BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O14684 (152 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5BQH-A nrPDB 312 3e-86 4BPM-A nrPDB 293 1e-80 5IA9-A nrPDB 99 6e-22 4YOJ-A nrPDB 28 1.9 3NAW-A nrPDB 27 2.5 5GZ5-A nrPDB 27 4.3 >5BQH-A nrPDB Length = 154 Score = 312 bits (800), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 151/152 (99%), Positives = 152/152 (100%) Query: 1 MPAHSLVMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCR 60 +PAHSLVMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCR Sbjct: 3 LPAHSLVMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCR 62 Query: 61 SDPDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGK 120 SDPDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGK Sbjct: 63 SDPDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGK 122 Query: 121 LRAPIRSVTYTLAQLPCASMALQILWEAARHL 152 LRAPIRSVTYTLAQLPCASMALQILWEAARHL Sbjct: 123 LRAPIRSVTYTLAQLPCASMALQILWEAARHL 154 >4BPM-A nrPDB Length = 178 Score = 293 bits (751), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 142/143 (99%), Positives = 142/143 (99%) Query: 10 SPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCRSDPDVERCL 69 SPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCRSDPDVERCL Sbjct: 8 SPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCRSDPDVERCL 67 Query: 70 RAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLRAPIRSVT 129 RAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLRAPIRSVT Sbjct: 68 RAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLRAPIRSVT 127 Query: 130 YTLAQLPCASMALQILWEAARHL 152 YTLAQLPCASMALQILWEAARH Sbjct: 128 YTLAQLPCASMALQILWEAARHF 150 >5IA9-A nrPDB Length = 155 Score = 99.4 bits (246), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 55/143 (38%), Positives = 80/143 (55%), Gaps = 4/143 (2%) Query: 7 VMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHG----GPQYCRSD 62 +M + L AF +T+++ KM ++ T RL K FANPED G ++ R+D Sbjct: 7 LMDNEVLMAFTSYATIILAKMMFLSSATAFQRLTNKVFANPEDCAGFGKGENAKKFLRTD 66 Query: 63 PDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLR 122 VER RAH ND+E I PFL +G +YS GP+ A +HF +F+ R+ HT+AYL L Sbjct: 67 EKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTALIHFRIFVGARIYHTIAYLTPLP 126 Query: 123 APIRSVTYTLAQLPCASMALQIL 145 P R + + + SMA ++L Sbjct: 127 QPNRGLAFFVGYGVTLSMAYRLL 149 >4YOJ-A nrPDB Length = 306 Score = 27.7 bits (60), Expect = 1.9, Method: Compositional matrix adjust. Identities = 11/27 (40%), Positives = 15/27 (55%) Query: 120 KLRAPIRSVTYTLAQLPCASMALQILW 146 K+ AP +V + Q+ C SM L LW Sbjct: 5 KMSAPSGAVENCIVQVTCGSMTLNGLW 31 >3NAW-A nrPDB Length = 613 Score = 27.3 bits (59), Expect = 2.5, Method: Composition-based stats. Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 4/50 (8%) Query: 85 LGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAY-LGKLRA---PIRSVTY 130 L + L PN WM F ++ G V +T Y L L + PI S+ Y Sbjct: 381 LSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPY 430 >5GZ5-A nrPDB Length = 830 Score = 26.6 bits (57), Expect = 4.3, Method: Composition-based stats. Identities = 15/45 (33%), Positives = 18/45 (40%), Gaps = 4/45 (8%) Query: 39 LRKKAFANPEDALRHGGPQYCR----SDPDVERCLRAHRNDMETI 79 L K F NP A P YC PDV C + D+E + Sbjct: 498 LLKNPFYNPSPAKEQSPPLYCLFGPVPSPDVSGCKCSSITDLEAV 542 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5BQH-A nrPDB 254 1e-68 4BPM-A nrPDB 243 2e-65 5IA9-A nrPDB 201 1e-52 Sequences not found previously or not previously below threshold: 6ID1-L nrPDB 27 4.2 3NAW-A nrPDB 27 4.2 1MHS-A nrPDB 26 4.5 3ZUR-A nrPDB 26 7.0 CONVERGED! >5BQH-A nrPDB Length = 154 Score = 254 bits (648), Expect = 1e-68, Method: Composition-based stats. Identities = 151/152 (99%), Positives = 152/152 (100%) Query: 1 MPAHSLVMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCR 60 +PAHSLVMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCR Sbjct: 3 LPAHSLVMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCR 62 Query: 61 SDPDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGK 120 SDPDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGK Sbjct: 63 SDPDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGK 122 Query: 121 LRAPIRSVTYTLAQLPCASMALQILWEAARHL 152 LRAPIRSVTYTLAQLPCASMALQILWEAARHL Sbjct: 123 LRAPIRSVTYTLAQLPCASMALQILWEAARHL 154 >4BPM-A nrPDB Length = 178 Score = 243 bits (621), Expect = 2e-65, Method: Composition-based stats. Identities = 142/143 (99%), Positives = 142/143 (99%) Query: 10 SPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCRSDPDVERCL 69 SPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCRSDPDVERCL Sbjct: 8 SPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCRSDPDVERCL 67 Query: 70 RAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLRAPIRSVT 129 RAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLRAPIRSVT Sbjct: 68 RAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLRAPIRSVT 127 Query: 130 YTLAQLPCASMALQILWEAARHL 152 YTLAQLPCASMALQILWEAARH Sbjct: 128 YTLAQLPCASMALQILWEAARHF 150 >5IA9-A nrPDB Length = 155 Score = 201 bits (511), Expect = 1e-52, Method: Composition-based stats. Identities = 55/143 (38%), Positives = 80/143 (55%), Gaps = 4/143 (2%) Query: 7 VMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHG----GPQYCRSD 62 +M + L AF +T+++ KM ++ T RL K FANPED G ++ R+D Sbjct: 7 LMDNEVLMAFTSYATIILAKMMFLSSATAFQRLTNKVFANPEDCAGFGKGENAKKFLRTD 66 Query: 63 PDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLR 122 VER RAH ND+E I PFL +G +YS GP+ A +HF +F+ R+ HT+AYL L Sbjct: 67 EKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTALIHFRIFVGARIYHTIAYLTPLP 126 Query: 123 APIRSVTYTLAQLPCASMALQIL 145 P R + + + SMA ++L Sbjct: 127 QPNRGLAFFVGYGVTLSMAYRLL 149 >6ID1-L nrPDB Length = 802 Score = 26.9 bits (58), Expect = 4.2, Method: Composition-based stats. Identities = 19/65 (29%), Positives = 26/65 (40%), Gaps = 15/65 (23%) Query: 29 VVAIITGQVRLRKKAFANPEDALR-HGGPQYCRSDPDVERCLRAHR-----------NDM 76 V+ G+ LR K NPED + + P Y + +ER R H ND Sbjct: 434 VINSTPGRTPLRDKLNINPEDGMADYSDPSYVK---QMERESREHLRLGLLGLPAPKNDF 490 Query: 77 ETIYP 81 E + P Sbjct: 491 EIVLP 495 >3NAW-A nrPDB Length = 613 Score = 26.9 bits (58), Expect = 4.2, Method: Composition-based stats. Identities = 11/33 (33%), Positives = 15/33 (45%) Query: 85 LGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAY 117 L + L PN WM F ++ G V +T Y Sbjct: 381 LSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGY 413 >1MHS-A nrPDB Length = 920 Score = 26.5 bits (57), Expect = 4.5, Method: Composition-based stats. Identities = 17/52 (32%), Positives = 24/52 (46%) Query: 47 PEDALRHGGPQYCRSDPDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFV 98 PED L+ S+ V+R + N M+ FL F+ F+GP FV Sbjct: 76 PEDMLQTDTRVGLTSEEVVQRRRKYGLNQMKEEKENHFLKFLGFFVGPIQFV 127 >3ZUR-A nrPDB Length = 960 Score = 26.1 bits (56), Expect = 7.0, Method: Composition-based stats. Identities = 17/65 (26%), Positives = 23/65 (35%), Gaps = 14/65 (21%) Query: 52 RHGGPQYCRSDPDV----ERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFL 107 +G QY R PD E L N P L G + +P V H L++ Sbjct: 182 GYGSTQYIRFSPDFTFGFEESLEVDTN------PLLGAGKFAT----DPAVTLAHQLIYA 231 Query: 108 VGRVA 112 R+ Sbjct: 232 GHRLY 236 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.141 0.424 Lambda K H 0.267 0.0432 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,457,091 Number of Sequences: 36641 Number of extensions: 266364 Number of successful extensions: 688 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 675 Number of HSP's gapped (non-prelim): 18 length of query: 152 length of database: 10,336,785 effective HSP length: 88 effective length of query: 64 effective length of database: 7,112,377 effective search space: 455192128 effective search space used: 455192128 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.4 bits) S2: 55 (25.7 bits)