BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O43809 (227 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3BHO-A nrPDB 428 e-120 2DU4-A nrPDB 33 0.095 3DGT-A nrPDB 33 0.12 4ZBP-A nrPDB 30 0.72 3U6F-B nrPDB 28 3.0 5K1R-A nrPDB 28 4.7 4E1J-A nrPDB 27 5.2 5VVE-A nrPDB 27 5.7 2FGT-A nrPDB 27 7.3 >3BHO-A nrPDB Length = 208 Score = 428 bits (1100), Expect = e-120, Method: Compositional matrix adjust. Identities = 208/208 (100%), Positives = 208/208 (100%) Query: 20 FGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRT 79 FGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRT Sbjct: 1 FGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRT 60 Query: 80 VEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDW 139 VEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDW Sbjct: 61 VEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDW 120 Query: 140 VIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLF 199 VIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLF Sbjct: 121 VIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLF 180 Query: 200 ELYDNAPGYGPIISSLPQLLSRFNFIYN 227 ELYDNAPGYGPIISSLPQLLSRFNFIYN Sbjct: 181 ELYDNAPGYGPIISSLPQLLSRFNFIYN 208 >2DU4-A nrPDB Length = 534 Score = 33.1 bits (74), Expect = 0.095, Method: Compositional matrix adjust. Identities = 20/62 (32%), Positives = 31/62 (50%), Gaps = 8/62 (12%) Query: 29 KPLTLERTIN-LYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVH 87 K + ER+ N LYP ++FG + PL+ A QR+RE + IG V +++ Sbjct: 24 KEILAERSPNELYPRVGFSFGKEHPLF-------ATIQRLREAYLSIGFSEVVNPLIVED 76 Query: 88 EH 89 H Sbjct: 77 VH 78 >3DGT-A nrPDB Length = 280 Score = 32.7 bits (73), Expect = 0.12, Method: Compositional matrix adjust. Identities = 27/108 (25%), Positives = 40/108 (37%) Query: 102 TFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYI 161 T PGG N G EVE + + + +G L D GNW E + + Sbjct: 34 TGTSYPGGAGNWGTGEVESMTSSTSNVSLDGNGDLLITPRRDASGNWTSGRIETTRTDFQ 93 Query: 162 PAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYG 209 P K + +L + + A + ++ AP Y N PG G Sbjct: 94 PPAGGKLRVEARLQMPNVTGDAAAGYWPAFWMLGAPFRGNYQNWPGVG 141 >4ZBP-A nrPDB Length = 310 Score = 30.4 bits (67), Expect = 0.72, Method: Compositional matrix adjust. Identities = 21/65 (32%), Positives = 36/65 (55%), Gaps = 11/65 (16%) Query: 82 GVLIVHEHRLPHVLLLQLGTTFFK------LPGGELNPGEDEVEGLKRLMTEILGRQDGV 135 G L+++++ VL++Q + FFK LP G +N GED G+ R + E + G+ Sbjct: 135 GALVINKNT-KEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEE----ETGI 189 Query: 136 LQDWV 140 + D+V Sbjct: 190 IADFV 194 >3U6F-B nrPDB Length = 314 Score = 28.1 bits (61), Expect = 3.0, Method: Compositional matrix adjust. Identities = 11/26 (42%), Positives = 15/26 (57%) Query: 107 PGGELNPGEDEVEGLKRLMTEILGRQ 132 PG +PG E+ GL + E+ GRQ Sbjct: 73 PGKACSPGASEITGLSKAELEVQGRQ 98 >5K1R-A nrPDB Length = 480 Score = 27.7 bits (60), Expect = 4.7, Method: Compositional matrix adjust. Identities = 11/23 (47%), Positives = 13/23 (56%) Query: 141 IDDCIGNWWRPNFEPPQYPYIPA 163 +D C+G W P E YP IPA Sbjct: 232 VDGCLGGWMLPWGEALGYPDIPA 254 >4E1J-A nrPDB Length = 520 Score = 27.3 bits (59), Expect = 5.2, Method: Compositional matrix adjust. Identities = 18/43 (41%), Positives = 21/43 (48%), Gaps = 4/43 (9%) Query: 92 PHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDG 134 P +L GT F L LN G+D V RL+T I R DG Sbjct: 279 