BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= A0A0H3M3S8 (236 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3MHA-A nrPDB 397 e-111 4QA8-A nrPDB 92 2e-19 2BYO-A nrPDB 87 5e-18 1JRP-A nrPDB 30 1.1 4LRH-A nrPDB 29 1.3 6MHW-A nrPDB 28 2.8 2YFT-A nrPDB 28 3.9 4DZA-A nrPDB 27 6.0 2DYM-A nrPDB 27 6.1 >3MHA-A nrPDB Length = 203 Score = 397 bits (1019), Expect = e-111, Method: Compositional matrix adjust. Identities = 196/196 (100%), Positives = 196/196 (100%) Query: 36 GPLPDAKPLVEEATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNPTAATGNVKLTL 95 GPLPDAKPLVEEATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNPTAATGNVKLTL Sbjct: 8 GPLPDAKPLVEEATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNPTAATGNVKLTL 67 Query: 96 GGSDIDADFVVFDGILYATLTPNQWSDFGPAADIYDPAQVLNPDTGLANVLANFADAKAE 155 GGSDIDADFVVFDGILYATLTPNQWSDFGPAADIYDPAQVLNPDTGLANVLANFADAKAE Sbjct: 68 GGSDIDADFVVFDGILYATLTPNQWSDFGPAADIYDPAQVLNPDTGLANVLANFADAKAE 127 Query: 156 GRDTINGQNTIRISGKVSAQAVNQIAPPFNATQPVPATVWIQETGDHQLAQAQLDRGSGN 215 GRDTINGQNTIRISGKVSAQAVNQIAPPFNATQPVPATVWIQETGDHQLAQAQLDRGSGN Sbjct: 128 GRDTINGQNTIRISGKVSAQAVNQIAPPFNATQPVPATVWIQETGDHQLAQAQLDRGSGN 187 Query: 216 SVQMTLSKWGEKVQVT 231 SVQMTLSKWGEKVQVT Sbjct: 188 SVQMTLSKWGEKVQVT 203 >4QA8-A nrPDB Length = 229 Score = 92.4 bits (228), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 62/206 (30%), Positives = 106/206 (51%), Gaps = 15/206 (7%) Query: 40 DAKPLVEEATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNPTA---ATGNVKLTLG 96 +A+ ++++++ TK L S H+V+TVN + L +++ D+T P A GN K+ + Sbjct: 25 EAQQILQDSSKATKGLHSVHVVVTVN-NLSTLPFESVDADVTNQPQGNGQAVGNAKVRMK 83 Query: 97 GSD--IDADFVVFDGILYATLTPNQWSDFGPAADIYDPAQVLNPDTGLANVLANFADAKA 154 + + +F+V + +Y T + GPA IYDP +L+ D GL V+ + Sbjct: 84 PNTPVVATEFLVTNKTMY-TKRGGDYVSVGPAEKIYDPGIILDKDRGLGAVVGQVQNPTI 142 Query: 155 EGRDTINGQNTIRISGKVSAQAVNQIAPPF-NATQPVPATVWIQETGDH------QLAQA 207 +GRD I+G T+++SG + A ++ I P +P T+WI +T L + Sbjct: 143 QGRDAIDGLATVKVSGTIDAAVIDPIVPQLGKGGGRLPITLWIVDTNASTPAPAANLVRM 202 Query: 208 QLDRGSGNSVQMTLSKWGEKVQVTKP 233 +D+ GN V +TLS WG V + P Sbjct: 203 VIDKDQGN-VDITLSNWGAPVTIPNP 227 >2BYO-A nrPDB Length = 207 Score = 87.4 bits (215), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 61/195 (31%), Positives = 98/195 (50%), Gaps = 25/195 (12%) Query: 45 VEEATAQTKALKSAHMVLTVNGKIPGL-SLKTLSGDLTTNPTAATG--------NVKLTL 95 + ++ TK L S H+ + GK+ L + + D+ NP AA G V + Sbjct: 28 IRQSLDATKGLTSVHVAVRTTGKVDSLLGITSADVDVRANPLAAKGVCTYNDEQGVPFRV 87 Query: 96 