BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O43813 (399 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3E73-A nrPDB 832 0.0 3T33-A nrPDB 239 1e-63 1D9Z-A nrPDB 32 0.67 5U7V-A nrPDB 30 2.1 3QT4-A nrPDB 29 3.8 4QN6-A nrPDB 28 7.9 5HUM-A nrPDB 28 8.1 3SBP-A nrPDB 28 8.2 >3E73-A nrPDB Length = 411 Score = 832 bits (2148), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/399 (100%), Positives = 399/399 (100%) Query: 1 MAQRAFPNPYADYNKSLAEGYFDAAGRLTPEFSQRLTNKIRELLQQMERGLKSADPRDGT 60 MAQRAFPNPYADYNKSLAEGYFDAAGRLTPEFSQRLTNKIRELLQQMERGLKSADPRDGT Sbjct: 13 MAQRAFPNPYADYNKSLAEGYFDAAGRLTPEFSQRLTNKIRELLQQMERGLKSADPRDGT 72 Query: 61 GYTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQSLNCLTKRSITFLCGDAGPLAVAAVL 120 GYTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQSLNCLTKRSITFLCGDAGPLAVAAVL Sbjct: 73 GYTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQSLNCLTKRSITFLCGDAGPLAVAAVL 132 Query: 121 YHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYGRIGYIYALLFVNKNFGVEKIPQSHIQ 180 YHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYGRIGYIYALLFVNKNFGVEKIPQSHIQ Sbjct: 133 YHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYGRIGYIYALLFVNKNFGVEKIPQSHIQ 192 Query: 181 QICETILTSGENLARKRNFTAKSPLMYEWYQEYYVGAAHGLAGIYYYLMQPSLQVSQGKL 240 QICETILTSGENLARKRNFTAKSPLMYEWYQEYYVGAAHGLAGIYYYLMQPSLQVSQGKL Sbjct: 193 QICETILTSGENLARKRNFTAKSPLMYEWYQEYYVGAAHGLAGIYYYLMQPSLQVSQGKL 252 Query: 241 HSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVHWCHGAPGVIYMLIQAYKVFREEKYLC 300 HSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVHWCHGAPGVIYMLIQAYKVFREEKYLC Sbjct: 253 HSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVHWCHGAPGVIYMLIQAYKVFREEKYLC 312 Query: 301 DAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLTLYNLTQDMKYLYRACKFAEWCLEYGE 360 DAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLTLYNLTQDMKYLYRACKFAEWCLEYGE Sbjct: 313 DAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLTLYNLTQDMKYLYRACKFAEWCLEYGE 372 Query: 361 HGCRTPDTPFSLFEGMAGTIYFLADLLVPTKARFPAFEL 399 HGCRTPDTPFSLFEGMAGTIYFLADLLVPTKARFPAFEL Sbjct: 373 HGCRTPDTPFSLFEGMAGTIYFLADLLVPTKARFPAFEL 411 >3T33-A nrPDB Length = 415 Score = 239 bits (610), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 146/373 (39%), Positives = 206/373 (55%), Gaps = 23/373 (6%) Query: 36 LTNKIRELLQQMERGLKSADPRDGTGYTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQ- 94 L+ K + + + ER K RD YTG G A L Y V + L+L V+ Sbjct: 57 LSIKDKVVWETWERSGKRV--RDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEAC 114 Query: 95 SLNCLTKRSITFLCGDAGPLAVAAVLYHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYG 154 + +TF+CG AG A+ AV + +++ + + R + ++ P E+LYG Sbjct: 115 DVASRDSERVTFICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGI-RLPSDLPYELLYG 173 Query: 155 RIGYIYALLFVNKNFGVEKIPQSHIQQICETILTSGENLARKRNFTAKSPLMYEWYQEYY 