PGMLKSTYGTGCFAL----LNTGKDMVRSKNRLLTTIAYRLDG 317 >5VVE-A nrPDB Length = 258 Score = 27.3 bits (59), Expect = 5.7, Method: Compositional matrix adjust. Identities = 13/39 (33%), Positives = 23/39 (58%) Query: 30 PLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMR 68 PL E NL P+ +Y G +E + +K +SVA++ + + Sbjct: 220 PLVYELDENLKPIKHYYLGDQELIQQKINSVASQASKKK 258 >2FGT-A nrPDB Length = 417 Score = 26.9 bits (58), Expect = 7.3, Method: Compositional matrix adjust. Identities = 15/65 (23%), Positives = 28/65 (43%), Gaps = 3/65 (4%) Query: 148 WWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAV---PKNYKLVAAPLFELYDN 204 W +FE + +I + K +KK++LV ++ + V NY+ + L N Sbjct: 104 WSNQSFEYSSFDHILIPFNETKANKKIYLVSYSKQLILEVTVESANYRNIMNDLKNRQSN 163 Query: 205 APGYG 209 P + Sbjct: 164 MPAFS 168 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3BHO-A nrPDB 447 e-126 Sequences not found previously or not previously below threshold: 2DU4-A nrPDB 36 0.017 3DGT-A nrPDB 31 0.29 4ZBP-A nrPDB 30 1.1 2CTZ-A nrPDB 28 2.7 5VVE-A nrPDB 28 3.3 3U6F-B nrPDB 28 3.3 2DU7-A nrPDB 28 3.8 5K1R-A nrPDB 28 4.3 4GN1-A nrPDB 27 6.3 2ISA-A nrPDB 27 6.4 4E1J-A nrPDB 26 8.8 CONVERGED! >3BHO-A nrPDB Length = 208 Score = 447 bits (1149), Expect = e-126, Method: Composition-based stats. Identities = 208/208 (100%), Positives = 208/208 (100%) Query: 20 FGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRT 79 FGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRT Sbjct: 1 FGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRT 60 Query: 80 VEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDW 139 VEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDW Sbjct: 61 VEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDW 120 Query: 140 VIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLF 199 VIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLF Sbjct: 121 VIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLF 180 Query: 200 ELYDNAPGYGPIISSLPQLLSRFNFIYN 227 ELYDNAPGYGPIISSLPQLLSRFNFIYN Sbjct: 181 ELYDNAPGYGPIISSLPQLLSRFNFIYN 208 >2DU4-A nrPDB Length = 534 Score = 35.7 bits (81), Expect = 0.017, Method: Composition-based stats. Identities = 20/62 (32%), Positives = 31/62 (50%), Gaps = 8/62 (12%) Query: 29 KPLTLERTIN-LYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVH 87 K + ER+ N LYP ++FG + PL+ A QR+RE + IG V +++ Sbjct: 24 KEILAERSPNELYPRVGFSFGKEHPLF-------ATIQRLREAYLSIGFSEVVNPLIVED 76 Query: 88 EH 89 H Sbjct: 77 VH 78 >3DGT-A nrPDB Length = 280 Score = 31.5 bits (70), Expect = 0.29, Method: Composition-based stats. Identities = 28/112 (25%), Positives = 44/112 (39%), Gaps = 3/112 (2%) Query: 100 GTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYP 159 GT++ PGG N G EVE + + + +G L D GNW E + Sbjct: 35 GTSY---PGGAGNWGTGEVESMTSSTSNVSLDGNGDLLITPRRDASGNWTSGRIETTRTD 91 Query: 160 YIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPI 211 + P K + +L + + A + ++ AP Y N PG G + Sbjct: 92 FQPPAGGKLRVEARLQMPNVTGDAAAGYWPAFWMLGAPFRGNYQNWPGVGEL 143 >4ZBP-A nrPDB Length = 310 Score = 29.6 bits (65), Expect = 1.1, Method: Composition-based stats. Identities = 21/65 (32%), Positives = 36/65 (55%), Gaps = 11/65 (16%) Query: 82 GVLIVHEHRLPHVLLLQLGTTFFK------LPGGELNPGEDEVEGLKRLMTEILGRQDGV 135 G L+++++ VL++Q + FFK LP G +N GED G+ R + E + G+ Sbjct: 135 GALVINKNT-KEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEE----ETGI 