BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= F4IEM5 (410 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3T33-A nrPDB 849 0.0 3E73-A nrPDB 240 1e-63 1CT9-A nrPDB 34 0.12 4AP9-A nrPDB 31 0.94 1VJT-A nrPDB 29 3.0 5HHZ-A nrPDB 29 3.8 3U95-A nrPDB 28 9.8 >3T33-A nrPDB Length = 415 Score = 849 bits (2193), Expect = 0.0, Method: Compositional matrix adjust. Identities = 410/410 (100%), Positives = 410/410 (100%) Query: 1 MGERFFRNEMPEFVPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVW 60 MGERFFRNEMPEFVPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVW Sbjct: 6 MGERFFRNEMPEFVPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVW 65 Query: 61 ETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVASRDSERVT 120 ETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVASRDSERVT Sbjct: 66 ETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVASRDSERVT 125 Query: 121 FICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWACLFLN 180 FICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWACLFLN Sbjct: 126 FICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWACLFLN 185 Query: 181 KHIGQESISSERMRSVVEEIFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMNVLMH 240 KHIGQESISSERMRSVVEEIFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMNVLMH Sbjct: 186 KHIGQESISSERMRSVVEEIFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMNVLMH 245 Query: 241 TELEPDEIKDVKGTLSYMIQNRFPSGNYLSSEGSKSDRLVHWCHGAPGVALTLVKAAQVY 300 TELEPDEIKDVKGTLSYMIQNRFPSGNYLSSEGSKSDRLVHWCHGAPGVALTLVKAAQVY Sbjct: 246 TELEPDEIKDVKGTLSYMIQNRFPSGNYLSSEGSKSDRLVHWCHGAPGVALTLVKAAQVY 305 Query: 301 NTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKAFASF 360 NTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKAFASF Sbjct: 306 NTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKAFASF 365 Query: 361 LLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL 410 LLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL Sbjct: 366 LLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL 415 >3E73-A nrPDB Length = 411 Score = 240 bits (612), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 146/373 (39%), Positives = 206/373 (55%), Gaps = 23/373 (6%) Query: 52 LSIKDKVVWETWERSGKRV--RDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEAC 109 L+ K + + + ER K RD YTG G A L Y V + L+L V+ Sbjct: 48 LTNKIRELLQQMERGLKSADPRDGTGYTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQ- 106 Query: 110 DVASRDSERVTFICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGI-RLPSDLPYELLYG 168 + +TF+CG AG A+ AV + +++ + + R + ++ P E+LYG Sbjct: 107 SLNCLTKRSITFLCGDAGPLAVAAVLYHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYG 166 Query: 169 RAGYLWACLFLNKHIGQESISSERMRSVVEEIFRAGRQLGNK----GTCPLMYEWHGKRY 224 R GY++A LF+NK+ G E I ++ + E I +G L K PLMYEW+ + Y Sbjct: 167 RIGYIYALLFVNKNFGVEKIPQSHIQQICETILTSGENLARKRNFTAKSPLMYEWYQEYY 226 Query: 225 WGAAHGLAGIMNVLMHTELEPDEIKD---VKGTLSYMIQNRFPSGNYLSSEGSKSDRLVH 281 GAAHGLAGI LM L+ + K VK ++ Y+ Q +FPSGNY G D LVH Sbjct: 227 VGAAHGLAGIYYYLMQPSLQVSQGKLHSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVH 286 Query: 282 WCHGAPGVALTLVKAAQVYNTKEFVEAAMEAGEVVWSRGLLKR-VGICHGISGNTYVFLS 340 WCHGAPGV L++A +V+ ++++ A + +V+W GLLK+ G+CHG +GN Y FL+ Sbjct: 287 WCHGAPGVIYMLIQAYKVFREEKYLCDAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLT 346 Query: 341 LYRLTRNPKYLYRAKAFASFLLDKSEKLISEGQMHG---GDRPFSLFEGIGGMAYMLLDM 397 LY LT++ KYLYRA FA + L+ E HG D PFSLFEG+ G Y L D+ Sbjct: 347 LYNLTQDMKYLYRACKFAEWCLEYGE--------HGCRTPDTPFSLFEGMAGTIYFLADL 398 Query: 398 NDPTQALFPGYEL 410 PT+A FP +EL Sbjct: 399 LVPTKARFPAFEL 411 >1CT9-A nrPDB Length = 553 Score = 33.9 bits (76), Expect = 0.12, Method: Compositional matrix adjust. Identities = 25/80 (31%), Positives = 35/80 (43%), Gaps = 10/80 (12%) Query: 99 LKLCLENVEACDVASRDSERVTFICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLP 158 L+ C E VA R E+ + GY+ + L VAA+ + D QL ARFR Sbjct: 432 LRECFEAYLPASVAWRQKEQFSDGVGYSWIDTLKEVAAQQVSDQQL---ETARFR----- 