BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O08604 (253 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4G59-A nrPDB 431 e-121 6AVF-H nrPDB 30 0.77 1BII-A nrPDB 30 1.4 1T7V-A nrPDB 29 1.5 1JUG-A nrPDB 28 4.1 >4G59-A nrPDB Length = 205 Score = 431 bits (1107), Expect = e-121, Method: Compositional matrix adjust. Identities = 204/204 (100%), Positives = 204/204 (100%) Query: 29 LDDAHSLRCNLTIKAPTPADPLWYEAKCLVDEILILHLSNINKTMTSGDPGETANATEVG 88 LDDAHSLRCNLTIKAPTPADPLWYEAKCLVDEILILHLSNINKTMTSGDPGETANATEVG Sbjct: 2 LDDAHSLRCNLTIKAPTPADPLWYEAKCLVDEILILHLSNINKTMTSGDPGETANATEVG 61 Query: 89 ECLTQPVNDLCQKLRDKVSNTKVDTHKTNGYPHLQVTMIYPQSQGQTPSATWEFNISDSY 148 ECLTQPVNDLCQKLRDKVSNTKVDTHKTNGYPHLQVTMIYPQSQGQTPSATWEFNISDSY Sbjct: 62 ECLTQPVNDLCQKLRDKVSNTKVDTHKTNGYPHLQVTMIYPQSQGQTPSATWEFNISDSY 121 Query: 149 FFTFYTENMSWRSANDESGVIMNKWNDDGDLVQRLKYFIPECRQKIDEFLKQSKEKPRST 208 FFTFYTENMSWRSANDESGVIMNKWNDDGDLVQRLKYFIPECRQKIDEFLKQSKEKPRST Sbjct: 122 FFTFYTENMSWRSANDESGVIMNKWNDDGDLVQRLKYFIPECRQKIDEFLKQSKEKPRST 181 Query: 209 SRSPSITQLTSTSPLPPPSHSTSK 232 SRSPSITQLTSTSPLPPPSHSTSK Sbjct: 182 SRSPSITQLTSTSPLPPPSHSTSK 205 >6AVF-H nrPDB Length = 362 Score = 30.4 bits (67), Expect = 0.77, Method: Compositional matrix adjust. Identities = 13/47 (27%), Positives = 23/47 (48%) Query: 158 SWRSANDESGVIMNKWNDDGDLVQRLKYFIPECRQKIDEFLKQSKEK 204 SW +A+ + + KW + QR Y EC + + +L+ K+K Sbjct: 156 SWTAADTAAQITQRKWEAAREAEQRRAYLEGECVEWLRRYLENGKDK 202 >1BII-A nrPDB Length = 365 Score = 29.6 bits (65), Expect = 1.4, Method: Compositional matrix adjust. Identities = 14/70 (20%), Positives = 28/70 (40%) Query: 130 QSQGQTPSATWEFNISDSYFFTFYTENMSWRSANDESGVIMNKWNDDGDLVQRLKYFIPE 189 +S G+ W+F + + +W +A+ + + KW G + Y E Sbjct: 128 ESDGRLLRGYWQFAYDGCDYIALNEDLKTWTAADMAAQITRRKWEQAGAAERDRAYLEGE 187 Query: 190 CRQKIDEFLK 199 C + + +LK Sbjct: 188 CVEWLRRYLK 197 >1T7V-A nrPDB Length = 278 Score = 29.3 bits (64), Expect = 1.5, Method: Compositional matrix adjust. Identities = 18/70 (25%), Positives = 31/70 (44%), Gaps = 1/70 (1%) Query: 134 QTPSATWEFNISDSYFFTFYTENMSWRSANDESGVIMNKWNDDGDLVQRLK-YFIPECRQ 192 ++ A W++ + F E +W + + + KW + VQR K Y EC Sbjct: 109 RSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPA 168 Query: 193 KIDEFLKQSK 202 + ++LK SK Sbjct: 169 TLRKYLKYSK 178 >1JUG-A nrPDB Length = 125 Score = 28.1 bits (61), Expect = 4.1, Method: Compositional matrix adjust. Identities = 18/55 (32%), Positives = 25/55 (45%), Gaps = 3/55 (5%) Query: 96 NDLCQKLRDKVSNTKVDTHKTNG---YPHLQVTMIYPQSQGQTPSATWEFNISDS 147 N +C + NT+ H T+G Y LQ+ Y G+TP + NIS S Sbjct: 27 NWVCTAFHESSYNTRATNHNTDGSTDYGILQINSRYWCHDGKTPGSKNACNISCS 81 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4G59-A nrPDB 450 e-127 Sequences not found previously or not previously below threshold: 1T7V-A nrPDB 32 0.30 6AVF-H nrPDB 30 0.71 1JUG-A nrPDB 29 2.1 1BII-A nrPDB 29 2.8 5ET1-A nrPDB 27 5.8 CONVERGED! >4G59-A nrPDB Length = 205 Score = 450 bits (1157), Expect = e-127, Method: Composition-based stats. Identities = 204/204 (100%), Positives = 204/204 (100%) Query: 29 LDDAHSLRCNLTIKAPTPADPLWYEAKCLVDEILILHLSNINKTMTSGDPGETANATEVG 88 LDDAHSLRCNLTIKAPTPADPLWYEAKCLVDEILILHLSNINKTMTSGDPGETANATEVG