BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O59248 (120 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1X0T-A nrPDB 239 2e-64 2KI7-B nrPDB 168 4e-43 5T2N-A nrPDB 31 0.11 6AGB-K nrPDB 28 0.84 4UQI-B nrPDB 27 1.6 5UJ7-E nrPDB 27 2.3 4KOO-A nrPDB 27 2.6 5UJM-E nrPDB 26 3.2 5OHX-A nrPDB 25 7.6 3BNI-A nrPDB 25 9.4 >1X0T-A nrPDB Length = 120 Score = 239 bits (609), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 120/120 (100%), Positives = 120/120 (100%) Query: 1 MVDIVKRRDWEKKEKKKIAIERIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIP 60 MVDIVKRRDWEKKEKKKIAIERIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIP Sbjct: 1 MVDIVKRRDWEKKEKKKIAIERIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIP 60 Query: 61 RKWKRRYCKRCHTFLIPGVNARVRLRTKRMPHVVITCLECGYIMRYPYLREVKQKRKKAT 120 RKWKRRYCKRCHTFLIPGVNARVRLRTKRMPHVVITCLECGYIMRYPYLREVKQKRKKAT Sbjct: 61 RKWKRRYCKRCHTFLIPGVNARVRLRTKRMPHVVITCLECGYIMRYPYLREVKQKRKKAT 120 >2KI7-B nrPDB Length = 123 Score = 168 bits (426), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 79/98 (80%), Positives = 90/98 (91%) Query: 11 EKKEKKKIAIERIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIPRKWKRRYCKR 70 EKKEKK+IA ERID LF+LAERV YSP+LAKRYVELAL +Q+KAKVKIPRKWKRRYCK+ Sbjct: 6 EKKEKKRIAKERIDILFSLAERVFPYSPELAKRYVELALLVQQKAKVKIPRKWKRRYCKK 65 Query: 71 CHTFLIPGVNARVRLRTKRMPHVVITCLECGYIMRYPY 108 CH FL+PG+NARVRLR KRMPH+V+ CLECG+IMRYPY Sbjct: 66 CHAFLVPGINARVRLRQKRMPHIVVKCLECGHIMRYPY 103 >5T2N-A nrPDB Length = 296 Score = 31.2 bits (69), Expect = 0.11, Method: Compositional matrix adjust. Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 2/43 (4%) Query: 63 WKRRYCKRCHTFLIPGVNARVRLRTKRMPHVVITCLECGYIMR 105 WKR+ L+ G++ +R K + + +IT L CGYI+ Sbjct: 181 WKRKGGTHVGVQLVFGISQHIR--DKNLMNSLITYLGCGYILE 221 >6AGB-K nrPDB Length = 144 Score = 28.1 bits (61), Expect = 0.84, Method: Compositional matrix adjust. Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 1/44 (2%) Query: 32 RVARYSPDLAKRYVELALEIQKKAKVKIPRKWKRRYCKRCHTFL 75 R ++P LA+ Y++ I KK K + KR CK+CH L Sbjct: 55 RTDAHTP-LARNYIKSMDLISKKTKTSLLPTIKRTICKKCHRLL 97 >4UQI-B nrPDB Length = 657 Score = 27.3 bits (59), Expect = 1.6, Method: Composition-based stats. Identities = 14/43 (32%), Positives = 25/43 (58%) Query: 11 EKKEKKKIAIERIDTLFTLAERVARYSPDLAKRYVELALEIQK 53 EKKEK+K A++++ T+ + V+ PD+ LE++K Sbjct: 25 EKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKK 67 >5UJ7-E nrPDB Length = 284 Score = 26.6 bits (57), Expect = 2.3, Method: Compositional matrix adjust. Identities = 8/20 (40%), Positives = 13/20 (65%) Query: 81 ARVRLRTKRMPHVVITCLEC 100 + L+T +PHV + C+EC Sbjct: 47 TQTLLKTLELPHVFVNCVEC 66 >4KOO-A nrPDB Length = 180 Score = 26.6 bits (57), Expect = 2.6, Method: Compositional matrix adjust. Identities = 10/28 (35%), Positives = 17/28 (60%) Query: 5 VKRRDWEKKEKKKIAIERIDTLFTLAER 32 V++ DW KK+ +++ I TL +L R Sbjct: 57 VRQYDWSKKQVFSLSVTEIGTLVSLGPR 84 >5UJM-E nrPDB Length = 435 Score = 26.2 bits (56), Expect = 3.2, Method: Composition-based stats. Identities = 8/16 (50%), Positives = 12/16 (75%) Query: 85 LRTKRMPHVVITCLEC 100 L+T +PHV + C+EC Sbjct: 51 LKTLELPHVFVNCVEC 66 >5OHX-A nrPDB Length = 504 Score = 25.0 bits (53), Expect = 7.6, Method: Composition-based stats. Identities = 11/45 (24%), Positives = 25/45 (55%) Query: 16 KKIAIERIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIP 60 +K++ E+I TL+ L ++ R + + E + + +K + +IP Sbjct: 130 EKMSDEKISTLYALGAKIIRTPTEASWHSPEAHISVAQKLQKEIP 174 >3BNI-A nrPDB Length = 229 Score = 24.6 bits (52), Expect = 9.4, Method: Compositional matrix adjust. Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 3/34 (8%) Query: 18 IAIERIDTLFTLAERVARYSPDLAKRYVELALEI 51 +A+E DTL LA RVA PD ++ +E A E+ Sbjct: 187 VAVETADTLVQLAFRVA---PDGDEKIIEEAREL 217 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1X0T-A nrPDB 174 1e-44 2KI7-B nrPDB 153 2e-38 Sequences not found previously or not previously below threshold: 6AHU-K nrPDB 34 0.017 6AGB-K nrPDB 30 0.30 2YEQ-A nrPDB 27 2.1 1ZN9-A nrPDB 26 2.5 2PV0-B nrPDB 26 4.8 5UJM-E nrPDB 25 5.9 5UJ7-E nrPDB 25 6.1 6GWA-A nrPDB 25 6.3 5T6R-V nrPDB 25 6.6 2QRV-B nrPDB 25 7.0 5T2N-A nrPDB 25 8.4 3BX4-A nrPDB 25 8.9 3MMT-A nrPDB 25 9.4 CONVERGED! >1X0T-A nrPDB Length = 120 Score = 174 bits (440), Expect = 1e-44, Method: Composition-based stats. Identities = 120/120 (100%), Positives = 120/120 (100%) Query: 1 MVDIVKRRDWEKKEKKKIAIERIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIP 60 MVDIVKRRDWEKKEKKKIAIERIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIP Sbjct: 1 MVDIVKRRDWEKKEKKKIAIERIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIP 60 Query: 61 RKWKRRYCKRCHTFLIPGVNARVRLRTKRMPHVVITCLECGYIMRYPYLREVKQKRKKAT 120 RKWKRRYCKRCHTFLIPGVNARVRLRTKRMPHVVITCLECGYIMRYPYLREVKQKRKKAT Sbjct: 61 RKWKRRYCKRCHTFLIPGVNARVRLRTKRMPHVVITCLECGYIMRYPYLREVKQKRKKAT 120 >2KI7-B nrPDB Length = 123 Score = 153 bits (386), Expect = 2e-38, Method: Composition-based stats. Identities = 79/98 (80%), Positives = 90/98 (91%) Query: 11 EKKEKKKIAIERIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIPRKWKRRYCKR 70 EKKEKK+IA ERID LF+LAERV YSP+LAKRYVELAL +Q+KAKVKIPRKWKRRYCK+ Sbjct: 6 EKKEKKRIAKERIDILFSLAERVFPYSPELAKRYVELALLVQQKAKVKIPRKWKRRYCKK 65 Query: 71 CHTFLIPGVNARVRLRTKRMPHVVITCLECGYIMRYPY 108 CH FL+PG+NARVRLR KRMPH+V+ CLECG+IMRYPY Sbjct: 66 CHAFLVPGINARVRLRQKRMPHIVVKCLECGHIMRYPY 103 >6AHU-K nrPDB Length = 154 Score = 33.8 bits (76), Expect = 0.017, Method: Composition-based stats. Identities = 28/96 (29%), Positives = 43/96 (44%), Gaps = 4/96 (4%) Query: 15 KKKIAIERIDTLFTLAERVARYSPD---LAKRYVELALEIQKKAKVKIPRKWKRRYCKRC 71 K + A +R++ L+ A V P+ LA+ Y I K+ ++ KR C+ C Sbjct: 6 KDREAFQRLNFLYQAAHCVLAQDPENQALARFYCYTERTIAKRLVLRRDPSVKRTLCRGC 65 Query: 72 HTFLIPGVNARVRLRTKRMPH-VVITCLECGYIMRY 106 + L+PG+ R R R V TCL C R+ Sbjct: 66 SSLLVPGLTCTQRQRRCRGQRWTVQTCLTCQRSQRF 101 >6AGB-K nrPDB Length = 144 Score = 29.6 bits (65), Expect = 0.30, Method: Composition-based stats. Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 1/44 (2%) Query: 32 RVARYSPDLAKRYVELALEIQKKAKVKIPRKWKRRYCKRCHTFL 75 R ++P LA+ Y++ I KK K + KR CK+CH L Sbjct: 55 RTDAHTP-LARNYIKSMDLISKKTKTSLLPTIKRTICKKCHRLL 97 >2YEQ-A nrPDB Length = 527 Score = 26.9 bits (58), Expect = 2.1, Method: Composition-based stats. Identities = 8/24 (33%), Positives = 15/24 (62%) Query: 16 KKIAIERIDTLFTLAERVARYSPD 39 K +A E++D +F L + + Y P+ Sbjct: 136 KHMAKEKLDLVFHLGDYIYEYGPN 159 >1ZN9-A nrPDB Length = 180 Score = 26.5 bits (57), Expect = 2.5, Method: Composition-based stats. Identities = 13/42 (30%), Positives = 20/42 (47%) Query: 22 RIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIPRKW 63 RID + L R + P LA+ + I+K+ K+ P W Sbjct: 57 RIDYIAGLDSRGFLFGPSLAQELGLGCVLIRKRGKLPGPTLW 98 >2PV0-B nrPDB Length = 386 Score = 25.7 bits (55), Expect = 4.8, Method: Composition-based stats. Identities = 10/26 (38%), Positives = 13/26 (50%) Query: 46 ELALEIQKKAKVKIPRKWKRRYCKRC 71 EL+L Q K K+ KW + K C Sbjct: 342 ELSLLAQNKQSSKLAAKWPTKLVKNC 367 >5UJM-E nrPDB Length = 435 Score = 25.3 bits (54), Expect = 5.9, Method: Composition-based stats. Identities = 8/16 (50%), Positives = 12/16 (75%) Query: 85 LRTKRMPHVVITCLEC 100 L+T +PHV + C+EC Sbjct: 51 LKTLELPHVFVNCVEC 66 >5UJ7-E nrPDB Length = 284 Score = 25.3 bits (54), Expect = 6.1, Method: Composition-based stats. Identities = 8/16 (50%), Positives = 12/16 (75%) Query: 85 LRTKRMPHVVITCLEC 100 L+T +PHV + C+EC Sbjct: 51 LKTLELPHVFVNCVEC 66 >6GWA-A nrPDB Length = 299 Score = 25.3 bits (54), Expect = 6.3, Method: Composition-based stats. Identities = 11/29 (37%), Positives = 15/29 (51%) Query: 28 TLAERVARYSPDLAKRYVELALEIQKKAK 56 L +V Y PDL RY +AL ++ K Sbjct: 243 ALIGQVLPYLPDLQTRYAGIALWNRQADK 271 >5T6R-V nrPDB Length = 917 Score = 25.3 bits (54), Expect = 6.6, Method: Composition-based stats. Identities = 15/67 (22%), Positives = 29/67 (43%), Gaps = 10/67 (14%) Query: 59 IPRKWKRRYCKRCHTFLI-PGVNARVRLRTKRMPHVV---------ITCLECGYIMRYPY 108 IPR+ +C+ C FL PG R L ++ + + + ++ +I P+ Sbjct: 448 IPREANISFCRNCERFLQPPGQWIRAELESRELLAICLRRLKGLTKVRLVDASFIWTEPH 507 Query: 109 LREVKQK 115 R ++ K Sbjct: 508 SRRIRIK 514 >2QRV-B nrPDB Length = 230 Score = 25.3 bits (54), Expect = 7.0, Method: Composition-based stats. Identities = 10/26 (38%), Positives = 13/26 (50%) Query: 46 ELALEIQKKAKVKIPRKWKRRYCKRC 71 EL+L Q K K+ KW + K C Sbjct: 186 ELSLLAQNKQSSKLAAKWPTKLVKNC 211 >5T2N-A nrPDB Length = 296 Score = 24.9 bits (53), Expect = 8.4, Method: Composition-based stats. Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 2/42 (4%) Query: 63 WKRRYCKRCHTFLIPGVNARVRLRTKRMPHVVITCLECGYIM 104 WKR+ L+ G++ +R K + + +IT L CGYI+ Sbjct: 181 WKRKGGTHVGVQLVFGISQHIR--DKNLMNSLITYLGCGYIL 220 >3BX4-A nrPDB Length = 136 Score = 24.9 bits (53), Expect = 8.9, Method: Composition-based stats. Identities = 7/15 (46%), Positives = 10/15 (66%) Query: 61 RKWKRRYCKRCHTFL 75 RKW YC++ H F+ Sbjct: 116 RKWVNYYCEQMHAFV 130 >3MMT-A nrPDB Length = 347 Score = 24.6 bits (52), Expect = 9.4, Method: Composition-based stats. Identities = 20/98 (20%), Positives = 38/98 (38%), Gaps = 5/98 (5%) Query: 20 IERIDTLFTLAERVARYSPDLAKRYVELALEIQKKAKVKIPRKWKRRYCKRCH-TFLIPG 78 ER+ +TL R A++ +A A + + + + RY C L+P Sbjct: 125 RERLKDYYTLGARFAKWRAVIAID----AQTLPTRGAISQNAQALARYAALCQEAGLVPI 180 Query: 79 VNARVRLRTKRMPHVVITCLECGYIMRYPYLREVKQKR 116 V V + H + C E ++ + +E+ + R Sbjct: 181 VEPEVLMDGPSRQHSITRCFEVTKVVLHTVFKELFEAR 218 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.142 0.447 Lambda K H 0.267 0.0433 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,265,651 Number of Sequences: 36641 Number of extensions: 205850 Number of successful extensions: 846 Number of sequences better than 10.0: 29 Number of HSP's better than 10.0 without gapping: 26 Number of HSP's successfully gapped in prelim test: 19 Number of HSP's that attempted gapping in prelim test: 820 Number of HSP's gapped (non-prelim): 45 length of query: 120 length of database: 10,336,785 effective HSP length: 84 effective length of query: 36 effective length of database: 7,258,941 effective search space: 261321876 effective search space used: 261321876 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.3 bits) S2: 53 (24.9 bits)