GGSDIDADFVVFDGILYATLTPNQWSDFGPAADIYDPAQVLNPDTGLANVLANFADAKAE 155 G +I FD WS+ G +++ ++VL+P G+ +L+ + +A+ Sbjct: 88 QGDNISVKL--FD----------DWSNLGSISELS-TSRVLDPAAGVTQLLSGVTNLQAQ 134 Query: 156 GRDTINGQNTIRISGKVSAQAVNQIAPPFNATQPVPATVWIQETGDHQLAQAQLDRGSGN 215 G + I+G +T +I+G + A +V + P A PATVWI + G H L +A +D GSG Sbjct: 135 GTEVIDGISTTKITGTIPASSVKMLDP--GAKSARPATVWIAQDGSHHLVRASIDLGSG- 191 Query: 216 SVQMTLSKWGEKVQV 230 S+Q+T SKW E V V Sbjct: 192 SIQLTQSKWNEPVNV 206 >1JRP-A nrPDB Length = 462 Score = 29.6 bits (65), Expect = 1.1, Method: Compositional matrix adjust. Identities = 15/42 (35%), Positives = 21/42 (50%) Query: 69 PGLSLKTLSGDLTTNPTAATGNVKLTLGGSDIDADFVVFDGI 110 PGL LS + +A G + LTL GS I+ + F G+ Sbjct: 346 PGLRCYKLSKRFDQDISAVCGCLNLTLKGSKIETARIAFGGM 387 >4LRH-A nrPDB Length = 218 Score = 29.3 bits (64), Expect = 1.3, Method: Compositional matrix adjust. Identities = 29/118 (24%), Positives = 40/118 (33%), Gaps = 10/118 (8%) Query: 108 DGILYATLTPNQWSDFGPAADIYDPA----QVLNPDTGLANVLANFADAKAEGRDTINGQ 163 D LY +PN GP D + +VLN + + D + N Sbjct: 81 DTCLYE-CSPN----LGPWIQQVDQSWRKERVLNVPLCKEDCEQWWEDCRTSYTCKSNWH 135 Query: 164 NTIRISGKVSAQAVNQIAPPFNATQPVPATVWIQETGDHQLAQAQLDRGSGNSVQMTL 221 + + AV PF+ P P TV E H + RGSG +QM Sbjct: 136 KGWNWTSGFNKCAVGAACQPFHFYFPTP-TVLCNEIWTHSYKVSNYSRGSGRCIQMWF 192 >6MHW-A nrPDB Length = 826 Score = 28.5 bits (62), Expect = 2.8, Method: Composition-based stats. Identities = 21/75 (28%), Positives = 34/75 (45%), Gaps = 9/75 (12%) Query: 31 SKPSGGP--LPDAKPLVEEATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNPTAAT 88 + PSG P LP+ +P T KSAH L + G P ++ S + + P + Sbjct: 18 AAPSGNPAILPEKRP-----AEITPTKKSAHFFLEIEGFEPNPTVAKTSPPVFSKPMDS- 71 Query: 89 GNVKLTLGGSDIDAD 103 N++ + G+ D D Sbjct: 72 -NIRQCISGNCDDMD 85 >2YFT-A nrPDB Length = 571 Score = 27.7 bits (60), Expect = 3.9, Method: Compositional matrix adjust. Identities = 15/44 (34%), Positives = 22/44 (50%), Gaps = 2/44 (4%) Query: 100 IDADFVVFDGILYATLTPNQWSDFGPAAD--IYDPAQVLNPDTG 141 +D D +VF+G Y T +QW + AD A V++ D G Sbjct: 248 VDNDHIVFEGDGYHYQTYDQWKETNKGADNIAMRDAHVIDDDNG 291 >4DZA-A nrPDB Length = 407 Score = 27.3 bits (59), Expect = 6.0, Method: Compositional matrix adjust. Identities = 25/101 (24%), Positives = 39/101 (38%), Gaps = 6/101 (5%) Query: 130 YDPAQVLNPDTGLANVLANFADAKAE-----GRDTINGQNTIRISGKVSAQAVNQIAPPF 184 YD L D+ LAN+ +AD G I GQ + + GK S V Sbjct: 299 YDRTYTLKRDSVLANIPVGYADGYRRVFSNAGHALIAGQR-VPVLGKTSMNTVIVDITSL 357 Query: 185 NATQPVPATVWIQETGDHQLAQAQLDRGSGNSVQMTLSKWG 225 N +P