Sbjct: 2 LDDAHSLRCNLTIKAPTPADPLWYEAKCLVDEILILHLSNINKTMTSGDPGETANATEVG 61 Query: 89 ECLTQPVNDLCQKLRDKVSNTKVDTHKTNGYPHLQVTMIYPQSQGQTPSATWEFNISDSY 148 ECLTQPVNDLCQKLRDKVSNTKVDTHKTNGYPHLQVTMIYPQSQGQTPSATWEFNISDSY Sbjct: 62 ECLTQPVNDLCQKLRDKVSNTKVDTHKTNGYPHLQVTMIYPQSQGQTPSATWEFNISDSY 121 Query: 149 FFTFYTENMSWRSANDESGVIMNKWNDDGDLVQRLKYFIPECRQKIDEFLKQSKEKPRST 208 FFTFYTENMSWRSANDESGVIMNKWNDDGDLVQRLKYFIPECRQKIDEFLKQSKEKPRST Sbjct: 122 FFTFYTENMSWRSANDESGVIMNKWNDDGDLVQRLKYFIPECRQKIDEFLKQSKEKPRST 181 Query: 209 SRSPSITQLTSTSPLPPPSHSTSK 232 SRSPSITQLTSTSPLPPPSHSTSK Sbjct: 182 SRSPSITQLTSTSPLPPPSHSTSK 205 >1T7V-A nrPDB Length = 278 Score = 31.6 bits (70), Expect = 0.30, Method: Composition-based stats. Identities = 18/70 (25%), Positives = 31/70 (44%), Gaps = 1/70 (1%) Query: 134 QTPSATWEFNISDSYFFTFYTENMSWRSANDESGVIMNKWNDDGDLVQRLK-YFIPECRQ 192 ++ A W++ + F E +W + + + KW + VQR K Y EC Sbjct: 109 RSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPA 168 Query: 193 KIDEFLKQSK 202 + ++LK SK Sbjct: 169 TLRKYLKYSK 178 >6AVF-H nrPDB Length = 362 Score = 30.4 bits (67), Expect = 0.71, Method: Composition-based stats. Identities = 13/47 (27%), Positives = 23/47 (48%) Query: 158 SWRSANDESGVIMNKWNDDGDLVQRLKYFIPECRQKIDEFLKQSKEK 204 SW +A+ + + KW + QR Y EC + + +L+ K+K Sbjct: 156 SWTAADTAAQITQRKWEAAREAEQRRAYLEGECVEWLRRYLENGKDK 202 >1JUG-A nrPDB Length = 125 Score = 28.9 bits (63), Expect = 2.1, Method: Composition-based stats. Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 4/60 (6%) Query: 91 LTQPVNDLCQKLRDKVSNTKVDTHKTNG---YPHLQVTMIYPQSQGQTPSATWEFNISDS 147 +T P N +C + NT+ H T+G Y LQ+ Y G+TP + NIS S Sbjct: 23 ITLP-NWVCTAFHESSYNTRATNHNTDGSTDYGILQINSRYWCHDGKTPGSKNACNISCS 81 >1BII-A nrPDB Length = 365 Score = 28.5 bits (62), Expect = 2.8, Method: Composition-based stats. Identities = 14/70 (20%), Positives = 28/70 (40%) Query: 130 QSQGQTPSATWEFNISDSYFFTFYTENMSWRSANDESGVIMNKWNDDGDLVQRLKYFIPE 189 +S G+ W+F + + +W +A+ + + KW G + Y E Sbjct: 128 ESDGRLLRGYWQFAYDGCDYIALNEDLKTWTAADMAAQITRRKWEQAGAAERDRAYLEGE 187 Query: 190 CRQKIDEFLK 199 C + + +LK Sbjct: 188 CVEWLRRYLK 197 >5ET1-A nrPDB Length = 353 Score = 27.4 bits (59), Expect = 5.8, Method: Composition-based stats. Identities = 20/63 (31%), Positives = 29/63 (46%), Gaps = 5/63 (7%) Query: 62 LILHLSNINKTMTSGDPGETANATEVGE---CLTQPVNDLCQKLRDKVSNTKVDTHKTNG 118 L+LH + I++ + G P +A E GE C VN +RD T D + NG Sbjct: 260 LLLHGAEISQDLWGGTP--LHDAAENGELECCQILAVNGAGLDVRDHDGYTAADLAEFNG 317 Query: 119 YPH 121 + H Sbjct: 318 HTH 320 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.132 0.398 Lambda K H 0.267 0.0402 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,787,844 Number of Sequences: 36641 Number of extensions: 444709 Number of successful extensions: 1094 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 1086 Number of HSP's gapped (non-prelim): 15 length of query: 253 length of database: 10,336,785 effective HSP length: 95 effective length of query: 158 effective length of database: 6,855,890 effective search space: 1083230620 effective search space used: 1083230620 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 58 (27.0 bits)