BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O15273 (167 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2F8V-T nrPDB 304 1e-83 3G5B-A nrPDB 33 0.081 2GWH-A nrPDB 30 0.63 1IC8-A nrPDB 28 1.6 5H7C-A nrPDB 27 3.3 2CQS-A nrPDB 27 3.9 >2F8V-T nrPDB Length = 167 Score = 304 bits (778), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 162/167 (97%), Positives = 162/167 (97%) Query: 1 MATSELSCEVSEENCERREAFWAEWKDLTLSTRPEEGCSLHEEDTQRHETYHQQGQCQVL 60 MATSELS EVSEEN ERREAFWAEWKDLTLSTRPEEG SLHEEDTQRHETYHQQGQ QVL Sbjct: 1 MATSELSSEVSEENSERREAFWAEWKDLTLSTRPEEGSSLHEEDTQRHETYHQQGQSQVL 60 Query: 61 VQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALE 120 VQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALE Sbjct: 61 VQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALE 120 Query: 121 TALGGQCVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG 167 TALGGQ VDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG Sbjct: 121 TALGGQSVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG 167 >3G5B-A nrPDB Length = 405 Score = 32.7 bits (73), Expect = 0.081, Method: Compositional matrix adjust. Identities = 27/90 (30%), Positives = 40/90 (44%), Gaps = 5/90 (5%) Query: 45 TQRHETYHQQGQCQVLVQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKE 104 T ET + CQ+ + L+ ++G + Y + L L IF PA + + Sbjct: 111 TLDEETLNTPCYCQLEAKSCHILLDQLGTYVFTGESYSRSAVKRLQLAIFAPALCTSLEY 170 Query: 105 ER-----EDTPIQLQELLALETALGGQCVD 129 EDTP L+E+L LE LGG V+ Sbjct: 171 SLRVYCLEDTPAALKEVLELERTLGGYLVE 200 >2GWH-A nrPDB Length = 298 Score = 29.6 bits (65), Expect = 0.63, Method: Compositional matrix adjust. Identities = 24/83 (28%), Positives = 44/83 (53%), Gaps = 6/83 (7%) Query: 13 ENCERREAFW---AEWKDLTLSTRPEEGCSLHEEDTQ--RHETYHQQGQCQVLVQRSPWL 67 + C+ + W A+ DL +ST P+ G + +E + ++E ++ + QR P+L Sbjct: 26 DTCDIWDKIWNFQAKPDDLLISTYPKAGTTWTQEIVELIQNEGDVEKSKRAPTHQRFPFL 85 Query: 68 MMRMGILGRGL-QEYQLPYQRVL 89 M++ LG GL Q + +P R+L Sbjct: 86 EMKIPSLGSGLEQAHAMPSPRIL 108 >1IC8-A nrPDB Length = 194 Score = 28.5 bits (62), Expect = 1.6, Method: Compositional matrix adjust. Identities = 18/73 (24%), Positives = 37/73 (50%), Gaps = 4/73 (5%) Query: 49 ETYHQQGQCQVLVQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEERED 108 E HQ+ + L+Q PW + +M + LQ++ +P + V+ + + ++ + Sbjct: 12 EAAHQKAVVETLLQEDPWRVAKM--VKSYLQQHNIPQREVVDTTGLNQSHL--SQHLNKG 67 Query: 109 TPIQLQELLALET 121 TP++ Q+ AL T Sbjct: 68 TPMKTQKRAALYT 80 >5H7C-A nrPDB Length = 404 Score = 27.3 bits (59), Expect = 3.3, Method: Compositional matrix adjust. Identities = 25/100 (25%), Positives = 41/100 (41%), Gaps = 12/100 (12%) Query: 80 EYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETALG-GQCV---------- 128 E Q + +L LP + A + +D P Q LLA +L Q V Sbjct: 205 EQQSAFYEILSLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKSLNVNQAVKQLAEKAKEA 264 Query: 129 -DRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG 167 D++EV EI K+L + S L + + +++ A+ Sbjct: 265 TDKEEVIEIVKELAELAKQSTDSELVNEIVKQLAEVAKEA 304 Score = 26.9 bits (58), Expect = 4.7, Method: Compositional matrix adjust. Identities = 25/101 (24%), Positives = 42/101 (41%), Gaps = 12/101 (11%) Query: 79 QEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETALG-GQCV--------- 128 +E Q + +L LP + A + +D P Q LLA +L Q V Sbjct: 8 KEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKSLNVNQAVKQLAEKAKE 67 Query: 129 --DRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG 167 D++EV EI K+L + S L + + +++ A+ Sbjct: 68 ATDKEEVIEIVKELAELAKQSTDSELVNEIVKQLAEVAKEA 108 >2CQS-A nrPDB Length = 842 Score = 26.9 bits (58), Expect = 3.9, Method: Composition-based