V+ + G+ ++ +++ SG WG Sbjct: 358 NNIKPGDEVVFFGKQGNSEITAEEIEDISGALFTEMSILWG 398 >2DYM-A nrPDB Length = 297 Score = 27.3 bits (59), Expect = 6.1, Method: Compositional matrix adjust. Identities = 22/89 (24%), Positives = 38/89 (42%), Gaps = 12/89 (13%) Query: 39 PDAKPLVEEATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNPTAATGN-VKLTLGG 97 P PL+ + + + + + +++ G P +LK + GD+ GN V + G Sbjct: 200 PRHIPLIIQTSRTSGTFRISQPTISMTGVNP--TLKDIEGDILDVKEGINGNDVMVICQG 257 Query: 98 SDIDADFVV---------FDGILYATLTP 117 +I ++ FDG LY TL P Sbjct: 258 IEIPWHMLLYDLYSKLRSFDGFLYITLVP 286 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3MHA-A nrPDB 274 2e-74 4QA8-A nrPDB 215 1e-56 2BYO-A nrPDB 183 4e-47 Sequences not found previously or not previously below threshold: 5L8R-B nrPDB 30 1.1 6IGZ-B nrPDB 29 1.8 6HQB-A nrPDB 28 3.3 4FE1-A nrPDB 28 3.3 5ZGB-A nrPDB 28 3.9 2ZPA-A nrPDB 28 4.1 6IJO-A nrPDB 28 4.3 1R6V-A nrPDB 27 6.9 4LX4-A nrPDB 27 7.6 CONVERGED! >3MHA-A nrPDB Length = 203 Score = 274 bits (700), Expect = 2e-74, Method: Composition-based stats. Identities = 196/196 (100%), Positives = 196/196 (100%) Query: 36 GPLPDAKPLVEEATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNPTAATGNVKLTL 95 GPLPDAKPLVEEATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNPTAATGNVKLTL Sbjct: 8 GPLPDAKPLVEEATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNPTAATGNVKLTL 67 Query: 96 GGSDIDADFVVFDGILYATLTPNQWSDFGPAADIYDPAQVLNPDTGLANVLANFADAKAE 155 GGSDIDADFVVFDGILYATLTPNQWSDFGPAADIYDPAQVLNPDTGLANVLANFADAKAE Sbjct: 68 GGSDIDADFVVFDGILYATLTPNQWSDFGPAADIYDPAQVLNPDTGLANVLANFADAKAE 127 Query: 156 GRDTINGQNTIRISGKVSAQAVNQIAPPFNATQPVPATVWIQETGDHQLAQAQLDRGSGN 215 GRDTINGQNTIRISGKVSAQAVNQIAPPFNATQPVPATVWIQETGDHQLAQAQLDRGSGN Sbjct: 128 GRDTINGQNTIRISGKVSAQAVNQIAPPFNATQPVPATVWIQETGDHQLAQAQLDRGSGN 187 Query: 216 SVQMTLSKWGEKVQVT 231 SVQMTLSKWGEKVQVT Sbjct: 188 SVQMTLSKWGEKVQVT 203 >4QA8-A nrPDB Length = 229 Score = 215 bits (548), Expect = 1e-56, Method: Composition-based stats. Identities = 62/206 (30%), Positives = 106/206 (51%), Gaps = 15/206 (7%) Query: 40 DAKPLVEEATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNPTA---ATGNVKLTLG 96 +A+ ++++++ TK L S H+V+TVN + L +++ D+T P A GN K+ + Sbjct: 25 EAQQILQDSSKATKGLHSVHVVVTVN-NLSTLPFESVDADVTNQPQGNGQAVGNAKVRMK 83 Query: 97 GSD--IDADFVVFDGILYATLTPNQWSDFGPAADIYDPAQVLNPDTGLANVLANFADAKA 154 + + +F+V + +Y T + GPA IYDP +L+ D GL V+ + Sbjct: 84 PNTPVVATEFLVTNKTMY-TKRGGDYVSVGPAEKIYDPGIILDKDRGLGAVVGQVQNPTI 142 Query: 155 