483 Query: 159 SDLPYELLYGRAGYLWACLF 178 PY + YL+ +F Sbjct: 484 --FPYNTPTSKEAYLYREIF 501 >4AP9-A nrPDB Length = 201 Score = 31.2 bits (69), Expect = 0.94, Method: Compositional matrix adjust. Identities = 15/37 (40%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Query: 301 NTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYV 337 +E +E + +GEV W LLKRVG+ GI T++ Sbjct: 37 EIEELLEKGL-SGEVEWLDSLLKRVGLIRGIDEGTFL 72 >1VJT-A nrPDB Length = 483 Score = 29.3 bits (64), Expect = 3.0, Method: Compositional matrix adjust. Identities = 16/46 (34%), Positives = 23/46 (50%), Gaps = 1/46 (2%) Query: 219 WHGKRYWGAAHGLAGIMNVLMHTELEPDEIK-DVKGTLSYMIQNRF 263 W G G HG+AG+ V +L+P+E+ V G + NRF Sbjct: 184 WTGANIVGFCHGVAGVYEVFEKLDLDPEEVDWQVAGVNHGIWLNRF 229 >5HHZ-A nrPDB Length = 524 Score = 28.9 bits (63), Expect = 3.8, Method: Compositional matrix adjust. Identities = 29/124 (23%), Positives = 54/124 (43%), Gaps = 31/124 (25%) Query: 38 LPYKSFSEKLHRYALSIKDKVVWET---WER---SGKRVRDYN--LYTGVL-------GT 82 LPY F +++H+ L ++ K +WE W R+ D++ ++ GVL G Sbjct: 344 LPYVDFLDRVHKAELKLRAKGMWEVPHPWLNLFVPASRIADFDRGVFRGVLGGRTAGAGG 403 Query: 83 AYLLF---------KSYQVTRNEDD------LKLCLENV-EACDVASRDSERVTFICGYA 126 L++ +S VT +E+ L+ L E+ + +R ++R+ C Sbjct: 404 PVLIYPMNKHKWDPRSSAVTPDEEVFYLVAFLRSALPGAPESLEALARQNQRILDFCAGT 463 Query: 127 GVCA 130 G+ A Sbjct: 464 GIGA 467 >3U95-A nrPDB Length = 477 Score = 27.7 bits (60), Expect = 9.8, Method: Compositional matrix adjust. Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%) Query: 219 WHGKRYWGAAHGLAGIMNVLMHTELEPDEIK-DVKGTLSYMIQNRF 263 W G G HG+AG+ V L+P+E+ V G + NRF Sbjct: 172 WTGANIIGFCHGVAGVYEVFERLGLDPEEVDWQVAGVNHGIWLNRF 217 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3T33-A nrPDB 721 0.0 3E73-A nrPDB 568 e-162 Sequences not found previously or not previously below threshold: 6FWS-A nrPDB 30 2.0 1VJT-A nrPDB 29 4.3 5GK9-A nrPDB 29 4.9 4DA1-A nrPDB 28 7.0 2IQ1-A nrPDB 28 7.0 3U95-A nrPDB 28 9.6 CONVERGED! >3T33-A nrPDB Length = 415 Score = 721 bits (1861), Expect = 0.0, Method: Composition-based stats. Identities = 410/410 (100%), Positives = 410/410 (100%) Query: 1 MGERFFRNEMPEFVPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVW 60 MGERFFRNEMPEFVPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVW Sbjct: 6 MGERFFRNEMPEFVPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVW 65 Query: 61 ETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVASRDSERVT 120 ETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVASRDSERVT Sbjct: 66 ETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVASRDSERVT 125 Query: 121 FICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWACLFLN 180 FICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWACLFLN Sbjct: 126 FICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWACLFLN 185 Query: 181 KHIGQESISSERMRSVVEEIFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMNVLMH 240 KHIGQESISSERMRSVVEEIFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMNVLMH Sbjct: 186 KHIGQESISSERMRSVVEEIFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMNVLMH 245 Query: 241 TELEPDEIKDVKGTLSYMIQNRFPSGNYLSSEGSKSDRLVHWCHGAPGVALTLVKAAQVY 300 TELEPDEIKDVKGTLSYMIQNRFPSGNYLSSEGSKSDRLVHWCHGAPGVALTLVKAAQVY Sbjct: 246 TELEPDEIKDVKGTLSYMIQNRFPSGNYLSSEGSKSDRLVHWCHGAPGVALTLVKAAQVY 305 Query: 301 NTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKAFASF 360 NTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKAFASF Sbjct: 306 NTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKAFASF 365 Query: 361 LLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL 410 LLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL Sbjct: 366 LLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL 415 >3E73-A nrPDB Length = 411 Score = 568 bits (1463), Expect = e-162, Method: Composition-based stats. Identities = 144/370 (38%), Positives = 204/370 (55%), Gaps = 17/370 (4%) Query: 52 