BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O14010 (136 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5N9J-Z nrPDB 276 2e-75 4IQB-A nrPDB 27 3.1 1CT9-A nrPDB 26 4.9 3U0R-A nrPDB 26 6.0 3JB9-j nrPDB 25 7.3 1RK6-A nrPDB 25 7.5 4K1P-A nrPDB 25 8.9 >5N9J-Z nrPDB Length = 136 Score = 276 bits (706), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 136/136 (100%), Positives = 136/136 (100%) Query: 1 MSSDSFQRQLVQRTNTLNSSIDNATLTILSRFQDILDIAINEGKDKYTVAPEVYQIECHT 60 MSSDSFQRQLVQRTNTLNSSIDNATLTILSRFQDILDIAINEGKDKYTVAPEVYQIECHT Sbjct: 1 MSSDSFQRQLVQRTNTLNSSIDNATLTILSRFQDILDIAINEGKDKYTVAPEVYQIECHT 60 Query: 61 VSMVRAVEQLLDVSRQIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLEVSLI 120 VSMVRAVEQLLDVSRQIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLEVSLI Sbjct: 61 VSMVRAVEQLLDVSRQIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLEVSLI 120 Query: 121 EPEASETTEAPTVSDT 136 EPEASETTEAPTVSDT Sbjct: 121 EPEASETTEAPTVSDT 136 >4IQB-A nrPDB Length = 315 Score = 26.6 bits (57), Expect = 3.1, Method: Compositional matrix adjust. Identities = 11/23 (47%), Positives = 16/23 (69%) Query: 56 IECHTVSMVRAVEQLLDVSRQIK 78 ++CHT + V+QL +V RQIK Sbjct: 149 VDCHTDYSGQGVDQLAEVIRQIK 171 >1CT9-A nrPDB Length = 553 Score = 26.2 bits (56), Expect = 4.9, Method: Composition-based stats. Identities = 15/52 (28%), Positives = 27/52 (51%), Gaps = 2/52 (3%) Query: 36 LDIAINEGKDKYTVAPEVYQIECHTVSMVRAVEQLLDVSRQIKSYWLTNSLS 87 + + EG D + +Y IE + V+ +RA + +SR+IK+ + LS Sbjct: 297 IHFTVQEGLD--AIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLS 346 >3U0R-A nrPDB Length = 507 Score = 25.8 bits (55), Expect = 6.0, Method: Compositional matrix adjust. Identities = 13/31 (41%), Positives = 19/31 (61%) Query: 99 DLEKVKRTLTKLQNHLLEVSLIEPEASETTE 129 D+EK++ L KL + LLE + PE +E E Sbjct: 302 DMEKLETNLRKLFDKLLEYMPLPPEEAENGE 332 >3JB9-j nrPDB Length = 239 Score = 25.4 bits (54), Expect = 7.3, Method: Compositional matrix adjust. Identities = 14/31 (45%), Positives = 21/31 (67%), Gaps = 3/31 (9%) Query: 98 PDLEKVK---RTLTKLQNHLLEVSLIEPEAS 125 PD+ KV +TL+ QNHL E++ ++P AS Sbjct: 80 PDIGKVLPNLKTLSLAQNHLQEIADLDPLAS 110 >1RK6-A nrPDB Length = 496 Score = 25.4 bits (54), Expect = 7.5, Method: Composition-based stats. Identities = 10/21 (47%), Positives = 14/21 (66%) Query: 33 QDILDIAINEGKDKYTVAPEV 53 +D+ +IA GK KY V PE+ Sbjct: 330 RDLEEIAAERGKSKYDVVPEL 350 >4K1P-A nrPDB Length = 360 Score = 25.4 bits (54), Expect = 8.9, Method: Compositional matrix adjust. Identities = 12/29 (41%), Positives = 19/29 (65%) Query: 27 TILSRFQDILDIAINEGKDKYTVAPEVYQ 55 TIL+R Q+I+ +IN GK +T+ + Q