EGRDTINGQNTIRISGKVSAQAVNQIAPPFN-ATQPVPATVWIQETGDH------QLAQA 207 +GRD I+G T+++SG + A ++ I P +P T+WI +T L + Sbjct: 143 QGRDAIDGLATVKVSGTIDAAVIDPIVPQLGKGGGRLPITLWIVDTNASTPAPAANLVRM 202 Query: 208 QLDRGSGNSVQMTLSKWGEKVQVTKP 233 +D+ GN V +TLS WG V + P Sbjct: 203 VIDKDQGN-VDITLSNWGAPVTIPNP 227 >2BYO-A nrPDB Length = 207 Score = 183 bits (465), Expect = 4e-47, Method: Composition-based stats. Identities = 59/195 (30%), Positives = 97/195 (49%), Gaps = 25/195 (12%) Query: 45 VEEATAQTKALKSAHMVLTVNGKIPGL-SLKTLSGDLTTNPTAATG--------NVKLTL 95 + ++ TK L S H+ + GK+ L + + D+ NP AA G V + Sbjct: 28 IRQSLDATKGLTSVHVAVRTTGKVDSLLGITSADVDVRANPLAAKGVCTYNDEQGVPFRV 87 Query: 96 GGSDIDADFVVFDGILYATLTPNQWSDFGPAADIYDPAQVLNPDTGLANVLANFADAKAE 155 G +I + WS+ G +++ ++VL+P G+ +L+ + +A+ Sbjct: 88 QGDNISVKLF------------DDWSNLGSISELS-TSRVLDPAAGVTQLLSGVTNLQAQ 134 Query: 156 GRDTINGQNTIRISGKVSAQAVNQIAPPFNATQPVPATVWIQETGDHQLAQAQLDRGSGN 215 G + I+G +T +I+G + A +V + P A PATVWI + G H L +A +D GSG Sbjct: 135 GTEVIDGISTTKITGTIPASSVKMLDP--GAKSARPATVWIAQDGSHHLVRASIDLGSG- 191 Query: 216 SVQMTLSKWGEKVQV 230 S+Q+T SKW E V V Sbjct: 192 SIQLTQSKWNEPVNV 206 >5L8R-B nrPDB Length = 734 Score = 29.7 bits (65), Expect = 1.1, Method: Composition-based stats. Identities = 17/76 (22%), Positives = 28/76 (36%), Gaps = 8/76 (10%) Query: 135 VLNPDTGLANVLANFADAKAEGRDTINGQ-NTIRISGKVSAQAVNQIAPPFNATQPVPAT 193 VL GL + + A+ D+ N +T + +G I P + Sbjct: 215 VLPHPQGLGPLFTGQWNLYAQNPDSSNHLFSTSQGAGT-------AILTLLGGFHPQTQS 267 Query: 194 VWIQETGDHQLAQAQL 209 +W+ + H LA A L Sbjct: 268 LWLTDMAHHHLAIAIL 283 >6IGZ-B nrPDB Length = 734 Score = 29.0 bits (63), Expect = 1.8, Method: Composition-based stats. Identities = 19/75 (25%), Positives = 27/75 (36%), Gaps = 6/75 (8%) Query: 135 VLNPDTGLANVLANFADAKAEGRDTINGQNTIRISGKVSAQAVNQIAPPFNATQPVPATV 194 +L GL A A+ DTIN + +G A I P ++ Sbjct: 215 ILPHPLGLKPFFTGNWSAYAQNPDTINHIFSTN-TGSGDA-----ILTFLGGFHPQTQSL 268 Query: 195 WIQETGDHQLAQAQL 209 W+ + H LA A L Sbjct: 269 WLTDMAHHHLAIAVL 283 >6HQB-A nrPDB Length = 751 Score = 28.2 bits (61), Expect = 3.3, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 19/41 (46%), Gaps = 3/41 (7%) Query: 185 NATQPVPATVWIQETGDHQLAQAQLDRGSGNSVQMTLSKWG 225 PV +W+ +T H LA A L +G+ M + WG Sbjct: 279 GGLNPVTGGLWLSDTAHHHLAIAVLFIIAGH---MYRTNWG 316 >4FE1-A nrPDB Length = 755 Score = 28.2 bits (61), Expect = 3.3, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 19/41 (46%), Gaps = 3/41 (7%) Query: 185 NATQPVPATVWIQETGDHQLAQAQLDRGSGNSVQMTLSKWG 225 PV +W+ +T H LA A L +G+ M + WG Sbjct: 