BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O59425 (127 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2ZAE-A nrPDB 252 3e-68 2KI7-A nrPDB 212 3e-56 1V76-A nrPDB 191 5e-50 1OQK-A nrPDB 80 3e-16 6AHU-D nrPDB 42 7e-05 1TSF-A nrPDB 39 4e-04 1PC0-A nrPDB 33 0.025 4V6W-CC nrPDB 28 1.4 3HRZ-A nrPDB 27 2.0 5TF2-A nrPDB 26 3.9 2VNC-A nrPDB 26 4.1 4YE5-A nrPDB 25 7.7 >2ZAE-A nrPDB Length = 127 Score = 252 bits (644), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 126/127 (99%), Positives = 126/127 (99%) Query: 1 MRRNSKERKNRATRRSQGSYQEIIGRTWIFRGAHRGRVTRRNIIWHELIGLRVRIVGSTH 60 MRRNSKERKNRATRRSQGSYQEIIGRTWIFRGAHRGRVTRRNIIWHELIGLRVRIVGSTH Sbjct: 1 MRRNSKERKNRATRRSQGSYQEIIGRTWIFRGAHRGRVTRRNIIWHELIGLRVRIVGSTH 60 Query: 61 PAFVGIEGYVIDETRNMLVIAGDRIWKVPKDVCIFEFEADDGTKIKIPGERLVGRPEMRL 120 PAFVGIEGYVIDETRNMLVIAGDRIWKVPKDV IFEFEADDGTKIKIPGERLVGRPEMRL Sbjct: 61 PAFVGIEGYVIDETRNMLVIAGDRIWKVPKDVSIFEFEADDGTKIKIPGERLVGRPEMRL 120 Query: 121 KKRWKKW 127 KKRWKKW Sbjct: 121 KKRWKKW 127 >2KI7-A nrPDB Length = 127 Score = 212 bits (540), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 103/127 (81%), Positives = 117/127 (92%), Gaps = 1/127 (0%) Query: 1 MRRNSKERKNRATRRSQGSYQEIIGRTWIFRGAHRGRVTRRNIIWHELIGLRVRIVGSTH 60 M RNS+ER+NR + RSQGSYQEIIGRTWIFRGAHRGRV ++NI+WHELIGL+VR+V STH Sbjct: 1 MWRNSEERENRTSGRSQGSYQEIIGRTWIFRGAHRGRVNKKNIVWHELIGLKVRVVNSTH 60 Query: 61 PAFVGIEGYVIDETRNMLVIAG-DRIWKVPKDVCIFEFEADDGTKIKIPGERLVGRPEMR 119 P +VGIEGYVIDETRNMLVIAG +++WKVPKDVCIFEFE DGTKIKI GE+LVGRPEMR Sbjct: 61 PGYVGIEGYVIDETRNMLVIAGENKVWKVPKDVCIFEFETWDGTKIKISGEKLVGRPEMR 120 Query: 120 LKKRWKK 126 LKKRW+K Sbjct: 121 LKKRWRK 127 >1V76-A nrPDB Length = 96 Score = 191 bits (486), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 95/96 (98%), Positives = 95/96 (98%) Query: 32 GAHRGRVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKD 91 GAHRGRVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKD Sbjct: 1 GAHRGRVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKD 60 Query: 92 VCIFEFEADDGTKIKIPGERLVGRPEMRLKKRWKKW 127 V IFEFEADDGTKIKIPGERLVGRPEMRLKKRWKKW Sbjct: 61 VSIFEFEADDGTKIKIPGERLVGRPEMRLKKRWKKW 96 >1OQK-A nrPDB Length = 97 Score = 79.7 bits (195), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 45/91 (49%), Positives = 55/91 (60%), Gaps = 2/91 (2%) Query: 38 VTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGD--RIWKVPKDVCIF 95 +T RNI HELIGL VRI S H GI G V+DETRN L I D R VPK + +F Sbjct: 6 ITPRNIFRHELIGLSVRIARSVHRDIQGISGRVVDETRNTLRIEMDDGREITVPKGIAVF 65 Query: 96 EFEADDGTKIKIPGERLVGRPEMRLKKRWKK 126 F G ++I G LV RPE R+KK+++K