BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= F1QXD3 (332 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6BMS-A nrPDB 688 0.0 4QBG-B nrPDB 30 1.6 3NVN-B nrPDB 30 2.0 5AN8-A nrPDB 28 5.7 >6BMS-A nrPDB Length = 341 Score = 688 bits (1776), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/332 (99%), Positives = 331/332 (99%) Query: 1 MALSRALRCCQRIFSWIPVIIISSVVLWSYYAYVFELCFVTLSNNLERVTYLLIFHVCFI 60 MALSRALRCCQRIFSWIPVIIISSVVLWSYYAYVFELCFVTLSNNLERVTYLLIFHVCFI Sbjct: 7 MALSRALRCCQRIFSWIPVIIISSVVLWSYYAYVFELCFVTLSNNLERVTYLLIFHVCFI 66 Query: 61 MFCWTYWKAIFTPPSTPTKKFHLSYTDKERYEMEERPEVQKQILVDIAKKLPIFTRAQSG 120 MFCWTYWKAIFTPPSTPTKKFHLSYTDKERYEMEERPEVQKQILVDIAKKLPIFTRAQSG Sbjct: 67 MFCWTYWKAIFTPPSTPTKKFHLSYTDKERYEMEERPEVQKQILVDIAKKLPIFTRAQSG 126 Query: 121 AIRFCDRCQVIKPDRCHHCSVCETCVLKMDHHCPWVNNCVGFSNYKFFLLFLSYSMIYCV 180 AIRFCDRCQVIKPDRCHHCSVCETCVLKMDHH PWVNNCVGFSNYKFFLLFLSYSMIYCV Sbjct: 127 AIRFCDRCQVIKPDRCHHCSVCETCVLKMDHHSPWVNNCVGFSNYKFFLLFLSYSMIYCV 186 Query: 181 FIASTVFQYFLKFWVGDLPNGPAKFHVLFLLFVALMFFVSLMFLFGYHCWLVAKNRSTLE 240 FIASTVFQYFLKFWVGDLPNGPAKFHVLFLLFVALMFFVSLMFLFGYHCWLVAKNRSTLE Sbjct: 187 FIASTVFQYFLKFWVGDLPNGPAKFHVLFLLFVALMFFVSLMFLFGYHCWLVAKNRSTLE 246 Query: 241 AFSPPVFQNGPDRNGFNVGLSKNLRQVFGEHKKLWFIPVFTSQGDGHYFPLRTLRESENP 300 AFSPPVFQNGPDRNGFNVGLSKNLRQVFGEHKKLWFIPVFTSQGDGHYFPLRTLRESENP Sbjct: 247 AFSPPVFQNGPDRNGFNVGLSKNLRQVFGEHKKLWFIPVFTSQGDGHYFPLRTLRESENP 306 Query: 301 LLANEEKWVEDGGSDEESADENGSSLLIRTES 332 LLANEEKWVEDGGSDEESADENGSSLLIRTES Sbjct: 307 LLANEEKWVEDGGSDEESADENGSSLLIRTES 338 >4QBG-B nrPDB Length = 217 Score = 30.0 bits (66), Expect = 1.6, Method: Compositional matrix adjust. Identities = 22/87 (25%), Positives = 41/87 (47%), Gaps = 14/87 (16%) Query: 46 LERVTYLLIFHVCFIMFCWTYWKAIFTPPSTPTKKFHLSYTDKERYEMEERPEVQKQILV 105 +ER+T I VC T + +F PP TP DK+ E+ +R + ++ Sbjct: 121 MERLTGRRICSVC-----GTTYHLVFNPPKTP------GICDKDGGELYQRADDNEET-- 167 Query: 106 DIAKKLPIFTRAQSGAIRFCDRCQVIK 132 +AK+L + + + + F D +V++ Sbjct: 168 -VAKRLEVNMKQMAPLLAFYDSKEVLR 193 >3NVN-B nrPDB Length = 476 Score = 29.6 bits (65), Expect = 2.0, Method: Compositional matrix adjust. Identities = 26/114 (22%), Positives = 42/114 (36%), Gaps = 25/114 (21%) Query: 91 