 28.1 bits (61), Expect = 3.1, Method: Compositional matrix adjust. Identities = 20/75 (26%), Positives = 34/75 (45%), Gaps = 4/75 (5%) Query: 3 GGGGVKFIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLSNP 62 G G+KF + D+ A++ +W + S ++ + A EY +V SN Sbjct: 214 GFSGMKFKAIATPDRAAFD---QWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVE-YFSNV 269 Query: 63 KPSLVRDTVQKFKSY 77 KP L D + KF ++ Sbjct: 270 KPDLFADVINKFMAH 284 >1R5J-A nrPDB Length = 337 Score = 27.7 bits (60), Expect = 3.5, Method: Compositional matrix adjust. Identities = 29/114 (25%), Positives = 54/114 (47%), Gaps = 12/114 (10%) Query: 78 LIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLMVKKNVALGFSLRPLLYS 137 +++ E ND RV+R + + ++ P + + + ++L K LGF+ + Sbjct: 27 IVFPEGNDERVVRAAARLKFEGLLEPIILGQSEEVRNLLTK--------LGFADQDYTII 78 Query: 138 NPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRY-PRDLISLGVVIGM 190 NP E A+ + M +A+ VE K + L + + DV Y L+ +G+ GM Sbjct: 79 NPNEYADFDK-MKEAF--VEVRKGKATLEDADKMLRDVNYFGVMLVKMGLADGM 129 >3PB0-A nrPDB Length = 300 Score = 27.3 bits (59), Expect = 5.4, Method: Compositional matrix adjust. Identities = 17/46 (36%), Positives = 27/46 (58%), Gaps = 8/46 (17%) Query: 71 VQKFKSYLIYVESNDLRVIRYSIEKGVDAII-------SPWVNRKD 109 V FK+ + +ES + R++RY +E GV+A+I SP VN + Sbjct: 16 VTPFKNGELDLESYE-RLVRYQLENGVNALIVLGTTGESPTVNEDE 60 >1CYW-A nrPDB Length = 205 Score = 26.6 bits (57), Expect = 7.5, Method: Compositional matrix adjust. Identities = 20/75 (26%), Positives = 34/75 (45%), Gaps = 4/75 (5%) Query: 3 GGGGVKFIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLSNP 62 G G+KF + D+ A++ +W + S ++ + A EY +V SN Sbjct: 104 GFSGMKFKAIATPDRAAFD---QWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVE-YFSNV 159 Query: 63 KPSLVRDTVQKFKSY 77 KP L D + KF ++ Sbjct: 160 KPDLFADVINKFMAH 174 >1Y7E-A nrPDB Length = 458 Score = 26.6 bits (57), Expect = 7.6, Method: Compositional matrix adjust. Identities = 13/41 (31%), Positives = 21/41 (51%) Query: 132 RPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEK 172 P +Y N E+ +L F K + K+K R +T+ A +K Sbjct: 5 NPWIYLNEEEKNQILNFSESYKKFISKFKTEREVTAYALDK 45 >1O92-A nrPDB Length = 396 Score = 26.6 bits (57), Expect = 7.8, Method: Compositional matrix adjust. Identities = 10/22 (45%), Positives = 17/22 (77%) Query: 30 VVVSIKFNEEVDKEKLREARKE 51 +V+S++ NE++ E +REA KE Sbjct: 204 IVISVQHNEDITLEAMREALKE 225 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1V77-A nrPDB 353 2e-98 3WZ0-E nrPDB 342 5e-95 Sequences not found previously or not previously below threshold: 6AHU-I nrPDB 36 0.013 4FT6-A nrPDB 31 0.27 5TNU-A nrPDB 29 1.9 4DDO-A nrPDB 28 3.8 4L4Q-A nrPDB 27 4.9 5UJM-B nrPDB 27 5.1 2IWZ-A nrPDB 27 6.3 1Y7E-A nrPDB 26 8.7 CONVERGED! >1V77-A nrPDB Length = 212 Score = 353 bits (907), Expect = 2e-98, Method: Composition-based stats. Identities = 212/212 (100%), Positives = 212/212 (100%) Query: 1 MVGGGGVKFIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLS 60 MVGGGGVKFIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLS Sbjct: 1 MVGGGGVKFIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLS 60 Query: 61 NPKPSLVRDTVQKFKSYLIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLM 120 NPKPSLVRDTVQKFKSYLIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLM Sbjct: 61 NPKPSLVRDTVQKFKSYLIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLM 120 Query: 121 VKKNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRYPRD 180 VKKNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRYPRD Sbjct: 121 VKKNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRYPRD 180 Query: 181 LISLGVVIGMEIPQAKASISMYPEIILKRLKY 212 LISLGVVIGMEIPQAKASISMYPEIILKRLKY Sbjct: 181 LISLGVVIGMEIPQAKASISMYPEIILKRLKY 212 >3WZ0-E nrPDB Length = 220 Score = 342 bits (878), Expect = 5e-95, Method: Composition-based stats. Identities = 116/208 (55%), Positives = 156/208 (75%), Gaps = 6/208 (2%) Query: 9 FIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVD------KEKLREARKEYGKVAILLSNP 62 F+EMD+RD+EA+ELA +WFDEVV + K E KE+L+E R +YGKVA+LL Sbjct: 13 FVEMDVRDEEAHELASDWFDEVVFTKKLVLEDPPDWGSLKEELKELRGKYGKVALLLVTR 72 Query: 63 KPSLVRDTVQKFKSYLIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLMVK 122 KPSL+R+ + L+YV+ D+R+ R +IE GVDA+ISPW RKDPG DH LA + + Sbjct: 73 KPSLIREVKSRNLKALLYVQGGDMRINRMAIESGVDALISPWFGRKDPGFDHTLAGMAAR 132 Query: 123 KNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRYPRDLI 182 + VA+GFSL PLL +NPY RA +LRFMMK W+LV+KY+V RF+TSSA+ +W+VR PRDL+ Sbjct: 133 RGVAIGFSLSPLLNANPYGRAQILRFMMKTWQLVKKYRVPRFITSSAESRWEVRGPRDLM 192 Query: 183 SLGVVIGMEIPQAKASISMYPEIILKRL 210 SLG+ IGMEIP+A+AS++ YP I+ +L Sbjct: 193 SLGINIGMEIPEARASLNFYPRTIVWKL 220 >6AHU-I nrPDB Length = 268 Score = 36.1 bits (82), Expect = 0.013, Method: Composition-based stats. Identities = 20/83 (24%), Positives = 41/83 (49%), Gaps = 5/83 (6%) Query: 119 LMVKKNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEK-WDVRY 177 + + + +A P + + R + A L++ K + + SSA E+ ++R Sbjct: 144 VAIDRGLAFELVYSPAIKDSTMRRYTI----SSALNLMQICKGKNVIISSAAERPLEIRG 199 Query: 178 PRDLISLGVVIGMEIPQAKASIS 200 P D+ +LG++ G+ AKA++S Sbjct: 200 PYDVANLGLLFGLSESDAKAAVS 222 >4FT6-A nrPDB Length = 394 Score = 31.5 bits (70), Expect = 0.27, Method: Composition-based stats. Identities = 16/51 (31%), Positives = 25/51 (49%), Gaps = 1/51 (1%) Query: 136 YSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEKWDVRYPRDLISLGV 186 Y Y +L + LV +VR F TS+ + W +RY D ++LG+ Sbjct: 270 YQRMYGDDGMLYIAGTSTPLVNDIQVRNF-TSAGERSWQLRYDYDFVALGI 319 >5TNU-A nrPDB Length = 459 Score = 28.8 bits (63), Expect = 1.9, Method: Composition-based stats. Identities = 17/68 (25%), Positives = 31/68 (45%), Gaps = 2/68 (2%) Query: 8 KFIEMDIRDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLSNPKPSLV 67 + +++ +DKEA E W + + +I N + EKLRE +EY I++ + Sbjct: 300 RLVKLAAKDKEAREALLAWHESL--NIAVNSQSKIEKLREILQEYKNEKIIVFTRDTQMA 357 Query: 68 RDTVQKFK 75 + F Sbjct: 358 YRISKTFL 365 >4DDO-A nrPDB Length = 451 Score = 27.6 bits (60), Expect = 3.8, Method: Composition-based stats. Identities = 14/61 (22%), Positives = 27/61 (44%), Gaps = 7/61 (11%) Query: 83 SNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHV-LAKLMVKKNVALGFSLRPLLYSNPYE 141 + +R+IR D +++ + D V L + ++ GFS P+ S P++ Sbjct: 201 GDAMRMIRTG---EADVVLA---GGAEAAFDKVSLGGFAAARALSTGFSEEPVRASRPFD 254 Query: 142 R 142 R Sbjct: 255 R 255 >4L4Q-A nrPDB Length = 405 Score = 27.2 bits (59), Expect = 4.9, Method: Composition-based stats. Identities = 10/29 (34%), Positives = 14/29 (48%) Query: 12 MDIRDKEAYELAKEWFDEVVVSIKFNEEV 40 +D+ K AYE+A EW + K E Sbjct: 372 IDVLQKPAYEIADEWLANITKIQKMILED 400 >5UJM-B nrPDB Length = 347 Score = 27.2 bits (59), Expect = 5.1, Method: Composition-based stats. Identities = 23/111 (20%), Positives = 44/111 (39%), Gaps = 21/111 (18%) Query: 15 RDKEAYELAKEWFDEVVVSIKFNEEVDKEKLREARKEYGKVAILLSNPKPSLVRDTVQKF 74 RDK + +L +E+F+ S + +KL+ A+ + + LLS PS + Q Sbjct: 3 RDKTS-DLVEEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVSPSFSAELKQLN 61 Query: 75 KSYLIYVESNDLRVIRYSIEKGVDAIISPWVNRKDPGIDHVLAKLMVKKNV 125 + Y + + W+ + G + VL L K+++ Sbjct: 62 QQY--------------------EKLFHKWMLQLHLGFNIVLYGLGSKRDL 92 >2IWZ-A nrPDB Length = 438 Score = 26.8 bits (58), Expect = 6.3, Method: Composition-based stats. Identities = 19/86 (22%), Positives = 31/86 (36%), Gaps = 8/86 (9%) Query: 108 KDPGIDHVLAKLMVKKNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTS 167 KD G V VA+G + PL + L F K + V + V + L + Sbjct: 110 KDSGWHPQSEADQVATGVAIGMGMIPLEVVS----ETALNFQTKGYNKVSPFFVPKILVN 165 Query: 168 SAQE----KWDVRYPRDLISLGVVIG 189 A ++ ++ P +S G Sbjct: 166 MAAGQVSIRYKLKGPNHAVSTACTTG 191 >1Y7E-A nrPDB Length = 458 Score = 26.5 bits (57), Expect = 8.7, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 21/41 (51%) Query: 132 RPLLYSNPYERANLLRFMMKAWKLVEKYKVRRFLTSSAQEK 172 P +Y N E+ +L F K + K+K R +T+ A +K Sbjct: 5 NPWIYLNEEEKNQILNFSESYKKFISKFKTEREVTAYALDK 45 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.144 0.424 Lambda K H 0.267 0.0434 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,055,079 Number of Sequences: 36641 Number of extensions: 370306 Number of successful extensions: 1291 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 24 Number of HSP's that attempted gapping in prelim test: 1266 Number of HSP's gapped (non-prelim): 46 length of query: 212 length of database: 10,336,785 effective HSP length: 93 effective length of query: 119 effective length of database: 6,929,172 effective search space: 824571468 effective search space used: 824571468 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 57 (26.5 bits)