BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= F1RAX4 (670 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5SY1-A nrPDB 1372 0.0 4BG9-A nrPDB 33 0.36 6BOA-A nrPDB 30 2.7 >5SY1-A nrPDB Length = 670 Score = 1372 bits (3551), Expect = 0.0, Method: Compositional matrix adjust. Identities = 667/670 (99%), Positives = 668/670 (99%) Query: 1 MSAETVNNYDYSDWYENAAPTKAPVEVIPPCDPTADEGLFHICIAAISLVVMLVLAILAR 60 MSAETVNNYDYSDWYENAAPTKAPVEVIPPCDPTADEGLFHICIAAISLVVMLVLAILAR Sbjct: 1 MSAETVNNYDYSDWYENAAPTKAPVEVIPPCDPTADEGLFHICIAAISLVVMLVLAILAR 60 Query: 61 RQKLSDNQRGLTGLLSPVNFLDHTQHKGLAVAVYGVLFCKLVGMVLSHHPLPFTKEVANK 120 RQKLSDNQRGLTGLLSPVNFLDHTQHKGLAVAVYGVLFCKLVGMVLSHHPLPFTKEVANK Sbjct: 61 RQKLSDNQRGLTGLLSPVNFLDHTQHKGLAVAVYGVLFCKLVGMVLSHHPLPFTKEVANK 120 Query: 121 EFWMILALLYYPALYYPLLACGTLHNKVGYVLGSLLSWTHFGILVWQKVDCPKTPQIYKY 180 EFWMILALLYYP LYYPLLACGTLHNKVGYVLGSLLSWTHFGILVWQKVDCPKTPQIYKY Sbjct: 121 EFWMILALLYYPTLYYPLLACGTLHNKVGYVLGSLLSWTHFGILVWQKVDCPKTPQIYKY 180 Query: 181 YALFGSLPQIACLAFLSFQYPLLLFKGLQNTETANASEDLSSSYYRDYVKKILKKKKPTK 240 YALFGSLPQIACLAFLSFQYPLLLFKGLQNTETANASEDLSSSYYRDYVKKILKKKKPTK Sbjct: 181 YALFGSLPQIACLAFLSFQYPLLLFKGLQNTETANASEDLSSSYYRDYVKKILKKKKPTK 240 Query: 241 ISSSTSKPKLFDRLRDAVKSYIYTPEDVFRFPLKLAISVVVAFIALYQMALLLISGVLPT 300 ISSSTSKPKLFDRLRDAVKSYIYTPEDVFRFPLKLAISVVVAFIALYQMALLLISGVLPT Sbjct: 241 ISSSTSKPKLFDRLRDAVKSYIYTPEDVFRFPLKLAISVVVAFIALYQMALLLISGVLPT 300 Query: 301 LHIVRRGVDENIAFLLAGFNIILSNDRQEVVRIVVYYLWCVEICYVSAVTLSCLVNLLML 360 LHIVRRGVDENIAFLLAGFNIILSNDRQEVVRIVVYYLWCVEICYVSAVTLSCLVNLLML Sbjct: 301 LHIVRRGVDENIAFLLAGFNIILSNDRQEVVRIVVYYLWCVEICYVSAVTLSCLVNLLML 360 Query: 361 MRSMVLHRSNLKGLYRGDSLNVFNCHRSIRPSRPALVCWMGFTSYQAAFLCLGMAIQTLV 420 MRSMVLHRSNLKGLYRGDSLNVFNCHRSIRPSRPALVCWMGFTSYQAAFLCLGMAIQTLV Sbjct: 361 MRSMVLHRSNLKGLYRGDSLNVFNCHRSIRPSRPALVCWMGFTSYQAAFLCLGMAIQTLV 420 Query: 421 FFICILFAVFLIIIPILWGTNLMLFHIIGNLWPFWLTLVLAALIQHVASRFLFIRKDGGT 480 FFICILFAVFLIIIPILWGTNLMLFHIIGNLWPFWLTLVLAALIQHVASRFLFIRKDGGT Sbjct: 421 FFICILFAVFLIIIPILWGTNLMLFHIIGNLWPFWLTLVLAALIQHVASRFLFIRKDGGT 480 Query: 481 RDLNNRGSLFLLSYILFLVNVMIGVVLGIWRVVITALFNIVHLGRLDISLLNRNVEAFDP 540 RDLNNRGSLFLLSYILFLVNVMIGVVLGIWRVVITALFNIVHLGRLDISLLNRNVEAFDP Sbjct: 481 RDLNNRGSLFLLSYILFLVNVMIGVVLGIWRVVITALFNIVHLGRLDISLLNRNVEAFDP 540 Query: 541 GYRCYSHYLKIEVSQSHPVMKAFCGLLLQSSGQDGLSAQRIRDAEEGIQLVQQEKKQNKV 600 GYRCYSHYLKIEVSQSHPVMKAFCGLLLQSSGQDGLSAQRIRDAEEGIQLVQQEKKQNKV Sbjct: 541 GYRCYSHYLKIEVSQSHPVMKAFCGLLLQSSGQDGLSAQRIRDAEEGIQLVQQEKKQNKV 600 Query: 601 SNAKRARAHWQLLYTLVNNPSLVGSRKHFQCQSSESFINGALSRTSKEGSKKDGSVNEPS 660 SNAKRARAHWQLLYTLVNNPSLVGSRKHFQCQSSESFINGALSRTSKEGSKKDGSV EP+ Sbjct: 601 SNAKRARAHWQLLYTLVNNPSLVGSRKHFQCQSSESFINGALSRTSKEGSKKDGSVKEPN 660 Query: 661 KEAESAAASN 670 KEAESAAASN Sbjct: 661 KEAESAAASN 670 >4BG9-A nrPDB Length = 361 Score = 33.1 bits (74), Expect = 0.36, Method: Compositional matrix adjust. Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 4/100 (4%) Query: 215 NASEDLSSSYYRDYVKKILKKKKPT----KISSSTSKPKLFDRLRDAVKSYIYTPEDVFR 270 A+E +R ++ + KP + S + P+ LRD ++ EDV R Sbjct: 215 KAAEGEVDENFRRELRSVDVDGKPVFGRVRGSLAPVPPEQERVLRDHIRDAGAPAEDVLR 274 Query: 271 FPLKLAISVVVAFIALYQMALLLISGVLPTLHIVRRGVDE 310 ++L +V A Y M LL++SG +++R V E Sbjct: 275 TLVELVAETIVINAAQYDMDLLVLSGGGVKNELLKRRVSE 314 >6BOA-A nrPDB Length = 742 Score = 30.4 bits (67), Expect = 2.7, Method: Compositional matrix adjust. Identities = 21/64 (32%), Positives = 29/64 (45%) Query: 23 APVEVIPPCDPTADEGLFHICIAAISLVVMLVLAILARRQKLSDNQRGLTGLLSPVNFLD 82 AP V P EG + IA ++ + LV A+LARR +S G SP N + Sbjct: 102 APELVFEPMTSELYEGQTALHIAVVNQNMNLVRALLARRASVSARATGTAFRRSPCNLIY 161 Query: 83 HTQH 86 +H Sbjct: 162 FGEH 165 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5SY1-A nrPDB 1314 0.0 Sequences not found previously or not previously below threshold: 4BG9-A nrPDB 30 3.1 6BOA-A nrPDB 29 5.7 CONVERGED! >5SY1-A nrPDB Length = 670 Score = 1314 bits (3402), Expect = 0.0, Method: Composition-based stats. Identities = 667/670 (99%), Positives = 668/670 (99%) Query: 1 MSAETVNNYDYSDWYENAAPTKAPVEVIPPCDPTADEGLFHICIAAISLVVMLVLAILAR 60 MSAETVNNYDYSDWYENAAPTKAPVEVIPPCDPTADEGLFHICIAAISLVVMLVLAILAR Sbjct: 1 MSAETVNNYDYSDWYENAAPTKAPVEVIPPCDPTADEGLFHICIAAISLVVMLVLAILAR 60 Query: 61 RQKLSDNQRGLTGLLSPVNFLDHTQHKGLAVAVYGVLFCKLVGMVLSHHPLPFTKEVANK 120 RQKLSDNQRGLTGLLSPVNFLDHTQHKGLAVAVYGVLFCKLVGMVLSHHPLPFTKEVANK Sbjct: 61 RQKLSDNQRGLTGLLSPVNFLDHTQHKGLAVAVYGVLFCKLVGMVLSHHPLPFTKEVANK 120 Query: 121 EFWMILALLYYPALYYPLLACGTLHNKVGYVLGSLLSWTHFGILVWQKVDCPKTPQIYKY 180 EFWMILALLYYP LYYPLLACGTLHNKVGYVLGSLLSWTHFGILVWQKVDCPKTPQIYKY Sbjct: 121 EFWMILALLYYPTLYYPLLACGTLHNKVGYVLGSLLSWTHFGILVWQKVDCPKTPQIYKY 180 Query: 181 YALFGSLPQIACLAFLSFQYPLLLFKGLQNTETANASEDLSSSYYRDYVKKILKKKKPTK 240 YALFGSLPQIACLAFLSFQYPLLLFKGLQNTETANASEDLSSSYYRDYVKKILKKKKPTK Sbjct: 181 YALFGSLPQIACLAFLSFQYPLLLFKGLQNTETANASEDLSSSYYRDYVKKILKKKKPTK 240 Query: 241 ISSSTSKPKLFDRLRDAVKSYIYTPEDVFRFPLKLAISVVVAFIALYQMALLLISGVLPT 300 ISSSTSKPKLFDRLRDAVKSYIYTPEDVFRFPLKLAISVVVAFIALYQMALLLISGVLPT Sbjct: 241 ISSSTSKPKLFDRLRDAVKSYIYTPEDVFRFPLKLAISVVVAFIALYQMALLLISGVLPT 300 Query: 301 LHIVRRGVDENIAFLLAGFNIILSNDRQEVVRIVVYYLWCVEICYVSAVTLSCLVNLLML 360 LHIVRRGVDENIAFLLAGFNIILSNDRQEVVRIVVYYLWCVEICYVSAVTLSCLVNLLML