LSIKDKVVWETWERSGKRV--RDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEAC 109 L+ K + + + ER K RD YTG G A L Y V + L+L V+ Sbjct: 48 LTNKIRELLQQMERGLKSADPRDGTGYTGWAGIAVLYLHLYDVFGDPAYLQLAHGYVKQ- 106 Query: 110 DVASRDSERVTFICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGI-RLPSDLPYELLYG 168 + +TF+CG AG A+ AV + +++ + + R + ++ P E+LYG Sbjct: 107 SLNCLTKRSITFLCGDAGPLAVAAVLYHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYG 166 Query: 169 RAGYLWACLFLNKHIGQESISSERMRSVVEEIFRAGRQLGNK----GTCPLMYEWHGKRY 224 R GY++A LF+NK+ G E I ++ + E I +G L K PLMYEW+ + Y Sbjct: 167 RIGYIYALLFVNKNFGVEKIPQSHIQQICETILTSGENLARKRNFTAKSPLMYEWYQEYY 226 Query: 225 WGAAHGLAGIMNVLMHTELEPDEIKD---VKGTLSYMIQNRFPSGNYLSSEGSKSDRLVH 281 GAAHGLAGI LM L+ + K VK ++ Y+ Q +FPSGNY G D LVH Sbjct: 227 VGAAHGLAGIYYYLMQPSLQVSQGKLHSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVH 286 Query: 282 WCHGAPGVALTLVKAAQVYNTKEFVEAAMEAGEVVWSRGLLKR-VGICHGISGNTYVFLS 340 WCHGAPGV L++A +V+ ++++ A + +V+W GLLK+ G+CHG +GN Y FL+ Sbjct: 287 WCHGAPGVIYMLIQAYKVFREEKYLCDAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLT 346 Query: 341 LYRLTRNPKYLYRAKAFASFLLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDP 400 LY LT++ KYLYRA FA + L+ E D PFSLFEG+ G Y L D+ P Sbjct: 347 LYNLTQDMKYLYRACKFAEWCLEYGEHGCRT-----PDTPFSLFEGMAGTIYFLADLLVP 401 Query: 401 TQALFPGYEL 410 T+A FP +EL Sbjct: 402 TKARFPAFEL 411 >6FWS-A nrPDB Length = 716 Score = 30.0 bits (66), Expect = 2.0, Method: Composition-based stats. Identities = 20/67 (29%), Positives = 31/67 (46%), Gaps = 1/67 (1%) Query: 3 ERFFRNEMPEF-VPEDLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKDKVVWE 61 E+F +P +PE L+ E + E SL +L+L + E HR+A+ V E Sbjct: 288 EQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEHRFAMGELPDEVLE 347 Query: 62 TWERSGK 68 +R K Sbjct: 348 ICQRLAK 354 >1VJT-A nrPDB Length = 483 Score = 28.8 bits (63), Expect = 4.3, Method: Composition-based stats. Identities = 11/30 (36%), Positives = 17/30 (56%) Query: 219 WHGKRYWGAAHGLAGIMNVLMHTELEPDEI 248 W G G HG+AG+ V +L+P+E+ Sbjct: 184 WTGANIVGFCHGVAGVYEVFEKLDLDPEEV 213 >5GK9-A nrPDB Length = 276 Score = 28.8 bits (63), Expect = 4.9, Method: Composition-based stats. Identities = 16/51 (31%), Positives = 23/51 (45%), Gaps = 6/51 (11%) Query: 160 DLPYELLYGRAGYLWACLFLNKHIGQESISSERMRSVV------EEIFRAG 204 PY Y R G L+ C F K++ ++I M V +EI+R G Sbjct: 17 HSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVWKHPPGDEIYRKG 67 >4DA1-A nrPDB Length = 389 Score = 28.0 bits (61), Expect = 7.0, Method: Composition-based stats. Identities = 7/35 (20%), Positives = 11/35 (31%) Query: 84 YLLFKSYQVTRNEDDLKLCLENVEACDVASRDSER 118 L F Y C ++E C + E+ Sbjct: 149 VLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEK 183 >2IQ1-A nrPDB Length = 274 Score = 28.0 bits (61), Expect = 7.0, Method: Composition-based stats. Identities = 7/35 (20%), Positives = 11/35 (31%) Query: 84 YLLFKSYQVTRNEDDLKLCLENVEACDVASRDSER 118 L F Y C ++E C + E+ Sbjct: 35 VLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEK 69 >3U95-A nrPDB Length = 477 Score = 27.7 bits (60), Expect = 9.6, Method: Composition-based stats. Identities = 11/30 (36%), Positives = 16/30 (53%) Query: 219 WHGKRYWGAAHGLAGIMNVLMHTELEPDEI 248 W G G HG+AG+ V L+P+E+ Sbjct: 172 WTGANIIGFCHGVAGVYEVFERLGLDPEEV 201 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.140 0.413 Lambda K H 0.267 0.0430 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 17,327,223 Number of Sequences: 36641 Number of extensions: 735444 Number of successful extensions: 1889 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 18 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 1845 Number of HSP's gapped (non-prelim): 27 length of query: 410 length of database: 10,336,785 effective HSP length: 99 effective length of query: 311 effective length of database: 6,709,326 effective search space: 2086600386 effective search space used: 2086600386 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 60 (27.7 bits)