stats. Identities = 9/32 (28%), Positives = 19/32 (59%), Gaps = 1/32 (3%) Query: 59 VLVQRSPWLMMRMGILGRGLQEYQLPYQRVLP 90 + +PW+++ ++GRG Q + Y+R+ P Sbjct: 683 IFCHNNPWVIIAETVVGRGAQAFDY-YKRITP 713 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2F8V-T nrPDB 277 2e-75 Sequences not found previously or not previously below threshold: 2GWH-A nrPDB 38 0.002 3G5B-A nrPDB 37 0.005 4MKI-B nrPDB 30 0.65 3K5P-A nrPDB 29 0.75 1IC8-A nrPDB 29 1.1 5H7C-A nrPDB 28 1.4 3MDY-A nrPDB 28 1.9 2ZZE-A nrPDB 28 2.0 6EM1-o nrPDB 28 2.4 5H7B-A nrPDB 27 5.0 CONVERGED! >2F8V-T nrPDB Length = 167 Score = 277 bits (708), Expect = 2e-75, Method: Composition-based stats. Identities = 162/167 (97%), Positives = 162/167 (97%) Query: 1 MATSELSCEVSEENCERREAFWAEWKDLTLSTRPEEGCSLHEEDTQRHETYHQQGQCQVL 60 MATSELS EVSEEN ERREAFWAEWKDLTLSTRPEEG SLHEEDTQRHETYHQQGQ QVL Sbjct: 1 MATSELSSEVSEENSERREAFWAEWKDLTLSTRPEEGSSLHEEDTQRHETYHQQGQSQVL 60 Query: 61 VQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALE 120 VQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALE Sbjct: 61 VQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALE 120 Query: 121 TALGGQCVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG 167 TALGGQ VDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG Sbjct: 121 TALGGQSVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG 167 >2GWH-A nrPDB Length = 298 Score = 38.1 bits (87), Expect = 0.002, Method: Composition-based stats. Identities = 25/84 (29%), Positives = 44/84 (52%), Gaps = 7/84 (8%) Query: 21 FWAEWKDLTLSTRPEEGCSLHEEDTQ--RHETYHQQGQCQVLVQRSPWLMMRMGILGRGL 78 F A+ DL +ST P+ G + +E + ++E ++ + QR P+L M++ LG GL Sbjct: 37 FQAKPDDLLISTYPKAGTTWTQEIVELIQNEGDVEKSKRAPTHQRFPFLEMKIPSLGSGL 96 Query: 79 -QEYQLPYQRV----LPLPIFTPA 97 Q + +P R+ LP + P+ Sbjct: 97 EQAHAMPSPRILKTHLPFHLLPPS 120 >3G5B-A nrPDB Length = 405 Score = 36.6 bits (83), Expect = 0.005, Method: Composition-based stats. Identities = 34/118 (28%), Positives = 51/118 (43%), Gaps = 11/118 (9%) Query: 19 EAFWAEWKDLTLSTRPEEGCSLHEED--TQRHETYHQQGQCQVLVQRSPWLMMRMGILGR 76 E +W L T+ +G H E+ T ET + CQ+ + L+ ++G Sbjct: 87 EVIAGDWI-FQLKTQAHQG---HWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVF 142 Query: 77 GLQEYQLPYQRVLPLPIFTPAKMGATKEER-----EDTPIQLQELLALETALGGQCVD 129 + Y + L L IF PA + + EDTP L+E+L LE LGG V+ Sbjct: 143 TGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPAALKEVLELERTLGGYLVE 200 >4MKI-B nrPDB Length = 298 Score = 29.7 bits (65), Expect = 0.65, Method: Composition-based stats. Identities = 21/81 (25%), Positives = 37/81 (45%), Gaps = 10/81 (12%) Query: 80 EYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETAL---------GGQCVDR 130 EYQL + V F P+ +G ++EE ++ + E++ + L GGQ R Sbjct: 97 EYQLFEETVFKDIAFGPSNLGLSEEEVKERVYEAMEIVGISKELADKSPFELSGGQK-RR 155 Query: 131 QEVAEITKQLPPVVPVSKPGA 151 +A I P ++ + +P A Sbjct: 156 VAIAGILAMRPKILILDEPTA 176 >3K5P-A nrPDB Length = 416 Score = 29.3 bits (64), Expect = 0.75, Method: Composition-based stats. Identities = 23/69 (33%), Positives = 33/69 (47%), Gaps = 7/69 (10%) Query: 74 LGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETALGGQCVDRQEV 133 L + LQE L + P+ PA G ER TP+Q E + L +GG + QE Sbjct: 254 LAKVLQEGHLAGAAIDVFPV-EPASNG----ERFSTPLQGLENVILTPHIGGSTEEAQER 308 Query: 134 --AEITKQL 140 E+T++L Sbjct: 309 IGTEVTRKL 317 >1IC8-A nrPDB Length = 194 Score = 28.9 bits (63), Expect = 1.1, Method: Composition-based stats. Identities = 19/79 (24%), Positives = 39/79 (49%), Gaps = 4/79 (5%) Query: 43 EDTQRHETYHQQGQCQVLVQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGAT 102 E+ E HQ+ + L+Q PW + +M + LQ++ +P + V+ + + + Sbjct: 6 