BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= O14198
         (207 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

5N9J-X nrPDB                                                          436   e-123
3G79-A nrPDB                                                           30   0.74 
1M4W-A nrPDB                                                           30   0.89 
5M9B-A nrPDB                                                           29   1.4  
5IG4-A nrPDB                                                           28   3.3  
4RHE-A nrPDB                                                           27   8.0  

>5N9J-X nrPDB
          Length = 210

 Score =  436 bits (1120), Expect = e-123,   Method: Compositional matrix adjust.
 Identities = 207/207 (100%), Positives = 207/207 (100%)

Query: 1   MQELYLLGVVPSRRFEAVVNSLSKTLDGPKTILEFWVVYRPKDVPPNLPRQPDSWLRLCS 60
           MQELYLLGVVPSRRFEAVVNSLSKTLDGPKTILEFWVVYRPKDVPPNLPRQPDSWLRLCS
Sbjct: 4   MQELYLLGVVPSRRFEAVVNSLSKTLDGPKTILEFWVVYRPKDVPPNLPRQPDSWLRLCS 63

Query: 61  NIESHDETDTEWSKNTQWSMYLEGNSEPKREDKCGIRPVNRAKLTNGSVTEFVEKMGYEF 120
           NIESHDETDTEWSKNTQWSMYLEGNSEPKREDKCGIRPVNRAKLTNGSVTEFVEKMGYEF
Sbjct: 64  NIESHDETDTEWSKNTQWSMYLEGNSEPKREDKCGIRPVNRAKLTNGSVTEFVEKMGYEF 123

Query: 121 SHEYIIQGLEYFFFDTTVRIYQTLIPSQQRSIKPPFHPMNEEQPWILHVYTHVADASNQV 180
           SHEYIIQGLEYFFFDTTVRIYQTLIPSQQRSIKPPFHPMNEEQPWILHVYTHVADASNQV
Sbjct: 124 SHEYIIQGLEYFFFDTTVRIYQTLIPSQQRSIKPPFHPMNEEQPWILHVYTHVADASNQV 183

Query: 181 AMAKAEANLTKVKTLLSAFCDLKNVRL 207
           AMAKAEANLTKVKTLLSAFCDLKNVRL
Sbjct: 184 AMAKAEANLTKVKTLLSAFCDLKNVRL 210


>3G79-A nrPDB
          Length = 478

 Score = 30.0 bits (66), Expect = 0.74,   Method: Compositional matrix adjust.
 Identities = 15/52 (28%), Positives = 24/52 (46%), Gaps = 2/52 (3%)

Query: 17  AVVNSLSKTLDGPKTILEFWVVYRPKDVPPNLPRQP--DSWLRLCSNIESHD 66
           A +  L K +DG K  +  W   +  D   N P +P  D  L+  +++  HD
Sbjct: 342 AALERLGKKMDGSKVAMLGWAFIKDSDDARNTPSEPYRDLCLKAGASVMVHD 393


>1M4W-A nrPDB
          Length = 197

 Score = 29.6 bits (65), Expect = 0.89,   Method: Compositional matrix adjust.
 Identities = 13/44 (29%), Positives = 22/44 (50%), Gaps = 2/44 (4%)

Query: 25  TLDGPKTILEFWVVYRPKDVPPNL--PRQPDSWLRLCSNIESHD 66
           +++G +T  +FW V + K     +      D+W R   N+ SHD
Sbjct: 126 SIEGTRTFQQFWSVRQQKRTSGTITIGNHFDAWARAGMNLGSHD 169


>5M9B-A nrPDB
          Length = 724

 Score = 29.3 bits (64), Expect = 1.4,   Method: Composition-based stats.
 Identities = 20/77 (25%), Positives = 34/77 (44%), Gaps = 4/77 (5%)

Query: 75  NTQWSMYLEGNSEPKREDKCGIRPVNRAKLTNGSVTEFVEKMGYEFSHEYIIQGLEYFFF 134
           N  + +Y  G     +   C +R  +  K    + T   +++G E+SH+ ++ GL YF  
Sbjct: 475 NPDYLLYSRGQGCYGQSTSCYLRGNDGLK----AETSVNKELGIEYSHDGLVAGLTYFRN 530