YEMEERPEVQKQILVDIAKKLPIFTRAQSGAIRFCDRCQVIKPDRCHHCSVCETCVLKMD 150 YE++E V +++ D K + I+ A R I+ C+ C C+ D Sbjct: 383 YEIKEETPVFYKLVPDPVKNIYIYLTAGKEVRR-------IRVANCNKHKSCSECLTATD 435 Query: 151 HHCPWVNNCVGFSNYKFFLLFLSYSMIYCVFIASTVFQYFLKFWVGDLPNGPAK 204 HC W +S+ C F V L+ W+ D+ +G K Sbjct: 436 PHCGWC-----------------HSLQRCTFQGDCVHSENLENWL-DISSGAKK 471 >5AN8-A nrPDB Length = 613 Score = 28.1 bits (61), Expect = 5.7, Method: Compositional matrix adjust. Identities = 20/85 (23%), Positives = 33/85 (38%), Gaps = 23/85 (27%) Query: 161 GFSNYKFFLLFLSYSMIYCVFIASTVFQYFLKFWVGDLPNGPAKFHVLFLLFVALMFFVS 220 G+ N ++ ++ IY V I + + L+F V Sbjct: 431 GWLNLLYYTRGFQHTGIYSVMIQKVILRDLLRF-----------------------LLVY 467 Query: 221 LMFLFGYHCWLVAKNRSTLEAFSPP 245 L+FLFG+ LV+ +R E +PP Sbjct: 468 LVFLFGFAVALVSLSREAQEDEAPP 492 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6BMS-A nrPDB 666 0.0 Sequences not found previously or not previously below threshold: 5LNK-M nrPDB 30 2.2 4QBG-B nrPDB 29 3.1 3NVN-B nrPDB 28 4.5 CONVERGED! >6BMS-A nrPDB Length = 341 Score = 666 bits (1718), Expect = 0.0, Method: Composition-based stats. Identities = 331/332 (99%), Positives = 331/332 (99%) Query: 1 MALSRALRCCQRIFSWIPVIIISSVVLWSYYAYVFELCFVTLSNNLERVTYLLIFHVCFI 60 MALSRALRCCQRIFSWIPVIIISSVVLWSYYAYVFELCFVTLSNNLERVTYLLIFHVCFI Sbjct: 7 MALSRALRCCQRIFSWIPVIIISSVVLWSYYAYVFELCFVTLSNNLERVTYLLIFHVCFI 66 Query: 61 MFCWTYWKAIFTPPSTPTKKFHLSYTDKERYEMEERPEVQKQILVDIAKKLPIFTRAQSG 120 MFCWTYWKAIFTPPSTPTKKFHLSYTDKERYEMEERPEVQKQILVDIAKKLPIFTRAQSG Sbjct: 67 MFCWTYWKAIFTPPSTPTKKFHLSYTDKERYEMEERPEVQKQILVDIAKKLPIFTRAQSG 126 Query: 121 AIRFCDRCQVIKPDRCHHCSVCETCVLKMDHHCPWVNNCVGFSNYKFFLLFLSYSMIYCV 180 AIRFCDRCQVIKPDRCHHCSVCETCVLKMDHH PWVNNCVGFSNYKFFLLFLSYSMIYCV Sbjct: 127 AIRFCDRCQVIKPDRCHHCSVCETCVLKMDHHSPWVNNCVGFSNYKFFLLFLSYSMIYCV 186 Query: 181 FIASTVFQYFLKFWVGDLPNGPAKFHVLFLLFVALMFFVSLMFLFGYHCWLVAKNRSTLE 240 FIASTVFQYFLKFWVGDLPNGPAKFHVLFLLFVALMFFVSLMFLFGYHCWLVAKNRSTLE Sbjct: 187 FIASTVFQYFLKFWVGDLPNGPAKFHVLFLLFVALMFFVSLMFLFGYHCWLVAKNRSTLE 246 Query: 241 AFSPPVFQNGPDRNGFNVGLSKNLRQVFGEHKKLWFIPVFTSQGDGHYFPLRTLRESENP 300 AFSPPVFQNGPDRNGFNVGLSKNLRQVFGEHKKLWFIPVFTSQGDGHYFPLRTLRESENP Sbjct: 247 AFSPPVFQNGPDRNGFNVGLSKNLRQVFGEHKKLWFIPVFTSQGDGHYFPLRTLRESENP 306 Query: 301 