BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O14960 (151 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5B0H-A nrPDB 280 2e-76 1U8V-A nrPDB 27 3.1 5KR3-A nrPDB 27 4.4 4V8M-AE nrPDB 27 4.6 5KQW-A nrPDB 26 6.6 2AHR-A nrPDB 25 8.9 >5B0H-A nrPDB Length = 135 Score = 280 bits (716), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 132/133 (99%), Positives = 133/133 (100%) Query: 19 GPWANICAGKSSNEIRTCDRHGCGQYSAQRSQRPHQGVDILCSAGSTVYAPFTGMIVGQE 78 GPWANICAGKSSNEIRTCDRHGCGQYSAQRSQRPHQGVD+LCSAGSTVYAPFTGMIVGQE Sbjct: 3 GPWANICAGKSSNEIRTCDRHGCGQYSAQRSQRPHQGVDVLCSAGSTVYAPFTGMIVGQE 62 Query: 79 KPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGEKLGTLLPLQKVYPGIQSHVH 138 KPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGEKLGTLLPLQKVYPGIQSHVH Sbjct: 63 KPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGEKLGTLLPLQKVYPGIQSHVH 122 Query: 139 IENCDSSDPTAYL 151 IENCDSSDPTAYL Sbjct: 123 IENCDSSDPTAYL 135 >1U8V-A nrPDB Length = 490 Score = 26.9 bits (58), Expect = 3.1, Method: Compositional matrix adjust. Identities = 15/33 (45%), Positives = 16/33 (48%) Query: 23 NICAGKSSNEIRTCDRHGCGQYSAQRSQRPHQG 55 NIC G S+ RT HG G AQR QG Sbjct: 442 NICLGASAVGYRTESMHGAGSPQAQRIMIARQG 474 >5KR3-A nrPDB Length = 479 Score = 26.6 bits (57), Expect = 4.4, Method: Composition-based stats. Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 6/42 (14%) Query: 64 STVYAPFTGMIVGQ------EKPYQNKNAINNGVRISGRGFC 99 ++ YAP +G+IVG+ EK Q + +G SG C Sbjct: 311 TSAYAPLSGVIVGEKVWDVIEKGSQEHGPMGHGWTYSGHPIC 352 >4V8M-AE nrPDB Length = 174 Score = 26.6 bits (57), Expect = 4.6, Method: Compositional matrix adjust. Identities = 14/54 (25%), Positives = 24/54 (44%), Gaps = 9/54 (16%) Query: 68 APFTGMIVGQEKPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGEKLG 121 P + V EK YQ + A+N +R + + K + G I+ +K+G Sbjct: 11 TPTVDLTVQHEKAYQRQTAVNENIRTASK---------KHVNRSGHIRYSKKIG 55 >5KQW-A nrPDB Length = 479 Score = 26.2 bits (56), Expect = 6.6, Method: Compositional matrix adjust. Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 6/42 (14%) Query: 64 STVYAPFTGMIVGQ------EKPYQNKNAINNGVRISGRGFC 99 ++ YAP +G+IVG+ EK Q + +G SG C Sbjct: 311 TSAYAPLSGVIVGEKVWDVIEKGSQEHGPMGHGWTYSGHPIC 352 >2AHR-A nrPDB Length = 259 Score = 25.4 bits (54), Expect = 8.9, Method: Compositional matrix adjust. Identities = 10/20 (50%), Positives = 12/20 (60%) Query: 49 SQRPHQGVDILCSAGSTVYA 68 SQ PH +D +CS G T A Sbjct: 212 SQSPHDFIDAICSPGGTTIA 231 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5B0H-A nrPDB 282 4e-77 Sequences not found previously or not previously below threshold: 1U8V-A nrPDB 30 0.53 5KR3-A nrPDB 28 2.3 5KQW-A nrPDB 27 2.5 4V8M-AE nrPDB 27 3.0 2AHR-A nrPDB 27 3.0 4M00-A nrPDB 26 7.9 3NYY-A nrPDB 26 8.7 CONVERGED! >5B0H-A nrPDB Length = 135 Score = 282 bits (722), Expect = 4e-77, Method: Composition-based stats. Identities = 132/133 (99%), Positives = 133/133 (100%) Query: 19 GPWANICAGKSSNEIRTCDRHGCGQYSAQRSQRPHQGVDILCSAGSTVYAPFTGMIVGQE 78 GPWANICAGKSSNEIRTCDRHGCGQYSAQRSQRPHQGVD+LCSAGSTVYAPFTGMIVGQE Sbjct: 3 GPWANICAGKSSNEIRTCDRHGCGQYSAQRSQRPHQGVDVLCSAGSTVYAPFTGMIVGQE 62 Query: 79 KPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGEKLGTLLPLQKVYPGIQSHVH 138 KPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGEKLGTLLPLQKVYPGIQSHVH Sbjct: 63 KPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGEKLGTLLPLQKVYPGIQSHVH 122 Query: 139 IENCDSSDPTAYL 151 IENCDSSDPTAYL Sbjct: 123 IENCDSSDPTAYL 135 >1U8V-A nrPDB Length = 490 Score = 29.6 bits (65), Expect = 0.53, Method: Composition-based stats. Identities = 15/33 (45%), Positives = 16/33 (48%) Query: 23 NICAGKSSNEIRTCDRHGCGQYSAQRSQRPHQG 55 NIC G S+ RT HG G AQR QG Sbjct: 442 NICLGASAVGYRTESMHGAGSPQAQRIMIARQG 474 >5KR3-A nrPDB Length = 479 Score = 27.7 bits (60), Expect = 2.3, Method: Composition-based stats. Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 6/42 (14%) Query: 64 STVYAPFTGMIVGQ------EKPYQNKNAINNGVRISGRGFC 99 ++ YAP +G+IVG+ EK Q + +G SG C Sbjct: 311 TSAYAPLSGVIVGEKVWDVIEKGSQEHGPMGHGWTYSGHPIC 352 >5KQW-A nrPDB Length = 479 Score = 27.3 bits (59), Expect = 2.5, Method: Composition-based stats. Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 6/42 (14%) Query: 64 STVYAPFTGMIVGQ------EKPYQNKNAINNGVRISGRGFC 99 ++ YAP +G+IVG+ EK Q + +G SG C Sbjct: 311 TSAYAPLSGVIVGEKVWDVIEKGSQEHGPMGHGWTYSGHPIC 352 >4V8M-AE nrPDB Length = 174 Score = 27.3 bits (59), Expect = 3.0, Method: Composition-based stats. Identities = 14/53 (26%), Positives = 24/53 (45%), Gaps = 9/53 (16%) Query: 69 PFTGMIVGQEKPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGEKLG 121 P + V EK YQ + A+N +R + + K + G I+ +K+G Sbjct: 12 PTVDLTVQHEKAYQRQTAVNENIRTASK---------KHVNRSGHIRYSKKIG 55 >2AHR-A nrPDB Length = 259 Score = 27.3 bits (59), Expect = 3.0, Method: Composition-based stats. Identities = 10/20 (50%), Positives = 12/20 (60%) Query: 49 SQRPHQGVDILCSAGSTVYA 68 SQ PH +D +CS G T A Sbjct: 212 SQSPHDFIDAICSPGGTTIA 231 >4M00-A nrPDB Length = 541 Score = 25.7 bits (55), Expect = 7.9, Method: Composition-based stats. Identities = 15/61 (24%), Positives = 24/61 (39%), Gaps = 9/61 (14%) Query: 59 LCSAGSTVYAPFTGMIVGQEKPYQNKNAINNGVRISGRGFCVKMFYIKPIKYKGPIKKGE 118 + ++G+ Y TG++ + Y K AI G RI K + G + G Sbjct: 60 MTTSGNATYDQSTGIVTLTQDAYSQKGAITLGTRIDSN---------KSFHFSGKVNLGN 110 Query: 119 K 119 K Sbjct: 111 K 111 >3NYY-A nrPDB Length = 252 Score = 25.7 bits (55), Expect = 8.7, Method: Composition-based stats. Identities = 22/81 (27%), Positives = 33/81 (40%), Gaps = 10/81 (12%) Query: 50 QRPHQGVDILCSAGSTVYAPFTGMIVG--QEKPYQNKNAINNGVRISGRGFCVKMFYIKP 107 +R H+G DI+ + Y P M G EK + K G+ + +Y Sbjct: 122 KRGHEGTDIMAEKNTPGYYPVVSMTDGVVTEKGWLEKGGWRIGITAPTGAY---FYYAHL 178 Query: 108 IKY----KG-PIKKGEKLGTL 123 Y KG P+K G+ LG + Sbjct: 179 DSYAELEKGDPVKAGDLLGYM 199 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.141 0.453 Lambda K H 0.267 0.0406 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,725,523 Number of Sequences: 36641 Number of extensions: 284211 Number of successful extensions: 541 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 533 Number of HSP's gapped (non-prelim): 16 length of query: 151 length of database: 10,336,785 effective HSP length: 88 effective length of query: 63 effective length of database: 7,112,377 effective search space: 448079751 effective search space used: 448079751 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 54 (25.4 bits)