Query: 135 DTTVRIYQTLIPSQQRS 151
           D   +I   L P    S
Sbjct: 531 DYKNKIESGLSPVDHAS 547


>5IG4-A nrPDB
          Length = 145

 Score = 27.7 bits (60), Expect = 3.3,   Method: Compositional matrix adjust.
 Identities = 18/68 (26%), Positives = 32/68 (47%), Gaps = 6/68 (8%)

Query: 120 FSHEYIIQGLEY--FFFDTTVRIYQTLIPSQQRSIKPPFHPMNEEQPWILHVYTHVADAS 177
           FS+  +++GLE+  F+FD T+   +  +P     + P  H + E+   I   Y  +  + 
Sbjct: 54  FSNGNLVEGLEFHKFYFDNTLS--KRSVPINTTILSPHVHVLGEDAACI--CYMRLTQSV 109

Query: 178 NQVAMAKA 185
           N    AK 
Sbjct: 110 NSSGEAKT 117


>4RHE-A nrPDB
          Length = 209

 Score = 26.6 bits (57), Expect = 8.0,   Method: Compositional matrix adjust.
 Identities = 17/74 (22%), Positives = 32/74 (43%), Gaps = 1/74 (1%)

Query: 43  DVPPNLPRQPDSWLR-LCSNIESHDETDTEWSKNTQWSMYLEGNSEPKREDKCGIRPVNR 101
           +V   +P  PD+  + L    ++ D+  T + K   +S    G+S PK+   C       
Sbjct: 54  EVGVKIPSSPDAASKFLTEKYQAKDQQITVFGKEQWFSPVASGSSAPKQMVVCPCSTGTM 113

Query: 102 AKLTNGSVTEFVEK 115
           A + +G     +E+
Sbjct: 114 AAICHGMSDNLIER 127


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

5N9J-X nrPDB                                                          457   e-129

Sequences not found previously or not previously below threshold:

5DKX-A nrPDB                                                           30   1.0  
5M9B-A nrPDB                                                           29   1.1  
3G79-A nrPDB                                                           28   2.6  
2PXS-A nrPDB                                                           28   3.3  
5IG4-A nrPDB                                                           28   3.6  
1M4W-A nrPDB                                                           28   3.7  


CONVERGED!
>5N9J-X nrPDB
          Length = 210

 Score =  457 bits (1176), Expect = e-129,   Method: Composition-based stats.
 Identities = 207/207 (100%), Positives = 207/207 (100%)

Query: 1   MQELYLLGVVPSRRFEAVVNSLSKTLDGPKTILEFWVVYRPKDVPPNLPRQPDSWLRLCS 60
           MQELYLLGVVPSRRFEAVVNSLSKTLDGPKTILEFWVVYRPKDVPPNLPRQPDSWLRLCS
Sbjct: 4   MQELYLLGVVPSRRFEAVVNSLSKTLDGPKTILEFWVVYRPKDVPPNLPRQPDSWLRLCS 63

Query: 61  NIESHDETDTEWSKNTQWSMYLEGNSEPKREDKCGIRPVNRAKLTNGSVTEFVEKMGYEF 120
           NIESHDETDTEWSKNTQWSMYLEGNSEPKREDKCGIRPVNRAKLTNGSVTEFVEKMGYEF
Sbjct: 64  NIESHDETDTEWSKNTQWSMYLEGNSEPKREDKCGIRPVNRAKLTNGSVTEFVEKMGYEF 123

Query: 121 SHEYIIQGLEYFFFDTTVRIYQTLIPSQQRSIKPPFHPMNEEQPWILHVYTHVADASNQV 180
           SHEYIIQGLEYFFFDTTVRIYQTLIPSQQRSIKPPFHPMNEEQPWILHVYTHVADASNQV
Sbjct: 124 SHEYIIQGLEYFFFDTTVRIYQTLIPSQQRSIKPPFHPMNEEQPWILHVYTHVADASNQV 183

Query: 181 AMAKAEANLTKVKTLLSAFCDLKNVRL 207
           AMAKAEANLTKVKTLLSAFCDLKNVRL
Sbjct: 184 AMAKAEANLTKVKTLLSAFCDLKNVRL 210


>5DKX-A nrPDB
          Length = 951

 Score = 29.7 bits (65), Expect = 1.0,   Method: Composition-based stats.
 Identities = 25/92 (27%), Positives = 36/92 (39%), Gaps = 21/92 (22%)