214 R GY++A LF+NK+ G E I ++ + E I +G L K PLMYEW+ + Y Sbjct: 174 RAGYLWACLFLNKHIGQESISSERMRSVVEEIFRAGRQLGNK----GTCPLMYEWHGKRY 229 Query: 215 VGAAHGLAGIYYYLMQPSLQVSQGKLHSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVH 274 GAAHGLAGI LM L+ + K VK ++ Y+ Q +FPSGNY G D LVH Sbjct: 230 WGAAHGLAGIMNVLMHTELEPDEIKD---VKGTLSYMIQNRFPSGNYLSSEGSKSDRLVH 286 Query: 275 WCHGAPGVIYMLIQAYKVFREEKYLCDAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLT 334 WCHGAPGV L++A +V+ ++++ A + +V+W GLLK+ G+CHG +GN Y FL+ Sbjct: 287 WCHGAPGVALTLVKAAQVYNTKEFVEAAMEAGEVVWSRGLLKR-VGICHGISGNTYVFLS 345 Query: 335 LYNLTQDMKYLYRACKFAEWCLEYGE--------HGCRTPDTPFSLFEGMAGTIYFLADL 386 LY LT++ KYLYRA FA + L+ E HG D PFSLFEG+ G Y L D+ Sbjct: 346 LYRLTRNPKYLYRAKAFASFLLDKSEKLISEGQMHG---GDRPFSLFEGIGGMAYMLLDM 402 Query: 387 LVPTKARFPAFEL 399 PT+A FP +EL Sbjct: 403 NDPTQALFPGYEL 415 >1D9Z-A nrPDB Length = 657 Score = 31.6 bits (70), Expect = 0.67, Method: Compositional matrix adjust. Identities = 26/83 (31%), Positives = 36/83 (43%), Gaps = 13/83 (15%) Query: 5 AFPNPYA-DYNKSLAEGYFDAAGRLTPEFSQR--------LTNKIRELLQQMERGLKSAD 55 A P PY +++ + E G L P R L +IRE +++ ER L + Sbjct: 393 ATPGPYELEHSPGVVEQIIRPTGLLDPTIDVRPTKGQIDDLIGEIRERVERNERTLVTTL 452 Query: 56 PR----DGTGYTGWAGIAVLYLH 74 + D T Y AGI V YLH Sbjct: 453 TKKMAEDLTDYLKEAGIKVAYLH 475 >5U7V-A nrPDB Length = 426 Score = 30.0 bits (66), Expect = 2.1, Method: Compositional matrix adjust. Identities = 13/26 (50%), Positives = 17/26 (65%) Query: 296 EKYLCDAYQCADVIWQYGLLKKGYGL 321 EK+ Y C D+I+QY LL G+GL Sbjct: 348 EKFNIVPYACMDLIYQYELLVDGFGL 373 >3QT4-A nrPDB Length = 329 Score = 28.9 bits (63), Expect = 3.8, Method: Compositional matrix adjust. Identities = 21/68 (30%), Positives = 29/68 (42%), Gaps = 14/68 (20%) Query: 64 GWAGIAVLYLHLYDVFGDPAYLQLAHG-----YVKQSLNCLT---------KRSITFLCG 109 GW A Y+H Y + + AY A G QS+ L+ + S+ G Sbjct: 182 GWMDSAFSYIHDYGIMSESAYPYEAQGDYCRFDSSQSVTTLSGYYDLPSGDENSLADAVG 241 Query: 110 DAGPLAVA 117 AGP+AVA Sbjct: 242 QAGPVAVA 249 >4QN6-A nrPDB Length = 391 Score = 28.1 bits (61), Expect = 7.9, Method: Compositional matrix adjust. Identities = 15/48 (31%), Positives = 24/48 (50%) Query: 62 YTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQSLNCLTKRSITFLCG 109 ++G++G + Y + F Y++L G K+S T SI LCG Sbjct: 324 WSGYSGAFIDYWANKECFNPCFYVELIRGRPKESSVLWTSNSIVALCG 371 >5HUM-A nrPDB Length = 391 Score = 27.7 bits (60), Expect = 8.1, Method: Compositional matrix adjust. Identities = 15/48 (31%), Positives = 24/48 (50%) Query: 62 YTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQSLNCLTKRSITFLCG 109 ++G++G + Y + F Y++L G K+S T SI LCG Sbjct: 324 WSGYSGAFIDYWANKECFNPCFYVELIRGRPKESSVLWTSNSIVALCG 371 >3SBP-A nrPDB Length = 638 Score = 27.7 bits (60), Expect = 8.2, Method: Compositional matrix adjust. Identities = 20/51 (39%), Positives = 26/51 (50%), Gaps = 6/51 (11%) Query: 