BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= A4ZDL6 (134 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3A2E-A nrPDB 223 2e-59 6GRF-A nrPDB 52 7e-08 3TSY-A nrPDB 29 0.77 3SXN-A nrPDB 28 1.2 3UY5-A nrPDB 28 1.8 2Q28-A nrPDB 27 3.2 5OOK-A nrPDB 25 6.8 1E0X-A nrPDB 25 9.8 >3A2E-A nrPDB Length = 108 Score = 223 bits (568), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 108/108 (100%), Positives = 108/108 (100%) Query: 27 ANTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNTAFSGYDYKTSRAGSGGAPTAYGRA 86 ANTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNTAFSGYDYKTSRAGSGGAPTAYGRA Sbjct: 1 ANTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNTAFSGYDYKTSRAGSGGAPTAYGRA 60 Query: 87 TCKQSISQSDCTACLSNLVNRIFSICNNAIGARVQLVDCFIQYEQRSF 134 TCKQSISQSDCTACLSNLVNRIFSICNNAIGARVQLVDCFIQYEQRSF Sbjct: 61 TCKQSISQSDCTACLSNLVNRIFSICNNAIGARVQLVDCFIQYEQRSF 108 >6GRF-A nrPDB Length = 242 Score = 52.4 bits (124), Expect = 7e-08, Method: Compositional matrix adjust. Identities = 28/107 (26%), Positives = 51/107 (47%), Gaps = 3/107 (2%) Query: 31 FVSSACNTQKIPSGSPFNRNLRAMLADL---RQNTAFSGYDYKTSRAGSGGAPTAYGRAT 87 F+ C+ +K +PF N L+ + + +F+ + + S + +G Sbjct: 8 FIYGGCSPEKYTPNTPFESNRDTFLSSVVTSSSDASFNSFAVGNDSSSSSSSSAVFGLYQ 67 Query: 88 CKQSISQSDCTACLSNLVNRIFSICNNAIGARVQLVDCFIQYEQRSF 134 C+ + SDC+ C+ V++I IC + GA +QL CF++YE F Sbjct: 68 CRDDLRSSDCSKCIQTSVDQITLICPYSYGASLQLEGCFLRYETNDF 114 Score = 51.2 bits (121), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 30/102 (29%), Positives = 51/102 (50%), Gaps = 9/102 (8%) Query: 28 NTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNTAFSGYDYKTSRAGSGGAPTAYGRAT 87 +T+ C+++ + + F + +L+DL YK SR+G G A Sbjct: 119 DTSLRYKKCSSKSVENDYDFFKRRDDVLSDLESTQL----GYKVSRSG-----LVEGYAQ 169 Query: 88 CKQSISQSDCTACLSNLVNRIFSICNNAIGARVQLVDCFIQY 129 C +S SDCTACL+ V ++ ++C +A+ A V L C+ +Y Sbjct: 170 CVGDLSPSDCTACLAESVGKLKNLCGSAVAAEVYLAQCYARY 211 >3TSY-A nrPDB Length = 979 Score = 28.9 bits (63), Expect = 0.77, Method: Composition-based stats. Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 1/58 (1%) Query: 40 KIPSGSPFNRNLRAMLADLRQNTAFSGYDYKTSRAGSGGAPT-AYGRATCKQSISQSD 96 K PSG ++LRA LA+ + S +++ ++ G A A G AT + QSD Sbjct: 565 KAPSGKILRKDLRAKLANGLGSGMASVEEFRNAQRAKGPATILAIGTATPDHCVYQSD 622 >3SXN-A nrPDB Length = 422 Score = 28.1 bits (61), Expect = 1.2, Method: Composition-based stats. Identities = 17/45 (37%), Positives = 21/45 (46%), Gaps = 2/45 (4%) Query: 51 LRAMLADLRQNTAFSGYDYKTSRAGSGG--APTAYGRATCKQSIS 93 LRAM +L A +GY A GG YG AT +Q +S Sbjct: 117 LRAMYTELHDRIARAGYPLAVLTASEGGIYGRFGYGVATIEQHVS 161 >3UY5-A nrPDB Length = 428 Score = 27.7 bits (60), Expect = 1.8, Method: Composition-based stats. Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 3/35 (8%) Query: 51 LRAMLADLRQNTAFSGYDYKTSRAGSGGAPTAYGR 85 LRAM A+L + A SGY A GG YGR Sbjct: 123 LRAMCAELHRRIADSGYPVAALHASEGG---IYGR 154 >2Q28-A nrPDB Length = 564 Score = 26.6 bits (57), Expect = 3.2, Method: Composition-based stats. Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 8/45 (17%) Query: 26 AANTAFVSSACNTQKIPSGSPFN--------RNLRAMLADLRQNT 62 AA+T F+ Q+I S P +++ MLA+L+QNT Sbjct: 293 AADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELKQNT 337 >5OOK-A nrPDB Length = 162 Score = 25.4 bits (54), Expect = 6.8, Method: Compositional matrix adjust. Identities = 14/38 (36%), Positives = 18/38 (47%) Query: 55 LADLRQNTAFSGYDYKTSRAGSGGAPTAYGRATCKQSI 92 LAD N + + Y T GS A T G+A C + I Sbjct: 51 LADGAANAVYQKFPYTTQMTGSNYASTPEGKAKCVRDI 88 >1E0X-A nrPDB Length = 313 Score = 25.0 bits (53), Expect = 9.8, Method: Compositional matrix adjust. Identities = 16/38 (42%), Positives = 18/38 (47%), Gaps = 4/38 (10%) Query: 43 SGSPFNRNLRAMLADLRQNTAFSGYDYKTSRAGSGGAP 80 SGSP+N N R L QN A G D + GAP Sbjct: 210 SGSPYNSNFRTTL----QNFAALGVDVAITELDIQGAP 243 