Sbjct: 193 TILNRPQEIIKGSINIGKQVFTITNQTAQ 221 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5N9J-Z nrPDB 252 5e-68 Sequences not found previously or not previously below threshold: 3JB9-j nrPDB 28 1.3 3U0R-A nrPDB 28 1.4 5APN-x nrPDB 27 2.2 4YDO-B nrPDB 27 2.3 1CT9-A nrPDB 27 2.3 6ERF-B nrPDB 27 2.4 4IQB-A nrPDB 27 3.0 2A2J-A nrPDB 26 5.0 5EJB-B nrPDB 26 6.1 4K1P-A nrPDB 26 7.3 CONVERGED! >5N9J-Z nrPDB Length = 136 Score = 252 bits (642), Expect = 5e-68, Method: Composition-based stats. Identities = 136/136 (100%), Positives = 136/136 (100%) Query: 1 MSSDSFQRQLVQRTNTLNSSIDNATLTILSRFQDILDIAINEGKDKYTVAPEVYQIECHT 60 MSSDSFQRQLVQRTNTLNSSIDNATLTILSRFQDILDIAINEGKDKYTVAPEVYQIECHT Sbjct: 1 MSSDSFQRQLVQRTNTLNSSIDNATLTILSRFQDILDIAINEGKDKYTVAPEVYQIECHT 60 Query: 61 VSMVRAVEQLLDVSRQIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLEVSLI 120 VSMVRAVEQLLDVSRQIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLEVSLI Sbjct: 61 VSMVRAVEQLLDVSRQIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLEVSLI 120 Query: 121 EPEASETTEAPTVSDT 136 EPEASETTEAPTVSDT Sbjct: 121 EPEASETTEAPTVSDT 136 >3JB9-j nrPDB Length = 239 Score = 27.8 bits (60), Expect = 1.3, Method: Composition-based stats. Identities = 14/31 (45%), Positives = 21/31 (67%), Gaps = 3/31 (9%) Query: 98 PDLEKVK---RTLTKLQNHLLEVSLIEPEAS 125 PD+ KV +TL+ QNHL E++ ++P AS Sbjct: 80 PDIGKVLPNLKTLSLAQNHLQEIADLDPLAS 110 >3U0R-A nrPDB Length = 507 Score = 27.8 bits (60), Expect = 1.4, Method: Composition-based stats. Identities = 13/31 (41%), Positives = 19/31 (61%) Query: 99 DLEKVKRTLTKLQNHLLEVSLIEPEASETTE 129 D+EK++ L KL + LLE + PE +E E Sbjct: 302 DMEKLETNLRKLFDKLLEYMPLPPEEAENGE 332 >5APN-x nrPDB Length = 616 Score = 27.0 bits (58), Expect = 2.2, Method: Composition-based stats. Identities = 14/62 (22%), Positives = 33/62 (53%) Query: 57 ECHTVSMVRAVEQLLDVSRQIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLE 116 + H + + + + L + +Q + + LS++FP V +E +L+ +K+ L + + E Sbjct: 389 QTHILKLKTSRQLLTKIDKQGVYPFKLSHLSSNFPFVHENEEELQSLKKDLKSFRLGMSE 448 Query: 117 VS 118 +S Sbjct: 449 IS 450 >4YDO-B nrPDB Length = 587 Score = 27.0 bits (58), Expect = 2.3, Method: Composition-based stats. Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Query: 69 QLLDVSRQIKSYWLTNSLS-TSFPTVDYSEPDLEKVKRTLTKLQNH 113 Q+LDV+ YWL NS PT++ ++ L+ + +TK N+ Sbjct: 188 QVLDVNHSWMIYWLLNSYYLIQNPTMEINQSILDLIVNKITKCINY 233 >1CT9-A nrPDB Length = 553 Score = 27.0 bits (58), Expect = 2.3, Method: Composition-based stats. Identities = 15/52 (28%), Positives = 27/52 (51%), Gaps = 2/52 (3%) Query: 36 LDIAINEGKDKYTVAPEVYQIECHTVSMVRAVEQLLDVSRQIKSYWLTNSLS 