Sbjct: 301 LHIVRRGVDENIAFLLAGFNIILSNDRQEVVRIVVYYLWCVEICYVSAVTLSCLVNLLML 360 Query: 361 MRSMVLHRSNLKGLYRGDSLNVFNCHRSIRPSRPALVCWMGFTSYQAAFLCLGMAIQTLV 420 MRSMVLHRSNLKGLYRGDSLNVFNCHRSIRPSRPALVCWMGFTSYQAAFLCLGMAIQTLV Sbjct: 361 MRSMVLHRSNLKGLYRGDSLNVFNCHRSIRPSRPALVCWMGFTSYQAAFLCLGMAIQTLV 420 Query: 421 FFICILFAVFLIIIPILWGTNLMLFHIIGNLWPFWLTLVLAALIQHVASRFLFIRKDGGT 480 FFICILFAVFLIIIPILWGTNLMLFHIIGNLWPFWLTLVLAALIQHVASRFLFIRKDGGT Sbjct: 421 FFICILFAVFLIIIPILWGTNLMLFHIIGNLWPFWLTLVLAALIQHVASRFLFIRKDGGT 480 Query: 481 RDLNNRGSLFLLSYILFLVNVMIGVVLGIWRVVITALFNIVHLGRLDISLLNRNVEAFDP 540 RDLNNRGSLFLLSYILFLVNVMIGVVLGIWRVVITALFNIVHLGRLDISLLNRNVEAFDP Sbjct: 481 RDLNNRGSLFLLSYILFLVNVMIGVVLGIWRVVITALFNIVHLGRLDISLLNRNVEAFDP 540 Query: 541 GYRCYSHYLKIEVSQSHPVMKAFCGLLLQSSGQDGLSAQRIRDAEEGIQLVQQEKKQNKV 600 GYRCYSHYLKIEVSQSHPVMKAFCGLLLQSSGQDGLSAQRIRDAEEGIQLVQQEKKQNKV Sbjct: 541 GYRCYSHYLKIEVSQSHPVMKAFCGLLLQSSGQDGLSAQRIRDAEEGIQLVQQEKKQNKV 600 Query: 601 SNAKRARAHWQLLYTLVNNPSLVGSRKHFQCQSSESFINGALSRTSKEGSKKDGSVNEPS 660 SNAKRARAHWQLLYTLVNNPSLVGSRKHFQCQSSESFINGALSRTSKEGSKKDGSV EP+ Sbjct: 601 SNAKRARAHWQLLYTLVNNPSLVGSRKHFQCQSSESFINGALSRTSKEGSKKDGSVKEPN 660 Query: 661 KEAESAAASN 670 KEAESAAASN Sbjct: 661 KEAESAAASN 670 >4BG9-A nrPDB Length = 361 Score = 30.3 bits (67), Expect = 3.1, Method: Composition-based stats. Identities = 19/57 (33%), Positives = 29/57 (50%) Query: 254 LRDAVKSYIYTPEDVFRFPLKLAISVVVAFIALYQMALLLISGVLPTLHIVRRGVDE 310 LRD ++ EDV R ++L +V A Y M LL++SG +++R V E Sbjct: 258 LRDHIRDAGAPAEDVLRTLVELVAETIVINAAQYDMDLLVLSGGGVKNELLKRRVSE 314 >6BOA-A nrPDB Length = 742 Score = 29.1 bits (64), Expect = 5.7, Method: Composition-based stats. Identities = 21/64 (32%), Positives = 29/64 (45%) Query: 23 APVEVIPPCDPTADEGLFHICIAAISLVVMLVLAILARRQKLSDNQRGLTGLLSPVNFLD 82 AP V P EG + IA ++ + LV A+LARR +S G SP N + Sbjct: 102 APELVFEPMTSELYEGQTALHIAVVNQNMNLVRALLARRASVSARATGTAFRRSPCNLIY 161 Query: 83 HTQH 86 +H Sbjct: 162 FGEH 165 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.323 0.148 0.478 Lambda K H 0.267 0.0449 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 31,580,680 Number of Sequences: 36641 Number of extensions: 1506231 Number of successful extensions: 4127 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 4120 Number of HSP's gapped (non-prelim): 22 length of query: 670 length of database: 10,336,785 effective HSP length: 104 effective length of query: 566 effective length of database: 6,526,121 effective search space: 3693784486 effective search space used: 3693784486 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.4 bits) S2: 63 (28.7 bits)