ENLSPEEAAHQKAVVETLLQEDPWRVAKM--VKSYLQQHNIPQREVVDTTGLNQSHL--S 61 Query: 103 KEEREDTPIQLQELLALET 121 + + TP++ Q+ AL T Sbjct: 62 QHLNKGTPMKTQKRAALYT 80 >5H7C-A nrPDB Length = 404 Score = 28.5 bits (62), Expect = 1.4, Method: Composition-based stats. Identities = 23/100 (23%), Positives = 40/100 (40%), Gaps = 12/100 (12%) Query: 80 EYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETAL------------GGQC 127 E Q + +L LP + A + +D P Q LLA +L + Sbjct: 205 EQQSAFYEILSLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKSLNVNQAVKQLAEKAKEA 264 Query: 128 VDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG 167 D++EV EI K+L + S L + + +++ A+ Sbjct: 265 TDKEEVIEIVKELAELAKQSTDSELVNEIVKQLAEVAKEA 304 Score = 28.1 bits (61), Expect = 1.9, Method: Composition-based stats. Identities = 23/101 (22%), Positives = 41/101 (40%), Gaps = 12/101 (11%) Query: 79 QEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETAL------------GGQ 126 +E Q + +L LP + A + +D P Q LLA +L + Sbjct: 8 KEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKSLNVNQAVKQLAEKAKE 67 Query: 127 CVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG 167 D++EV EI K+L + S L + + +++ A+ Sbjct: 68 ATDKEEVIEIVKELAELAKQSTDSELVNEIVKQLAEVAKEA 108 >3MDY-A nrPDB Length = 337 Score = 28.1 bits (61), Expect = 1.9, Method: Composition-based stats. Identities = 10/25 (40%), Positives = 16/25 (64%) Query: 66 WLMMRMGILGRGLQEYQLPYQRVLP 90 W + R + G ++EYQLPY ++P Sbjct: 243 WEVARRCVSGGIVEEYQLPYHDLVP 267 >2ZZE-A nrPDB Length = 752 Score = 28.1 bits (61), Expect = 2.0, Method: Composition-based stats. Identities = 26/87 (29%), Positives = 43/87 (49%), Gaps = 7/87 (8%) Query: 67 LMMRMGILGRGLQEY--QLPYQRVLPLPIFTPAKMGATKEEREDT---PIQLQELLALET 121 L++R I R L+E ++P ++ L I K +E ED I+L+E ET Sbjct: 369 LLIRKSI--RHLRELGLEVPLSEIVALHIKELHKTFPEFKEMEDIILEMIELEEKKYAET 426 Query: 122 ALGGQCVDRQEVAEITKQLPPVVPVSK 148 G + R+E+A++ K+ +PV K Sbjct: 427 LRRGSDLVRREIAKLKKKGIKEIPVEK 453 >6EM1-o nrPDB Length = 220 Score = 27.7 bits (60), Expect = 2.4, Method: Composition-based stats. Identities = 14/27 (51%), Positives = 17/27 (62%) Query: 95 TPAKMGATKEEREDTPIQLQELLALET 121 T K TK+ E+ PIQ +E LALET Sbjct: 8 TSTKETVTKQPTEEKPIQEKEELALET 34 >5H7B-A nrPDB Length = 237 Score = 26.6 bits (57), Expect = 5.0, Method: Composition-based stats. Identities = 38/160 (23%), Positives = 57/160 (35%), Gaps = 14/160 (8%) Query: 14 NCERREAFWAEWKDLTLSTRP--EEGCSLHEEDTQRHETYH----QQGQCQVLVQR---S 64 N E+R AF KD + E L+E+ +E H + Q +Q Sbjct: 69 NEEQRNAFIQSLKDDPSQSANLLAEAKKLNEQQAAFYEILHLPNLNEEQRNAFIQSLKDD 128 Query: 65 PWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETALG 124 P + + L E Q + +L LP + A + +D P Q LLA L Sbjct: 129 PSQSANLLAEAKKLNEQQAAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLN 188 Query: 125 GQCVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEA 164 Q E+ + P + + A +SL SQ A Sbjct: 189 EQQAAFYEILHL-----PNLNEEQRNAFIQSLKDDPSQSA 223 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.303 0.133 0.399 Lambda K H 0.267 0.0399 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,037,099 Number of Sequences: 36641 Number of extensions: 220504 Number of successful extensions: 578 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 16 Number of HSP's that attempted gapping in prelim test: 557 Number of HSP's gapped (non-prelim): 35 length of query: 167 length of database: 10,336,785 effective HSP length: 90 effective length of query: 77 effective length of database: 7,039,095 effective search space: 542010315 effective search space used: 542010315 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (20.8 bits) S2: 55 (25.8 bits)