283 GGLNPVTGGLWLSDTAHHHLAIAVLFIIAGH---MYRTNWG 320 >5ZGB-A nrPDB Length = 748 Score = 27.8 bits (60), Expect = 3.9, Method: Composition-based stats. Identities = 12/41 (29%), Positives = 18/41 (43%), Gaps = 3/41 (7%) Query: 185 NATQPVPATVWIQETGDHQLAQAQLDRGSGNSVQMTLSKWG 225 PV +W+ + H LA A L +G+ M + WG Sbjct: 276 GGLSPVTGGLWLTDVAHHHLAIAVLFLVAGH---MYRTNWG 313 >2ZPA-A nrPDB Length = 671 Score = 27.8 bits (60), Expect = 4.1, Method: Composition-based stats. Identities = 20/108 (18%), Positives = 38/108 (35%), Gaps = 19/108 (17%) Query: 34 SGGPLPDAKPLVEE---------ATAQTKALKSAHMVLTVNGKIPGLSLKTLSGDLTTNP 84 +G P P+ + L+++ A + + + + +I G ++ T +T+ Sbjct: 173 TGAPQPEQQQLLKQLMTMPPGVAAVTAARGRGKSALAGQLISRIAGRAIVTAPAKASTDV 232 Query: 85 TAATGNVKLTLGGSDIDADFVVFDGILYATLTPNQWSDFGPAADIYDP 132 A K F+ D +L A+ W AA I P Sbjct: 233 LAQFAGEKFR---------FIAPDALL-ASDEQADWLVVDEAAAIPAP 270 >6IJO-A nrPDB Length = 751 Score = 27.8 bits (60), Expect = 4.3, Method: Composition-based stats. Identities = 12/41 (29%), Positives = 19/41 (46%), Gaps = 3/41 (7%) Query: 185 NATQPVPATVWIQETGDHQLAQAQLDRGSGNSVQMTLSKWG 225 PV +W+ +T H +A A L +G+ M + WG Sbjct: 280 GGLNPVTGGLWLSDTAHHHVAIAVLFLVAGH---MYRTNWG 317 >1R6V-A nrPDB Length = 671 Score = 27.0 bits (58), Expect = 6.9, Method: Composition-based stats. Identities = 16/59 (27%), Positives = 24/59 (40%), Gaps = 5/59 (8%) Query: 141 GLANVLANFADAKAEGRDTINGQNTIRISGKVSAQAVNQIAPPFN----ATQPVPATVW 195 G+ +L D +G T+NG++ I + G + A I F T P VW Sbjct: 608 GIQTLLPAATDITIQGTVTLNGED-IPVYGVLKAGTTWTIIDDFGGLNLGTDSQPIYVW 665 >4LX4-A nrPDB Length = 330 Score = 27.0 bits (58), Expect = 7.6, Method: Composition-based stats. Identities = 11/32 (34%), Positives = 14/32 (43%) Query: 169 SGKVSAQAVNQIAPPFNATQPVPATVWIQETG 200 SGK AQ I P + P W+Q+ G Sbjct: 214 SGKYMAQTSRNIDPMIGVERARPFIEWLQKHG 245 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.126 0.334 Lambda K H 0.267 0.0387 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,266,610 Number of Sequences: 36641 Number of extensions: 296151 Number of successful extensions: 630 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 19 Number of HSP's that attempted gapping in prelim test: 604 Number of HSP's gapped (non-prelim): 27 length of query: 236 length of database: 10,336,785 effective HSP length: 94 effective length of query: 142 effective length of database: 6,892,531 effective search space: 978739402 effective search space used: 978739402 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 57 (26.6 bits)