Sbjct: 66 HFRTPQGELVEIDGRALVARPEERIKKKFRK 96 >6AHU-D nrPDB Length = 220 Score = 42.0 bits (97), Expect = 7e-05, Method: Compositional matrix adjust. Identities = 25/91 (27%), Positives = 48/91 (52%), Gaps = 3/91 (3%) Query: 37 RVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNM--LVIAGDRIWKVPKDVCI 94 ++ + ++ +L G + + S P++VGI G ++ ET+++ ++ DR+ +PK C+ Sbjct: 124 QMIQAKLLKADLHGAIISVTKSKCPSYVGITGILLQETKHIFKIITKEDRLKVIPKLNCV 183 Query: 95 FEFEADDGTKIKIPGERLVGRPEMRLKKRWK 125 F E DG I G + R R K++K Sbjct: 184 FTVET-DGFISYIYGSKFQLRSSERSAKKFK 213 >1TSF-A nrPDB Length = 102 Score = 39.3 bits (90), Expect = 4e-04, Method: Compositional matrix adjust. Identities = 31/88 (35%), Positives = 48/88 (54%) Query: 35 RGRVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKDVCI 94 RGR+ +I + IGL V +V S + + VGI+G V+DET+N L I ++ KV Sbjct: 2 RGRLQGVELIARDWIGLMVEVVESPNHSEVGIKGEVVDETQNTLKIMTEKGLKVVAKRGR 61 Query: 95 FEFEADDGTKIKIPGERLVGRPEMRLKK 122 G ++I G+ + RPE R+K+ Sbjct: 62 TFRVWYKGKIMRIKGDLINFRPEDRIKR 89 >1PC0-A nrPDB Length = 61 Score = 33.5 bits (75), Expect = 0.025, Method: Compositional matrix adjust. Identities = 18/39 (46%), Positives = 26/39 (66%) Query: 50 GLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKV 88 GL V +V S + + VGI+G V+DET+N L I ++ KV Sbjct: 1 GLMVEVVESPNHSEVGIKGEVVDETQNTLKIMTEKGLKV 39 >4V6W-CC nrPDB Length = 401 Score = 27.7 bits (60), Expect = 1.4, Method: Compositional matrix adjust. Identities = 16/53 (30%), Positives = 23/53 (43%), Gaps = 7/53 (13%) Query: 45 WHELIGLRVR-------IVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPK 90 WH + + R I S PA V +G+VID ++ D + KV K Sbjct: 114 WHRKVNVNQRRYALVSAIAASGVPALVQSKGHVIDGVSEFPLVVSDEVQKVQK 166 >3HRZ-A nrPDB Length = 627 Score = 27.3 bits (59), Expect = 2.0, Method: Composition-based stats. Identities = 22/89 (24%), Positives = 35/89 (39%), Gaps = 10/89 (11%) Query: 36 GRVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKDVCIF 95 GR RR+ L+ + + I P+F + Y + I D +W KD C+ Sbjct: 471 GRQPRRD--GQNLVTMNLHITPDLIPSFRFVAYYQVGNNE----IVADSVWVDVKDTCMG 524 Query: 96 EFEADDGTKIKIPGE----RLVGRPEMRL 120 I++PG +L G P R+ Sbjct: 525 TLVVKGDNLIQMPGAAMKIKLEGDPGARV 553 >5TF2-A nrPDB Length = 407 Score = 26.2 bits (56), Expect = 3.9, Method: Composition-based stats. Identities = 19/64 (29%), Positives = 29/64 (45%), Gaps = 7/64 (10%) Query: 46 HELIGLRVRIVGSTHPAFVG--IEGYVIDETRNMLVIAGD-----RIWKVPKDVCIFEFE 98 HE + LRV + + F ++ V N L+ G R+WKVP + EF+ Sbjct: 152 HEGLELRVENLQAVQTDFSSDPLQKVVCFNHDNTLLATGGTDGYVRVWKVPSLEKVLEFK 211 Query: 99 ADDG 102 A +G Sbjct: 212 AHEG 215 >2VNC-A nrPDB