87 + + EG D + +Y IE + V+ +RA + +SR+IK+ + LS Sbjct: 297 IHFTVQEGLD--AIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLS 346 >6ERF-B nrPDB Length = 572 Score = 27.0 bits (58), Expect = 2.4, Method: Composition-based stats. Identities = 19/82 (23%), Positives = 36/82 (43%), Gaps = 13/82 (15%) Query: 60 TVSMVRAVEQLLDVSRQIKSYWLTNSLSTSFPTVDYSEPDLEKV-----------KRTLT 108 T + + AV+ L+D K T++L FPT P +++ + L Sbjct: 464 TEAQLNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLP 523 Query: 109 KLQNHLLEVSLIEPEASETTEA 130 +Q H+ +++ P A TT++ Sbjct: 524 PIQQHIW--NMLNPPAEVTTKS 543 >4IQB-A nrPDB Length = 315 Score = 26.7 bits (57), Expect = 3.0, Method: Composition-based stats. Identities = 11/23 (47%), Positives = 16/23 (69%) Query: 56 IECHTVSMVRAVEQLLDVSRQIK 78 ++CHT + V+QL +V RQIK Sbjct: 149 VDCHTDYSGQGVDQLAEVIRQIK 171 >2A2J-A nrPDB Length = 246 Score = 25.9 bits (55), Expect = 5.0, Method: Composition-based stats. Identities = 11/37 (29%), Positives = 18/37 (48%) Query: 19 SSIDNATLTILSRFQDILDIAINEGKDKYTVAPEVYQ 55 + +DN + RF D I + G Y +APE+ + Sbjct: 183 AQLDNQLAEVTRRFADQDQIPVPPGWGGYRIAPEIVE 219 >5EJB-B nrPDB Length = 501 Score = 25.9 bits (55), Expect = 6.1, Method: Composition-based stats. Identities = 24/109 (22%), Positives = 46/109 (42%), Gaps = 13/109 (11%) Query: 21 IDNATLTILSRFQDILDI-----AINEGKDKYTVAPEVYQIECHTVSMVRAVEQLLDVSR 75 IDN T T + I+ + +IN + V P VY + S + ++ Q L + Sbjct: 387 IDNTTCTTVVLGNIIISLGKYLGSINYNSESIAVGPPVYTDKVDISSQISSMNQSL---Q 443 Query: 76 QIKSYWLTNSLSTSFPTVDYSEPDLEKVKRTLTKLQNHLLEVSLIEPEA 124 Q K Y + + +D E +E++ + ++N + + + EA Sbjct: 444 QSKDY-----IKEAQKILDNVEDKIEEILSKIYHIENEIARIKKLIGEA 487 >4K1P-A nrPDB Length = 360 Score = 25.5 bits (54), Expect = 7.3, Method: Composition-based stats. Identities = 14/49 (28%), Positives = 25/49 (51%) Query: 9 QLVQRTNTLNSSIDNATLTILSRFQDILDIAINEGKDKYTVAPEVYQIE 57 +L + + I TIL+R Q+I+ +IN GK +T+ + Q + Sbjct: 175 KLREDIKRIQGEIQAELTTILNRPQEIIKGSINIGKQVFTITNQTAQTK 223 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.311 0.125 0.335 Lambda K H 0.267 0.0387 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,407,738 Number of Sequences: 36641 Number of extensions: 141567 Number of successful extensions: 318 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 305 Number of HSP's gapped (non-prelim): 23 length of query: 136 length of database: 10,336,785 effective HSP length: 87 effective length of query: 49 effective length of database: 7,149,018 effective search space: 350301882 effective search space used: 350301882 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 53 (25.1 bits)