BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= D2ZZC1
         (263 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

4QTN-A nrPDB                                                          470   e-133

>4QTN-A nrPDB
          Length = 244

 Score =  470 bits (1209), Expect = e-133,   Method: Compositional matrix adjust.
 Identities = 236/236 (100%), Positives = 236/236 (100%)

Query: 28  SLAWWKRELFGGWTHFEAVWLLMFLGIQAVVFVFNPDSWLASVAAVTGILCVVFVGKGKI 87
           SLAWWKRELFGGWTHFEAVWLLMFLGIQAVVFVFNPDSWLASVAAVTGILCVVFVGKGKI
Sbjct: 3   SLAWWKRELFGGWTHFEAVWLLMFLGIQAVVFVFNPDSWLASVAAVTGILCVVFVGKGKI 62

Query: 88  SNYLFGLISVSLYAYVSYTFKLYGEMMLNLLVYVPVQFVGFAMWRKHMALGETAETEEVK 147
           SNYLFGLISVSLYAYVSYTFKLYGEMMLNLLVYVPVQFVGFAMWRKHMALGETAETEEVK
Sbjct: 63  SNYLFGLISVSLYAYVSYTFKLYGEMMLNLLVYVPVQFVGFAMWRKHMALGETAETEEVK 122

Query: 148 AKALTVRQWLLVVAASVVGTSVYIEWLHHLGSALPTLDGVTVVVSIVAQVLMILRYREQW 207
           AKALTVRQWLLVVAASVVGTSVYIEWLHHLGSALPTLDGVTVVVSIVAQVLMILRYREQW
Sbjct: 123 AKALTVRQWLLVVAASVVGTSVYIEWLHHLGSALPTLDGVTVVVSIVAQVLMILRYREQW 182

Query: 208 ALWIVVNILTISLWAVAWFKNGETSLPLLLMYVMYLCNSVYGYINWTKLVKRHSGQ 263
           ALWIVVNILTISLWAVAWFKNGETSLPLLLMYVMYLCNSVYGYINWTKLVKRHSGQ
Sbjct: 183 ALWIVVNILTISLWAVAWFKNGETSLPLLLMYVMYLCNSVYGYINWTKLVKRHSGQ 238


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

4QTN-A nrPDB                                                          419   e-118

Sequences not found previously or not previously below threshold:

4GAK-A nrPDB                                                           28   4.1  


CONVERGED!
>4QTN-A nrPDB
          Length = 244

 Score =  419 bits (1077), Expect = e-118,   Method: Composition-based stats.
 Identities = 236/236 (100%), Positives = 236/236 (100%)

Query: 28  SLAWWKRELFGGWTHFEAVWLLMFLGIQAVVFVFNPDSWLASVAAVTGILCVVFVGKGKI 87
           SLAWWKRELFGGWTHFEAVWLLMFLGIQAVVFVFNPDSWLASVAAVTGILCVVFVGKGKI
Sbjct: 3   SLAWWKRELFGGWTHFEAVWLLMFLGIQAVVFVFNPDSWLASVAAVTGILCVVFVGKGKI 62

Query: 88  SNYLFGLISVSLYAYVSYTFKLYGEMMLNLLVYVPVQFVGFAMWRKHMALGETAETEEVK 147
           SNYLFGLISVSLYAYVSYTFKLYGEMMLNLLVYVPVQFVGFAMWRKHMALGETAETEEVK
Sbjct: 63  SNYLFGLISVSLYAYVSYTFKLYGEMMLNLLVYVPVQFVGFAMWRKHMALGETAETEEVK 122

Query: 148 AKALTVRQWLLVVAASVVGTSVYIEWLHHLGSALPTLDGVTVVVSIVAQVLMILRYREQW 207
           AKALTVRQWLLVVAASVVGTSVYIEWLHHLGSALPTLDGVTVVVSIVAQVLMILRYREQW
Sbjct: 123 AKALTVRQWLLVVAASVVGTSVYIEWLHHLGSALPTLDGVTVVVSIVAQVLMILRYREQW 182

Query: 208 ALWIVVNILTISLWAVAWFKNGETSLPLLLMYVMYLCNSVYGYINWTKLVKRHSGQ 263
           ALWIVVNILTISLWAVAWFKNGETSLPLLLMYVMYLCNSVYGYINWTKLVKRHSGQ
Sbjct: 183 ALWIVVNILTISLWAVAWFKNGETSLPLLLMYVMYLCNSVYGYINWTKLVKRHSGQ 238


>4GAK-A nrPDB
          Length = 250

 Score = 28.1 bits (61), Expect = 4.1,   Method: Composition-based stats.
 Identities = 19/79 (24%), Positives = 34/79 (43%), Gaps = 7/79 (8%)

Query: 139 ETAETEEVKAKALTVRQWLLVVAASVVGTSVYIEWLHHLGSALPTLDGVTVVVSIVAQVL 198
           +TA      +K++ V  WL +     V    Y++WL      L  +D   V    +A++ 
Sbjct: 152 QTASFATAASKSVQV-GWLNIDQNQHVNNVAYVQWL------LEGVDSEIVQTREIAEID 204

Query: 199 MILRYREQWALWIVVNILT 217
           ++ R    W  W+ V  +T
Sbjct: 205 LVYRTESHWHDWLSVQSVT 223


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.327    0.139    0.451 

Lambda     K      H
   0.267   0.0454    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,789,123
Number of Sequences: 36641
Number of extensions: 655570
Number of successful extensions: 1642
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 1635
Number of HSP's gapped (non-prelim): 28
length of query: 263
length of database: 10,336,785
effective HSP length: 95
effective length of query: 168
effective length of database: 6,855,890
effective search space: 1151789520
effective search space used: 1151789520
T: 11
A: 40
X1: 16 ( 7.6 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 58 (26.8 bits)