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3A2E-A nrPDB 153 2e-38 6GRF-A nrPDB 146 3e-36 Sequences not found previously or not previously below threshold: 2Q28-A nrPDB 28 1.5 5OOK-A nrPDB 27 3.2 1IT0-A nrPDB 26 6.5 2G4F-A nrPDB 25 7.2 CONVERGED! >3A2E-A nrPDB Length = 108 Score = 153 bits (386), Expect = 2e-38, Method: Composition-based stats. Identities = 108/108 (100%), Positives = 108/108 (100%) Query: 27 ANTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNTAFSGYDYKTSRAGSGGAPTAYGRA 86 ANTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNTAFSGYDYKTSRAGSGGAPTAYGRA Sbjct: 1 ANTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNTAFSGYDYKTSRAGSGGAPTAYGRA 60 Query: 87 TCKQSISQSDCTACLSNLVNRIFSICNNAIGARVQLVDCFIQYEQRSF 134 TCKQSISQSDCTACLSNLVNRIFSICNNAIGARVQLVDCFIQYEQRSF Sbjct: 61 TCKQSISQSDCTACLSNLVNRIFSICNNAIGARVQLVDCFIQYEQRSF 108 >6GRF-A nrPDB Length = 242 Score = 146 bits (369), Expect = 3e-36, Method: Composition-based stats. Identities = 28/107 (26%), Positives = 51/107 (47%), Gaps = 3/107 (2%) Query: 31 FVSSACNTQKIPSGSPFNRNLRAMLADL---RQNTAFSGYDYKTSRAGSGGAPTAYGRAT 87 F+ C+ +K +PF N L+ + + +F+ + + S + +G Sbjct: 8 FIYGGCSPEKYTPNTPFESNRDTFLSSVVTSSSDASFNSFAVGNDSSSSSSSSAVFGLYQ 67 Query: 88 CKQSISQSDCTACLSNLVNRIFSICNNAIGARVQLVDCFIQYEQRSF 134 C+ + SDC+ C+ V++I IC + GA +QL CF++YE F Sbjct: 68 CRDDLRSSDCSKCIQTSVDQITLICPYSYGASLQLEGCFLRYETNDF 114 Score = 55.5 bits (132), Expect = 7e-09, Method: Composition-based stats. Identities = 30/102 (29%), Positives = 52/102 (50%), Gaps = 9/102 (8%) Query: 28 NTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNTAFSGYDYKTSRAGSGGAPTAYGRAT 87 +T+ C+++ + + F + +L+DL + YK SR+G G A Sbjct: 119 DTSLRYKKCSSKSVENDYDFFKRRDDVLSDLES----TQLGYKVSRSG-----LVEGYAQ 169 Query: 88 CKQSISQSDCTACLSNLVNRIFSICNNAIGARVQLVDCFIQY 129 C +S SDCTACL+ V ++ ++C +A+ A V L C+ +Y Sbjct: 170 CVGDLSPSDCTACLAESVGKLKNLCGSAVAAEVYLAQCYARY 211 >2Q28-A nrPDB Length = 564 Score = 27.8 bits (60), Expect = 1.5, Method: Composition-based stats. Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 8/45 (17%) Query: 26 AANTAFVSSACNTQKIPSGSP--------FNRNLRAMLADLRQNT 62 AA+T F+ Q+I S P +++ MLA+L+QNT Sbjct: 293 AADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELKQNT 337 >5OOK-A nrPDB Length = 162 Score = 26.6 bits (57), Expect = 3.2, Method: Composition-based stats. Identities = 14/38 (36%), Positives = 18/38 (47%) Query: 55 LADLRQNTAFSGYDYKTSRAGSGGAPTAYGRATCKQSI 92 LAD N + + Y T GS A T G+A C + I Sbjct: 51 LADGAANAVYQKFPYTTQMTGSNYASTPEGKAKCVRDI 88 >1IT0-A nrPDB Length = 436 Score = 25.8 bits (55), Expect = 6.5, Method: Composition-based stats. Identities = 14/44 (31%), Positives = 21/44 (47%), Gaps = 3/44 (6%) Query: 41 IPSGSPFNRNLRAML---ADLRQNTAFSGYDYKTSRAGSGGAPT 81 SGSP+N N R L A L + A + D + + + + A T Sbjct: 208 FNSGSPYNSNFRTTLQNFAALGVDVAITELDIQGASSSTYAAVT 251 >2G4F-A nrPDB Length = 313 Score = 25.4 bits (54), Expect = 7.2, Method: Composition-based stats. Identities = 14/44 (31%), Positives = 21/44 (47%), Gaps = 3/44 (6%) Query: 41 IPSGSPFNRNLRAML---ADLRQNTAFSGYDYKTSRAGSGGAPT 81 SGSP+N N R L A L + A + D + + + + A T Sbjct: 208 FNSGSPYNSNFRTTLQNFAALGVDVAITELDIQGASSSTYAAVT 251 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.131 0.345 Lambda K H 0.267 0.0394 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,036,557 Number of Sequences: 36641 Number of extensions: 123849 Number of successful extensions: 385 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 370 Number of HSP's gapped (non-prelim): 28 length of query: 134 length of database: 10,336,785 effective HSP length: 86 effective length of query: 48 effective length of database: 7,185,659 effective search space: 344911632 effective search space used: 344911632 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.2 bits) S2: 53 (25.1 bits)