8 NPYADYNKSLAEGYFDAAGRLTPEFSQRLTNKIRELLQQMERGLKSADPRD 58 NP+ N S YF AAG+L+P S +K+ +L K ADPRD Sbjct: 324 NPHG-CNTSSDGKYFIAAGKLSPTCSMIAIDKLPDLFAG-----KLADPRD 368 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3E73-A nrPDB 697 0.0 3T33-A nrPDB 559 e-160 Sequences not found previously or not previously below threshold: 2EMT-A nrPDB 29 4.0 1VJT-A nrPDB 29 4.2 5GK9-A nrPDB 29 4.7 3U95-A nrPDB 28 9.3 CONVERGED! >3E73-A nrPDB Length = 411 Score = 697 bits (1798), Expect = 0.0, Method: Composition-based stats. Identities = 399/399 (100%), Positives = 399/399 (100%) Query: 1 MAQRAFPNPYADYNKSLAEGYFDAAGRLTPEFSQRLTNKIRELLQQMERGLKSADPRDGT 60 MAQRAFPNPYADYNKSLAEGYFDAAGRLTPEFSQRLTNKIRELLQQMERGLKSADPRDGT Sbjct: 13 MAQRAFPNPYADYNKSLAEGYFDAAGRLTPEFSQRLTNKIRELLQQMERGLKSADPRDGT 72 Query: 61 GYTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQSLNCLTKRSITFLCGDAGPLAVAAVL 120 GYTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQSLNCLTKRSITFLCGDAGPLAVAAVL Sbjct: 73 GYTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQSLNCLTKRSITFLCGDAGPLAVAAVL 132 Query: 121 YHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYGRIGYIYALLFVNKNFGVEKIPQSHIQ 180 YHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYGRIGYIYALLFVNKNFGVEKIPQSHIQ Sbjct: 133 YHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYGRIGYIYALLFVNKNFGVEKIPQSHIQ 192 Query: 181 QICETILTSGENLARKRNFTAKSPLMYEWYQEYYVGAAHGLAGIYYYLMQPSLQVSQGKL 240 QICETILTSGENLARKRNFTAKSPLMYEWYQEYYVGAAHGLAGIYYYLMQPSLQVSQGKL Sbjct: 193 QICETILTSGENLARKRNFTAKSPLMYEWYQEYYVGAAHGLAGIYYYLMQPSLQVSQGKL 252 Query: 241 HSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVHWCHGAPGVIYMLIQAYKVFREEKYLC 300 HSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVHWCHGAPGVIYMLIQAYKVFREEKYLC Sbjct: 253 HSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVHWCHGAPGVIYMLIQAYKVFREEKYLC 312 Query: 301 DAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLTLYNLTQDMKYLYRACKFAEWCLEYGE 360 DAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLTLYNLTQDMKYLYRACKFAEWCLEYGE Sbjct: 313 DAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLTLYNLTQDMKYLYRACKFAEWCLEYGE 372 Query: 361 HGCRTPDTPFSLFEGMAGTIYFLADLLVPTKARFPAFEL 399 HGCRTPDTPFSLFEGMAGTIYFLADLLVPTKARFPAFEL Sbjct: 373 HGCRTPDTPFSLFEGMAGTIYFLADLLVPTKARFPAFEL 411 >3T33-A nrPDB Length = 415 Score = 559 bits (1440), Expect = e-160, Method: Composition-based stats. Identities = 146/373 (39%), Positives = 206/373 (55%), Gaps = 23/373 (6%) Query: 36 LTNKIRELLQQMERGLKSADPRDGTGYTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQ- 94 L+ K + + + ER K RD YTG G A L Y V + L+L V+ Sbjct: 57 LSIKDKVVWETWERSGKRV--RDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEAC 114 Query: 95 SLNCLTKRSITFLCGDAGPLAVAAVLYHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYG 154 + +TF+CG AG A+ AV + +++ + + R + ++ P E+LYG Sbjct: 115 DVASRDSERVTFICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGI-RLPSDLPYELLYG 173 Query: 155 RIGYIYALLFVNKNFGVEKIPQSHIQQICETILTSGENLARKRNFTAKSPLMYEWYQEYY 214 R GY++A LF+NK+ G E I ++ + E I +G L K PLMYEW+ + Y Sbjct: 174 RAGYLWACLFLNKHIGQESISSERMRSVVEEIFRAGRQLGNK----GTCPLMYEWHGKRY 229 Query: 215 VGAAHGLAGIYYYLMQPSLQVSQGKLHSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVH 274 GAAHGLAGI LM L+ + K VK ++ Y+ Q +FPSGNY G D LVH Sbjct: 230 WGAAHGLAGIMNVLMHTELEPDEIKD---VKGTLSYMIQNRFPSGNYLSSEGSKSDRLVH 286 Query: 275 WCHGAPGVIYMLIQAYKVFREEKYLCDAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLT 334 WCHGAPGV L++A +V+ ++++ A + +V+W GLLK+ G+CHG +GN Y FL+ Sbjct: 287 WCHGAPGVALTLVKAAQVYNTKEFVEAAMEAGEVVWSRGLLKR-VGICHGISGNTYVFLS 345 Query: 335 LYNLTQDMKYLYRACKFAEWCLEYGE--------HGCRTPDTPFSLFEGMAGTIYFLADL 386 LY LT++ KYLYRA FA + L+ E HG D PFSLFEG+ G Y L D+ Sbjct: 346 LYRLTRNPKYLYRAKAFASFLLDKSEKLISEGQMHG---GDRPFSLFEGIGGMAYMLLDM 402 Query: 387 LVPTKARFPAFEL 399 PT+A FP +EL Sbjct: 403 NDPTQALFPGYEL 415 >2EMT-A nrPDB Length = 322 Score = 28.8 bits (63), Expect = 4.0, Method: Composition-based stats. Identities = 15/48 (31%), Positives = 23/48 (47%), Gaps = 3/48 (6%) Query: 1 MAQRAFPNPYADYNKSLAEGYFDAAGRLTPEFSQRLTNKIRELLQQME 48 +A A Y DYNK + + + A RL P QR+ + + +Q E Sbjct: 127 LASYAVQAKYGDYNKEIHKPGYLANDRLLP---QRVLEQHKLTKEQWE 171 >1VJT-A nrPDB Length = 483 Score = 28.8 bits (63), Expect = 4.2, Method: Composition-based stats. Identities = 9/30 (30%), Positives = 13/30 (43%) Query: 209 WYQEYYVGAAHGLAGIYYYLMQPSLQVSQG 238 W VG HG+AG+Y + L + Sbjct: 184 WTGANIVGFCHGVAGVYEVFEKLDLDPEEV 213 >5GK9-A nrPDB Length = 276 Score = 28.8 bits (63), Expect = 4.7, Method: Composition-based stats. Identities = 12/51 (23%), Positives = 20/51 (39%), Gaps = 6/51 (11%) Query: 146 HAPNEMLYGRIGYIYALLFVNKNFGVEKIPQSHIQQIC------ETILTSG 190 H+P Y R+G +Y F K + I + H+ + + I G Sbjct: 17 HSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKG 67 >3U95-A nrPDB Length = 477 Score = 27.7 bits (60), Expect = 9.3, Method: Composition-based stats. Identities = 8/30 (26%), Positives = 13/30 (43%) Query: 209 WYQEYYVGAAHGLAGIYYYLMQPSLQVSQG 238 W +G HG+AG+Y + L + Sbjct: 172 WTGANIIGFCHGVAGVYEVFERLGLDPEEV 201 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.322 0.140 0.413 Lambda K H 0.267 0.0427 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 17,160,367 Number of Sequences: 36641 Number of extensions: 728025 Number of successful extensions: 1677 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 1642 Number of HSP's gapped (non-prelim): 23 length of query: 399 length of database: 10,336,785 effective HSP length: 99 effective length of query: 300 effective length of database: 6,709,326 effective search space: 2012797800 effective search space used: 2012797800 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 60 (27.7 bits)