Length = 718 Score = 26.2 bits (56), Expect = 4.1, Method: Composition-based stats. Identities = 26/109 (23%), Positives = 42/109 (38%), Gaps = 23/109 (21%) Query: 4 NSKERKNRATRRSQGSYQEIIGRTWIFRGAHRGRVTRRNIIWHELIGLRVRIVGSTHPAF 63 N ERK++ + E + + F AH R +L G+ ++ V F Sbjct: 569 NLDERKSK--------FLEFVKKMIQFYRAHPAFRRERYFQGKKLFGMPLKDV-----TF 615 Query: 64 VGIEGYVIDETRNMLVIAGDRIWKVPKDVCIFEFEADDGTKIKIPGERL 112 +EG +DE + W P + IF E +I + GER+ Sbjct: 616 YTLEGREVDE----------KTWSSPTQLVIFVLEGSVMDEINMYGERI 654 >4YE5-A nrPDB Length = 566 Score = 25.0 bits (53), Expect = 7.7, Method: Composition-based stats. Identities = 16/55 (29%), Positives = 29/55 (52%), Gaps = 5/55 (9%) Query: 57 GSTHPAFVGIEGYVIDETRNM-LVIAGDRIWKVPKDVCIFEFEADDGTKIKIPGE 110 G+ H GI ++++ N+ +VIAGD++ + I +F T + +PGE Sbjct: 329 GNEHWTLAGI----LEQSSNVGMVIAGDKMTNEQRYNFISKFGIGQATGLNLPGE 379 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2ZAE-A nrPDB 178 7e-46 2KI7-A nrPDB 174 1e-44 1V76-A nrPDB 137 2e-33 6AHU-D nrPDB 101 7e-23 1OQK-A nrPDB 101 1e-22 1TSF-A nrPDB 97 1e-21 Sequences not found previously or not previously below threshold: 1PC0-A nrPDB 69 6e-13 6AGB-D nrPDB 48 1e-06 2CPQ-A nrPDB 27 2.9 5A8W-C nrPDB 25 7.5 2NSM-A nrPDB 25 8.1 3AU7-A nrPDB 25 8.4 >2ZAE-A nrPDB Length = 127 Score = 178 bits (451), Expect = 7e-46, Method: Composition-based stats. Identities = 126/127 (99%), Positives = 126/127 (99%) Query: 1 MRRNSKERKNRATRRSQGSYQEIIGRTWIFRGAHRGRVTRRNIIWHELIGLRVRIVGSTH 60 MRRNSKERKNRATRRSQGSYQEIIGRTWIFRGAHRGRVTRRNIIWHELIGLRVRIVGSTH Sbjct: 1 MRRNSKERKNRATRRSQGSYQEIIGRTWIFRGAHRGRVTRRNIIWHELIGLRVRIVGSTH 60 Query: 61 PAFVGIEGYVIDETRNMLVIAGDRIWKVPKDVCIFEFEADDGTKIKIPGERLVGRPEMRL 120 PAFVGIEGYVIDETRNMLVIAGDRIWKVPKDV IFEFEADDGTKIKIPGERLVGRPEMRL Sbjct: 61 PAFVGIEGYVIDETRNMLVIAGDRIWKVPKDVSIFEFEADDGTKIKIPGERLVGRPEMRL 120 Query: 121 KKRWKKW 127 KKRWKKW Sbjct: 121 KKRWKKW 127 >2KI7-A nrPDB Length = 127 Score = 174 bits (440), Expect = 1e-44, Method: Composition-based stats. Identities = 103/127 (81%), Positives = 117/127 (92%), Gaps = 1/127 (0%) Query: 1 MRRNSKERKNRATRRSQGSYQEIIGRTWIFRGAHRGRVTRRNIIWHELIGLRVRIVGSTH 60 M RNS+ER+NR + RSQGSYQEIIGRTWIFRGAHRGRV ++NI+WHELIGL+VR+V STH Sbjct: 1 MWRNSEERENRTSGRSQGSYQEIIGRTWIFRGAHRGRVNKKNIVWHELIGLKVRVVNSTH 60 Query: 61 PAFVGIEGYVIDETRNMLVIAGD-RIWKVPKDVCIFEFEADDGTKIKIPGERLVGRPEMR 119 P +VGIEGYVIDETRNMLVIAG+ ++WKVPKDVCIFEFE DGTKIKI GE+LVGRPEMR Sbjct: 61 PGYVGIEGYVIDETRNMLVIAGENKVWKVPKDVCIFEFETWDGTKIKISGEKLVGRPEMR 120 Query: 120 LKKRWKK 126 LKKRW+K Sbjct: 121 LKKRWRK 127 >1V76-A nrPDB Length = 96 Score = 137 bits (344), Expect = 