189 Query: 136 LQDWV 140 + D+V Sbjct: 190 IADFV 194 >2CTZ-A nrPDB Length = 421 Score = 28.4 bits (62), Expect = 2.7, Method: Composition-based stats. Identities = 10/25 (40%), Positives = 16/25 (64%) Query: 27 QTKPLTLERTINLYPLTNYTFGTKE 51 + +P TL R + +YP T+Y F + E Sbjct: 13 EPEPTTLSRQVPIYPTTSYVFKSPE 37 >5VVE-A nrPDB Length = 258 Score = 28.0 bits (61), Expect = 3.3, Method: Composition-based stats. Identities = 13/39 (33%), Positives = 23/39 (58%) Query: 30 PLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMR 68 PL E NL P+ +Y G +E + +K +SVA++ + + Sbjct: 220 PLVYELDENLKPIKHYYLGDQELIQQKINSVASQASKKK 258 >3U6F-B nrPDB Length = 314 Score = 28.0 bits (61), Expect = 3.3, Method: Composition-based stats. Identities = 11/26 (42%), Positives = 15/26 (57%) Query: 107 PGGELNPGEDEVEGLKRLMTEILGRQ 132 PG +PG E+ GL + E+ GRQ Sbjct: 73 PGKACSPGASEITGLSKAELEVQGRQ 98 >2DU7-A nrPDB Length = 549 Score = 28.0 bits (61), Expect = 3.8, Method: Composition-based stats. Identities = 25/110 (22%), Positives = 53/110 (48%), Gaps = 15/110 (13%) Query: 26 QQTKPLTLERTI-NLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVL 84 ++T+ L ++ I N YP +P+Y K V +R+R+ + ++G + V+ Sbjct: 31 RETRALIKDKHIDNKYPRL-------KPVYGKPHPVMETIERLRQAYLRMGFEEMINPVI 83 Query: 85 I----VHEHRLPHVLLLQLGTTFF--KLPGGELNPGEDEVEGLKRLMTEI 128 + +++ P + + L F+ LP ++ G ++VE +K L +I Sbjct: 84 VDEMEIYKQFGPEAMAV-LDRCFYLAGLPRPDVGLGNEKVEIIKNLGIDI 132 >5K1R-A nrPDB Length = 480 Score = 27.6 bits (60), Expect = 4.3, Method: Composition-based stats. Identities = 12/26 (46%), Positives = 15/26 (57%), Gaps = 1/26 (3%) Query: 139 WV-IDDCIGNWWRPNFEPPQYPYIPA 163 W+ +D C+G W P E YP IPA Sbjct: 229 WLHVDGCLGGWMLPWGEALGYPDIPA 254 >4GN1-A nrPDB Length = 255 Score = 27.3 bits (59), Expect = 6.3, Method: Composition-based stats. Identities = 12/38 (31%), Positives = 23/38 (60%) Query: 166 TKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYD 203 T+ ++K +F+ ++++ ALF P+NY L E+ D Sbjct: 76 TRDSQNKLIFMERIEKYALFKNPQNYLLGKKETAEMAD 113 >2ISA-A nrPDB Length = 483 Score = 27.3 bits (59), Expect = 6.4, Method: Composition-based stats. Identities = 14/41 (34%), Positives = 20/41 (48%) Query: 151 PNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNY 191 P + PY P +TK HK L+++ E L P+NY Sbjct: 263 PEADAATVPYNPFDLTKVWPHKDYPLIEVGEFELNRNPQNY 303 >4E1J-A nrPDB Length = 520 Score = 26.5 bits (57), Expect = 8.8, Method: Composition-based stats. Identities = 18/43 (41%), Positives = 21/43 (48%), Gaps = 4/43 (9%) Query: 92 PHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDG 134 P +L GT F L LN G+D V RL+T I R DG Sbjct: 279 PGMLKSTYGTGCFAL----LNTGKDMVRSKNRLLTTIAYRLDG 317 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.141 0.437 Lambda K H 0.267 0.0433 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,273,818 Number of Sequences: 36641 Number of extensions: 445739 Number of successful extensions: 910 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 16 Number of HSP's that attempted gapping in prelim test: 896 Number of HSP's gapped (non-prelim): 30 length of query: 227 length of database: 10,336,785 effective HSP length: 94 effective length of query: 133 effective length of database: 6,892,531 effective search space: 916706623 effective search space used: 916706623 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 57 (26.5 bits)