BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O55057 (150 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5T4X-A nrPDB 313 2e-86 1KSH-B nrPDB 306 2e-84 3RBQ-A nrPDB 47 3e-06 4GOK-G nrPDB 47 4e-06 2W8S-A nrPDB 30 0.41 5ZGM-A nrPDB 27 4.9 5PAL-A nrPDB 26 6.2 6DAM-A nrPDB 26 8.1 >5T4X-A nrPDB Length = 170 Score = 313 bits (801), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 150/150 (100%), Positives = 150/150 (100%) Query: 1 MSAKDERARDILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS 60 MSAKDERARDILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS Sbjct: 21 MSAKDERARDILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS 80 Query: 61 RELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA 120 RELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA Sbjct: 81 RELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA 140 Query: 121 SVLTGNVIIETKFFDDDLLVSTSKVRLFYV 150 SVLTGNVIIETKFFDDDLLVSTSKVRLFYV Sbjct: 141 SVLTGNVIIETKFFDDDLLVSTSKVRLFYV 170 >1KSH-B nrPDB Length = 152 Score = 306 bits (785), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 147/150 (98%), Positives = 149/150 (99%) Query: 1 MSAKDERARDILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS 60 MSAKDERAR+ILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS Sbjct: 3 MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS 62 Query: 61 RELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA 120 RELNFSS EQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA Sbjct: 63 RELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA 122 Query: 121 SVLTGNVIIETKFFDDDLLVSTSKVRLFYV 150 SVLTGNVIIETKFFDDDLLVSTS+VRLFYV Sbjct: 123 SVLTGNVIIETKFFDDDLLVSTSRVRLFYV 152 >3RBQ-A nrPDB Length = 195 Score = 47.0 bits (110), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 27/99 (27%), Positives = 53/99 (53%), Gaps = 3/99 (3%) Query: 54 LKCKAVSRELNFSSAEQ-MEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAA 112 L+ + V + F+ ++ + FR+ ++ YF+ Q L+ + F FGF IP+S NT + + + Sbjct: 93 LRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFP 152 Query: 113 PESQMMPASVLTGNVIIETK--FFDDDLLVSTSKVRLFY 149 P S+ + + ++ ++ +F DD LV +K Y Sbjct: 153 PLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSY 191 >4GOK-G nrPDB Length = 240 Score = 46.6 bits (109), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 27/99 (27%), Positives = 53/99 (53%), Gaps = 3/99 (3%) Query: 54 LKCKAVSRELNFSSAEQ-MEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAA 112 L+ + V + F+ ++ + FR+ ++ YF+ Q L+ + F FGF IP+S NT + + + Sbjct: 138 LRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFP 197 Query: 113 PESQMMPASVLTGNVIIETK--FFDDDLLVSTSKVRLFY 149 P S+ + + ++ ++ +F DD LV +K Y Sbjct: 198 PLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSY 236 >2W8S-A nrPDB Length = 543 Score = 30.0 bits (66), Expect = 0.41, Method: Composition-based stats. Identities = 34/140 (24%), Positives = 56/140 (40%), Gaps = 26/140 (18%) Query: 2 SAKDERAR---DILRGFK------------LNWMNLRDAETGKILWQGTEDLSVPGVEHE 46 SA+D R DI+ GF+ W+ A+ G L + ED+ +P EH Sbjct: 145 SARDPRFTVLGDIMDGFRSVGAFEPNMEGYFGWV----AQNGFELPENREDIWLPEGEHS 200 Query: 47 ARVPKKILKCKAVSRELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQ 106 VP K + +E + S+ F Y KG+ + +F G+ P+ Sbjct: 201 --VPGATDKPSRIPKEFSDSTF-----FTERALTYLKGRDGKPFFLHLGYYRPHPPFVAS 253 Query: 107 SLIEAAPESQMMPASVLTGN 126 + A +++ MPA + N Sbjct: 254 APYHAMYKAEDMPAPIRAEN 273 >5ZGM-A nrPDB Length = 110 Score = 26.6 bits (57), Expect = 4.9, Method: Compositional matrix adjust. Identities = 11/33 (33%), Positives = 18/33 (54%) Query: 5 DERARDILRGFKLNWMNLRDAETGKILWQGTED 37 +E + +L+GF + +L D ET +L G D Sbjct: 61 EEELKSVLKGFSAHGRDLSDTETKALLAAGDSD 93 >5PAL-A nrPDB Length = 109 Score = 26.2 bits (56), Expect = 6.2, Method: Compositional matrix adjust. Identities = 11/32 (34%), Positives = 17/32 (53%) Query: 6 ERARDILRGFKLNWMNLRDAETGKILWQGTED 37 E + +L+GF + +L D ET +L G D Sbjct: 61 EELKGVLKGFSAHGRDLNDTETKALLAAGDSD 92 >6DAM-A nrPDB Length = 617 Score = 25.8 bits (55), Expect = 8.1, Method: Composition-based stats. Identities = 10/17 (58%), Positives = 11/17 (64%) Query: 24 DAETGKILWQGTEDLSV 40 DA TGKI+W E SV Sbjct: 489 DATTGKIIWSNKEQFSV 505 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5T4X-A nrPDB 278 5e-76 1KSH-B nrPDB 273 3e-74 4GOK-G nrPDB 159 5e-40 3RBQ-A nrPDB 156 3e-39 Sequences not found previously or not previously below threshold: 2W8S-A nrPDB 31 0.20 5DND-A nrPDB 30 0.38 3SRE-A nrPDB 27 2.4 4GF2-A nrPDB 27 2.7 CONVERGED! >5T4X-A nrPDB Length = 170 Score = 278 bits (712), Expect = 5e-76, Method: Composition-based stats. Identities = 150/150 (100%), Positives = 150/150 (100%) Query: 1 MSAKDERARDILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS 60 MSAKDERARDILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS Sbjct: 21 MSAKDERARDILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS 80 Query: 61 RELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA 120 RELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA Sbjct: 81 RELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA 140 Query: 121 SVLTGNVIIETKFFDDDLLVSTSKVRLFYV 150 SVLTGNVIIETKFFDDDLLVSTSKVRLFYV Sbjct: 141 SVLTGNVIIETKFFDDDLLVSTSKVRLFYV 170 >1KSH-B nrPDB Length = 152 Score = 273 bits (697), Expect = 3e-74, Method: Composition-based stats. Identities = 147/150 (98%), Positives = 149/150 (99%) Query: 1 MSAKDERARDILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS 60 MSAKDERAR+ILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS Sbjct: 3 MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVS 62 Query: 61 RELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA 120 RELNFSS EQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA Sbjct: 63 RELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPA 122 Query: 121 SVLTGNVIIETKFFDDDLLVSTSKVRLFYV 150 SVLTGNVIIETKFFDDDLLVSTS+VRLFYV Sbjct: 123 SVLTGNVIIETKFFDDDLLVSTSRVRLFYV 152 >4GOK-G nrPDB Length = 240 Score = 159 bits (402), Expect = 5e-40, Method: Composition-based stats. Identities = 27/99 (27%), Positives = 53/99 (53%), Gaps = 3/99 (3%) Query: 54 LKCKAVSRELNFSSAEQ-MEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAA 112 L+ + V + F+ ++ + FR+ ++ YF+ Q L+ + F FGF IP+S NT + + + Sbjct: 138 LRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFP 197 Query: 113 PESQMMPASVLTGNVIIETK--FFDDDLLVSTSKVRLFY 149 P S+ + + ++ ++ +F DD LV +K Y Sbjct: 198 PLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSY 236 >3RBQ-A nrPDB Length = 195 