2e-33, Method: Composition-based stats. Identities = 95/96 (98%), Positives = 95/96 (98%) Query: 32 GAHRGRVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKD 91 GAHRGRVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKD Sbjct: 1 GAHRGRVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKD 60 Query: 92 VCIFEFEADDGTKIKIPGERLVGRPEMRLKKRWKKW 127 V IFEFEADDGTKIKIPGERLVGRPEMRLKKRWKKW Sbjct: 61 VSIFEFEADDGTKIKIPGERLVGRPEMRLKKRWKKW 96 >6AHU-D nrPDB Length = 220 Score = 101 bits (252), Expect = 7e-23, Method: Composition-based stats. Identities = 25/91 (27%), Positives = 48/91 (52%), Gaps = 3/91 (3%) Query: 37 RVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNM--LVIAGDRIWKVPKDVCI 94 ++ + ++ +L G + + S P++VGI G ++ ET+++ ++ DR+ +PK C+ Sbjct: 124 QMIQAKLLKADLHGAIISVTKSKCPSYVGITGILLQETKHIFKIITKEDRLKVIPKLNCV 183 Query: 95 FEFEADDGTKIKIPGERLVGRPEMRLKKRWK 125 F E DG I G + R R K++K Sbjct: 184 FTVET-DGFISYIYGSKFQLRSSERSAKKFK 213 >1OQK-A nrPDB Length = 97 Score = 101 bits (251), Expect = 1e-22, Method: Composition-based stats. Identities = 45/91 (49%), Positives = 55/91 (60%), Gaps = 2/91 (2%) Query: 38 VTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGD--RIWKVPKDVCIF 95 +T RNI HELIGL VRI S H GI G V+DETRN L I D R VPK + +F Sbjct: 6 ITPRNIFRHELIGLSVRIARSVHRDIQGISGRVVDETRNTLRIEMDDGREITVPKGIAVF 65 Query: 96 EFEADDGTKIKIPGERLVGRPEMRLKKRWKK 126 F G ++I G LV RPE R+KK+++K Sbjct: 66 HFRTPQGELVEIDGRALVARPEERIKKKFRK 96 >1TSF-A nrPDB Length = 102 Score = 97.4 bits (241), Expect = 1e-21, Method: Composition-based stats. Identities = 31/88 (35%), Positives = 48/88 (54%) Query: 35 RGRVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKDVCI 94 RGR+ +I + IGL V +V S + + VGI+G V+DET+N L I ++ KV Sbjct: 2 RGRLQGVELIARDWIGLMVEVVESPNHSEVGIKGEVVDETQNTLKIMTEKGLKVVAKRGR 61 Query: 95 FEFEADDGTKIKIPGERLVGRPEMRLKK 122 G ++I G+ + RPE R+K+ Sbjct: 62 TFRVWYKGKIMRIKGDLINFRPEDRIKR 89 >1PC0-A nrPDB Length = 61 Score = 68.5 bits (166), Expect = 6e-13, Method: Composition-based stats. Identities = 21/61 (34%), Positives = 32/61 (52%) Query: 50 GLRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKDVCIFEFEADDGTKIKIPG 109 GL V +V S + + VGI+G V+DET+N L I ++ KV G ++I G Sbjct: 1 GLMVEVVESPNHSEVGIKGEVVDETQNTLKIMTEKGLKVVAKRGRTFRVWYKGKIMRIKG 60 Query: 110 E 110 + Sbjct: 61 D 61 >6AGB-D nrPDB Length = 279 Score = 47.7 bits (112), Expect = 1e-06, Method: Composition-based stats. Identities = 22/101 (21%), Positives = 46/101 (45%), Gaps = 11/101 (10%) Query: 36 GRVTRRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRN--MLVIAG---DRIWKVPK 90 G + + + G +R+ S + +G++G VI +++ ++++ G D I +PK Sbjct: 166 GSLALLKLSMADYNGALLRVTKSKNKTLIGLQGIVIWDSQKFFIMIVKGNIIDEIKCIPK 225 Query: 91 DVCIFEFEAD------DGTKIKIPGERLVGRPEMRLKKRWK 125 +F+FE + I G+R R R +++K Sbjct: 226 KGTVFQFEIPISDDDDSALRYSILGDRFKYRSVDRAGRKFK 266 >2CPQ-A nrPDB Length = 91 Score = 26.5 bits (57), Expect = 2.9, Method: Composition-based stats. Identities = 15/61 (24%), Positives = 23/61 (37%), Gaps = 3/61 (4%) Query: 43 IIWHELIGLRVRIVGS---THPAFVGIEGYVIDETRNMLVIAGDRIWKVPKDVCIFEFEA 99 ++ +L+GL + GS G+ +DE I G+ V K EF Sbjct: 20 VVREDLMGLAIGTHGSNIQQARKVPGVTAIELDEDTGTFRIYGESADAVKKARGFLEFVE 79 Query: 100 D 100 D Sbjct: 80 D 80 >5A8W-C nrPDB Length = 265 Score = 25.4 bits (54), Expect = 7.5, Method: Composition-based stats. Identities = 22/94 (23%), Positives = 40/94 (42%), Gaps = 11/94 (11%) Query: 28 WIFRGAHRGRVTRRNIIWH---ELIGLRVRIVGST--HPAFVGIEGYVIDETRNMLVIAG 82 W +RG G ++ R +I +L G+ +V + PA GI G + + L + Sbjct: 110 WRYRGVDTGTLSGRQVIEMRELDLEGVSKELVETELFDPATTGIRGATVH--GHSLRLDE 167 Query: 83 DRIWKVPKDVCIFEFEADDGTKIKIPGERLVGRP 116 + + +F+ E G + + + VGRP Sbjct: 168 NGLMFDALQRYVFDEET--GHVVYVKEQ--VGRP 197 >2NSM-A nrPDB Length = 439 Score = 25.0 bits (53), Expect = 8.1, Method: Composition-based stats. Identities = 9/39 (23%), Positives = 17/39 (43%) Query: 40 RRNIIWHELIGLRVRIVGSTHPAFVGIEGYVIDETRNML 78 + E +G + ++ GI+G V+D+ N L Sbjct: 297 PEEELQREWLGNKEALIQFLEQVHQGIKGMVLDQNYNNL 335 >3AU7-A nrPDB Length = 402 Score = 25.0 bits (53), Expect = 8.4, Method: Composition-based stats. Identities = 18/75 (24%), Positives = 28/75 (37%), Gaps = 5/75 (6%) Query: 51 LRVRIVGSTHPAFVGIEGYVIDETRNMLVIAGDRIWKVPKDVCIFEFEADDGTKIKIPGE 110 + V I + P GI G ++ L A + + P D F + T + + GE Sbjct: 221 VVVCIPNTPCPVLYGIRGESVE----ALYKAMESVKTEPVDR-RMIFVTNHATDMHLIGE 275 Query: 111 RLVGRPEMRLKKRWK 125 V R E R + Sbjct: 276 EEVHRLENYRSYRLR 290 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.138 0.387 Lambda K H 0.267 0.0420 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,355,737 Number of Sequences: 36641 Number of extensions: 203437 Number of successful extensions: 653 Number of sequences better than 10.0: 26 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 22 Number of HSP's that attempted gapping in prelim test: 624 Number of HSP's gapped (non-prelim): 43 length of query: 127 length of database: 10,336,785 effective HSP length: 86 effective length of query: 41 effective length of database: 7,185,659 effective search space: 294612019 effective search space used: 294612019 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.2 bits) S2: 53 (25.0 bits)