Query: 11  PSRRFEAVVN----SLSKTLDGPKTI---------LEFWVVYRPKDVPPNLPRQPDSWLR 57
           PS +FEA +     S+    DG   I         +E W   RPK  PP  P + +   +
Sbjct: 143 PSSKFEATIKFAPFSIDFKRDGASHIKFNDQGLLNIEHW---RPKIDPPPEPEKKEGEQQ 199

Query: 58  LCSNIESHDETDTEWSKNTQWSMYLEGNSEPK 89
                E+  E D+ W     W     GN++ K
Sbjct: 200 PDKKEEAPREDDSTW-----WEESFGGNTDSK 226


>5M9B-A nrPDB
          Length = 724

 Score = 29.3 bits (64), Expect = 1.1,   Method: Composition-based stats.
 Identities = 20/77 (25%), Positives = 34/77 (44%), Gaps = 4/77 (5%)

Query: 75  NTQWSMYLEGNSEPKREDKCGIRPVNRAKLTNGSVTEFVEKMGYEFSHEYIIQGLEYFFF 134
           N  + +Y  G     +   C +R  +  K    + T   +++G E+SH+ ++ GL YF  
Sbjct: 475 NPDYLLYSRGQGCYGQSTSCYLRGNDGLK----AETSVNKELGIEYSHDGLVAGLTYFRN 530

Query: 135 DTTVRIYQTLIPSQQRS 151
           D   +I   L P    S
Sbjct: 531 DYKNKIESGLSPVDHAS 547


>3G79-A nrPDB
          Length = 478

 Score = 28.1 bits (61), Expect = 2.6,   Method: Composition-based stats.
 Identities = 15/52 (28%), Positives = 24/52 (46%), Gaps = 2/52 (3%)

Query: 17  AVVNSLSKTLDGPKTILEFWVVYRPKDVPPNLPRQP--DSWLRLCSNIESHD 66
           A +  L K +DG K  +  W   +  D   N P +P  D  L+  +++  HD
Sbjct: 342 AALERLGKKMDGSKVAMLGWAFIKDSDDARNTPSEPYRDLCLKAGASVMVHD 393


>2PXS-A nrPDB
          Length = 228

 Score = 27.7 bits (60), Expect = 3.3,   Method: Composition-based stats.
 Identities = 15/54 (27%), Positives = 25/54 (46%), Gaps = 7/54 (12%)

Query: 27  DGPKTILEFWVVYRPKDVPPNLPRQPDSWLRLCSNIESHDETDTEWSKNTQWSM 80
           DG +   +F  VY+ K VP  +P     W  +   +   D +D   +KN +W +
Sbjct: 170 DGGRLRCQFDTVYKAKSVPRKMP----DWHFIQHKLTREDRSD---AKNQKWHL 216


>5IG4-A nrPDB
          Length = 145

 Score = 27.7 bits (60), Expect = 3.6,   Method: Composition-based stats.
 Identities = 23/85 (27%), Positives = 39/85 (45%), Gaps = 11/85 (12%)

Query: 102 AKLTNGSVTEFVEKMGYEFSHEYIIQGLEY--FFFDTTVRIYQTLIPSQQRSIKPPFHPM 159
           +KL +  VT F       FS+  +++GLE+  F+FD T+   +  +P     + P  H +
Sbjct: 41  SKLVDPHVTCF-----EPFSNGNLVEGLEFHKFYFDNTLS--KRSVPINTTILSPHVHVL 93

Query: 160 NEEQPWILHVYTHVADASNQVAMAK 184
            E+   I   Y  +  + N    AK
Sbjct: 94  GEDAACI--CYMRLTQSVNSSGEAK 116


>1M4W-A nrPDB
          Length = 197

 Score = 27.7 bits (60), Expect = 3.7,   Method: Composition-based stats.
 Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 2/46 (4%)

Query: 23  SKTLDGPKTILEFWVVYRPKDVPPNLP--RQPDSWLRLCSNIESHD 66
           + +++G +T  +FW V + K     +      D+W R   N+ SHD
Sbjct: 124 APSIEGTRTFQQFWSVRQQKRTSGTITIGNHFDAWARAGMNLGSHD 169


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.318    0.133    0.409 

Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,850,975
Number of Sequences: 36641
Number of extensions: 366047
Number of successful extensions: 806
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 802
Number of HSP's gapped (non-prelim): 16
length of query: 207
length of database: 10,336,785
effective HSP length: 93
effective length of query: 114
effective length of database: 6,929,172
effective search space: 789925608
effective search space used: 789925608
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)