BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= O02372 (153 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2GTE-A nrPDB 263 2e-71 3Q8I-A nrPDB 114 2e-26 2CXI-A nrPDB 30 0.58 4V3P-LV nrPDB 28 1.2 5MRE-H nrPDB 26 5.3 >2GTE-A nrPDB Length = 124 Score = 263 bits (672), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 124/124 (100%), Positives = 124/124 (100%) Query: 30 MTMEQFLTSLDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNK 89 MTMEQFLTSLDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNK Sbjct: 1 MTMEQFLTSLDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNK 60 Query: 90 KGEFNAPKALAQLPHLVPPEMMEMSRKSVEACRDTHKQFKESCERVYQTAKCFSENADGQ 149 KGEFNAPKALAQLPHLVPPEMMEMSRKSVEACRDTHKQFKESCERVYQTAKCFSENADGQ Sbjct: 61 KGEFNAPKALAQLPHLVPPEMMEMSRKSVEACRDTHKQFKESCERVYQTAKCFSENADGQ 120 Query: 150 FMWP 153 FMWP Sbjct: 121 FMWP 124 >3Q8I-A nrPDB Length = 124 Score = 114 bits (285), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 51/124 (41%), Positives = 81/124 (65%) Query: 30 MTMEQFLTSLDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNK 89 MTM+Q S+DM+R CAPKFK++ +L LR F P ++L CY C++ MAGT+ K Sbjct: 1 MTMKQLTNSMDMMRQACAPKFKVEEAELHGLRKSIFPANPDKELKCYAMCIAQMAGTMTK 60 Query: 90 KGEFNAPKALAQLPHLVPPEMMEMSRKSVEACRDTHKQFKESCERVYQTAKCFSENADGQ 149 KGE + K +AQ+ ++PPEM M+++++ C+DT +K+ C++ Y +AKC ++ Sbjct: 61 KGEISFSKTMAQIEAMLPPEMKTMAKEALTHCKDTQTSYKDPCDKAYFSAKCAADFTPDT 120 Query: 150 FMWP 153 FM+P Sbjct: 121 FMFP 124 >2CXI-A nrPDB Length = 348 Score = 29.6 bits (65), Expect = 0.58, Method: Compositional matrix adjust. Identities = 13/32 (40%), Positives = 18/32 (56%) Query: 48 PKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKC 79 PKF + DL+RL F+ +DL+ Y KC Sbjct: 2 PKFDVSKSDLERLIGRSFSIEEWEDLVLYAKC 33 >4V3P-LV nrPDB Length = 171 Score = 28.5 bits (62), Expect = 1.2, Method: Compositional matrix adjust. Identities = 13/33 (39%), Positives = 19/33 (57%), Gaps = 3/33 (9%) Query: 124 THKQ---FKESCERVYQTAKCFSENADGQFMWP 153 HKQ F+ C V +TA+ S +++GQ WP Sbjct: 53 AHKQAIPFRRYCGGVGRTAQAKSRHSNGQGRWP 85 >5MRE-H nrPDB Length = 160 Score = 26.2 bits (56), Expect = 5.3, Method: Compositional matrix adjust. Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%) Query: 44 SGCAPKFKLKTEDLDRLRVGDFNFPP-SQDLMCY 76 SG PK KL+ + LDRL+V D + P Q++ + Sbjct: 109 SGMLPKNKLRKQRLDRLKVFDGSENPYKQNITAF 142 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2GTE-A nrPDB 213 4e-56 3Q8I-A nrPDB 194 2e-50 Sequences not found previously or not previously below threshold: 2CXI-A nrPDB 29 0.67 4I9E-A nrPDB 29 1.1 5BJS-A nrPDB 28 1.7 1C5G-A nrPDB 28 1.9 1INP-A nrPDB 26 6.8 3LW2-A nrPDB 25 9.6 CONVERGED! >2GTE-A nrPDB Length = 124 Score = 213 bits (541), Expect = 4e-56, Method: Composition-based stats. Identities = 124/124 (100%), Positives = 124/124 (100%) Query: 30 MTMEQFLTSLDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNK 89 MTMEQFLTSLDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNK Sbjct: 1 MTMEQFLTSLDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNK 60 Query: 90 KGEFNAPKALAQLPHLVPPEMMEMSRKSVEACRDTHKQFKESCERVYQTAKCFSENADGQ 149 KGEFNAPKALAQLPHLVPPEMMEMSRKSVEACRDTHKQFKESCERVYQTAKCFSENADGQ Sbjct: 61 KGEFNAPKALAQLPHLVPPEMMEMSRKSVEACRDTHKQFKESCERVYQTAKCFSENADGQ 120 Query: 150 FMWP 153 FMWP Sbjct: 121 FMWP 124 >3Q8I-A nrPDB Length = 124 Score = 194 bits (492), Expect = 2e-50, Method: Composition-based stats. Identities = 51/124 (41%), Positives = 81/124 (65%) Query: 30 MTMEQFLTSLDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNK 89 MTM+Q S+DM+R CAPKFK++ +L LR F P ++L CY C++ MAGT+ K Sbjct: 1 MTMKQLTNSMDMMRQACAPKFKVEEAELHGLRKSIFPANPDKELKCYAMCIAQMAGTMTK 60 Query: 90 KGEFNAPKALAQLPHLVPPEMMEMSRKSVEACRDTHKQFKESCERVYQTAKCFSENADGQ 149 KGE + K +AQ+ ++PPEM M+++++ C+DT +K+ C++ Y +AKC ++ Sbjct: 61 KGEISFSKTMAQIEAMLPPEMKTMAKEALTHCKDTQTSYKDPCDKAYFSAKCAADFTPDT 120 Query: 150 FMWP 153 FM+P Sbjct: 121 FMFP 124 >2CXI-A nrPDB Length = 348 Score = 29.3 bits (64), Expect = 0.67, Method: Composition-based stats. Identities = 13/32 (40%), Positives = 18/32 (56%) Query: 48 PKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKC 79 PKF + DL+RL F+ +DL+ Y KC Sbjct: 2 PKFDVSKSDLERLIGRSFSIEEWEDLVLYAKC 33 >4I9E-A nrPDB Length = 383 Score = 28.5 bits (62), Expect = 1.1, Method: Composition-based stats. Identities = 16/100 (16%), Positives = 38/100 (38%), Gaps = 12/100 (12%) Query: 32 MEQFLTSLDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNKKG 91 M+Q S+D R + + ++ L+ DL Y +S Sbjct: 156 MKQTYFSMDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAIS---------- 205 Query: 92 EFNAPKALAQLPHLVPPEMMEMSRKSVEACRDTHKQFKES 131 + KA + P++M + ++ C+++ Q++++ Sbjct: 206 --HFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYEDA 243 >5BJS-A nrPDB Length = 605 Score = 28.1 bits (61), Expect = 1.7, Method: Composition-based stats. Identities = 25/97 (25%), Positives = 32/97 (32%), Gaps = 9/97 (9%) Query: 39 LDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNKKGEFNAPKA 98 L+++ G P +L RLR F F + VS EF K Sbjct: 33 LEVLFQGPMPASDSAEWELPRLRTS-FIFQDDYKYLGDDGSVSQ------DLAEFFDVKF 85 Query: 99 LAQLPHLVPPEMMEMSRKSVEACRDTHKQFKE--SCE 133 P PP S+K CR T K+ CE Sbjct: 86 YPYSPPGAPPVFAATSKKHAVICRLTQTTDKDANPCE 122 >1C5G-A nrPDB Length = 402 Score = 27.7 bits (60), Expect = 1.9, Method: Composition-based stats. Identities = 18/81 (22%), Positives = 30/81 (37%), Gaps = 13/81 (16%) Query: 48 PKFKLKTE-------------DLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNKKGEFN 94 PKF L+TE D+ R DF Q+ + + + + VN+ G Sbjct: 299 PKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVA 358 Query: 95 APKALAQLPHLVPPEMMEMSR 115 + + + PE + M R Sbjct: 359 SSSTAVIVSARMAPEEIIMDR 379 >1INP-A nrPDB Length = 400 Score = 25.8 bits (55), Expect = 6.8, Method: Composition-based stats. Identities = 5/24 (20%), Positives = 11/24 (45%) Query: 130 ESCERVYQTAKCFSENADGQFMWP 153 + + YQ K ++ Q ++P Sbjct: 153 DPIDSTYQYIKGSADITPNQGIFP 176 >3LW2-A nrPDB Length = 379 Score = 25.4 bits (54), Expect = 9.6, Method: Composition-based stats. Identities = 17/93 (18%), Positives = 32/93 (34%), Gaps = 13/93 (13%) Query: 48 PKFKLKTE-------------DLDRLRVGDFNFPPSQDLMCYTKCVSLMAGTVNKKGEFN 94 PKF L+TE D+ + DF Q+ + + + + VN+ G Sbjct: 276 PKFSLETEVDLRGPLEKLGMPDMFSATLADFTSLSDQEQLSVAQALQKVRIEVNESGTVA 335 Query: 95 APKALAQLPHLVPPEMMEMSRKSVEACRDTHKQ 127 + + + P M + R + R + Sbjct: 336 SSSTAFVISARMAPTEMVIDRSFLFVVRHNPTE 368 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.132 0.380 Lambda K H 0.267 0.0401 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,464,325 Number of Sequences: 36641 Number of extensions: 183644 Number of successful extensions: 653 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 641 Number of HSP's gapped (non-prelim): 17 length of query: 153 length of database: 10,336,785 effective HSP length: 89 effective length of query: 64 effective length of database: 7,075,736 effective search space: 452847104 effective search space used: 452847104 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.0 bits) S2: 54 (25.4 bits)