LLANEEKWVEDGGSDEESADENGSSLLIRTES 332 LLANEEKWVEDGGSDEESADENGSSLLIRTES Sbjct: 307 LLANEEKWVEDGGSDEESADENGSSLLIRTES 338 >5LNK-M nrPDB Length = 459 Score = 29.5 bits (65), Expect = 2.2, Method: Composition-based stats. Identities = 18/62 (29%), Positives = 30/62 (48%), Gaps = 3/62 (4%) Query: 170 LFLSYSMIYCVFIASTVFQYFLKFWVGDLPNGPAKFHVLFLLFVALMFFVSLMFLFGYHC 229 L L ++IY ++ L++WV +PN + F+ +M F+ M L+G H Sbjct: 158 LPLLVALIYIQNTMGSLNFLILQYWVQPMPNSWSN---TFMWLACMMAFMVKMPLYGLHL 214 Query: 230 WL 231 WL Sbjct: 215 WL 216 >4QBG-B nrPDB Length = 217 Score = 29.1 bits (64), Expect = 3.1, Method: Composition-based stats. Identities = 22/87 (25%), Positives = 41/87 (47%), Gaps = 14/87 (16%) Query: 46 LERVTYLLIFHVCFIMFCWTYWKAIFTPPSTPTKKFHLSYTDKERYEMEERPEVQKQILV 105 +ER+T I VC T + +F PP TP DK+ E+ +R + ++ Sbjct: 121 MERLTGRRICSVC-----GTTYHLVFNPPKTP------GICDKDGGELYQRADDNEET-- 167 Query: 106 DIAKKLPIFTRAQSGAIRFCDRCQVIK 132 +AK+L + + + + F D +V++ Sbjct: 168 -VAKRLEVNMKQMAPLLAFYDSKEVLR 193 >3NVN-B nrPDB Length = 476 Score = 28.3 bits (62), Expect = 4.5, Method: Composition-based stats. Identities = 26/114 (22%), Positives = 42/114 (36%), Gaps = 25/114 (21%) Query: 91 YEMEERPEVQKQILVDIAKKLPIFTRAQSGAIRFCDRCQVIKPDRCHHCSVCETCVLKMD 150 YE++E V +++ D K + I+ A R I+ C+ C C+ D Sbjct: 383 YEIKEETPVFYKLVPDPVKNIYIYLTAGKEVRR-------IRVANCNKHKSCSECLTATD 435 Query: 151 HHCPWVNNCVGFSNYKFFLLFLSYSMIYCVFIASTVFQYFLKFWVGDLPNGPAK 204 HC W +S+ C F V L+ W+ D+ +G K Sbjct: 436 PHCGWC-----------------HSLQRCTFQGDCVHSENLENWL-DISSGAKK 471 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.322 0.148 0.515 Lambda K H 0.267 0.0447 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 16,858,255 Number of Sequences: 36641 Number of extensions: 808083 Number of successful extensions: 2393 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 28 Number of HSP's that attempted gapping in prelim test: 2386 Number of HSP's gapped (non-prelim): 37 length of query: 332 length of database: 10,336,785 effective HSP length: 97 effective length of query: 235 effective length of database: 6,782,608 effective search space: 1593912880 effective search space used: 1593912880 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.3 bits) S2: 60 (27.6 bits)