Score = 156 bits (395), Expect = 3e-39, Method: Composition-based stats. Identities = 27/99 (27%), Positives = 53/99 (53%), Gaps = 3/99 (3%) Query: 54 LKCKAVSRELNFSSAEQ-MEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAA 112 L+ + V + F+ ++ + FR+ ++ YF+ Q L+ + F FGF IP+S NT + + + Sbjct: 93 LRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFP 152 Query: 113 PESQMMPASVLTGNVIIETK--FFDDDLLVSTSKVRLFY 149 P S+ + + ++ ++ +F DD LV +K Y Sbjct: 153 PLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSY 191 >2W8S-A nrPDB Length = 543 Score = 31.1 bits (69), Expect = 0.20, Method: Composition-based stats. Identities = 35/145 (24%), Positives = 57/145 (39%), Gaps = 26/145 (17%) Query: 2 SAKDERAR---DILRGFK------------LNWMNLRDAETGKILWQGTEDLSVPGVEHE 46 SA+D R DI+ GF+ W+ A+ G L + ED+ +P EH Sbjct: 145 SARDPRFTVLGDIMDGFRSVGAFEPNMEGYFGWV----AQNGFELPENREDIWLPEGEHS 200 Query: 47 ARVPKKILKCKAVSRELNFSSAEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQ 106 VP K + +E + S+ F Y KG+ + +F G+ P+ Sbjct: 201 --VPGATDKPSRIPKEFSDSTF-----FTERALTYLKGRDGKPFFLHLGYYRPHPPFVAS 253 Query: 107 SLIEAAPESQMMPASVLTGNVIIET 131 + A +++ MPA + N E Sbjct: 254 APYHAMYKAEDMPAPIRAENPDAEA 278 >5DND-A nrPDB Length = 588 Score = 30.0 bits (66), Expect = 0.38, Method: Composition-based stats. Identities = 17/77 (22%), Positives = 35/77 (45%), Gaps = 11/77 (14%) Query: 33 QGTED--LSVPGVEHEARVPKKILKCKAVSRELNFSSAEQMEKFRLEQKVYFKGQCLEEW 90 QG D L++P H RV +L+C+ + + + + + ++ ++ Y + Q Sbjct: 77 QGLPDHALALPPASHGPRVLYTVLECQPLLDSSDMTIDDWIRIAKIIERHYEQYQ----- 131 Query: 91 FFEFGFVIPNSTNTWQS 107 GFV+ + +T S Sbjct: 132 ----GFVVIHGADTMAS 144 >3SRE-A nrPDB Length = 355 Score = 27.3 bits (59), Expect = 2.4, Method: Composition-based stats. Identities = 15/75 (20%), Positives = 24/75 (32%), Gaps = 11/75 (14%) Query: 51 KKILKCKAVSRELNFSSAEQM----EKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQ 106 K +L K + +L S + + E F YF L+ W G W Sbjct: 151 KSLLHLKTIRHKLLPSVNDIVAVGPEHFYATNDHYFIDPYLKSWEMHLGL-------AWS 203 Query: 107 SLIEAAPESQMMPAS 121 + +P + A Sbjct: 204 FVTYYSPNDVRVVAE 218 >4GF2-A nrPDB Length = 615 Score = 27.3 bits (59), Expect = 2.7, Method: Composition-based stats. Identities = 26/111 (23%), Positives = 49/111 (44%), Gaps = 7/111 (6%) Query: 43 VEHEARVPKKILKCKAVSRELNFSSAEQMEKFRLEQKVYF---KGQCLEEWFFE--FGFV 97 V H+ + K+ A ++N E E F +E+K F K +C+E +E FG Sbjct: 156 VNHKGNIAKECAIIPAEEPQINLWIKEWNENFLMEKKRLFLNIKDKCVENKKYEACFGGC 215 Query: 98 -IPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSKVRL 147 +P S+ T + ++ + +++ N ++ F +DL +K L Sbjct: 216 RLPCSSYT-SFMKKSKTQMEVLTNLYKKKNSGVDKNNFLNDLFKKNNKNDL 265 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.138 0.399 Lambda K H 0.267 0.0425 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,883,705 Number of Sequences: 36641 Number of extensions: 216783 Number of successful extensions: 456 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 436 Number of HSP's gapped (non-prelim): 27 length of query: 150 length of database: 10,336,785 effective HSP length: 88 effective length of query: 62 effective length of database: 7,112,377 effective search space: 440967374 effective search space used: 440967374 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 55 (25.7 bits)